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AU2001269757A1 - Large-scale recombinant adeno-associated virus (raav) production and purification - Google Patents

Large-scale recombinant adeno-associated virus (raav) production and purification

Info

Publication number
AU2001269757A1
AU2001269757A1 AU2001269757A AU6975701A AU2001269757A1 AU 2001269757 A1 AU2001269757 A1 AU 2001269757A1 AU 2001269757 A AU2001269757 A AU 2001269757A AU 6975701 A AU6975701 A AU 6975701A AU 2001269757 A1 AU2001269757 A1 AU 2001269757A1
Authority
AU
Australia
Prior art keywords
raav
purification
production
associated virus
recombinant adeno
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001269757A
Inventor
Guang Qu
John Fraser Wright
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Avigen Inc
Original Assignee
Avigen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Avigen Inc filed Critical Avigen Inc
Publication of AU2001269757A1 publication Critical patent/AU2001269757A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011Details
    • C12N2750/14011Parvoviridae
    • C12N2750/14111Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151Methods of production or purification of viral material

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2001269757A 2000-08-07 2001-06-07 Large-scale recombinant adeno-associated virus (raav) production and purification Abandoned AU2001269757A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09633834 2000-08-07
US09/633,834 US6593123B1 (en) 2000-08-07 2000-08-07 Large-scale recombinant adeno-associated virus (rAAV) production and purification
PCT/US2001/018443 WO2002012455A1 (en) 2000-08-07 2001-06-07 LARGE-SCALE RECOMBINANT ADENO-ASSOCIATED VIRUS (rAAV) PRODUCTION AND PURIFICATION

Publications (1)

Publication Number Publication Date
AU2001269757A1 true AU2001269757A1 (en) 2002-02-18

Family

ID=24541304

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001269757A Abandoned AU2001269757A1 (en) 2000-08-07 2001-06-07 Large-scale recombinant adeno-associated virus (raav) production and purification

Country Status (3)

Country Link
US (2) US6593123B1 (en)
AU (1) AU2001269757A1 (en)
WO (1) WO2002012455A1 (en)

