[go: up one dir, main page]

ATE80166T1 - Verfahren zur isolierung rekombinanter polypeptide in biologisch aktiver form. - Google Patents

Verfahren zur isolierung rekombinanter polypeptide in biologisch aktiver form.

Info

Publication number
ATE80166T1
ATE80166T1 AT86906674T AT86906674T ATE80166T1 AT E80166 T1 ATE80166 T1 AT E80166T1 AT 86906674 T AT86906674 T AT 86906674T AT 86906674 T AT86906674 T AT 86906674T AT E80166 T1 ATE80166 T1 AT E80166T1
Authority
AT
Austria
Prior art keywords
polypeptide
disulfide bonds
reducing
disulfide
isolation
Prior art date
Application number
AT86906674T
Other languages
English (en)
Inventor
Pradip Ghosh-Dastidar
Original Assignee
Int Genetic Eng
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Int Genetic Eng filed Critical Int Genetic Eng
Application granted granted Critical
Publication of ATE80166T1 publication Critical patent/ATE80166T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/43—Sweetening agents, e.g. thaumatin, monellin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT86906674T 1985-11-13 1986-10-28 Verfahren zur isolierung rekombinanter polypeptide in biologisch aktiver form. ATE80166T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US06/797,473 US4766205A (en) 1985-11-13 1985-11-13 Method for isolation of recombinant polypeptides in biologically active forms
EP86906674A EP0246279B1 (de) 1985-11-13 1986-10-28 Verfahren zur isolierung rekombinanter polypeptide in biologisch aktiver form
PCT/US1986/002264 WO1987002985A1 (en) 1985-11-13 1986-10-28 Method for isolation of recombinant polypeptides in biologically active forms

Publications (1)

Publication Number Publication Date
ATE80166T1 true ATE80166T1 (de) 1992-09-15

Family

ID=25170929

Family Applications (1)

Application Number Title Priority Date Filing Date
AT86906674T ATE80166T1 (de) 1985-11-13 1986-10-28 Verfahren zur isolierung rekombinanter polypeptide in biologisch aktiver form.

Country Status (8)

