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AU2001271560A1 - Method for transformation of agaricus bisporus - Google Patents

Method for transformation of agaricus bisporus

Info

Publication number
AU2001271560A1
AU2001271560A1 AU2001271560A AU7156001A AU2001271560A1 AU 2001271560 A1 AU2001271560 A1 AU 2001271560A1 AU 2001271560 A AU2001271560 A AU 2001271560A AU 7156001 A AU7156001 A AU 7156001A AU 2001271560 A1 AU2001271560 A1 AU 2001271560A1
Authority
AU
Australia
Prior art keywords
transformation
agaricus bisporus
protocol
preferred
expeditious
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001271560A
Inventor
Xi Chen
C. Peter Romaine
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Penn State Research Foundation
Original Assignee
Penn State Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Penn State Research Foundation filed Critical Penn State Research Foundation
Publication of AU2001271560A1 publication Critical patent/AU2001271560A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Mushroom Cultivation (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Applicants have devised a highly effective, convenient, and expeditious genetic transformation system for filamentous fungi, such as Agaricus bisporus. The preferred method uses an Agrobacterium-mediated transformation protocol. The critical features of this protocol include co-cultivation of the bacterium with fruit body tissue instead of spores. In a preferred embodiment, even higher transformation efficiencies were observed with the use of a homologous promoter in the polynucleotide expression construct in order to drive gene expression.
AU2001271560A 2000-06-28 2001-06-28 Method for transformation of agaricus bisporus Abandoned AU2001271560A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US21463000P 2000-06-28 2000-06-28
US60/214,630 2000-06-28
US09/894,630 US6964866B2 (en) 2000-06-28 2001-06-28 Methods and compositions for highly efficient transformation of filamentous fungi
PCT/US2001/020566 WO2002000896A2 (en) 2000-06-28 2001-06-28 Method for transformation of agaricus bisporus
US09/894,630 2001-06-28

Publications (1)

Publication Number Publication Date
AU2001271560A1 true AU2001271560A1 (en) 2002-01-08

Family

ID=26909200

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001271560A Abandoned AU2001271560A1 (en) 2000-06-28 2001-06-28 Method for transformation of agaricus bisporus

Country Status (8)

Country Link
US (2) US6964866B2 (en)
EP (1) EP1409693B1 (en)
AT (1) ATE390488T1 (en)
AU (1) AU2001271560A1 (en)
CA (1) CA2452183C (en)
DE (1) DE60133422T2 (en)
ES (1) ES2302739T3 (en)
WO (1) WO2002000896A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8524220B1 (en) 2010-02-09 2013-09-03 David Gordon Bermudes Protease inhibitor: protease sensitivity expression system composition and methods improving the therapeutic activity and specificity of proteins delivered by bacteria
US9597379B1 (en) 2010-02-09 2017-03-21 David Gordon Bermudes Protease inhibitor combination with therapeutic proteins including antibodies
US8771669B1 (en) 2010-02-09 2014-07-08 David Gordon Bermudes Immunization and/or treatment of parasites and infectious agents by live bacteria
US8686218B2 (en) 2010-04-14 2014-04-01 The Penn State Research Foundation Strategies for the transgenic manipulation of filamentous fungi
CA2851855A1 (en) * 2011-10-31 2013-05-10 Bp Corporation North America Inc. Use of plant promoters in filamentous fungi
US9737592B1 (en) 2014-02-14 2017-08-22 David Gordon Bermudes Topical and orally administered protease inhibitors and bacterial vectors for the treatment of disorders and methods of treatment
CN104726344B (en) * 2014-08-25 2019-10-22 上海市农业科学院 A strain Hu F2 suitable for industrial cultivation of shiitake mushrooms and its fingerprint and cultivation method
CN104651389B (en) * 2014-12-29 2017-06-06 河南农业大学 A kind of method of liposome-mediated transfection agaricus bisporus lamella
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
CN106244620A (en) * 2016-09-09 2016-12-21 福州大学 It is independent of medium and directly converts the method that foreign DNA enters aspergillus niger resting spore
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
CN107916273A (en) * 2017-10-23 2018-04-17 上海市农业科学院 A kind of method of agaricus bisporus genetic transformation
CN109628626B (en) * 2018-12-07 2022-07-26 东莞东阳光保健品研发有限公司 Specific primer, kit and method for identifying morchella ladder and application of specific primer, kit and method
US12285437B2 (en) 2019-10-30 2025-04-29 The Research Foundation For The State University Of New York Reversing the undesirable pH-profile of doxorubicin via activation of a disubstituted maleamic acid prodrug at tumor acidity

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0708826A1 (en) 1993-07-13 1996-05-01 Bromyc B.V. Production and application of transgenic mushroom mycelium and fruitbodies
US6255115B1 (en) * 1997-04-07 2001-07-03 Unilever Patent Holdings Bv Agrobacterium mediated transformation of moulds, in particular those belonging to the genus Aspergillus

Also Published As

Publication number Publication date
US6964866B2 (en) 2005-11-15
EP1409693B1 (en) 2008-03-26
WO2002000896A2 (en) 2002-01-03
US20050070007A1 (en) 2005-03-31
WO2002000896A3 (en) 2003-08-28
CA2452183C (en) 2013-12-24
DE60133422T2 (en) 2008-08-21
CA2452183A1 (en) 2002-01-03
US7700349B2 (en) 2010-04-20
EP1409693A2 (en) 2004-04-21
US20020016982A1 (en) 2002-02-07
ES2302739T3 (en) 2008-08-01
DE60133422D1 (en) 2008-05-08
ATE390488T1 (en) 2008-04-15

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