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AU2001288019A1 - Nucleic acid constructs and cells, and methods utilizing same for modifying the electrophysiological function of excitable tissues - Google Patents

Nucleic acid constructs and cells, and methods utilizing same for modifying the electrophysiological function of excitable tissues

Info

Publication number
AU2001288019A1
AU2001288019A1 AU2001288019A AU8801901A AU2001288019A1 AU 2001288019 A1 AU2001288019 A1 AU 2001288019A1 AU 2001288019 A AU2001288019 A AU 2001288019A AU 8801901 A AU8801901 A AU 8801901A AU 2001288019 A1 AU2001288019 A1 AU 2001288019A1
Authority
AU
Australia
Prior art keywords
modifying
cells
nucleic acid
acid constructs
methods utilizing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001288019A
Inventor
Yair Feld
Meira Frank
Lior Gepstein
Shimon Marom
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Technion Research and Development Foundation Ltd
Original Assignee
Technion Research and Development Foundation Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Technion Research and Development Foundation Ltd filed Critical Technion Research and Development Foundation Ltd
Publication of AU2001288019A1 publication Critical patent/AU2001288019A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1329—Cardiomyocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/99—Coculture with; Conditioned medium produced by genetically modified cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Rheumatology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Cardiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention concerns cells expressing an endogenous ion channel/transporter polypeptide for the treatment of an excitable tissue disorder in a subject in need thereof.
AU2001288019A 2000-10-20 2001-09-05 Nucleic acid constructs and cells, and methods utilizing same for modifying the electrophysiological function of excitable tissues Abandoned AU2001288019A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/691,889 US7294333B1 (en) 2000-10-20 2000-10-20 Nucleic acid constructs and cells, and methods utilizing same for modifying the electrophysiological function of excitable tissues
US09/691,889 2000-10-20
PCT/IL2001/000833 WO2002033111A2 (en) 2000-10-20 2001-09-05 Nucleic acid constructs and cells, and methods utilizing same for modifying the electrophysiological function of excitable tissues

Publications (1)

Publication Number Publication Date
AU2001288019A1 true AU2001288019A1 (en) 2002-04-29

Family

ID=24778375

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001288019A Abandoned AU2001288019A1 (en) 2000-10-20 2001-09-05 Nucleic acid constructs and cells, and methods utilizing same for modifying the electrophysiological function of excitable tissues

Country Status (7)

Country Link
US (2) US7294333B1 (en)
EP (3) EP2385056B1 (en)
AT (1) ATE403664T1 (en)
AU (1) AU2001288019A1 (en)
CA (1) CA2426460A1 (en)
DE (1) DE60135237D1 (en)
WO (1) WO2002033111A2 (en)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001288807B2 (en) * 2000-09-06 2007-07-19 Johns Hopkins University Cardiac arrhythmia treatment methods
US7491385B2 (en) * 2001-09-05 2009-02-17 Genegrafts Ltd. Nucleic acid constructs and cells, and methods utilizing same for modifying the electrophysiological function of excitable tissues
BR0214018A (en) 2001-11-08 2005-04-19 Univ California Methods and compositions for the correction of cardiac conduction disorders
WO2004030630A2 (en) 2002-10-02 2004-04-15 The Johns Hopkins University Focal calcium channel modulation
US20050002914A1 (en) * 2003-01-15 2005-01-06 Rosen Michael R. Mesenchymal stem cells as a vehicle for ion channel transfer in syncytial structures
US20040214182A1 (en) * 2003-04-25 2004-10-28 Vinod Sharma Genetic modification of targeted regions of the cardiac conduction system
US8013133B2 (en) 2003-04-25 2011-09-06 Medtronic, Inc. Genetic modification of targeted regions of the cardiac conduction system
US20070021375A1 (en) 2003-04-25 2007-01-25 Vinod Sharma Genetic modification of targeted regions of the cardiac conduction system
US20080260705A1 (en) * 2004-03-22 2008-10-23 The Johns Hopkins University Preventing Arrhythmias Associated with Cell Transplantation
US7993906B2 (en) * 2004-05-28 2011-08-09 The Board Of Trustees Of The Leland Stanford Junior University Closed-loop electrical stimulation system for cell cultures
US8565898B2 (en) * 2005-04-28 2013-10-22 Medtronic, Inc. Rate control during AF using cellular intervention to modulate AV node
EP1973466B1 (en) 2006-01-19 2021-01-06 The General Hospital Corporation Ballon imaging catheter
EP2309221A1 (en) 2006-02-24 2011-04-13 The General Hospital Corporation Methods and systems for performing angle-resolved fourier-domain optical coherence tomography
WO2008049118A2 (en) 2006-10-19 2008-04-24 The General Hospital Corporation Apparatus and method for obtaining and providing imaging information associated with at least one portion of a sample and effecting such portion(s)
US20100015105A1 (en) * 2006-12-27 2010-01-21 Shimon Marom Method of treating schizophrenia
US9150832B2 (en) * 2007-05-08 2015-10-06 Cardiac Pacemakers, Inc. Cell training for local field stimulation
US20080280341A1 (en) * 2007-05-08 2008-11-13 Kenknight Bruce System And Method For Local Field Stimulation
US8311620B2 (en) * 2007-08-22 2012-11-13 Cardiac Pacemakers, Inc. Methods and apparatus to treat and prevent atrial tachyarrhythmias
US20110144028A1 (en) * 2009-12-11 2011-06-16 Vinod Sharma Modulating cellular electrophysiology
US8639323B2 (en) 2010-11-01 2014-01-28 Medtronic, Inc. System and apparatus to monitor biopacemaker maturation

