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AU2001258400A1 - Method for introducing a nucleic acid into a cell (transfection) by means of calcium phosphate, and transfected cell - Google Patents

Method for introducing a nucleic acid into a cell (transfection) by means of calcium phosphate, and transfected cell

Info

Publication number
AU2001258400A1
AU2001258400A1 AU2001258400A AU5840001A AU2001258400A1 AU 2001258400 A1 AU2001258400 A1 AU 2001258400A1 AU 2001258400 A AU2001258400 A AU 2001258400A AU 5840001 A AU5840001 A AU 5840001A AU 2001258400 A1 AU2001258400 A1 AU 2001258400A1
Authority
AU
Australia
Prior art keywords
cell
introducing
nucleic acid
calcium phosphate
transfection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001258400A
Inventor
Markus Horer
Ulrich Moebius
Bernd Rehberger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Medigene AG
Original Assignee
Medigene AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medigene AG filed Critical Medigene AG
Publication of AU2001258400A1 publication Critical patent/AU2001258400A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention relates to a method for introducing a nucleic acid into at least one cell by means of calcium phosphate (CaPi), to a method for producing a transfected cell and to the transfected cell as such.
AU2001258400A 2000-05-17 2001-05-15 Method for introducing a nucleic acid into a cell (transfection) by means of calcium phosphate, and transfected cell Abandoned AU2001258400A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10024334A DE10024334B4 (en) 2000-05-17 2000-05-17 Method for introducing nucleic acid into a cell (transfection) by means of calcium phosphate
DE10024334 2000-05-17
PCT/EP2001/005531 WO2001088171A1 (en) 2000-05-17 2001-05-15 Method for introducing a nucleic acid into a cell (transfection) by means of calcium phosphate, and transfected cell

Publications (1)

Publication Number Publication Date
AU2001258400A1 true AU2001258400A1 (en) 2001-11-26

Family

ID=7642499

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001258400A Abandoned AU2001258400A1 (en) 2000-05-17 2001-05-15 Method for introducing a nucleic acid into a cell (transfection) by means of calcium phosphate, and transfected cell

Country Status (8)

Country Link
US (1) US20040053410A1 (en)
EP (1) EP1294919B1 (en)
AT (1) ATE333508T1 (en)
AU (1) AU2001258400A1 (en)
CA (1) CA2409701A1 (en)
DE (2) DE10024334B4 (en)
ES (1) ES2266197T3 (en)
WO (1) WO2001088171A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100492292B1 (en) * 2002-11-11 2005-05-27 오덕재 A method for producing calcium-phosphate/nucleic acid complex for transfection
EP2012122A1 (en) 2007-07-06 2009-01-07 Medigene AG Mutated parvovirus structural proteins as vaccines
ES2602610T3 (en) 2007-05-31 2017-02-21 Medigene Ag Mutated structural protein of a parvovirus
ES2742180T3 (en) 2009-03-04 2020-02-13 Deutsches Krebsforsch Assembly activating protein (AAP) and its use for the manufacture of parvovirus particles consisting essentially of VP3
AR082925A1 (en) 2010-09-08 2013-01-16 Medigene Ag STRUCTURAL PROTEINS MUTED BY PARVOVIRUS WITH CROSS-PROTECTED B cell protection epitope, product and related methods
US12487152B2 (en) 2017-05-09 2025-12-02 Ultragenyx Pharmaceutical Inc. Scalable method for producing transfection reagents

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993004169A1 (en) * 1991-08-20 1993-03-04 Genpharm International, Inc. Gene targeting in animal cells using isogenic dna constructs
CN1129955A (en) * 1993-06-29 1996-08-28 奇龙公司 Truncated more bioactive keratinocyte growth factor
AU694800B2 (en) * 1994-09-08 1998-07-30 Genentech Inc. Methods for calcium phosphate transfection
US5976807A (en) * 1998-03-18 1999-11-02 Pharmacopeia, Inc. Eukaryotic cells stably expressing genes from multiple transfected episomes
US20020006664A1 (en) * 1999-09-17 2002-01-17 Sabatini David M. Arrayed transfection method and uses related thereto
US7156579B2 (en) * 2004-09-02 2007-01-02 Clemson University Manufactured caverns in carbonate rock

Also Published As

Publication number Publication date
EP1294919A1 (en) 2003-03-26
CA2409701A1 (en) 2001-11-22
DE10024334A1 (en) 2001-12-06
DE10024334B4 (en) 2006-06-01
WO2001088171A1 (en) 2001-11-22
ATE333508T1 (en) 2006-08-15
EP1294919B1 (en) 2006-07-19
US20040053410A1 (en) 2004-03-18
DE50110507D1 (en) 2006-08-31
ES2266197T3 (en) 2007-03-01

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