Country Link
US (1) US4766205A (de)
EP (1) EP0246279B1 (de)
JP (1) JPS63501291A (de)
AT (1) ATE80166T1 (de)
AU (1) AU582927B2 (de)
CA (1) CA1265461A (de)
DE (1) DE3686654T2 (de)
WO (1) WO1987002985A1 (de)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8508340D0 (en) * 1985-03-29 1985-05-09 Creighton T E Production of protein
DE3537708A1 (de) * 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten
CA1310923C (en) * 1985-11-13 1992-12-01 Joachim Ludwig Weickmann Dna encoding [asp --] and [lys -, asp --] thaumatin i
DE3618817A1 (de) * 1986-06-04 1987-12-10 Behringwerke Ag Verfahren zur gewinnung aktiver proteine aus einer biologisch inaktiven form
US4929700A (en) * 1987-04-16 1990-05-29 Cetus Corporation Production of purified, biologically active, bacterially produced recombinant human CSF-1
CA1339757C (en) 1987-04-16 1998-03-17 Robert F. Halenbeck Production of purified biologically active, bacterially produced recombinant human csf-1
US5162507A (en) * 1987-05-11 1992-11-10 Cetus Corporation Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
US4931543A (en) * 1987-05-11 1990-06-05 Cetus Corporation Process for recovering microbially produced interleukin-2
US4985544A (en) * 1987-08-04 1991-01-15 Kyowa Hakko Kogyo Co., Ltd. Process for renaturing fish growth hormone
JPH01132598A (ja) * 1987-10-14 1989-05-25 Pitman Moore Inc 変性剤溶液中に含まれる組換え蛋白質における分子内ジスルフィド結合の生成を促進する方法
US5079230A (en) * 1988-09-12 1992-01-07 Pitman-Moore, Inc. Stable bioactive somatotropins
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
US5221624A (en) * 1988-11-08 1993-06-22 International Genetic Engineering, Inc. DNA encoding (Lys46, Asp97, Asp113) and (Lys46, Asp.sup.137) thaumatin I polypeptides
US5191063A (en) * 1989-05-02 1993-03-02 University Of Medicine And Dentistry Of New Jersey Production of biologically active polypeptides by treatment with an exogenous peptide sequence
IL96682A0 (en) * 1989-12-15 1991-09-16 Amgen Inc Production of biologically active platelet-derived growth factor from high expression host cell systems
US5235043A (en) * 1990-04-06 1993-08-10 Synergen, Inc. Production of biologically active, recombinant members of the ngf/bdnf family of neurotrophic proteins
US5986070A (en) * 1990-04-06 1999-11-16 Amgen Inc. Production of biologically active NGF proteins
EP0545999B1 (de) * 1990-08-20 1997-06-04 Novo Nordisk A/S Prozess für die Herstellung von biologisch aktivem IGF-1 durch Verwendung von amino-terminal verlängertem IGF-1
DE69129747T2 (de) * 1990-09-05 1998-11-12 Southern Cross Biotech Pty. Ltd., Toorak, Victoria In lösung bringen von proteinen in aktiver form
US5235041A (en) * 1990-12-28 1993-08-10 Protein Polymer Technologies, Inc. Purification of structurally ordered recombinant protein polymers
US5326580A (en) * 1993-02-16 1994-07-05 Wisconsin Alumni Research Foundation Brazzein sweetener
EP0628573A1 (de) * 1993-05-24 1994-12-14 Kao Corporation Verfahren zur Herstellung solubilisierten Proteins
WO1995032216A1 (en) * 1994-05-25 1995-11-30 University Of Nebraska Board Of Regents Biologically active glycoprotein hormones produced in procaryotic cells
US5563057A (en) * 1994-10-31 1996-10-08 Wisconsin Alumni Research Foundation Method for refolding misfolded enzymes with detergent and cyclodextrin
US5789547A (en) * 1995-06-07 1998-08-04 Celtrix Pharmaceuticals, Inc. Method of producing insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-3 (IGFBP-3) with correct folding and disulfide bonding
US6342585B1 (en) * 1996-06-11 2002-01-29 Roche Diagnostics Gmbh Method of activating denatured protein
ZA9711733B (en) 1996-12-31 1998-07-01 Monsanto Co Method for solubilization and naturation of somatotropins
US5993627A (en) * 1997-06-24 1999-11-30 Large Scale Biology Corporation Automated system for two-dimensional electrophoresis
US6068993A (en) * 1997-07-02 2000-05-30 Biobras Sa Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin
CZ20032208A3 (cs) * 2001-02-23 2004-01-14 Immunex Corporation Zvýšený výtěžek aktivních proteinů
AU2002349296A1 (en) * 2001-11-30 2003-06-10 Novozymes A/S Method for generating a site-specific library of variants
US20060172376A1 (en) * 2003-02-11 2006-08-03 Hassan Chadjaa Method and device for the continuous production of biomolecules
WO2006047340A2 (en) * 2004-10-22 2006-05-04 Amgen Inc. Methods for refolding of recombinant antibodies
US20100226925A1 (en) * 2007-09-14 2010-09-09 Amgen Inc. Homogeneous Antibody Populations
CN107074968B (zh) 2014-09-01 2021-03-12 国际遗传工程和生物技术中心 疫苗
AR103172A1 (es) 2014-12-22 2017-04-19 Novartis Ag Reducción selectiva de residuos de cisteina en anticuerpos il-17
CN119775339B (zh) * 2025-03-13 2025-09-12 吉尔生化（上海）有限公司 一种提高固相合成的含多个半胱氨酸多肽稳定性的方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3373676D1 (en) * 1982-06-07 1987-10-22 Celltech Ltd A process for the preparation of chymosin
AU538170B2 (en) * 1982-09-15 1984-08-02 Owens-Illinois Glass Container Inc. Modified protein to mould an enzyme type molecule
US4512922A (en) * 1982-12-22 1985-04-23 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
GR79124B (de) * 1982-12-22 1984-10-02 Genentech Inc
US4518526A (en) * 1982-12-22 1985-05-21 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4511503A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4599197A (en) * 1982-12-22 1986-07-08 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4511502A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
JPH06102034B2 (ja) * 1983-03-25 1994-12-14 セルテク リミテツド タンパク質の生産方法
JPS6079000A (ja) * 1983-10-04 1985-05-04 Takeda Chem Ind Ltd ヒトγ型インタ−フエロン単量体の製造法
EP0155832A3 (de) * 1984-03-16 1987-08-19 Genentech, Inc. Stabile Proteine, Verfahren zu deren Herstellung, dieselben kodierende Plasmide und andere DNS, diese Plasmide enthaltende Zellkulturen
US4569790A (en) * 1984-03-28 1986-02-11 Cetus Corporation Process for recovering microbially produced interleukin-2 and purified recombinant interleukin-2 compositions
US4530787A (en) * 1984-03-28 1985-07-23 Cetus Corporation Controlled oxidation of microbially produced cysteine-containing proteins
DE3537708A1 (de) * 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten

