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AU2001261499A1 - Methods of detecting interactions between proteins, peptides or libraries thereof using fusion proteins - Google Patents

Methods of detecting interactions between proteins, peptides or libraries thereof using fusion proteins

Info

Publication number
AU2001261499A1
AU2001261499A1 AU2001261499A AU6149901A AU2001261499A1 AU 2001261499 A1 AU2001261499 A1 AU 2001261499A1 AU 2001261499 A AU2001261499 A AU 2001261499A AU 6149901 A AU6149901 A AU 6149901A AU 2001261499 A1 AU2001261499 A1 AU 2001261499A1
Authority
AU
Australia
Prior art keywords
proteins
libraries
peptides
methods
detecting interactions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001261499A
Inventor
Indraneel Ghosh
Andrew D. Hamilton
Lynne Regan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yale University
Original Assignee
Yale University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yale University filed Critical Yale University
Publication of AU2001261499A1 publication Critical patent/AU2001261499A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AU2001261499A 2000-05-12 2001-05-14 Methods of detecting interactions between proteins, peptides or libraries thereof using fusion proteins Abandoned AU2001261499A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US20371200P 2000-05-12 2000-05-12
US60203712 2000-05-12
PCT/US2001/015367 WO2001087919A2 (en) 2000-05-12 2001-05-14 Methods of detecting interactions between proteins, peptides or libraries thereof using fusion proteins

Publications (1)

Publication Number Publication Date
AU2001261499A1 true AU2001261499A1 (en) 2001-11-26

Family

ID=22755006

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001261499A Abandoned AU2001261499A1 (en) 2000-05-12 2001-05-14 Methods of detecting interactions between proteins, peptides or libraries thereof using fusion proteins

Country Status (5)

Country Link
US (2) US6780599B2 (en)
EP (1) EP1283846A4 (en)
AU (1) AU2001261499A1 (en)
CA (1) CA2384561A1 (en)
WO (1) WO2001087919A2 (en)

