AR133303A1 - Vectores que incorporan una combinación de promotores que impulsan la expresión de marcadores seleccionables - Google Patents
Vectores que incorporan una combinación de promotores que impulsan la expresión de marcadores seleccionablesInfo
- Publication number
- AR133303A1 AR133303A1 ARP240101890A ARP240101890A AR133303A1 AR 133303 A1 AR133303 A1 AR 133303A1 AR P240101890 A ARP240101890 A AR P240101890A AR P240101890 A ARP240101890 A AR P240101890A AR 133303 A1 AR133303 A1 AR 133303A1
- Authority
- AR
- Argentina
- Prior art keywords
- promoter
- chain
- operably linked
- strand
- expression
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
- C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
- C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
- C12N9/003—Dihydrofolate reductase [DHFR] (1.5.1.3)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
- C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
- C12Y603/01—Acid-ammonia (or amine)ligases (amide synthases)(6.3.1)
- C12Y603/01002—Glutamate-ammonia ligase (6.3.1.2)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Reproductive Health (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Se proporcionan sistemas de vectores duales, y células hospedadoras de mamíferos que los comprenden, para la expresión de moléculas multicadena en los que cada vector emplea un promotor diferente que impulsa la expresión de un marcador seleccionable. Las células hospedadoras de mamíferos pueden utilizarse para producir una proteína recombinante de interés en un cultivo de células de mamífero. Reivindicación 1: Una célula hospedadora de mamífero para expresar una proteína de unión a antígeno que tiene tres o cuatro cadenas diferentes que comprende un primer vector de expresión y un segundo vector de expresión, en el que: (a) el primer vector de expresión comprende secuencias de nucleótidos que codifican 1) una primera cadena y una segunda cadena, en las que la primera cadena está unida de manera operativa a un primer promotor, la segunda cadena está unida de manera operativa a un segundo promotor o a una secuencia IRES o está unida a la primera cadena a través de una secuencia enlazadora; y 2) un primer marcador seleccionable unido de manera operativa a un tercer promotor; y (b) el segundo vector de expresión comprende secuencias de nucleótidos que codifican 1) una tercera cadena y una cuarta cadena, en las que la tercera cadena está unida de manera operativa a un cuarto promotor, la cuarta cadena está unida de manera operativa a un quinto promotor o a una secuencia IRES o está unida a la tercera cadena a través de una secuencia enlazadora; y 2) un segundo marcador seleccionable unido de manera operativa a un sexto promotor, en la que la primera, segunda, tercera y cuarta cadenas se seleccionan entre el grupo que consiste en una cadena pesada, una cadena ligera, una fusión de cadena pesada de anticuerpo, una fusión de cadena ligera de anticuerpo, un ScFv y un ScFv-Fc, en el que dos de las cadenas pueden ser idénticas; y en el que el sexto promotor es diferente del tercer promotor.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202363528181P | 2023-07-21 | 2023-07-21 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR133303A1 true AR133303A1 (es) | 2025-09-17 |
Family
ID=92259033
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP240101890A AR133303A1 (es) | 2023-07-21 | 2024-07-19 | Vectores que incorporan una combinación de promotores que impulsan la expresión de marcadores seleccionables |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20250027052A1 (es) |
| CN (1) | CN121443633A (es) |
| AR (1) | AR133303A1 (es) |
| AU (1) | AU2024300966A1 (es) |
| TW (1) | TW202519647A (es) |
| WO (1) | WO2025024295A1 (es) |
Family Cites Families (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5672502A (en) | 1985-06-28 | 1997-09-30 | Celltech Therapeutics Limited | Animal cell culture |
