[go: up one dir, main page]

ATE168194T1 - Molekulargewichtstrennung von biomolekülen durch kapillare elektrophorese unter verwendung einer polymerhaltigen lösung - Google Patents

Molekulargewichtstrennung von biomolekülen durch kapillare elektrophorese unter verwendung einer polymerhaltigen lösung

Info

Publication number
ATE168194T1
ATE168194T1 AT93910939T AT93910939T ATE168194T1 AT E168194 T1 ATE168194 T1 AT E168194T1 AT 93910939 T AT93910939 T AT 93910939T AT 93910939 T AT93910939 T AT 93910939T AT E168194 T1 ATE168194 T1 AT E168194T1
Authority
AT
Austria
Prior art keywords
solution containing
molecular weight
biomolecules
capillary electrophoresis
containing polymers
Prior art date
Application number
AT93910939T
Other languages
English (en)
Inventor
David M Demorest
William E Werner
John E Wiktorowicz
H-Michael Wenz
Frank L Oaks
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Application granted granted Critical
Publication of ATE168194T1 publication Critical patent/ATE168194T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44752—Controlling the zeta potential, e.g. by wall coatings
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Macromolecular Compounds Obtained By Forming Nitrogen-Containing Linkages In General (AREA)
AT93910939T 1992-05-01 1993-04-30 Molekulargewichtstrennung von biomolekülen durch kapillare elektrophorese unter verwendung einer polymerhaltigen lösung ATE168194T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/877,956 US5264101A (en) 1989-11-06 1992-05-01 Capillary electrophoresis molecular weight separation of biomolecules using a polymer-containing solution

Publications (1)

Publication Number Publication Date
ATE168194T1 true ATE168194T1 (de) 1998-07-15

Family

ID=25371084

Family Applications (1)

Application Number Title Priority Date Filing Date
AT93910939T ATE168194T1 (de) 1992-05-01 1993-04-30 Molekulargewichtstrennung von biomolekülen durch kapillare elektrophorese unter verwendung einer polymerhaltigen lösung

Country Status (6)

Country Link
US (1) US5264101A (de)
EP (1) EP0638168B1 (de)
JP (1) JP3176373B2 (de)
AT (1) ATE168194T1 (de)
DE (1) DE69319596T2 (de)
WO (1) WO1993022665A1 (de)

