AT508394A1 - DEVICE AND METHOD FOR THE APPLICATION OF RINGSTROMINDUCTION MODULATED BY MAGNETIC FIELD VARIATION IN ELECTRICALLY CONDUCTIVE NANO PARTICLES FOR MECHANICAL IMPACT ON CELLULAR MEMBRANES - Google Patents

Description

• • • • Μ 12776 • · ··· • ♦ · · ♦ · · · ♦. * · ·

1

Apparatus and method for the application of magnetic field variation modulated Ringstrominduktion in electrically conductive nano-particles for mechanical action on cellular membranes

The invention relates to a method for rupturing cell walls of selected cells as well as a device and a docking substance for use in the method.

In recent years, various research projects have investigated potential applications of so-called nanoparticles. Nano particles are small particles whose physical size is in the nanometer range. Due to their small size, nanoparticles can be incorporated into other substances or bodies (carrier material). Depending on the physical properties of the nanoparticles and the interaction of the nanoparticles with the carrier material, effects or changes in the carrier material can be achieved.

In medical research projects in the field of cell research, the interaction of cells or cell types with other cells or cell types has been investigated in recent years. In particular, it was also researched what conditions must be met so that certain cells or cell parts or molecules or other organic elements (hereinafter referred to as docking body) attach themselves to other cells or cell parts or dock. For example, it has been possible to produce antibodies for the healing of certain diseases which, by virtue of their properties, increase or almost exclusively attach to certain cells which are associated with the disease. Thus, the antibodies can target the disease causing cells. However, even in laboratory or laboratory tests, the docking of certain test-dependent docking bodies (e.g., paint indication colorant) to cells is successful.

It is known that magnetic fields exert attraction and / or repulsion forces on bodies with ferromagnetic properties. It is also known that a magnetic field in electrically conductive materials induces an electric current, which in turn generates an electromagnetic field. Since this induced by the induced current electromagnetic field is very weak compared with the actual triggering magnetic field, its effect on the ferromagnetic effect can be neglected in everyday objects.

It is an object of the present invention to profitably apply research results from entirely different fields of research for mankind in medical technology and other fields. It is a further object of the present invention to provide a method and apparatus for facilitating further advances in the field of cell research. In particular, the invention is based on the object of damaging or destroying certain cells in an environment medium having different cells.

In order to achieve the abovementioned object, in a method for rupturing cell walls of selected cells, the following method steps are carried out, wherein in a first method step a docking substance of docking bodies provided with electrically conductive nano-particles, which due to their properties only to certain Dock cells, is introduced into an environment medium having different cells, and wherein, in a second method step, the docking bodies dock onto these specific and thus selected cells and wherein in a third method step an alternating magnetic field is imprinted into the nano-cell. Particles induces a ring current whose ring current magnetic field interacts with the impressed alternating magnetic field and mechanically brings the nano-particles to vibrate until thereby acting on the cell walls of the selected cells mechanical forces tearing the cell walls.

In order to achieve the aforementioned object in a device for rupturing cell walls of selected cells according to the above method, the following structure according to the invention is chosen:

A coil for generating an alternating magnetic field and having a current source for delivering a direct current and with a

Signal modulator for modulating the direct current with a modulation signal output by a frequency generator and outputting a coil driving signal to the coil and with one of the coil connected in parallel with the coil ···· ·· ···· »·· · · · · · · · · · •··············································································································································································································································· stored energy at the falling edges of the modulation signal.

To solve the above-mentioned problem with a docking substance for use in the above method, the following composition is selected. In the docking substance are provided with electrically conductive nano-particles docking body, which dock due to their properties only to certain cells, provided, the docking substance is introduced into a different cells having environmental medium and wherein the docking body are docked in this environment and thereby selected cells contained in the ambient medium and wherein by impressing an alternating magnetic field in the ambient medium, in the nano-particles, a ring current is inducible whose ring current magnetic field interacts with the impressed alternating magnetic field and the nano- Particles in mechanical wings are displaceable until the forces acting on the cell walls of the selected cells mechanical forces are formed to rupture the cell walls.