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US7855167B2 (en) * 1998-02-02 2010-12-21 Odyssey Thera, Inc. In vivo screening of protein-protein interactions with protein-fragment complementation assays
US7166424B2 (en) * 1998-02-02 2007-01-23 Odyssey Thera Inc. Fragments of fluorescent proteins for protein fragment complementation assays
AU2001261499A1 (en) 2000-05-12 2001-11-26 Yale University Methods of detecting interactions between proteins, peptides or libraries thereof using fusion proteins
EP1330551A2 (en) * 2000-10-30 2003-07-30 Kalobios, Inc. Affinity maturation by competitive selection
DE10211653A1 (en) * 2002-03-15 2003-10-02 Klaus Pfizenmaier Filament recruitment of fluorescent proteins for analysis and identification of protein-protein interactions: FIT (Filament-based Interaction Trap) analysis
WO2003089464A1 (en) * 2002-04-19 2003-10-30 Bioimage A/S Two green fluorescent protein fragments and their use in a method for detecting protein - protein interactions
AU2003226955A1 (en) * 2002-04-19 2003-11-03 Bioimage A/S Translocation dependent complementation for drug screening
US6878523B2 (en) 2002-05-08 2005-04-12 Gentel Bio Surfaces, Inc. Molecular interaction assays on a solid surface
JP4287633B2 (en) * 2002-09-18 2009-07-01 独立行政法人科学技術振興機構 Analyzing method and materials for organelle localization protein
AU2003301960A1 (en) * 2002-11-05 2004-06-03 Regeneron Pharmaceuticals, Inc. Methods of isolation of active compounds and activated targets
US20040241636A1 (en) * 2003-05-30 2004-12-02 Michnick Stephen William Watson Monitoring gene silencing and annotating gene function in living cells
DE10343375A1 (en) * 2003-09-17 2005-04-28 Axaron Bioscience Ag Method for the detection and analysis of protein-protein interactions
US7488583B2 (en) * 2003-09-25 2009-02-10 Odyssey Thera, Inc. Fragment complementation assays for G-protein-coupled receptors and their signaling pathways
EP1692156B1 (en) 2003-10-24 2012-12-26 Los Alamos National Security, LLC Self-assembling split-fluorescent protein systems
GB0327143D0 (en) * 2003-11-21 2003-12-24 Univ Belfast Assay
WO2005118790A2 (en) * 2004-06-03 2005-12-15 The Trustees Of Columbia University In The City Of New York Combinatorial marking of cells and cell structures with reconstituted fluorescent proteins
US20060094059A1 (en) * 2004-09-22 2006-05-04 Odyssey Thera, Inc. Methods for identifying new drug leads and new therapeutic uses for known drugs
US20070212677A1 (en) * 2004-11-22 2007-09-13 Odyssey Thera, Inc. Identifying off-target effects and hidden phenotypes of drugs in human cells
AU2005314231A1 (en) * 2004-12-04 2006-06-15 The Regents Of The University Of California Protein subcellular localization assays using split fluorescent proteins
JP2008521447A (en) * 2004-12-04 2008-06-26 ザ リージェンツ オブ ザ ユニバーシティー オブ カリフォルニア Protein-protein interaction detection system using fluorescent protein microdomains
US20090149338A1 (en) * 2005-09-30 2009-06-11 Hughes Thomas E System for detecting protein-protein interactions
JP2009526519A (en) * 2006-01-17 2009-07-23 セルーメン、インコーポレイテッド Methods for predicting biological system response
CA2652562C (en) 2006-05-17 2015-05-12 Cellumen, Inc. Method for automated tissue analysis
JPWO2007142208A1 (en) * 2006-06-05 2009-10-22 国立大学法人京都大学 Temperature-sensitive protein, polynucleotide, temperature-sensitive protein expression plasmid and expression cell, and method of using temperature-sensitive protein
US20080299599A1 (en) * 2006-09-07 2008-12-04 Robert Dirksen Fluorescent proteins for monitoring intracellular superoxide production
EP2095119A2 (en) * 2006-11-10 2009-09-02 Cellumen, Inc. Protein-protein interaction biosensors and methods of use thereof
EP2210104A4 (en) * 2007-11-01 2011-02-23 Univ Arizona State ACELLULAR METHODS FOR DETECTING PROTEIN-LIGAND INTERACTIONS
US20100137563A1 (en) * 2008-12-03 2010-06-03 Northwestern University Cysteine Protease Autoprocessing of Fusion Proteins
US8524457B2 (en) 2009-09-22 2013-09-03 William Patterson Method for the selection of specific affinity binders by homogeneous noncompetitive assay
EP2488546B1 (en) * 2009-10-15 2014-12-31 Kemijski Institut Self-assembled structures composed of single polypeptide comprising at least three coiled-coil forming elements
WO2011136602A2 (en) * 2010-04-29 2011-11-03 한국생명공학연구원 Method for selecting antibody-producing cell line, and kit thereof
KR101272817B1 (en) * 2010-04-29 2013-06-10 한국생명공학연구원 Method for Selecting Antibody-producing Cells and Kit for Selecting the Same
ES2739623T3 (en) 2011-03-17 2020-02-03 Cernostics Inc Systems and compositions for diagnosing Barrett's esophagus and methods for using them
DK2790018T3 (en) * 2011-12-05 2017-07-24 Medical & Biol Lab Co Ltd PROCEDURE FOR DETECTING PROTEIN-PROTEIN INTERACTION
WO2018089602A1 (en) * 2016-11-09 2018-05-17 Baylor College Of Medicine Therapeutic for the prevention and/or treatment of weight gain and/or diabetes
CN108732359B (en) * 2017-04-20 2020-09-15 厦门大学 a detection system
KR102541652B1 (en) * 2020-03-31 2023-06-13 바이오디자인랩 주식회사 Safe retroviral vectors developed by insertion of histone or leucine zipper into the viral integrase
CN116075725A (en) 2020-04-17 2023-05-05 瑞泽恩制药公司 Assay for coronavirus neutralizing antibodies
WO2023173028A2 (en) * 2022-03-09 2023-09-14 The Regents Of The University Of Colorado A Body Corporate Tissue and cell-type specific delivery of therapeutic molecules incorporating viral and human fusiogenic proteins

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2196496A1 (en) 1997-01-31 1998-07-31 Stephen William Watson Michnick Protein fragment complementation assay for the detection of protein-protein interactions
US6200762B1 (en) 1997-08-01 2001-03-13 Aurora Biosciences Corporation Photon reducing agents and compositions for fluorescence assays
US6180343B1 (en) 1998-10-08 2001-01-30 Rigel Pharmaceuticals, Inc. Green fluorescent protein fusions with random peptides
DE60043937D1 (en) 1999-06-26 2010-04-15 Odyssey Thera Inc AN IN VIVO "LIBRARY-AGAINST LIBRARY SELECTION" OF OPTIMIZED PROTEIN-PROTEIN INTERACTIONS
AU6058500A (en) * 1999-06-30 2001-01-31 Center For Blood Research, The Fusion protein and uses thereof
AU2001261499A1 (en) 2000-05-12 2001-11-26 Yale University Methods of detecting interactions between proteins, peptides or libraries thereof using fusion proteins

Also Published As

Publication number Publication date
EP1283846A2 (en) 2003-02-19
EP1283846A4 (en) 2005-06-01
CA2384561A1 (en) 2001-11-22
US6780599B2 (en) 2004-08-24
US20020146701A1 (en) 2002-10-10
US7176287B2 (en) 2007-02-13
WO2001087919A3 (en) 2002-05-30
WO2001087919A2 (en) 2001-11-22
US20040235064A1 (en) 2004-11-25

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