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5683912A (en) 1994-07-21 1997-11-04 The Salk Institute For Biological Studies Cloning and expression of a novel acetylcholine-gated ion channel receptor subunit
US5602301A (en) * 1993-11-16 1997-02-11 Indiana University Foundation Non-human mammal having a graft and methods of delivering protein to myocardial tissue
US5837220A (en) 1995-09-19 1998-11-17 Merck & Co., Inc. Method for analyzing the immunosuppressant activity of ion channel blockers using the mini-pig
US5955259A (en) 1996-12-19 1999-09-21 Brandeis University Method for assessing modulation of potassium ion channel activity
US6099832A (en) 1997-05-28 2000-08-08 Genzyme Corporation Transplants for myocardial scars
US6110459A (en) 1997-05-28 2000-08-29 Mickle; Donald A. G. Transplants for myocardial scars and methods and cellular preparations
US5986081A (en) 1997-10-22 1999-11-16 Wisconsin Alumni Research Foundation Polynucleotides encoding herg-3
US6913925B1 (en) * 1998-08-12 2005-07-05 Signal Pharmaceuticals Llc Human mesencephalon cell lines and methods of use therefor
AU6501499A (en) * 1998-09-29 2000-04-17 Johns Hopkins University, The Inducible genetic suppression of cellular excitability
AU775525B2 (en) * 1999-01-22 2004-08-05 Auckland Technology Enabling Corporation Limited Vaccine-mediated treatment of neurological disorders
ES2338520T3 (en) 1999-04-15 2010-05-10 The University Of Utah Research Foundation GENES RELATED TO MINK, TRAINING OF POTASSIUM CHANNELS AND ASSOCIATION WITH CARDIAC ARRITMIES.
AU2001288807B2 (en) * 2000-09-06 2007-07-19 Johns Hopkins University Cardiac arrhythmia treatment methods
JP4279558B2 (en) 2001-04-27 2009-06-17 ジョンズ・ホプキンス・ユニバーシティ Biological pacemaker

Also Published As

Publication number Publication date
WO2002033111A2 (en) 2002-04-25
WO2002033111A3 (en) 2002-12-19
EP2385056A2 (en) 2011-11-09
ATE403664T1 (en) 2008-08-15
EP2385056A3 (en) 2012-02-01
EP1326879A2 (en) 2003-07-16
US7294333B1 (en) 2007-11-13
US20040029148A1 (en) 2004-02-12
EP1326879B1 (en) 2008-08-06
EP1326879A4 (en) 2004-09-22
CA2426460A1 (en) 2002-04-25
DE60135237D1 (en) 2008-09-18
EP2166017A1 (en) 2010-03-24
EP2385056B1 (en) 2015-06-10

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