The invention utilizes the physical phenomenon that an imprinted alternating magnetic field induces ring currents in conductive bodies and that these ring currents themselves produce a magnetic self field or ring current magnetic field that is independent of the ferromagnetic properties of the conductive body. The strength of these ring currents is dependent on the ohmic resistance, which in addition to the conductivity of the material of the body is essentially determined by the distance along which the current must flow. For bodies with a small surface area - which is the case with nanoparticles - this distance is correspondingly short and thus the ohmic resistance correspondingly small. For larger bodies of the ohmic resistance is greater by the larger current up to several orders of magnitude and the strength of the ring currents correspondingly smaller. As a result, the ring current magnetic field in nano-particles is relatively strong for their size. Nevertheless, the ring current magnetic field determining the intensity of the mechanical vibrations is weak compared to the impressed alternating magnetic field. However, it is essential that the flux density acting on the spatial volume of this ring current magnetic field acts on the spatial cross section of the triggering nanoparticle, whereby the mechanical surface tension is concentrated on the nanoparticles. As a result, the strength of the interaction of the ring current magnetic field with the impressed alternating magnetic field in nano-particles exceeds the strength of the interaction of the impressed magnetic

Alternating field with an optionally present ferromagnetic property of the nano-particle.

By suitable for the particular application selection of docking bodies and the doping of the docking body with nano-particles, which are electrically conductive, a substance is created with the tailored for the application, a certain type of cells in the ambient medium by the process of tearing the cell walls can be destroyed. This substance is introduced into the ambient medium and, depending on the nature of the docking body, waited a certain predetermined time until essentially all docking bodies have docked to the specific type of cells. Subsequently, by impressing the magnetic alternating field suitable for the application and utilizing the phenomenon described above for nano-particles, the specific cells selected are destroyed. The mechanical vibrations of the nano-particles are excited by the corresponding shape and frequency of the magnetic field pulses of the impressed alternating magnetic field such that the entire docking body starts to vibrate and the cell wall at the point where the docking body has docked to the cell , Is so mechanically loaded until the cell wall tears or ruptures. A cell with an open cell wall is subsequently no longer viable and dies.

Previously known methods for destroying certain types of cells took advantage of the fact that cells are viable only up to certain temperatures, which is why areas in the surrounding medium in which cells to be destroyed are frequently heated locally. By means of this thermal action, however, a large number of non-selected cells, which are also present in this superheated area in the ambient medium, are also destroyed in the known methods, as a result of which entire areas die off in cell cultures or bodies. One of the particular advantages of the method according to the invention, the device according to the invention and the ambient medium according to the invention for rupturing cell walls of selected cells is that the selected cells are destroyed by mechanical processes acting specifically only on the selected cells. The consequence of this is that adjacent non-selected cells to which selected cells have not docked to which docking bodies are not harmed in any way and therefore only the cells to be destroyed are specifically affected.

Due to the ring current occurring in the nano-particles, the nano-particles are heated, which in turn leads to an increase in the ohmic resistance of the nano-particles and a consequent even greater heating of the nano-particles. In the method and the device according to the invention has proved to be advantageous to impress a magnetic alternating field in the low frequency range. This frequency range is best suited to give the nano-particles enough time to cool down, which will be discussed in more detail below. High-frequency excitations have the disadvantage that only low field strengths are transferable and that, above all, only the fine structure within the selected cells is excited due to the inductive resistance. Although this energy is transported, which manifests itself as heat, but does not induce significant macroscopic kinematics.

It is furthermore advantageous in the method according to the invention and the device according to the invention to provide input means of the device with which the duty cycle - magnetic field present or not present - of the impressed magnetic alternating field to a large number of different influencing factors (eg type of selected cells, type of used Docking body, type and size of nano-particles, ...) of the particular application can be adjusted.

In experiments, it has been found that it is particularly advantageous to impress trapezoidal magnetic field pulses. On steep rising edges, a high ring current is generated in the nano-particle, which, during the subsequent magnetic field plateau of the magenta field pulse, leads to a strong mechanical deflection of the nano-particle. The subsequent descent edge and zero phase of the magnetic field pulse is used to cool the nano-particle. The flux density of the impressed alternating magnetic field during times of the magnetic field plateau of the trapezoidal magnetic field pulse should have at least 30 milli-Tesla [mT], with 50 milli-Tesla being particularly advantageous.

Furthermore, it is particularly advantageous not to impress a homogeneous magnetic field which is as inhomogeneous as possible, since in this way particularly strong ring currents are established in the nano-particles. Such a highly inhomogeneous alternating magnetic field can be advantageously generated by providing two coils in the device according to the invention, when oppositely polarized drive signals are fed into the coils substantially simultaneously.

With knowledge of the invention, the person skilled in the art can imagine a large number of different docking bodies in this context according to the invention, which are selected depending on the particular application. For laboratory tests or others

Use cases are antibodies as docking body advantageously used, since such antibodies have been used for many years.

It can be mentioned that the method according to the invention and the device according to the invention and the ambient medium according to the invention can be used in a large number of medical and non-medical application cases, as will be explained in more detail in the following exemplary embodiments.

FIG. 1 shows a nano-particle on which an impressed magnetic field acts.

FIG. 2 shows the nano-particle according to FIG. 1, wherein it is considered in the direction of the magnetic field lines of the impressed magnetic field.

FIG. 3 shows a schematic representation of a cell together with the docked antibody provided with a nano-particle.

FIG. 4 shows a schematic representation of the cell according to FIG. 3, in which the cell wall was torn open by mechanical oscillations of the nanoparticle.

FIG. 5 shows a block diagram of a device for impressing an alternating magnetic field.

FIG. 6 shows the penetration of the impressed alternating magnetic field into the ambient medium.

FIG. 7 shows three examples of advantageous outer and inner diameters of coils of the device according to FIG. 5.

FIG. 8 schematically shows a sectional view through a coil which can be used in the device according to FIG. 5 and has two spiral windings lying next to one another.

FIG. 9 schematically shows a sectional view through a coil which can be used in the device according to FIG. 5, with a spiral winding of rectangular shaped wrapping material.

FIG. 10 shows a symmetrical trapezoidal profile of the coil drive signal applied to the coil of the device according to FIG. 5 for generating the impressed alternating magnetic field.

FIG. 11 shows an asymmetrical trapezoidal profile of the coil drive signal applied to the coil of the device according to FIG. 5 for generating the impressed alternating magnetic field. ····· ν · »« · ································ ····· ν · · · · · ··· · ··

FIG. 1 shows a nanoparticle 1 whose diameter is two nanometers and which is formed from electrically conductive material. The nano-particle 1 is a commercially available as so-called nano-beed on the market iron chip, the expert nano-particles are known from a variety of electrically conductive materials of various shapes, the extent of which can range from only a few tenths of a nanometer to a few microns ,

An imprinted magnetic field acts on the nano-particle 1, of which a magnetic field line H is shown transversely through the nano-particle 1 in FIG. The magnetic field induces an induction current in the nano-particle 1, which forms as ring current R. The ring current R in turn generates a ring current magnetic field, of which a first magnetic field line RH1 and a second magnetic field line RH2 are shown in FIG. Due to the interaction of the impressed magnetic field with the ring current magnetic field acts according to Lenz's rule, a mechanical force F in the direction of the magnetic field line H of the embossed magnetic field on the nano-particles 1, which would like to move the nano-particle 1 in the direction shown. While the flux density (measured in Tesla) is relevant for the strength of the resulting mechanical force F in the impressed magnetic field, the magnetic field strength (measured in amperemeter) is relevant for the ring current magnetic field. The force F acting on the entire ring current magnetic field of the nano-particle 1 is concentrated on the cross-section of the nano-particle 1.

In FIG. 2, the nanoparticle 1 is shown viewed in the direction of the magnetic field line H of the impressed magnetic field, wherein it has a spherical body having a circular spatial extension transverse to the direction of the magnetic field line H. This defines the area cross section of the nanoparticle 1. The ring current magnetic field generated by the ring current R occupies a spatial volume which is greater than the nano particle 1 itself and thus takes in the same perspective, a larger magnetically effective area cross section.

FIG. 3 is a schematic representation of a cell 2 having a cell wall 3 and a cell nucleus 4 surrounded by genetic material 5. A cell such as cell 2 is viable only so long as the cell wall 3 is undamaged and prevents leakage of the genetic material 5. Cell 2 has a certain structure «! on the surface of ········································································

Cell wall 3, which are characteristic of the type of cell 2. The person skilled in the art is aware of certain docking bodies that only attach or dock at specific structures on the surface of specific cells. Thus, by selecting the appropriate docking bodies, a particular type of cell (e.g., plant cell or animal cell or fungal cell) to be acted upon by the method of the invention may be selected.

FIG. 3 further shows an antibody 6 provided with a nanoparticle 1 which, in the given application, forms a docking body, wherein the antibody 6 according to FIG. 3 has already been docked to the cell wall 3 of the cell 2. By docking the antibody 6 to the cell 2, the cell 2 has been selected for the further process. By providing the antibody 6 with the nano-particle 1, the nano-particle 1 was mechanically inseparably bound to the antibody 6 in a manner not described in detail here. The person skilled in the art is aware of methods for providing or doping docking bodies with nanoparticles.

FIG. 4 now shows the cell 2 in which the cell wall 2 at the point at which the antibody 6 was docked on the cell wall 2 has been torn open by mechanical vibrations of the nanoparticle 1. As explained with reference to FIG. 1, an impressed magnetic field generates a force F in the direction of the magnetic field line H of the impressed magnetic field. According to the invention, an alternating magnetic field is now impressed, which alternately periodically exerts a force F on the nano-particle 1 and on the antibody 6 on the cell wall 3 of the cell 2. The antibody 6 was chosen so that it docks very tightly on the cell wall 3, which is why the cell wall 3 in the region where the antibody 6 docks, the weakest link in the chain and with continuously impressed mechanical vibrations first yields and tears the cell wall 3 ,

It may be mentioned that the directions in which the antibodies 6 docked on the cell wall 3 are arbitrary relative to the impressed alternating magnetic field. This means that a certain part of the antibody 6 penetrates the cell wall 3 more or less vertically, as shown in FIG. 4, while in the larger part of the selected cells 2 the cell wall 3 is torn laterally. Since also in the case of material failure due to compressive and tensile stresses, the shear forces occurring in the inner compressive or tensile deformation are relevant, the force F necessary for rupturing the cell wall 3 is comparable in all cases. • »« • * • • • • • • · · • • 0 • # * * • 0 ·· · • •

FIG. 5 shows a block diagram of a device 7 for tearing cell walls 3 of selected cells 2. The device 7 has a current source 8 for delivering a direct current I to a signal modulator 9, the current source 8 providing sufficient current even at low voltage. The signal modulator 9 is configured to modulate the DC current I with a modulation signal MS output from a frequency generator 10 and to output a coil drive signal SA to a coil S1. The coil S1 is formed by a single spiral winding and is designed to generate an alternating magnetic field MW shown in FIG. 6, which acts on selected cells 2 contained in an ambient medium UM.

The construction and dimensioning of the coil 1 is essential for the operation of the device 7. Depending on the particular application, the magnetic alternating field should rather be impressed locally and deeply or rather over a large area and concentrated at the surface into the ambient medium UW. In order to ensure the desired inhomogeneity of the alternating magnetic field as a function of the radial dimensioning of the coil 1, the thickness of the coil, i. the height of the cylinder can be set according to the radial dimensioning. Outer diameters of coils S usable in the device 7 may vary, for example, in the range between 10 cm and 20 cm, while the inner ring diameter may be between 1 cm and 4 cm. FIG. 7 essentially shows an advantageous radial dimensioning with inner and outer diameters of coils S which can be used in the device 7 for generating the magnetic alternating field MW. At the proposed diameters, the depth of the coil 1 should be between 1 cm and 2 cm. Depending on the application, the dimensions of the coil S may also differ materially from these specifications.

Figure 8 shows a usable in the device 7 according to Figure 5 Spille S2 with two adjacent spiral windings. It is particularly advantageous to feed two substantially simultaneously oppositely poled coil drive signals SA into the two windings of the coil S2, whereby a particularly inhomogeneous alternating magnetic field MW is impressed in the nano-particles 1. As a result, particularly strong ring currents R are induced, which lead to strong mechanical vibrations of the nano-particles 1. Also advantageous for generating the inhomogeneous magnetic alternating field MW is the use of a coil S3 shown in Figure 9 with only one spiral winding of rectangular shaped winding material. Those skilled in the technical field of the coils are more in a device 7 according to the invention for the • · • · · · · · ···

1" • · • · • · • ·

Generation of an inhomogeneous alternating magnetic field MW advantageously applicable coil known.

FIG. 10 shows a symmetrical trapezoidal profile of the coil drive signal SA applied to the coil S1 of the device 7 according to FIG. 5 for generating a trapezoid magnetic field pulse of the impressed magnetic alternating field MW. At a steep rising edge AF of the coil drive signal SA, a strongly increasing magnetic field of the alternating magnetic field MW is generated, which has a high ring current R in the nano-particle 1 result. Due to the subsequent plateau P of the coil drive signal SA, a magnetic field plateau is also formed in the nanoparticle 1, which causes a maximum oscillation deflection of the nano particle 1 by interaction of the ring current magnetic field with the magnetic field pulse. As a result of the induction of the ring current R, the temperature of the nano particle 1 and thus also its ohmic resistance increase, for which reason the falling edge AB and subsequent zero phase NP are used for the thermal cooling of the nano particle 1.

An extinction diode D of the device 7 connected in parallel with the coil S1 is designed to derive the energy stored in the coil S1 at the descent edges AB of the coil drive signal AS. FIG. 11 shows an asymmetrical trapezoidal profile of the coil drive signal SA applied to the coil S1 of the device 7, the descending edge AB being flatter than in FIG. With the flatter descent edge AB has the advantage that the electronics of the device 7 is spared and a longer life of the device 7 is obtained.

In some applications of the device 7, depending on the cells to be selected, the docking bodies used and other circumstances, it may be advantageous to impress a weak permanent magnetic field in addition to the magnetic field pulses of the alternating magnetic field MW. As a result, a weak ring current R is induced in the nano-particle 1, which is then amplified by the connection of the output from the coil 1 alternating magnetic field. This leads to a permanent pressure on the cell wall 3, which is amplified by the mechanical oscillations caused by the alternating magnetic field, which leads to the desired effect with greater efficiency. It should be noted that the induced by the weak permanent magnetic field in the nano-particle 1 ring current R does not heat the nano-particle 1 already in the initial situation too much. It is particularly advantageous if the signal modulator 9 is provided for outputting a coil drive signal SA * · · · · «+ ··· · t« * · ··· · ♦ ♦ · * · · · ··· · · · · containing a direct current component It is thus possible to provide for the provision of a separate permanent magnet in the... Device 7 can be dispensed with.

The device 7 now also has input means 11 with which the duty cycle of the output from the frequency generator 10 trapezoidal modulation signal MS or the output from the signal modulator 9 trapezoidal Spulenansteuerungssignals AS is adjustable. As a result, the alternating magnetic field MW generated by the device 7 can be adapted to the application, in this case also the input means 11 for setting the DC component of the coil drive signal AS is particularly advantageous.

Following a case of application of the device 7 according to the invention will now be explained in more detail. It is believed that biologists are interested in how fast certain bacteria X spread from a pond A (small pond) to a pond B only 100 meters away. This question can be essential, for example, in the spread of diseases or poisoning. Propagation can be done, for example, by animals migrating from pool A into pool B, such as birds or frogs carrying individual bacteria X from pool A into pool B.

For example, the bacterium X could be the water-borne bacterium cyanobacteria, also called blue-green algae. According to the example, blue-green algae are detectable both in the pool A and in the pool B, whereby it is to be verified whether and in what quantity and time unit blue-green algae pass from the pool A into the pool B. In order to answer this question, a docking body is fixed for the time being, which docks to the blue-green algae, but not to other bacteria in the ponds A and B. In the case of blue-green algae, there are inexpensive antibodies available on the market. These antibodies are now provided in a chemical process with nano-particles 1 and form a docking substance, which is introduced into the pool A. The antibodies selected for this application typically dock to blue-green algae within the pool within 24 hours. From this follows the starting point for the attempt that in the pool A are provided with nano-particles blue algae, where in the pool B Although blue-green algae, but not provided with nano-particles blue algae are included.

Taking, for example, after a week, a sample or an environmental medium from pool B and uses the device 7 according to the invention, such as

shown in Figure 6, for impressing the alternating magnetic field MW in the surrounding medium UM, then it can be relatively easily and inexpensively determined whether it has come to a transport of nano-particles blue-green algae from the pool A in the pool B. The blue-green algae die under the influence of the device 7 only if they are appropriately provided with nano-particles 1. After another week a sample can be taken again and so the temporal change of the nano-particles 1 having blue-green algae in the pool B are recorded.

By means of this simple example, the broad applicability of the invention will become apparent to those skilled in the art. Thus, the diffusion of nano-particle cells in subterranean streams or through masonry or organisms could also be determined. Another broad area of potential use cases arises in terms of destroying certain nanoparticle-containing cells to give other cells better growth opportunities. For example, a bacterium or some type of pest cell in potatoes could slow down or completely displace the growth of cells desired in the potato. Such potatoes could not be eaten then. According to the invention, a suitable docking substance could be introduced into the potato plant, for example by pouring the potatoes. After one day, the pest cells could then be destroyed by a single or multiple impressions of a suitable for this application magnetic alternating field in the still young potato plant. Another broad field of possible applications is in laboratory medicine or medicine itself and in other more remote disciplines.

It may be mentioned that it may be advantageous to provide a cooling device in the device 7 for cooling the coil S1, if the device 7 is provided for a continuous operation. An active cooling can be, for example, that the coil S1 is housed in a housing which is traversed by a coolant, so that the coil S1 is flowed around by this coolant. Alternatively, the wrapping material itself may also be hollow and flow through the coolant.

It can be mentioned that it is involved in the destruction of selected cells in organisms

It is advantageous to allow time to elapse between individual applications of the device 7, so that the organism can break down the dead cells and, as a result, poisoning does not occur in the organism.

It can be mentioned that at frequencies of the coil drive signal AS around the 100 Hz, a symmetrical duty cycle can be used, as shown in FIG. 10, whereby at higher frequencies the asymmetrical duty cycle shown in FIG. 11 can be advantageous. In particular, longer zero phase NP for cooling the nano-particles are advantageous in this case.

It may be mentioned that even a rectangular or sinusoidal coil drive signal may be advantageous in certain applications.

Claims (15)