Also Published As

Publication number Publication date
AU6591686A (en) 1987-06-02
EP0246279B1 (de) 1992-09-02
US4766205A (en) 1988-08-23
AU582927B2 (en) 1989-04-13
DE3686654D1 (de) 1992-10-08
EP0246279A4 (de) 1989-05-11
CA1265461A (en) 1990-02-06
WO1987002985A1 (en) 1987-05-21
DE3686654T2 (de) 1993-02-18
JPS63501291A (ja) 1988-05-19
EP0246279A1 (de) 1987-11-25

Similar Documents

Publication Publication Date Title
ATE80166T1 (de) Verfahren zur isolierung rekombinanter polypeptide in biologisch aktiver form.
Marder et al. High Molecular Weight Derivatives of Human Fibrinogen Produced by Plasmin: III. THEIR NH2-TERMINAL AMINO ACIDS AND COMPARISON WITH THE “NH2-TERMINAL DISULFIDE KNOT
DE3650751D1 (de) Antikörper gegen Saugetier-Interleukin-4 und Peptide verwendbar als Antigenen für deren Herstellung
ES2020498T3 (es) Procedimiento para la activacion de proteinas eucarioticas heterologas, que tienen puentes de disulfuro, preparadas por tecnologia genetica, despues de su expresion en el seno de procariotas.
DE69112207D1 (en) "walk-through"-mutagenese.
ATE123949T1 (de) B-ketten-analoge des von blutplättchen abstammenden wachstumsfaktors und verfahren zu deren homogener herstellung.
ES8802235A1 (es) Procedimiento para preparar un polipeptido
AU5385486A (en) Biologically active protein compositions and a method of use
DE3689386D1 (de) Proteaseresistente Urokinase-Zusammensetzung, deren Herstellung und Verwendung.
ES2065907T3 (es) Procedimiento para la produccion de un peptido fisiologicamente activo que contiene un resto cisteina.
ES548617A0 (es) Procedimiento para disociar peptidos y proteinas en el enla-ce metionilo
DK390689A (da) Fremgangsmaade til fremstilling af des-b30-insuliner og des-b30-insulinderivater
DE3777845D1 (de) Pharmazeutischer stoff fuer die behandlung von myelogener leukaemie.
PT93178A (pt) Agente proteico anticanceroso
ES2124308T3 (es) Composicion estable de polipeptidos.
DE69121489D1 (de) In vitro-Behandlung von Fusionsproteinen
DE3852588D1 (de) Biologisch aktive Moleküle.
SE9101586D0 (sv) Modified thioredoxin and its use

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee