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AU2001283934A1 - Method for the examination of cells in a culture medium - Google Patents

Method for the examination of cells in a culture medium

Info

Publication number
AU2001283934A1
AU2001283934A1 AU2001283934A AU8393401A AU2001283934A1 AU 2001283934 A1 AU2001283934 A1 AU 2001283934A1 AU 2001283934 A AU2001283934 A AU 2001283934A AU 8393401 A AU8393401 A AU 8393401A AU 2001283934 A1 AU2001283934 A1 AU 2001283934A1
Authority
AU
Australia
Prior art keywords
cells
windows
examination
separation
culture medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001283934A
Inventor
Christoph. Bittner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
innovatis AG
Original Assignee
INNOVATIS GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by INNOVATIS GmbH filed Critical INNOVATIS GmbH
Publication of AU2001283934A1 publication Critical patent/AU2001283934A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1493—Particle size

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Sustainable Development (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Image Processing (AREA)
  • Microscoopes, Condenser (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

The invention relates to a method for the examination of cells ( 20, 38 ) in a culture medium, in particular for in-situ microscopy in a bio-reactor, whereby cells in a sample volume, the depth (d) of which is defined by windows ( 14, 16 ) in the direction of the optical axis of the microscope ( 18 ), are microscopically imaged and are automatically recorded and processed by means of an image processing system ( 30 ). Said method is characterized in that the depth (d) of the sample volume ( 12 ) is adjusted to the size of the cells ( 20, 38 ) by successively reducing the separation (d) of the windows while the image size (G) of the cells is simultaneously verified by the image processing system ( 30 ) such that a separation value (D) is determined at which the image size (G) of the cells begins to grow, thus corresponding to flattening caused by the contact pressure of the windows ( 14, 16 ), and that the separation (d) of the windows ( 14, 16 ) is set to said separation value (D) for the examination.
AU2001283934A 2000-07-10 2001-07-07 Method for the examination of cells in a culture medium Abandoned AU2001283934A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10033268A DE10033268C2 (en) 2000-07-10 2000-07-10 Method for examining cells in a culture fluid
DE10033268 2000-07-10
PCT/EP2001/007814 WO2002004924A1 (en) 2000-07-10 2001-07-07 Method for the examination of cells in a culture medium

Publications (1)

Publication Number Publication Date
AU2001283934A1 true AU2001283934A1 (en) 2002-01-21

Family

ID=7648266

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001283934A Abandoned AU2001283934A1 (en) 2000-07-10 2001-07-07 Method for the examination of cells in a culture medium

Country Status (8)

Country Link
US (1) US7094562B2 (en)
EP (1) EP1299707B1 (en)
JP (1) JP2004503223A (en)
CN (1) CN1187437C (en)
AT (1) ATE286247T1 (en)
AU (1) AU2001283934A1 (en)
DE (2) DE10033268C2 (en)
WO (1) WO2002004924A1 (en)