| US4965195A (en) | 1987-10-26 | 1990-10-23 | Immunex Corp. | Interleukin-7 |
| US4968607A (en) | 1987-11-25 | 1990-11-06 | Immunex Corporation | Interleukin-1 receptors |
| WO1990005183A1 (en) | 1988-10-31 | 1990-05-17 | Immunex Corporation | Interleukin-4 receptors |
| WO1991018982A1 (en) | 1990-06-05 | 1991-12-12 | Immunex Corporation | Type ii interleukin-1 receptors |
| GB9014932D0 (en) | 1990-07-05 | 1990-08-22 | Celltech Ltd | Recombinant dna product and method |
| US6319494B1 (en) | 1990-12-14 | 2001-11-20 | Cell Genesys, Inc. | Chimeric chains for receptor-associated signal transduction pathways |
| WO1994004679A1 (en) | 1991-06-14 | 1994-03-03 | Genentech, Inc. | Method for making humanized antibodies |
| JPH05244982A (ja) | 1991-12-06 | 1993-09-24 | Sumitomo Chem Co Ltd | 擬人化ｂ−ｂ１０ |
| IL104570A0 (en) | 1992-03-18 | 1993-05-13 | Yeda Res & Dev | Chimeric genes and cells transformed therewith |
| WO1997025420A1 (en) | 1996-01-11 | 1997-07-17 | Immunex Corporation | Expression augmenting sequence elements (ease) for eukaryotic expression systems |
| CA2196496A1 (en) | 1997-01-31 | 1998-07-31 | Stephen William Watson Michnick | Protein fragment complementation assay for the detection of protein-protein interactions |
| GB9722131D0 (en) | 1997-10-20 | 1997-12-17 | Medical Res Council | Method |
| GB9928787D0 (en) | 1999-12-03 | 2000-02-02 | Medical Res Council | Direct screening method |
| US6544424B1 (en) | 1999-12-03 | 2003-04-08 | Refined Technology Company | Fluid filtration system |
| GB0025144D0 (en) | 2000-10-13 | 2000-11-29 | Medical Res Council | Concatenated nucleic acid sequences |
| US20040202995A1 (en) | 2003-04-09 | 2004-10-14 | Domantis | Nucleic acids, proteins, and screening methods |
| CN1938428A (zh) * | 2003-11-12 | 2007-03-28 | 先灵公司 | 多基因表达的质粒系统 |
| EP1614755A1 (en) | 2004-07-07 | 2006-01-11 | ARTEMIS Pharmaceuticals GmbH | Target transgenesis of short hairpin RNA expression cassette using recombinase mediated cassette exchange |
| US8053238B2 (en) | 2005-10-31 | 2011-11-08 | Unhwa Corporation | Isolated population of plant single cells and method of preparing the same |
| US20100221823A1 (en) | 2007-06-11 | 2010-09-02 | Amgen Inc. | Method for culturing mammalian cells to improve recombinant protein production |
| CA2718499C (en) * | 2008-03-14 | 2019-06-11 | Helene Haegel | Antibody against the csf-1r |
| KR20160015400A (ko) | 2008-08-22 | 2016-02-12 | 상가모 바이오사이언스 인코포레이티드 | 표적화된 단일가닥 분할 및 표적화된 통합을 위한 방법 및 조성물 |
| US9133493B2 (en) | 2011-04-21 | 2015-09-15 | Amgen Inc. | Method for culturing mammalian cells to improve recombinant protein production |
| US10202261B2 (en) | 2017-04-18 | 2019-02-12 | Kuwait University | Heuristic fuzzy controller for gantry cranes |
| KR20210101270A (ko) | 2018-12-10 | 2021-08-18 | 암젠 인크 | 돌연변이 피기백 트랜스포사제 |
| WO2020254351A1 (en) * | 2019-06-19 | 2020-12-24 | F. Hoffmann-La Roche Ag | Method for the generation of a multivalent, multispecific antibody expressing cell by targeted integration of multiple expression cassettes in a defined organization |
| CN115397853A (zh) | 2019-12-17 | 2022-11-25 | 辉瑞大药厂 | 对cd47、pd-l1具特异性的抗体及其用途 |
-
2024
- 2024-07-19 AR ARP240101890A patent/AR133303A1/es unknown
- 2024-07-19 TW TW113127034A patent/TW202519647A/zh unknown
- 2024-07-19 AU AU2024300966A patent/AU2024300966A1/en active Pending
- 2024-07-19 WO PCT/US2024/038797 patent/WO2025024295A1/en active Pending
- 2024-07-19 US US18/778,486 patent/US20250027052A1/en active Pending
- 2024-07-19 CN CN202480044826.XA patent/CN121443633A/zh active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20250027052A1 (en) | 2025-01-23 |
| AU2024300966A1 (en) | 2026-01-08 |
| TW202519647A (zh) | 2025-05-16 |
| WO2025024295A1 (en) | 2025-01-30 |
| CN121443633A (zh) | 2026-01-30 |
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