Families Citing this family (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5332481A (en) 1991-01-29 1994-07-26 Beckman Instruments, Inc. Capillary electrophoresis using replaceable gels
US5384024A (en) * 1992-03-13 1995-01-24 Applied Biosystems, Inc. Capillary electrophoresis
JP3012003B2 (ja) * 1993-10-14 2000-02-21 バイオ−ラッド ラボラトリーズ，インコーポレイティド 荷電したポリマーを用いることによる、ゲルのないポリマー媒体における電気泳動の間の電気浸透の抑制
US5891313A (en) * 1993-11-23 1999-04-06 The Perkin-Elmer Corp. Entrapment of nucleic acid sequencing template in sample mixtures by entangled polymer networks
WO1995016910A1 (en) * 1993-12-17 1995-06-22 Perkin-Elmer Corporation Uncharged polymers for separation of biomolecules by capillary electrophoresis
US6358385B1 (en) * 1993-12-17 2002-03-19 The Perkin-Elmer Corporation Polymers for separation of biomolecules by capillary electrophoresis
US5423966A (en) * 1994-01-25 1995-06-13 Perkin-Elmer Corporation On line ion contaminant removal apparatus and method for capillary electrophoresis
US5503722A (en) * 1994-02-28 1996-04-02 Beckman Instruments, Inc. Rehydratable gels for capillary electrophoresis
DE69633962T2 (de) 1995-01-27 2005-12-01 Northeastern University, Boston Verfahren zum Bilden einer kovalent gebundenen hydrophilen Schicht auf Basis von Polyvinyl-Alkohol für die Kapillarelektrophorese
US5582705A (en) * 1995-05-19 1996-12-10 Iowa State University Research Foundation, Inc. Multiplexed capillary electrophoresis system
EP0870194A1 (de) * 1995-12-28 1998-10-14 Novartis AG Elektroforetisches kapillartrennverfahren unter verwendung einer füllbaren polymerlösung als trennmittel
US5942443A (en) 1996-06-28 1999-08-24 Caliper Technologies Corporation High throughput screening assay systems in microscale fluidic devices
US6001232A (en) * 1996-09-04 1999-12-14 The Research Foundation Of State University Of New York Separation medium for capillary electrophoresis
US5989399A (en) * 1996-09-04 1999-11-23 The Research Foundation Of State University Of New York Effective surface treatment for a new separation medium in electrophoresis
US5964995A (en) 1997-04-04 1999-10-12 Caliper Technologies Corp. Methods and systems for enhanced fluid transport
US5976336A (en) * 1997-04-25 1999-11-02 Caliper Technologies Corp. Microfluidic devices incorporating improved channel geometries
US7033474B1 (en) 1997-04-25 2006-04-25 Caliper Life Sciences, Inc. Microfluidic devices incorporating improved channel geometries
AU727083B2 (en) 1997-04-25 2000-11-30 Caliper Life Sciences, Inc. Microfluidic devices incorporating improved channel geometries
US5958694A (en) * 1997-10-16 1999-09-28 Caliper Technologies Corp. Apparatus and methods for sequencing nucleic acids in microfluidic systems
US5948227A (en) 1997-12-17 1999-09-07 Caliper Technologies Corp. Methods and systems for performing electrophoretic molecular separations
US6013165A (en) * 1998-05-22 2000-01-11 Lynx Therapeutics, Inc. Electrophoresis apparatus and method
AU747464B2 (en) 1998-06-08 2002-05-16 Caliper Technologies Corporation Microfluidic devices, systems and methods for performing integrated reactions and separations
US6706161B1 (en) * 1998-06-10 2004-03-16 Agilent Technologies, Inc. Methods and compositions for modifying the surface of a polymeric substrate
US6800287B2 (en) * 1998-09-25 2004-10-05 Yeda Research And Development Co., Ltd. Copolymer 1 related polypeptides for use as molecular weight markers and for therapeutic use
AU758309B2 (en) 1999-02-02 2003-03-20 Caliper Life Sciences, Inc. Methods, devices and systems for characterizing proteins
US6599836B1 (en) * 1999-04-09 2003-07-29 Micron Technology, Inc. Planarizing solutions, planarizing machines and methods for mechanical or chemical-mechanical planarization of microelectronic-device substrate assemblies
US6407000B1 (en) * 1999-04-09 2002-06-18 Micron Technology, Inc. Method and apparatuses for making and using bi-modal abrasive slurries for mechanical and chemical-mechanical planarization of microelectronic-device substrate assemblies
US6306273B1 (en) * 1999-04-13 2001-10-23 Aclara Biosciences, Inc. Methods and compositions for conducting processes in microfluidic devices
US6716948B1 (en) * 1999-07-31 2004-04-06 Symyx Technologies, Inc. Controlled-architecture polymers and use thereof as separation media
US6680208B1 (en) * 1999-11-19 2004-01-20 Becton, Dickinson And Company Rapid protein identification using antibody mixtures
US20020012971A1 (en) * 2000-03-20 2002-01-31 Mehta Tammy Burd PCR compatible nucleic acid sieving medium
JP2003532073A (ja) * 2000-04-25 2003-10-28 スペクトラメディックス コーポレイション 高温条件下での生体分子の変性剤−フリー電気泳動
FR2810905B1 (fr) * 2000-06-30 2003-05-16 Inst Curie Additif pour minimiser les phenomenes d'adsorption et/ou d'electroosmose
US6706162B1 (en) * 2000-09-25 2004-03-16 Applera Corporation High speed, high resolution compositions, methods, and kits for capillary electrophoresis
WO2002101375A2 (en) * 2001-06-13 2002-12-19 Symyx Technologies, Inc. Polymer additive for use in separation media
JP4657524B2 (ja) * 2001-08-29 2011-03-23 嘉信 馬場 マイクロチップ電気泳動用装置及びそれを用いる電気泳動方法
US20050130157A1 (en) * 2002-01-22 2005-06-16 Philip Serwer Electrophoretic ratchets and cyclic electrophoresis
US20040029143A1 (en) * 2002-02-28 2004-02-12 Jeffrey Van Ness Cationic polyelectrolytes in biomolecule purification and analysis
DE10243930A1 (de) * 2002-03-08 2003-09-25 Conducta Endress & Hauser Vorrichtung zur Messung und Regelung des pH-Wertes eines Mediums
US7381317B2 (en) * 2002-08-12 2008-06-03 Beckman Coulter, Inc. Methods and compositions for capillary electrophoresis (CE)
US7083711B2 (en) * 2003-02-21 2006-08-01 Combisep, Inc. Capillary electrophoresis gel especially for separation made for single stranded nucleic acid separations
US20050025741A1 (en) 2003-05-15 2005-02-03 Lau Aldrich N.K. Poly and copoly(N-vinylamide)s and their use in capillary electrophoresis
WO2004106888A2 (en) * 2003-05-22 2004-12-09 Spectrumedix Llc Electrophoresis capillaries having reduced amounts of -oh
JP4450368B2 (ja) 2004-03-04 2010-04-14 独立行政法人産業技術総合研究所 マイクロ流路チップの製造方法、マイクロ流路チップ、そのマイクロ流路チップを用いる生体分子の分離方法、およびそのマイクロ流路チップを有する電気泳動装置
US7935479B2 (en) * 2004-07-19 2011-05-03 Cell Biosciences, Inc. Methods and devices for analyte detection
US7846676B2 (en) 2004-07-19 2010-12-07 Cell Biosciences, Inc. Methods and devices for analyte detection
KR100647306B1 (ko) * 2004-12-23 2006-11-23 삼성전자주식회사 아미노기와 카르복실기를 포함하고 제1 ｐＨ에서 양전하를띠는 물질을 이용하여 핵산을 분리하는 방법
JP4645255B2 (ja) * 2005-03-24 2011-03-09 株式会社島津製作所 分析試料調製用溶媒
US8481125B2 (en) * 2005-05-21 2013-07-09 Advanced Liquid Logic Inc. Mitigation of biomolecular adsorption with hydrophilic polymer additives
US20070014699A1 (en) * 2005-06-23 2007-01-18 Beckman Coulter, Inc, Methods and apparatus for improving the sensitivity of capillary zone electrophoresis
JP4167296B2 (ja) * 2005-08-01 2008-10-15 株式会社ナード研究所 電気泳動用ポリアクリルアミドゲル、それを用いたポリアクリルアミドゲル電気泳動方法、その製造方法、およびアクリルアミド化合物
US8945361B2 (en) 2005-09-20 2015-02-03 ProteinSimple Electrophoresis standards, methods and kits
US10107782B2 (en) 2008-01-25 2018-10-23 ProteinSimple Method to perform limited two dimensional separation of proteins and other biologicals
US20100258439A1 (en) * 2009-04-14 2010-10-14 Huang Lynn Ling-Huei Capillary electrophoresis method for analyzing collagen and an assay kit for the same
US9841401B2 (en) 2009-04-14 2017-12-12 National Cheng Kung University Capillary electrophoresis method for analyzing collagen
US9442091B2 (en) 2011-01-31 2016-09-13 Qiagen Mansfield, Inc. Reduced artifact denaturing capillary electrophoresis of nucleic acids
EP2839276B1 (de) 2012-04-19 2018-06-27 ProteinSimple Isoelektrische fokussierung mit zwei wellenlängen zur bestimmung der wirkstofflast in antikörper-wirkstoff-konjugaten
JP6348258B2 (ja) * 2013-06-20 2018-06-27 大塚電子株式会社 分離分析方法
US9766206B2 (en) 2013-09-27 2017-09-19 ProteinSimple Apparatus, systems, and methods for capillary electrophoresis
WO2018051374A1 (en) * 2016-09-15 2018-03-22 Dianax S.R.L. Microfluidic device, particularly of the lab-on-chip type, for the concentration, purification, and detection of biological and/or medical targets of interest from a biological sample
US11946900B2 (en) * 2018-04-09 2024-04-02 Shimadzu Corporation Electrophoresis flow channel cleaning method and electrophoresis device
US12203130B2 (en) * 2018-12-20 2025-01-21 Krylov Sergey N Binder selection using capillary electrophoresis
EP3757176B1 (de) * 2019-06-27 2022-08-31 Canon Production Printing Holding B.V. Tintenstrahlbeschichtungszusammensetzung
US10985617B1 (en) 2019-12-31 2021-04-20 Energous Corporation System for wirelessly transmitting energy at a near-field distance without using beam-forming control
JP2025508886A (ja) * 2022-02-28 2025-04-10 レヴィティ・ヘルス・サイエンシズ・インコーポレイテッド 二本鎖及び一本鎖リボ核酸（ｒｎａ）を分離並びに検出する方法
WO2024088984A1 (en) * 2022-10-25 2024-05-02 Synthon B.V. Capillary gel electrophoresis separation of diastereomers of phosphorothioated oligonucleotides