Families Citing this family (109)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070122904A1 (en) * 2000-09-29 2007-05-31 Unisearch Limited Method and apparatus for culturing cells
US20030175688A1 (en) * 2002-03-15 2003-09-18 Rukmini Pennathur-Das Method for the purification and production of oncolytic adenoviruses
US20050009168A1 (en) * 2003-05-12 2005-01-13 Robbins Joan Marie Methods and apparatus for Adeno associated virus purification
DE602004030327D1 (en) 2003-05-21 2011-01-13 Genzyme Corp METHOD FOR PRODUCING PREPARATIONS OF RECOMBINANT AAV VIRIONS LARGELY FREE FROM EMPTY CAPSIDES
US20040248826A1 (en) * 2003-06-03 2004-12-09 Alberto Auricchio Treatment of cancer by in vivo gene-transfer induced TIMP-3 expression
US7261882B2 (en) 2003-06-23 2007-08-28 Reagents Of The University Of Colorado Methods for treating neuropathic pain by administering IL-10 polypeptides
MX360727B (en) 2004-06-01 2018-11-14 Genzyme Corp Compositions and methods to prevent aav vector aggregation.
AU2005322353B2 (en) 2004-12-23 2011-09-01 Medimmune, Llc Non-tumorigenic MDCK cell line for propagating viruses
KR100823157B1 (en) * 2004-12-30 2008-04-21 재단법인 목암생명공학연구소 How to mass produce recombinant adeno-associated viruses
US9198984B2 (en) 2006-04-28 2015-12-01 The Trustees Of The University Of Pennsylvania Scalable production method for AAV
CA2663522C (en) 2006-09-15 2015-06-02 Medimmune, Llc Mdck cell lines supporting viral growth to high titers and bioreactor process using the same
EP2031048B2 (en) 2007-08-31 2019-05-01 The Procter and Gamble Company Liquid acidic hard surface cleaning composition
EP2039747A1 (en) 2007-09-17 2009-03-25 The Procter and Gamble Company Process for treating hard surface
EP2039748A1 (en) 2007-09-17 2009-03-25 The Procter and Gamble Company Process of treating inclined hard surface
CN102216450B (en) 2008-09-24 2014-05-14 米迪缪尼有限公司 Methods of Culturing Cells, Propagating and Purifying Viruses
HUE057606T2 (en) 2009-05-02 2022-05-28 Genzyme Corp Gene therapy for neurodegenerative disorders
IN2012DN06629A (en) * 2010-01-28 2015-10-23 Philadelphia Children Hospital
WO2011097447A2 (en) * 2010-02-04 2011-08-11 Neurologix, Inc. Production of recombinant virus
WO2012048275A2 (en) 2010-10-08 2012-04-12 Caridianbct, Inc. Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
EP3219313B1 (en) 2011-04-18 2020-10-28 National Center of Neurology and Psychiatry Drug delivery particle and method for producing the same
US20140107321A1 (en) * 2011-05-26 2014-04-17 Dr. Reddy's Laboratories Limited Purification of antibodies
SI2744895T1 (en) * 2011-09-08 2016-03-31 Uniqure Ip B.V. Removal of contaminating viruses from aav preparations
HUE037482T2 (en) 2013-01-08 2018-08-28 Genzyme Corp Use of Inos inhibitors to increase virus yield in culture
US11332719B2 (en) 2013-03-15 2022-05-17 The Broad Institute, Inc. Recombinant virus and preparations thereof
SG11201508297YA (en) 2013-04-17 2015-11-27 Genzyme Corp Compositions and methods for treating and preventing macular degeneration
KR102069561B1 (en) 2013-07-11 2020-01-23 다카라 바이오 가부시키가이샤 Method for manufacturing non-enveloped virus
KR102234695B1 (en) 2014-02-06 2021-04-02 젠자임 코포레이션 Compositions and methods for treating and preventing macular degeneration
WO2015191508A1 (en) 2014-06-09 2015-12-17 Voyager Therapeutics, Inc. Chimeric capsids
US10023846B2 (en) 2014-07-10 2018-07-17 Takara Bio Inc. Production method for non-enveloped virus particles
WO2016049421A1 (en) 2014-09-26 2016-03-31 Terumo Bct, Inc. Scheduled feed
MX2017005834A (en) 2014-11-05 2017-11-17 Voyager Therapeutics Inc Aadc polynucleotides for the treatment of parkinson's disease.
SG10202001102XA (en) 2014-11-14 2020-03-30 Voyager Therapeutics Inc Modulatory polynucleotides
SG11201703281RA (en) 2014-11-14 2017-05-30 Voyager Therapeutics Inc Compositions and methods of treating amyotrophic lateral sclerosis (als)
EP3230441A4 (en) 2014-12-12 2018-10-03 Voyager Therapeutics, Inc. Compositions and methods for the production of scaav
EP3243904A4 (en) 2015-01-09 2018-06-27 Takara Bio Inc. Method for producing non-enveloped viral particles
CN107428799A (en) 2015-01-13 2017-12-01 阿尔法韦士曼公司 Purify adeno-associated virus (AAV) and/or the method and its gradient and flow type buffer solution of recombinant adeno-associated virus (rAAV)
EP3054006A1 (en) 2015-02-09 2016-08-10 Institut National De La Sante Et De La Recherche Medicale (Inserm) Recombinant adeno-associated virus particle purification with multiple-step anion exchange chromatography
EP3054007A1 (en) * 2015-02-09 2016-08-10 Institut National De La Sante Et De La Recherche Medicale (Inserm) Recombinant adeno-associated virus particle purification comprising an immuno-affinity purification step
EP3368054A4 (en) 2015-10-28 2019-07-03 Voyager Therapeutics, Inc. REGULATORY EXPRESSION USING AN ADENO-ASSOCIATED VIRUS (AAV)
PH12018501168B1 (en) * 2015-12-01 2024-05-17 Spark Therapeutics Inc Scalable methods for producing recombinant adeno-associated viral (aav) vector in serum-free suspension cell culture system suitable for clinical use
WO2017160360A2 (en) 2015-12-11 2017-09-21 The Trustees Of The University Of Pennsylvania Scalable purification method for aav9
EP3992283A1 (en) 2015-12-11 2022-05-04 The Trustees Of The University Of Pennsylvania Scalable purification method for aavrh10
WO2017100674A1 (en) 2015-12-11 2017-06-15 The Trustees Of The University Of Pennsylvania Scalable purification method for aav1
EP3387117B1 (en) 2015-12-11 2022-11-23 The Trustees Of The University Of Pennsylvania Scalable purification method for aav8
WO2017112948A1 (en) * 2015-12-24 2017-06-29 University Of Florida Research Foundation, Inc. Improved aav production using suspension adapted cells
JP7364306B2 (en) 2016-03-31 2023-10-18 スパーク セラピューティクス インコーポレイテッド Column-based highly scalable rAAV manufacturing process
EP3448874A4 (en) 2016-04-29 2020-04-22 Voyager Therapeutics, Inc. Compositions for the treatment of disease
EP3448987A4 (en) 2016-04-29 2020-05-27 Voyager Therapeutics, Inc. Compositions for the treatment of disease
WO2017201258A1 (en) 2016-05-18 2017-11-23 Voyager Therapeutics, Inc. Compositions and methods of treating huntington's disease
JP7066635B2 (en) 2016-05-18 2022-05-13 ボイジャー セラピューティクス インコーポレイテッド Modulatory polynucleotide
CN109415696A (en) 2016-05-25 2019-03-01 泰尔茂比司特公司 Cell amplification
EP4219724A3 (en) 2016-08-23 2023-09-27 Akouos, Inc. Compositions and methods for treating non-age-associated hearing impairment in a human subject
US11298041B2 (en) 2016-08-30 2022-04-12 The Regents Of The University Of California Methods for biomedical targeting and delivery and devices and systems for practicing the same
CN110325203A (en) 2017-01-07 2019-10-11 西莱克塔生物科技公司 Medelling with the immunosuppressor of synthesis nano-carrier coupling is administered
CN110612344B (en) 2017-03-31 2023-09-12 泰尔茂比司特公司 cell expansion
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
CA3061652A1 (en) 2017-05-05 2018-11-08 Voyager Therapeutics, Inc. Compositions and methods of treating amyotrophic lateral sclerosis (als)
AU2018261003A1 (en) 2017-05-05 2019-11-14 Voyager Therapeutics, Inc. Compositions and methods of treating Huntington's Disease
CA3066358A1 (en) 2017-06-07 2018-12-13 Spark Therapeutics, Inc. Enhancing agents for improved cell transfection and/or raav vector production
JOP20190269A1 (en) 2017-06-15 2019-11-20 Voyager Therapeutics Inc Aadc polynucleotides for the treatment of parkinson's disease
PE20200737A1 (en) * 2017-06-30 2020-07-23 Spark Therapeutics Inc AAV VECTOR COLUMN PURIFICATION METHODS
US11497576B2 (en) 2017-07-17 2022-11-15 Voyager Therapeutics, Inc. Trajectory array guide system
US11512327B2 (en) 2017-08-03 2022-11-29 Voyager Therapeutics, Inc. Compositions and methods for delivery of AAV
AU2018338728B2 (en) 2017-09-29 2025-01-02 Centre National De La Recherche Scientifique (Cnrs) Rescue of central and peripheral neurological phenotype of Friedreich's Ataxia by intravenous delivery
WO2019079242A1 (en) 2017-10-16 2019-04-25 Voyager Therapeutics, Inc. Treatment of amyotrophic lateral sclerosis (als)
TW202413649A (en) 2017-10-16 2024-04-01 美商航海家醫療公司 Treatment of amyotrophic lateral sclerosis (als)
SG11202006232SA (en) * 2017-12-29 2020-07-29 Baxalta Inc Adeno-associated virus purification methods
EP3762500A1 (en) 2018-03-06 2021-01-13 Voyager Therapeutics, Inc. Insect cell manufactured partial self-complementary aav genomes
US12319929B2 (en) 2018-05-15 2025-06-03 Voyager Therapeutics, Inc. Compositions and methods for the treatment of Parkinson's disease
US20210207167A1 (en) 2018-05-16 2021-07-08 Voyager Therapeutics, Inc. Aav serotypes for brain specific payload delivery
EP3793615A2 (en) 2018-05-16 2021-03-24 Voyager Therapeutics, Inc. Directed evolution of aav to improve tropism for cns
AU2019285186B2 (en) 2018-06-14 2025-05-29 Regenxbio Inc. Anion exchange chromatography for recombinant AAV production
