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AU2001287712A1 - Method for the specific determination of DNA -sequences by means of parallel amplification - Google Patents

Method for the specific determination of DNA -sequences by means of parallel amplification

Info

Publication number
AU2001287712A1
AU2001287712A1 AU2001287712A AU8771201A AU2001287712A1 AU 2001287712 A1 AU2001287712 A1 AU 2001287712A1 AU 2001287712 A AU2001287712 A AU 2001287712A AU 8771201 A AU8771201 A AU 8771201A AU 2001287712 A1 AU2001287712 A1 AU 2001287712A1
Authority
AU
Australia
Prior art keywords
dna
solid phase
primers
sequences
specific determination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001287712A
Inventor
Stephan Schneider
Patric Zeltz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biochip Technologies GmbH
Original Assignee
Biochip Technologies GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biochip Technologies GmbH filed Critical Biochip Technologies GmbH
Publication of AU2001287712A1 publication Critical patent/AU2001287712A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Specific determination of DNA sequences (I), comprising parallel amplification in a combined liquid/solid phase microarray system, using nested polymerase chain reaction (PCR), with x primer sets (x = number of (I) being determined), each of at least three primers, is new. Specific determination of DNA sequences (I), comprising parallel amplification in a combined liquid/solid phase microarray system, using nested polymerase chain reaction (PCR), with x primer sets (x = number of (I) being determined), each of at least three primers, is new. Each primer set comprises: (a) two outer primers (P1, P2) that hybridize upstream and downstream of the target DNA (A) being amplified; and (b) an internal primer (P3) that hybridizes to (A) and can form an extension product (EP). The outer primers are present in the liquid phase, at an excess relative to P3, and P3 are irreversibly bound to a solid phase, forming a microarray of x spaced apart and defined positions. Determination is based on detecting an EP from P3 at defined array positions. Independent claims are also included for the following: (1) determining point mutations by the novel method; (2) determining the sequence of (unknown) partial sequences of DNA by the new method; and (3) solid phase DNA array of P3.
AU2001287712A 2000-09-05 2001-09-04 Method for the specific determination of DNA -sequences by means of parallel amplification Abandoned AU2001287712A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP00119182A EP1186669B1 (en) 2000-09-05 2000-09-05 Method for specific detection of DNS sequences using parallel amplification
EP00119182 2000-09-05
PCT/EP2001/010160 WO2002020833A2 (en) 2000-09-05 2001-09-04 Method for the specific determination of dna -sequences by means of parallel amplification

Publications (1)

Publication Number Publication Date
AU2001287712A1 true AU2001287712A1 (en) 2002-03-22

Family

ID=8169755

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001287712A Abandoned AU2001287712A1 (en) 2000-09-05 2001-09-04 Method for the specific determination of DNA -sequences by means of parallel amplification

Country Status (7)

Country Link
US (1) US7611871B2 (en)
EP (1) EP1186669B1 (en)
JP (1) JP2004508053A (en)
AT (1) ATE316581T1 (en)
AU (1) AU2001287712A1 (en)
DE (1) DE50012114D1 (en)
WO (1) WO2002020833A2 (en)

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* Cited by examiner, † Cited by third party
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ATE316581T1 (en) 2000-09-05 2006-02-15 Zeltz Patrick Dr METHOD FOR THE SPECIFIC DETERMINATION OF DNA SEQUENCES USING PARALLEL AMPLIFICATION
RU2213974C1 (en) * 2002-04-09 2003-10-10 Баженов Алексей Иванович Method for simultaneous detection of several analytes
WO2004020667A1 (en) * 2002-09-02 2004-03-11 Pamgene B.V. Novel integrated microarray analysis
DE10259819B4 (en) * 2002-12-19 2006-07-13 Siemens Ag Method for PCR amplification and detection of nucleotide sequences
DE102005029810B4 (en) * 2005-06-27 2008-11-13 Siemens Ag Method for detecting nucleotide sequences, use of the method and test set
WO2008140494A2 (en) * 2006-11-22 2008-11-20 The Board Of Trustees Of Michigan State University High throughput screening using microarrays
AU2013203278B2 (en) * 2007-02-02 2015-02-26 Genera Biosystems Limited Generation of nucleic acid molecules
JP5735213B2 (en) * 2007-02-02 2015-06-17 ジェネラ バイオシステムズ リミテッド Preparation method of nucleic acid molecule
EP2137523A1 (en) 2007-04-04 2009-12-30 Network Biosystems, Inc. Plastic microfluidic separation and detection platforms
EP2113574A1 (en) 2008-04-28 2009-11-04 Biotype AG Substances and methods for a DNA based profiling assay
JP2012529908A (en) 2009-06-15 2012-11-29 ネットバイオ・インコーポレーテッド Improved method for quantification of forensic DNA
US9315860B2 (en) * 2009-10-26 2016-04-19 Genovoxx Gmbh Conjugates of nucleotides and method for the application thereof
CA2751947C (en) * 2010-09-29 2018-10-16 Econous Systems Inc. Surface-oriented antibody coating for the reduction of post-stent restenosis
EP2455485A1 (en) 2010-11-19 2012-05-23 Anagnostics Bioanalysis GmbH Method for detecting nucleic acids

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US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
GB8716279D0 (en) 1987-07-10 1987-08-19 Amersham Int Plc Performing nucleic acid reactions
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WO1994016107A1 (en) 1993-01-12 1994-07-21 Thomas Jefferson University DNA SEQUENCING WITH Bst POLYMERASE
US5599660A (en) 1993-01-19 1997-02-04 Pharmacia Biotech Inc. Method and preparation for sequential delivery of wax-embedded, inactivated biological and chemical reagents
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US6642000B1 (en) 1999-11-12 2003-11-04 University Of Chicago PCR amplification on microarrays of gel immobilized oligonucleotides
ATE316581T1 (en) 2000-09-05 2006-02-15 Zeltz Patrick Dr METHOD FOR THE SPECIFIC DETERMINATION OF DNA SEQUENCES USING PARALLEL AMPLIFICATION
US6350580B1 (en) * 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure

Also Published As

Publication number Publication date
DE50012114D1 (en) 2006-04-13
ATE316581T1 (en) 2006-02-15
WO2002020833A2 (en) 2002-03-14
JP2004508053A (en) 2004-03-18
US20040048270A1 (en) 2004-03-11
US7611871B2 (en) 2009-11-03
EP1186669B1 (en) 2006-01-25
WO2002020833A3 (en) 2002-08-08
EP1186669A1 (en) 2002-03-13

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