·····································································. Verfahren 12776 Claims: 1. A method for rupturing cell walls (3) of selected cells (2), characterized in that in a first method step a docking substance of docking bodies (6) provided with electrically conductive nanoparticles (1) , which dock due to their properties only to certain cells (2), in a different cells exhibiting environment medium (UM) is introduced and wherein in a • second method step, the docking body (6) to this particular and thus selected cells (2 In the case of a third method step, an alternating magnetic field (MW) is impressed, which induces a ring current (R) in the nanoparticles (1) whose ring current magnetic field (RH1, RH2) interacts with the impressed alternating magnetic field (MW) and the nanoparticles (1) vibrates mechanically until the mechanical forces (F) acting on the cell walls (3) of the selected cells (2) thereby affect the cell fill up the walls (3). 2. The method according to claim 1, characterized in that in the third method step, an alternating magnetic field (MW) is impressed in the low frequency range in order to obtain maximum vibration deflection of the nano-particles (1) with minimal thermal heating of the nano-particles (1). 3. The method according to claim 2, characterized in that in the third method step, an alternating magnetic field (MW) is impressed with a modulable duty cycle to allow adaptation of the alternating magnetic field (MW) to different cell properties. 4. The method according to claim 3, characterized in that in the third method step, an alternating magnetic field (MW) is impressed with trapezoidal magnetic field pulses to • at a steep rising edge (AF) of the magnetic field pulse, a high ring current (R) in the nano-particles ( 1) to induce and at • a subsequent magnetic field plateau (P) of the magnetic field pulse by interaction of the ring current magnetic field (RH 1, RH2) with the impressed alternating magnetic field (MW) to cause maximum oscillation deflection of the nano-particles (1) and in order to «* • · • · · · · · · · · · · · · · · · · ··· «· · · · · • a subsequent descent (AB) and zero phase (NP) of the magnetic field pulse to cause a thermal cooling of the nano-particles (1). 5. The method according to claim 1, characterized in that the impressed in the third process step alternating magnetic field (MW) reaches a maximum flux density of at least 30 milli-Tessla. 6. The method according to claim 1, characterized in that the impressed in the third step, the alternating magnetic field (MW) is highly inhomogeneous. 7. The method according to claim 6, characterized in that in the third method step impressed magnetic alternating field (MW) by two coils (S2) is generated in the substantially simultaneously oppositely polarized drive signals (SA) for generating the alternating magnetic field (MW) be fed. 8. The method according to claim 1, characterized in that in the third method step in addition to the alternating magnetic field (MW) a weak permanent magnetic field is impressed. 9. The method according to claim 1, characterized in that antibodies are provided as docking body (6) for docking to certain properties having selected cells (2). 10. Device (7) for tearing open cell walls (3) of selected cells (2) for processing the third method step of the method according to one of claims 1 to 9 with a coil (S1) for generating an alternating magnetic field (MW) and with a current source (8) for outputting a direct current (I) and having a signal modulator (9) for modulating the direct current (I) with a modulation signal (MS) output from a frequency generator (10) and outputting a coil driving signal (SA) to the coil ( Sl) and one of the coil (Sl) in parallel with a quenching diode (D) for deriving the energy stored in the coil (Sl) at the descending edges (AB) of the modulation signal (MS). 11. Device (7) according to claim 10, characterized in that the frequency generator (10) for outputting a trapezoidal modulation signal (MS) is formed. •····························································· 12. Device (7) according to claim 11, characterized in that the input means (11) are provided, with which the duty cycle of the frequency generator (10) output trapezoidal modulation signal (MS) is adjustable. 13. Device (7) according to claim 10, characterized in that the signal modulator (9) for outputting a DC component containing coil drive signal (SA) is formed. 14. docking substance for use in a method according to any one of claims 1 to 9, characterized in that in the docking substance with electrically conductive nanoparticles (1) provided docking body (6), due to their properties only to dock certain cells (2) are provided, wherein the docking substance is introduced into a different cells having environmental medium (UM) and wherein the docking body (6) to this particular and thereby selected cells (2) docked in the Ambient medium (UM) are contained and wherein by impressing an alternating magnetic field (MW) in the ambient medium (UM), in the nano-particles (1) a ring current (R) is inducible whose ring current magnetic field (RH1, RH2) interacts with the impressed alternating magnetic field (MW) and the nano-particles (1) are displaceable in mechanical wings until the force acting on the cell walls (3) of the selected cells (2) mechanical forces (F) for tearing de R cell walls (3) are formed. 15. docking substance according to claim 14, characterized in that antibodies are provided as docking body (6) for docking to certain properties having selected cells (2).