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AR132405A1 - Methods and systems for selecting biomarkers based on DNA methylation for the detection of colorectal cancer - Google Patents

Methods and systems for selecting biomarkers based on DNA methylation for the detection of colorectal cancer

Info

Publication number
AR132405A1
AR132405A1 ARP240100917A ARP240100917A AR132405A1 AR 132405 A1 AR132405 A1 AR 132405A1 AR P240100917 A ARP240100917 A AR P240100917A AR P240100917 A ARP240100917 A AR P240100917A AR 132405 A1 AR132405 A1 AR 132405A1
Authority
AR
Argentina
Prior art keywords
colorectal cancer
biomarkers
detection
methods
methylation
Prior art date
Application number
ARP240100917A
Other languages
Spanish (es)
Inventor
Emanuel Martin Campoy
Original Assignee
Consejo Nacional De Investigaciones Cientificas Y Tecn Conicet
Univ Nacional De Cuyo Uncuyo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Nacional De Investigaciones Cientificas Y Tecn Conicet, Univ Nacional De Cuyo Uncuyo filed Critical Consejo Nacional De Investigaciones Cientificas Y Tecn Conicet
Publication of AR132405A1 publication Critical patent/AR132405A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • G16B25/10—Gene or protein expression profiling; Expression-ratio estimation or normalisation
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16H—HEALTHCARE INFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR THE HANDLING OR PROCESSING OF MEDICAL OR HEALTHCARE DATA
    • G16H50/00—ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics
    • G16H50/20—ICT specially adapted for medical diagnosis, medical simulation or medical data mining; ICT specially adapted for detecting, monitoring or modelling epidemics or pandemics for computer-aided diagnosis, e.g. based on medical expert systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medical Informatics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Evolutionary Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Theoretical Computer Science (AREA)
  • Public Health (AREA)
  • Data Mining & Analysis (AREA)
  • Databases & Information Systems (AREA)
  • Epidemiology (AREA)
  • Primary Health Care (AREA)
  • Medicinal Chemistry (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere a herramientas bioinformáticas para identificar biomarcadores basados en metilación de ADN y métodos para detectar estos biomarcadores en muestras de sangre de pacientes con cáncer colorrectal (CCR). Por lo tanto, la presente invención proporciona un algoritmo para seleccionar biomarcadores de metilación y un sistema de hemi-sonda para la detección de múltiples biomarcadores de metilación en biopsias líquidas de pacientes con CCR utilizando amplificación basada en enzimas de restricción. La presente invención describe además un panel de detección de cáncer colorrectal que comprende sitios CpG de 15 biomarcadores en base a metilación.The present invention relates to bioinformatics tools for identifying DNA methylation-based biomarkers and methods for detecting these biomarkers in blood samples from patients with colorectal cancer (CRC). Therefore, the present invention provides an algorithm for selecting methylation biomarkers and a half-probe system for the detection of multiple methylation biomarkers in liquid biopsies from patients with CRC using restriction enzyme-based amplification. The present invention further describes a colorectal cancer screening panel comprising 15 CpG sites of methylation-based biomarkers.

ARP240100917A 2023-04-19 2024-04-11 Methods and systems for selecting biomarkers based on DNA methylation for the detection of colorectal cancer AR132405A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US202363460335P 2023-04-19 2023-04-19

Publications (1)

Publication Number Publication Date
AR132405A1 true AR132405A1 (en) 2025-06-25

Family

ID=93153160

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP240100917A AR132405A1 (en) 2023-04-19 2024-04-11 Methods and systems for selecting biomarkers based on DNA methylation for the detection of colorectal cancer

Country Status (2)

Country Link
AR (1) AR132405A1 (en)
WO (1) WO2024220595A1 (en)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070092883A1 (en) * 2005-10-26 2007-04-26 De Luwe Hoek Octrooien B.V. Methylation specific multiplex ligation-dependent probe amplification (MS-MLPA)
EP3512959B1 (en) * 2016-09-15 2021-08-11 Roche Diagnostics GmbH Methods for performing multiplexed pcr
US12480164B2 (en) * 2018-12-04 2025-11-25 EpiMedTechGlobal (EMTG) DNA methylation biomarkers for early detection of cervical cancer
CN116064815B (en) * 2019-02-14 2024-12-03 博尔诚（北京）科技有限公司 Esophageal cancer, gastric cancer, intestinal cancer, liver cancer and/or pancreatic cancer pre-cancer screening composition and application thereof
CN112779334B (en) * 2021-02-01 2022-05-27 杭州医学院 Methylation marker combination for early screening of prostate cancer and screening method

Also Published As

Publication number Publication date
WO2024220595A1 (en) 2024-10-24

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