CN112567035A (en) 2018-07-02 2021-03-26 沃雅戈治疗公司 Treatment of amyotrophic lateral sclerosis and spinal cord related disorders
EP3826719A1 (en) 2018-07-24 2021-06-02 Voyager Therapeutics, Inc. Systems and methods for producing gene therapy formulations
CN109182281B (en) * 2018-08-31 2022-08-02 首都医科大学 Method and kit for treating adeno-associated virus
CN121160803A (en) 2018-09-28 2025-12-19 沃雅戈治疗公司 Ataxin expression constructs with engineered promoters and methods of use thereof
US20210348242A1 (en) 2018-10-04 2021-11-11 Voyager Therapeutics, Inc. Methods for measuring the titer and potency of viral vector particles
US20220064671A1 (en) 2019-01-18 2022-03-03 Voyager Therapeutics, Inc. Methods and systems for producing aav particles
CA3138525A1 (en) 2019-04-28 2020-11-05 Selecta Biosciences, Inc. Methods for treatment of subjects with preexisting immunity to viral transfer vectors
WO2020223274A1 (en) 2019-04-29 2020-11-05 Voyager Therapeutics, Inc. SYSTEMS AND METHODS FOR PRODUCING BACULOVIRAL INFECTED INSECT CELLS (BIICs) IN BIOREACTORS
JP7539420B2 (en) * 2019-06-28 2024-08-23 武田薬品工業株式会社 Methods for purifying adeno-associated viruses
EP4010465A1 (en) 2019-08-09 2022-06-15 Voyager Therapeutics, Inc. Cell culture medium for use in producing gene therapy products in bioreactors
TW202122582A (en) 2019-08-26 2021-06-16 美商航海家醫療公司 Controlled expression of viral proteins
WO2021124152A1 (en) 2019-12-19 2021-06-24 Pfizer Inc. Compositions and methods for nucleic acid transfection using cationic polymers and stabilizers
MX2022010050A (en) 2020-02-21 2022-09-05 Akouos Inc Compositions and methods for treating non-age-associated hearing impairment in a human subject.
EP3919613B1 (en) * 2020-06-05 2024-08-07 Sartorius BIA Separations d.o.o. Enhanced purification of adeno-associated virus to more effectively remove contaminating dna
US20230295656A1 (en) 2020-08-06 2023-09-21 Voyager Therapeutics, Inc. Cell culture medium for use in producing gene therapy products in bioreactors
WO2022043926A1 (en) * 2020-08-31 2022-03-03 Intas Pharmaceuticals Ltd. Process for preparation of recombinant adeno-associated virus particle
EP4217087A2 (en) * 2020-09-23 2023-08-02 Codiak BioSciences, Inc. Process for preparing extracellular vesicles
AR123776A1 (en) 2020-10-15 2023-01-11 Hoffmann La Roche NUCLEIC ACID CONSTRUCTS FOR THE SIMULTANEOUS ACTIVATION OF GENES
CA3197730A1 (en) 2020-10-15 2022-04-21 F. Hoffman-La Roche Ag Nucleic acid constructs for va rna transcription
JP7683230B2 (en) * 2021-01-27 2025-05-27 東ソー株式会社 Methods for purifying adeno-associated viruses
WO2022187548A1 (en) 2021-03-03 2022-09-09 Voyager Therapeutics, Inc. Controlled expression of viral proteins
US20240141377A1 (en) 2021-03-03 2024-05-02 Voyager Therapeutics, Inc. Controlled expression of viral proteins
WO2023198685A1 (en) 2022-04-13 2023-10-19 F. Hoffmann-La Roche Ag Method for determining aav genomes
GB202205514D0 (en) * 2022-04-13 2022-05-25 Freeline Therapeutics Ltd Mechanical lysis
JP2025517987A (en) 2022-05-23 2025-06-12 エフ. ホフマン-ラ ロシュ アーゲー Raman-Based Methods for Identification of AAV Particle Serotypes and AAV Particle Loading Status
CA3258165A1 (en) 2022-06-03 2023-12-07 F. Hoffmann-La Roche Ag Method for producing recombinant aav particles
AR129895A1 (en) 2022-07-14 2024-10-09 Hoffmann La Roche METHOD FOR PRODUCING RECOMBINANT AAV PARTICLES
WO2024054983A1 (en) 2022-09-08 2024-03-14 Voyager Therapeutics, Inc. Controlled expression of viral proteins
IL318766A (en) 2022-09-12 2025-04-01 Hoffmann La Roche Method for separating full and empty aav particles
IL322001A (en) 2023-02-07 2025-09-01 Hoffmann La Roche Method for the detection of anti-aav particle antibodies
EP4683676A1 (en) 2023-03-21 2026-01-28 F. Hoffmann-La Roche AG Method for the production of recombinant aav particle preparations
WO2024206396A1 (en) * 2023-03-28 2024-10-03 Biogen Ma Inc. Methods of recombinant adeno-associated virus (raav) production
CN120981561A (en) 2023-04-03 2025-11-18 建新公司 Cell lysis device and its usage
WO2024233791A1 (en) 2023-05-11 2024-11-14 Seelos Therapeutics, Inc. Methods of treating neurodegenerative disorders
WO2025168663A1 (en) 2024-02-09 2025-08-14 F. Hoffmann-La Roche Ag Method for producing recombinant adeno-associated viral particles
WO2025252480A1 (en) 2024-06-07 2025-12-11 F. Hoffmann-La Roche Ag Method for purifying plasmid dna
CN119639868B (en) * 2025-02-20 2025-09-23 南京鸿明生物科技有限公司 Gene extraction method for AAV third-generation sequencing

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5837520A (en) 1995-03-07 1998-11-17 Canji, Inc. Method of purification of viral vectors
US6040183A (en) * 1995-06-07 2000-03-21 University Of North Carloina At Chapel Hill Helper virus-free AAV production
JPH11511326A (en) * 1995-08-30 1999-10-05 ジエンザイム コーポレイション Purification of adenovirus and AAV
US6566118B1 (en) * 1997-09-05 2003-05-20 Targeted Genetics Corporation Methods for generating high titer helper-free preparations of released recombinant AAV vectors
US6995006B2 (en) * 1997-09-05 2006-02-07 Targeted Genetics Corporation Methods for generating high titer helper-free preparations of released recombinant AAV vectors
EP1009808B1 (en) * 1997-09-05 2012-12-05 Genzyme Corporation Methods for generating high titer helper-free preparations of recombinant aav vectors
US6146874A (en) 1998-05-27 2000-11-14 University Of Florida Method of preparing recombinant adeno-associated virus compositions
US6416992B1 (en) 1998-10-13 2002-07-09 Avigen, Inc. Compositions and methods for producing recombinant adeno-associated virus
US6221349B1 (en) * 1998-10-20 2001-04-24 Avigen, Inc. Adeno-associated vectors for expression of factor VIII by target cells

Also Published As

Publication number Publication date
US6593123B1 (en) 2003-07-15
WO2002012455A1 (en) 2002-02-14
US20030207439A1 (en) 2003-11-06

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