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CA2563002C (en) * 2004-04-07 2011-07-12 Wardlaw Partners Lp Disposable chamber for analyzing biologic fluids
US7731901B2 (en) * 2005-10-19 2010-06-08 Abbott Laboratories Apparatus and method for performing counts within a biologic fluid sample
US7738094B2 (en) 2007-01-26 2010-06-15 Becton, Dickinson And Company Method, system, and compositions for cell counting and analysis
CN101435764B (en) * 2007-11-12 2013-11-27 北京深迈瑞医疗电子技术研究院有限公司 Particle analyzer and particle analysis method
WO2009117683A2 (en) 2008-03-21 2009-09-24 Abbott Point Of Care Method and apparatus for analyzing individual cells or particulates using fluorescent quenching and/or bleaching
CN102016686B (en) 2008-03-21 2015-03-25 艾博特健康公司 Method and apparatus for determining focus position of an imaging device imaging a biological sample
ES2560098T3 (en) 2008-03-21 2016-02-17 Abbott Point Of Care, Inc. Method and apparatus for detecting and counting platelets individually and in aggregates
WO2009117664A2 (en) * 2008-03-21 2009-09-24 Abbott Point Of Care, Inc. Method and apparatus for determining red blood cell indices of a blood sample utilizing the intrinsic pigmentation of hemoglobin contained within the red blood cells
WO2009117652A1 (en) * 2008-03-21 2009-09-24 Abbott Point Of Care, Inc. Method and apparatus for determining the hematocrit of a blood sample utilizing the intrinsic pigmentation of hemoglobin contained within the red blood cells
CA2720028C (en) * 2008-04-02 2013-10-08 Abbott Point Of Care, Inc. Method for serologic agglutination and other immunoassays performed in a thin film fluid sample
ES2597927T3 (en) * 2008-04-09 2017-01-24 Abbott Point Of Care, Inc. Procedure to measure the area of a sample arranged inside an analysis chamber
US20090258371A1 (en) * 2008-04-09 2009-10-15 Abbott Point Of Care, Inc. Method of detecting very low levels of analyte within a thin film fluid sample contained in a thin thickness chamber
DE102009012227B4 (en) * 2009-03-07 2011-09-01 Juri Charlot Containers for holding nutrient solutions or culture media
US20100255605A1 (en) * 2009-04-02 2010-10-07 Abbott Point Of Care, Inc. Method and device for transferring biologic fluid samples
US20140152801A1 (en) 2009-10-28 2014-06-05 Alentic Microscience Inc. Detecting and Using Light Representative of a Sample
JP2013509618A (en) * 2009-10-28 2013-03-14 アレンティック マイクロサイエンス インコーポレイテッド Microscopic imaging
US8837803B2 (en) * 2009-12-31 2014-09-16 Abbott Point Of Care, Inc. Method and apparatus for determining mean cell volume of red blood cells
CA2785648C (en) * 2009-12-31 2015-08-11 Abbott Point Of Care, Inc. Method and apparatus for securing planar orientation of analysis chamber
US8472693B2 (en) 2010-03-18 2013-06-25 Abbott Point Of Care, Inc. Method for determining at least one hemoglobin related parameter of a whole blood sample
CN103917870B (en) 2011-11-16 2016-04-13 贝克顿·迪金森公司 Methods and systems for detecting an analyte in a sample
US10416686B2 (en) 2012-06-18 2019-09-17 Greenonyx Ltd Compact apparatus for continuous production of a product substance from a starter material grown in aquaculture conditions
EP2943788B1 (en) 2013-01-11 2018-08-08 Becton Dickinson and Company Low-cost point-of-care assay device
US9518920B2 (en) 2013-06-26 2016-12-13 Alentic Microscience Inc. Sample processing improvements for microscopy
US10502666B2 (en) 2013-02-06 2019-12-10 Alentic Microscience Inc. Sample processing improvements for quantitative microscopy
US9063080B2 (en) 2013-07-26 2015-06-23 Ecolab Usa Inc. Method of deposition monitoring
BR112016009958B1 (en) 2013-11-06 2021-08-03 Becton, Dickinson And Company MICROFLUIDIC DEVICE, METHOD, SYSTEM AND KIT
JP6518245B2 (en) 2013-11-13 2019-05-22 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ Optical imaging system and method using the same
US10039244B2 (en) 2014-03-04 2018-08-07 Greenonyx Ltd Systems and methods for cultivating and distributing aquatic organisms
US9347082B2 (en) * 2014-08-28 2016-05-24 Omnivision Technologies, Inc. Automated cell growth/migration detection system and associated methods
WO2016060793A1 (en) 2014-10-14 2016-04-21 Becton, Dickinson And Company Blood sample management using open cell foam
EP4338668A1 (en) 2014-10-14 2024-03-20 Becton, Dickinson and Company Blood sample management using open cell foam
CA2954658C (en) 2015-03-10 2019-06-11 Becton, Dickinson And Company Biological fluid micro-sample management device
US10578606B2 (en) 2015-09-01 2020-03-03 Becton, Dickinson And Company Depth filtration device for separating specimen phases
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US11557034B2 (en) 2017-06-13 2023-01-17 The Trustees Of Princeton University Fully automatic, template-free particle picking for electron microscopy
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US5188968A (en) * 1989-12-28 1993-02-23 Olympus Optical Co., Ltd. Method and reaction kit for agglutination detection
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Also Published As

Publication number Publication date
EP1299707B1 (en) 2004-12-29
ATE286247T1 (en) 2005-01-15
CN1187437C (en) 2005-02-02
DE10033268A1 (en) 2002-02-07
US20040048330A1 (en) 2004-03-11
JP2004503223A (en) 2004-02-05
WO2002004924A1 (en) 2002-01-17
US7094562B2 (en) 2006-08-22
CN1441899A (en) 2003-09-10
DE10033268C2 (en) 2002-08-08
EP1299707A1 (en) 2003-04-09
DE50104979D1 (en) 2005-02-03

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