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AU2001277014A1 - Modular vector systems - Google Patents

Modular vector systems

Info

Publication number
AU2001277014A1
AU2001277014A1 AU2001277014A AU7701401A AU2001277014A1 AU 2001277014 A1 AU2001277014 A1 AU 2001277014A1 AU 2001277014 A AU2001277014 A AU 2001277014A AU 7701401 A AU7701401 A AU 7701401A AU 2001277014 A1 AU2001277014 A1 AU 2001277014A1
Authority
AU
Australia
Prior art keywords
vector systems
modular vector
modular
systems
vector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001277014A
Inventor
William Donahue
Kevin A. Jarrell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boston University
Original Assignee
Boston University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boston University filed Critical Boston University
Publication of AU2001277014A1 publication Critical patent/AU2001277014A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2001277014A 2000-07-21 2001-07-20 Modular vector systems Abandoned AU2001277014A1 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
USNOTGIVEN 1997-05-02
US21982000P 2000-07-21 2000-07-21
US60/219,820 2000-07-21
PCT/US2001/022836 WO2002008408A2 (en) 2000-07-21 2001-07-20 Modular vector systems

Publications (1)

Publication Number Publication Date
AU2001277014A1 true AU2001277014A1 (en) 2002-02-05

Family

ID=22820912

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001277014A Abandoned AU2001277014A1 (en) 2000-07-21 2001-07-20 Modular vector systems

Country Status (3)

Country Link
US (1) US20030017552A1 (en)
AU (1) AU2001277014A1 (en)
WO (1) WO2002008408A2 (en)

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US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
CA2558749A1 (en) * 2004-02-27 2005-09-29 President And Fellows Of Harvard College Polynucleotide synthesis
AU2005295351A1 (en) * 2004-10-18 2006-04-27 Codon Devices, Inc. Methods for assembly of high fidelity synthetic polynucleotides
US20070122817A1 (en) * 2005-02-28 2007-05-31 George Church Methods for assembly of high fidelity synthetic polynucleotides
CA2594832A1 (en) * 2005-01-13 2006-07-20 Codon Devices, Inc. Compositions and methods for protein design
US20060281113A1 (en) * 2005-05-18 2006-12-14 George Church Accessible polynucleotide libraries and methods of use thereof
US20070004041A1 (en) * 2005-06-30 2007-01-04 Codon Devices, Inc. Heirarchical assembly methods for genome engineering
WO2007008951A1 (en) * 2005-07-12 2007-01-18 Codon Devices, Inc. Compositions and methods for design of non-immunogenic proteins
AU2006267039A1 (en) * 2005-07-12 2007-01-18 Codon Devices, Inc. Compositions and methods for biocatalytic engineering
WO2007136834A2 (en) * 2006-05-19 2007-11-29 Codon Devices, Inc. Combined extension and ligation for nucleic acid assembly
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
CN103320506B (en) * 2007-07-03 2015-10-28 吉纳珀莱有限公司 For improvement of the chimeric primers of nucleic acid amplification reaction
WO2011056872A2 (en) 2009-11-03 2011-05-12 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
EP2637780B1 (en) 2010-11-12 2022-02-09 Gen9, Inc. Protein arrays and methods of using and making the same
EP3360963B1 (en) 2010-11-12 2019-11-06 Gen9, Inc. Methods and devices for nucleic acids synthesis
LT3594340T (en) 2011-08-26 2021-10-25 Gen9, Inc. Compositions and methods for high fidelity assembly of nucleic acids
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
EP2841601B1 (en) 2012-04-24 2019-03-06 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
EP3483311B8 (en) 2012-06-25 2025-10-29 Ginkgo Bioworks, Inc. Methods for nucleic acid assembly and high throughput sequencing
WO2014088693A1 (en) * 2012-12-06 2014-06-12 Agilent Technologies, Inc. Molecular fabrication
GB201516348D0 (en) * 2015-09-15 2015-10-28 Labgenius Ltd Compositions and methods for polynucleotide assembly
CN114901834A (en) * 2019-12-30 2022-08-12 安捷伦科技有限公司 Chimeric primers and related methods

Family Cites Families (33)

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EP1186660A3 (en) * 1985-03-30 2002-03-20 KAUFFMAN, Stuart A. Method for obtaining DNA, RNA, peptides, polypeptides or proteins by means of a DNA-recombination technique
US5128256A (en) * 1987-01-12 1992-07-07 Stratagene DNA cloning vectors with in vivo excisable plasmids
WO1990004788A1 (en) * 1988-10-28 1990-05-03 Genentech, Inc. Method for identifying active domains and amino acid residues in polypeptides and hormone variants
US6780613B1 (en) * 1988-10-28 2004-08-24 Genentech, Inc. Growth hormone variants
US5300431A (en) * 1991-02-26 1994-04-05 E. I. Du Pont De Nemours And Company Positive selection vector for the bacteriophage P1 cloning system
US6150141A (en) * 1993-09-10 2000-11-21 Trustees Of Boston University Intron-mediated recombinant techniques and reagents
US5498531A (en) * 1993-09-10 1996-03-12 President And Fellows Of Harvard College Intron-mediated recombinant techniques and reagents
US6995017B1 (en) * 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6335160B1 (en) * 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5710000A (en) * 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
US6939689B2 (en) * 1995-12-07 2005-09-06 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US7018793B1 (en) * 1995-12-07 2006-03-28 Diversa Corporation Combinatorial screening of mixed populations of organisms
US6171820B1 (en) * 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US6972183B1 (en) * 1997-06-16 2005-12-06 Diversa Corporation Capillary array-based enzyme screening
US6537776B1 (en) * 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6280978B1 (en) * 1995-12-15 2001-08-28 Intronn Holdings, Llc Methods and compositions for use in spliceosome mediated RNA trans-splicing
US6261797B1 (en) * 1996-01-29 2001-07-17 Stratagene Primer-mediated polynucleotide synthesis and manipulation techniques
US6825011B1 (en) * 1998-12-17 2004-11-30 Yuri Rumantichikov Methods for insertion of nucleic acids into circular vectors
US6277632B1 (en) * 1996-06-17 2001-08-21 Vectorobjects, Llc Method and kits for preparing multicomponent nucleic acid constructs
US6495318B2 (en) * 1996-06-17 2002-12-17 Vectorobjects, Llc Method and kits for preparing multicomponent nucleic acid constructs
US7148054B2 (en) * 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
AU6443298A (en) * 1997-02-28 1998-09-18 Nature Technology Corporation Self-assembling genes, vectors and uses thereof
US6297007B1 (en) * 1997-05-22 2001-10-02 Terragen Diversity Inc. Method for isolation of biosynthesis genes for bioactive molecules
JP2002537762A (en) * 1999-01-05 2002-11-12 トラスティーズ オブ ボストン ユニバーシティ Improved nucleic acid cloning
US20040005673A1 (en) * 2001-06-29 2004-01-08 Kevin Jarrell System for manipulating nucleic acids
US20070065838A1 (en) * 1999-01-19 2007-03-22 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US7024312B1 (en) * 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
ES2604329T3 (en) * 2001-02-21 2017-03-06 Basf Enzymes Llc Enzymes that have alpha amylase activity and methods of using them
US6979733B2 (en) * 2001-08-03 2005-12-27 Diversa Corporation Epoxide hydrolases, nucleic acids encoding them and methods of making and using them
US7314613B2 (en) * 2002-11-18 2008-01-01 Maxygen, Inc. Interferon-alpha polypeptides and conjugates
EP1608766A4 (en) * 2003-03-20 2006-11-02 Diversa Corp GLUCOSIDASES, NUCLEIC ACIDS ENCODING THEM, AND METHODS OF MAKING AND USING THE SAME
RU2006145020A (en) * 2004-05-19 2008-06-27 Максиджен, Инк. (Us) Interferon-alpha polypeptides and conjugates
US20070292954A1 (en) * 2006-04-21 2007-12-20 The Brigham And Women's Hospital, Inc. Generation of recombinant DNA by sequence-and ligation-independent cloning

Also Published As

Publication number Publication date
WO2002008408A2 (en) 2002-01-31
WO2002008408A3 (en) 2002-08-29
US20030017552A1 (en) 2003-01-23

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