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AR132724A1 - DNA CONSTRUCTION ENCODING A FUSION PROTEIN WITH AT LEAST TWO ENTEROHEMORRHAGIC ESCHERICHIA COLI ANTIGENS, VECTORS, FUSION PROTEIN, IMMUNIZATION METHODS AND VACCINES - Google Patents

DNA CONSTRUCTION ENCODING A FUSION PROTEIN WITH AT LEAST TWO ENTEROHEMORRHAGIC ESCHERICHIA COLI ANTIGENS, VECTORS, FUSION PROTEIN, IMMUNIZATION METHODS AND VACCINES

Info

Publication number
AR132724A1
AR132724A1 ARP240101266A ARP240101266A AR132724A1 AR 132724 A1 AR132724 A1 AR 132724A1 AR P240101266 A ARP240101266 A AR P240101266A AR P240101266 A ARP240101266 A AR P240101266A AR 132724 A1 AR132724 A1 AR 132724A1
Authority
AR
Argentina
Prior art keywords
fusion protein
escherichia coli
antigens
vaccines
vectors
Prior art date
Application number
ARP240101266A
Other languages
Spanish (es)
Inventor
Angel Adrin Cataldi
Daniel Vilte
Mariano Larzabal
Original Assignee
Instituto Nac De Tecnologia Agropecuaria Inta
Consejo Nacional De Investigaciones Cientificas Y Tecn Conicet
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Instituto Nac De Tecnologia Agropecuaria Inta, Consejo Nacional De Investigaciones Cientificas Y Tecn Conicet filed Critical Instituto Nac De Tecnologia Agropecuaria Inta
Priority to ARP240101266A priority Critical patent/AR132724A1/en
Priority to PCT/ES2025/070282 priority patent/WO2025238284A1/en
Publication of AR132724A1 publication Critical patent/AR132724A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/025—Enterobacteriales, e.g. Enterobacter
    • A61K39/0258—Escherichia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/025—Enterobacteriales, e.g. Enterobacter
    • A61K39/0275—Salmonella
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/53—DNA (RNA) vaccination
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/55—Medicinal preparations containing antigens or antibodies characterised by the host/recipient, e.g. newborn with maternal antibodies
    • A61K2039/552—Veterinary vaccine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/70—Multivalent vaccine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Construcción de ADN que codifica una proteína de fusión que comprende al menos dos antígenos de Escherichia coli enterohemorrágica, por ejemplo, los antígenos Int280, EspB, EspA, Stx2B, EspD, combinaciones o fragmentos de los mismos, vectores, proteína de fusión, métodos de inmunización y vacunas. Los antígenos codificados pueden ser Int280 y EspB de Escherichia coli enterohemorrágica fusionados, formando una proteína de fusión con o sin una secuencia conectora entre ambos. Los antígenos fusionados pueden mostrar la secuencia de aminoácidos SEQ ID Nº 3 o SEQ ID Nº 4 y son codificados por la secuencia de nucleótidos mostrada en SEQ ID Nº 1 y SEQ ID Nº 2. La construcción puede además comprender una secuencia señal y una secuencia de anclaje a la membrana de las bacterias.DNA construct encoding a fusion protein comprising at least two enterohemorrhagic Escherichia coli antigens, for example, the Int280, EspB, EspA, Stx2B, EspD antigens, combinations or fragments thereof, vectors, fusion protein, immunization methods and vaccines. The encoded antigens can be Int280 and EspB from enterohemorrhagic Escherichia coli fused, forming a fusion protein with or without a linker sequence between them. The fused antigens can have the amino acid sequence SEQ ID No. 3 or SEQ ID No. 4 and are encoded by the nucleotide sequence shown in SEQ ID No. 1 and SEQ ID No. 2. The construct can also comprise a signal sequence and a sequence for anchoring to the bacterial membrane.

ARP240101266A 2024-05-17 2024-05-17 DNA CONSTRUCTION ENCODING A FUSION PROTEIN WITH AT LEAST TWO ENTEROHEMORRHAGIC ESCHERICHIA COLI ANTIGENS, VECTORS, FUSION PROTEIN, IMMUNIZATION METHODS AND VACCINES AR132724A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ARP240101266A AR132724A1 (en) 2024-05-17 2024-05-17 DNA CONSTRUCTION ENCODING A FUSION PROTEIN WITH AT LEAST TWO ENTEROHEMORRHAGIC ESCHERICHIA COLI ANTIGENS, VECTORS, FUSION PROTEIN, IMMUNIZATION METHODS AND VACCINES
PCT/ES2025/070282 WO2025238284A1 (en) 2024-05-17 2025-05-16 Dna construct that codes for a fusion protein with at least two enterohemorrhagic escherichia coli antigens, vectors, fusion protein, immunisation methods and vaccines

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ARP240101266A AR132724A1 (en) 2024-05-17 2024-05-17 DNA CONSTRUCTION ENCODING A FUSION PROTEIN WITH AT LEAST TWO ENTEROHEMORRHAGIC ESCHERICHIA COLI ANTIGENS, VECTORS, FUSION PROTEIN, IMMUNIZATION METHODS AND VACCINES

Publications (1)

Publication Number Publication Date
AR132724A1 true AR132724A1 (en) 2025-07-23

Family

ID=96230336

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP240101266A AR132724A1 (en) 2024-05-17 2024-05-17 DNA CONSTRUCTION ENCODING A FUSION PROTEIN WITH AT LEAST TWO ENTEROHEMORRHAGIC ESCHERICHIA COLI ANTIGENS, VECTORS, FUSION PROTEIN, IMMUNIZATION METHODS AND VACCINES

Country Status (2)

Country Link
AR (1) AR132724A1 (en)
WO (1) WO2025238284A1 (en)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6261561B1 (en) 1996-04-19 2001-07-17 Henry M. Jackson Foundation For The Advancement Of Military Medicine Method of stimulating an immune response by administration of host organisms that express intimin alone or as a fusion protein with one or more other antigens
WO2006047517A2 (en) 2004-10-26 2006-05-04 University Of Maryland Baltimore Live attenuated bacterial vaccine to reduce or inhibit carriage and shedding of enterohemorrhagic escherichia coli in cattle
WO2010115278A1 (en) 2009-04-06 2010-10-14 University Of Saskatchewan Methods and compositions for treating and preventing shiga toxin-producing escherichia coli infection
WO2013164334A1 (en) 2012-05-04 2013-11-07 Universiteit Gent Escherichia coli vaccine

Also Published As

Publication number Publication date
WO2025238284A1 (en) 2025-11-20

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