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4405798A (en) * 1981-03-30 1983-09-20 Celanese Corporation Acrylate and methacrylate monoesters of pentaerythritol and pentaerythritol orthoesters and polymers and copolymers derived therefrom
JPS6060548A (ja) * 1983-09-14 1985-04-08 Fuji Photo Film Co Ltd 電気泳動用媒体
JPS6060549A (ja) * 1983-09-14 1985-04-08 Fuji Photo Film Co Ltd ゲル電気泳動用媒体
US4690749A (en) * 1985-12-16 1987-09-01 Universities Space Research Association Polymer-coated surfaces to control surface zeta potential
US4997537A (en) * 1986-10-21 1991-03-05 Northeastern University High performance microcapillary gel electrophoresis
US4931328A (en) * 1988-08-19 1990-06-05 Hewlett-Packard Company Capillary tube with reduced protein interactions and controllable electroosmotic flow
EP0417925A3 (en) * 1989-09-12 1991-09-11 Northeastern University High performance microcapillary gel electrophoresis
DE69007773T2 (de) * 1990-02-13 1994-09-22 Bio Rad Laboratories Elektrophoretische Trennung in einem gelfreien Medium mittels gelösten Polymeren.
US5069766A (en) * 1990-12-20 1991-12-03 Bio-Rad Laboratories, Inc. Suppression of electroendosmosis in capillary electrophoresis

