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AU2001285735A1 - Synthetic spider silk proteins and the expression thereof in transgenic plants - Google Patents

Synthetic spider silk proteins and the expression thereof in transgenic plants

Info

Publication number
AU2001285735A1
AU2001285735A1 AU2001285735A AU8573501A AU2001285735A1 AU 2001285735 A1 AU2001285735 A1 AU 2001285735A1 AU 2001285735 A AU2001285735 A AU 2001285735A AU 8573501 A AU8573501 A AU 8573501A AU 2001285735 A1 AU2001285735 A1 AU 2001285735A1
Authority
AU
Australia
Prior art keywords
expression
transgenic plants
spider silk
silk proteins
synthetic spider
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001285735A
Inventor
Udo Conrad
Frank Grosse
Karl-Heinz Guehrs
Jurgen Scheller
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut fuer Pflanzengenetik und Kulturpflanzenforschung
Original Assignee
Institut fuer Pflanzengenetik und Kulturpflanzenforschung
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE10113781A external-priority patent/DE10113781A1/en
Application filed by Institut fuer Pflanzengenetik und Kulturpflanzenforschung filed Critical Institut fuer Pflanzengenetik und Kulturpflanzenforschung
Publication of AU2001285735A1 publication Critical patent/AU2001285735A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
    • C07K14/43586—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from silkworms

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Insects & Arthropods (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2001285735A 2000-06-09 2001-06-11 Synthetic spider silk proteins and the expression thereof in transgenic plants Abandoned AU2001285735A1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
DE10028212 2000-06-09
DE10028212 2000-06-09
DE10053478 2000-10-24
DE10053478 2000-10-24
DE10113781A DE10113781A1 (en) 2000-06-09 2001-03-21 New DNA encoding synthetic spider silk protein, useful e.g. for closing wounds, comprises modules that encode repeating units of spirodoin proteins
DE10113781 2001-03-21
PCT/EP2001/006586 WO2001094393A2 (en) 2000-06-09 2001-06-11 Synthetic spider silk proteins and the expression thereof in transgenic plants

Publications (1)

Publication Number Publication Date
AU2001285735A1 true AU2001285735A1 (en) 2001-12-17

Family

ID=27213905

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001285735A Abandoned AU2001285735A1 (en) 2000-06-09 2001-06-11 Synthetic spider silk proteins and the expression thereof in transgenic plants

Country Status (6)

Country Link
US (1) US20060248615A1 (en)
EP (1) EP1287139B1 (en)
AR (1) AR030426A1 (en)
AU (1) AU2001285735A1 (en)
CA (1) CA2411600A1 (en)
WO (1) WO2001094393A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6608242B1 (en) * 2000-05-25 2003-08-19 E. I. Du Pont De Nemours And Company Production of silk-like proteins in plants
WO2003057727A1 (en) * 2002-01-11 2003-07-17 Nexia Biotechnologies, Inc. Methods of producing silk polypeptides and products thereof
US7057023B2 (en) 2002-01-11 2006-06-06 Nexia Biotechnologies Inc. Methods and apparatus for spinning spider silk protein
DE102007002222A1 (en) 2007-01-10 2008-07-17 Gustav Pirazzi & Comp. Kg Use of artificially produced spider silk
BRPI0701826B1 (en) 2007-03-16 2021-02-17 Embrapa - Empresa Brasileira De Pesquisa Agropecuária spider web proteins nephilengys cruentata, avicularia juruensis and parawixia bistriata isolated from Brazilian biodiversity
WO2008151405A1 (en) * 2007-06-15 2008-12-18 Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Expression of fusion proteins containing a single chain antibody fragment linked to elastin-like repeating units in transgenic plants
KR101317420B1 (en) * 2010-03-11 2013-10-10 한국과학기술원 High Molecular Weight Recombinant Silk or Silk-like Proteins and Micro or Nano-spider Silk or Silk-like Fibres Manufactured by Using the Same
CA2816584A1 (en) * 2010-11-01 2012-05-10 Peptimed, Inc. Compositions of a peptide-based system for cell-specific targeting
EP2518081B1 (en) 2011-04-28 2017-11-29 Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK) Method of producing and purifying polymeric proteins in transgenic plants
US20180271939A1 (en) * 2017-03-24 2018-09-27 Milton J. Silverman, JR. Genetic method to kill cancer cells by suffocation
US12391912B2 (en) * 2021-10-26 2025-08-19 Rensselaer Polytechnic Institute Systems and methods for increased production of recombinant biopolymers via genome engineering and downregulation of basal expression
CN116425848B (en) * 2023-04-11 2024-05-24 北京新诚中科技术有限公司 Recombinant chimeric spider silk protein, biological protein fiber, and preparation methods and applications thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5770697A (en) * 1986-11-04 1998-06-23 Protein Polymer Technologies, Inc. Peptides comprising repetitive units of amino acids and DNA sequences encoding the same
ATE253635T1 (en) * 1993-06-15 2003-11-15 Du Pont RECOMBINANT SPINNER SILK ANALOGUE
IL123398A0 (en) * 1995-08-22 1998-09-24 Agricola Tech Inc Cloning methods for high strength spider silk proteins
US6608242B1 (en) * 2000-05-25 2003-08-19 E. I. Du Pont De Nemours And Company Production of silk-like proteins in plants

Also Published As

Publication number Publication date
US20060248615A1 (en) 2006-11-02
EP1287139A2 (en) 2003-03-05
EP1287139B1 (en) 2010-08-25
WO2001094393A2 (en) 2001-12-13
CA2411600A1 (en) 2001-12-13
WO2001094393A3 (en) 2002-06-20
AR030426A1 (en) 2003-08-20

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