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AU2001275649A1 - Method for the high-parallel analysis of polymorphisms - Google Patents

Method for the high-parallel analysis of polymorphisms

Info

Publication number
AU2001275649A1
AU2001275649A1 AU2001275649A AU7564901A AU2001275649A1 AU 2001275649 A1 AU2001275649 A1 AU 2001275649A1 AU 2001275649 A AU2001275649 A AU 2001275649A AU 7564901 A AU7564901 A AU 7564901A AU 2001275649 A1 AU2001275649 A1 AU 2001275649A1
Authority
AU
Australia
Prior art keywords
allele
probes
polymorphisms
nucleic acid
specific
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001275649A
Inventor
Kurt Berlin
Ivo Glynne Gut
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Epigenomics AG
Original Assignee
Epigenomics AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics AG filed Critical Epigenomics AG
Publication of AU2001275649A1 publication Critical patent/AU2001275649A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)

Abstract

Disclosed is a method for the high-parallel characterisation of polymorphisms, especially SNPs. Said method can also be used for the simultaneous or separate detection of DNA methylation. First, a set of probes provided with at least one characteristic detectable identifying mark for a respective probe is connected to an addressed surface. A nucleic acid to be examined is then hybridised to these probes and the probes are extended in an allele-specific enzymatic reaction. The type and the occurrence of said allele-specific reaction determine whether the respective probe is enzymatically decomposed. Finally, the remaining allele-specific products are analysed and the existing alleles in the extracted nucleic acid samples are determined.
AU2001275649A 2000-06-19 2001-06-19 Method for the high-parallel analysis of polymorphisms Abandoned AU2001275649A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10029914A DE10029914A1 (en) 2000-06-19 2000-06-19 Method for the highly parallel analysis of polymorphisms
DE10029914 2000-06-19
PCT/DE2001/002273 WO2001098527A2 (en) 2000-06-19 2001-06-19 Method for the high-parallel analysis of polymorphisms

Publications (1)

Publication Number Publication Date
AU2001275649A1 true AU2001275649A1 (en) 2002-01-02

Family

ID=7646112

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001275649A Abandoned AU2001275649A1 (en) 2000-06-19 2001-06-19 Method for the high-parallel analysis of polymorphisms

Country Status (7)

Country Link
US (1) US20030104464A1 (en)
EP (1) EP1292711B1 (en)
AT (1) ATE292193T1 (en)
AU (1) AU2001275649A1 (en)
CA (1) CA2411400A1 (en)
DE (2) DE10029914A1 (en)
WO (1) WO2001098527A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10104938B4 (en) * 2001-01-29 2005-06-23 Epigenomics Ag Fluorescence Polarization 1
KR20040036900A (en) 2001-07-15 2004-05-03 켁크 그래주에이트 인스티튜트 Amplification of nucleic acid fragments using nicking agents
US20110151438A9 (en) * 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
DE10240746A1 (en) * 2002-09-01 2004-03-18 Epigenomics Ag Method for the detection of nucleic acid sequences using cleavable probe molecules
US20040101843A1 (en) * 2002-11-22 2004-05-27 Gerald Zon Detection of methylated DNA sites
CA2519456A1 (en) * 2003-03-17 2004-09-30 Michael G. Goggins Aberrantly methylated genes in pancreatic cancer
US20050069895A1 (en) * 2003-08-29 2005-03-31 Applera Corporation Compositions, methods, and kits for fabricating coded molecular tags
US20050048498A1 (en) * 2003-08-29 2005-03-03 Applera Corporation Compositions, methods, and kits for assembling probes
US7198900B2 (en) * 2003-08-29 2007-04-03 Applera Corporation Multiplex detection compositions, methods, and kits
US20060292585A1 (en) * 2005-06-24 2006-12-28 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
US7901882B2 (en) 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
JP2010527241A (en) * 2007-05-14 2010-08-12 インサイト ジェネティクス， インコーポレイテッド Methods for screening nucleic acids for single nucleotide variations
GB2587939B (en) 2018-04-02 2023-06-14 Grail Llc Methylation markers and targeted methylation probe panels
CA3111887A1 (en) 2018-09-27 2020-04-02 Grail, Inc. Methylation markers and targeted methylation probe panel

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4521509A (en) * 1982-11-24 1985-06-04 Research Corporation Method for degrading DNA
US5518900A (en) * 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
US6468742B2 (en) * 1993-11-01 2002-10-22 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using bioelectronic microchip
ATE247716T1 (en) * 1994-02-07 2003-09-15 Beckman Coulter Inc LIGASE/POLYMERASE-MEDIATED ANALYSIS OF GENETIC ELEMENTS OF SINGLE NUCLEOTIDE POLYMORPHISMS AND THEIR USE IN GENETIC ANALYSIS
DE19530336C2 (en) * 1995-08-17 1997-08-28 Univ Eberhard Karls Sequential hybridization of fungal cell DNA and methods for detecting and identifying fungal cells in clinical material
US5919626A (en) * 1997-06-06 1999-07-06 Orchid Bio Computer, Inc. Attachment of unmodified nucleic acids to silanized solid phase surfaces
DE19754482A1 (en) * 1997-11-27 1999-07-01 Epigenomics Gmbh Process for making complex DNA methylation fingerprints
US6723564B2 (en) * 1998-05-07 2004-04-20 Sequenom, Inc. IR MALDI mass spectrometry of nucleic acids using liquid matrices
DE69936379T2 (en) * 1998-09-25 2008-02-28 Massachusetts Institute Of Technology, Cambridge METHOD FOR GENOTYPIZING AND DNA ANALYSIS
JP2003522527A (en) * 1999-12-28 2003-07-29 グラクソ グループ リミテッド Single nucleotide polymorphism detection
DE10021204A1 (en) * 2000-04-25 2001-11-08 Epigenomics Ag Method for the highly parallel analysis of polymorphisms
EP1164201A1 (en) * 2000-06-14 2001-12-19 Facultés Universitaires Notre-Dame de la Paix Reverse detection for identification and/or quantification of nucleotide target sequences on biochips

Also Published As

Publication number Publication date
ATE292193T1 (en) 2005-04-15
DE50105784D1 (en) 2005-05-04
CA2411400A1 (en) 2002-12-06
EP1292711B1 (en) 2005-03-30
US20030104464A1 (en) 2003-06-05
WO2001098527A2 (en) 2001-12-27
WO2001098527A3 (en) 2002-06-27
DE10029914A1 (en) 2002-01-03
EP1292711A2 (en) 2003-03-19

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Legal Events

Date Code Title Description
MK5 Application lapsed section 142(2)(e) - patent request and compl. specification not accepted