Also Published As

Publication number Publication date
WO1993022665A1 (en) 1993-11-11
EP0638168B1 (de) 1998-07-08
JP3176373B2 (ja) 2001-06-18
US5264101A (en) 1993-11-23
JPH07506432A (ja) 1995-07-13
DE69319596T2 (de) 1999-03-04
EP0638168A4 (de) 1995-07-19
DE69319596D1 (de) 1998-08-13
EP0638168A1 (de) 1995-02-15

Similar Documents

Publication Publication Date Title
ATE168194T1 (de) Molekulargewichtstrennung von biomolekülen durch kapillare elektrophorese unter verwendung einer polymerhaltigen lösung
SE9602638D0 (sv) An improved method for the capillary electrophoresis of nucleic acids, proteins and low molecular charged compounds
DK260485A (da) Kationisk polyelektrolyt med hoej molekylvaegt samt fremgangsmaade til fremstilling deraf
DE69033795D1 (de) Oberflächenladungsbeschichtung für kapillare elektrophorese
NO158877C (no) Fremgangsmaate for fremstilling av monomere eller polymereforbindelser med isocyanursyregrupper.
DE3674197D1 (de) Wasserabsorbierende acrylcopolymere.
PT83351A (en) Process for the preparation of a composition based in an aqueo us dispersion of an addition polymer
DE69226167D1 (de) Strukturgen von pneumokokken-protein
KR880013890A (ko) 가교 결합 이미도페닐아민(Imidophenylamines)
ES2053711T3 (es) Electrolito polimerico solido y generadores electroquimicos que lo contengan.
NO179000C (no) Betongtaksten og fremgangsmåte for fremstilling derav
KR890003847A (ko) 중합성 기판 제품
DE3681569D1 (de) Dispersionspolymere, verfahren zu ihrer herstellung und ihre verwendung.
DE3683656D1 (de) Potentialverursachendes element fuer einen immunosensor.
KR870005703A (ko) 전착 도장(Electrodeposition coating) 방법
EP0209830A3 (de) Acrylmodifizierte Polymere
ATE39259T1 (de) Wasserabsorbierende harze, verfahren zu deren herstellung und deren verwendung bei der herstellung von waessrigen fluidaabsorbierenden gegenstaenden.
SE8105599L (sv) Framstellning av hydrofila polymergeler med hog molekylvikt
KR840001446A (ko) 금속제 병의 제조방법
ES2088633T3 (es) Estabilizantes polisiloxanicos que contienen grupos fenol y grupos oxamidicos estericamente impedidos.
ES516049A0 (es) "un metodo de mejorar la resistencia a la arborescencia en agua de un polimero de etileno".
KR870006139A (ko) 도금용 폴리페닐렌 설파이드 수지 조성물
FI871957L (fi) Foerfarande foer framstaellning av polymerer och kopolymerer av amidosulfonsyra innehaollande monomerer och deras salter.
FR2509912B1 (fr) Pile electro-chimique au calcium
DE3650293D1 (de) Vorrichtung zur Bestimmung der Senkungsgeschwindigkeit von Blutzellen.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties