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AR132218A1 - Enzimas pesticidas para uso en nematodos, insectos y moluscos - Google Patents

Enzimas pesticidas para uso en nematodos, insectos y moluscos

Info

Publication number
AR132218A1
AR132218A1 ARP240100727A ARP240100727A AR132218A1 AR 132218 A1 AR132218 A1 AR 132218A1 AR P240100727 A ARP240100727 A AR P240100727A AR P240100727 A ARP240100727 A AR P240100727A AR 132218 A1 AR132218 A1 AR 132218A1
Authority
AR
Argentina
Prior art keywords
sequence
seq
nos
enzyme
plant
Prior art date
Application number
ARP240100727A
Other languages
English (en)
Inventor
Brian M Thompson
Andrew M Mutka
Alejandro Tovar-Mendez
Erica D Mueller
Original Assignee
Spogen Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Spogen Biotech Inc filed Critical Spogen Biotech Inc
Publication of AR132218A1 publication Critical patent/AR132218A1/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00—Plant growth regulators
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P5/00—Nematocides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00—Arthropodicides
    • A01P7/04—Insecticides
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P9/00—Molluscicides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2442—Chitinase (3.2.1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Insects & Arthropods (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Se proporcionan enzimas aisladas que exhiben actividad insecticida, nematicida o pesticida, y microorganismos recombinantes que expresan dichas enzimas, así como métodos para usar las mismas para proteger una planta de un patógeno o plaga, mejorar la salud de la planta o mejorar el crecimiento de la planta. Reivindicación 1: Una composición para proteger una planta de una plaga o patógeno que comprende por lo menos una enzima aislada seleccionada entre una esterasa, una quitinasa, una proteasa, una lipasa, y combinaciones de cualquiera de las mismas; en donde: a) la quitinasa comprende una secuencia que tiene por lo menos 85% de identidad de secuencia con una secuencia seleccionada entre las SEQ ID Nº 179 - 205, y 249; b) la proteasa comprende una secuencia que tiene por lo menos 85% de identidad de secuencia con una secuencia seleccionada entre las SEQ ID Nº 122 - 178, 247, y 310 - 327; o c) la lipasa comprende una secuencia que tiene por lo menos 85% de identidad de secuencia con una secuencia seleccionada entre las SEQ ID Nº 1 - 82, 250, 251, 256 - 260, y 290 - 307; y en donde la composición exhibe actividad insecticida, nematicida o pesticida. Reivindicación 4: Una semilla de planta recubierta con la composición de la reivindicación 1. Reivindicación 16: Un microorganismo recombinante que expresa una enzima, en donde la expresión de la enzima aumenta en comparación con el nivel de expresión de la enzima en un microorganismo de tipo salvaje del mismo tipo en las mismas condiciones; y la enzima se selecciona entre una esterasa, una quitinasa, una proteasa, una lipasa, y combinaciones de cualquiera de las mismas; y en donde: a) la quitinasa comprende una secuencia que tiene por lo menos 85% de identidad de secuencia con una secuencia seleccionada entre las SEQ ID Nº 179 - 205, y 249; b) la proteasa comprende una secuencia que tiene por lo menos 85% de identidad de secuencia con una secuencia seleccionada entre las SEQ ID Nº 122 - 178, 247, y 310 - 327; o c) la lipasa comprende una secuencia que tiene por lo menos 85% de identidad de secuencia con una secuencia seleccionada entre las SEQ ID Nº 1 - 82, 250, 251, 256 - 260, y 290 - 307; y en donde el microorganismo recombinante exhibe actividad insecticida, nematicida o pesticida. Reivindicación 17: Una composición para proteger una planta de una plaga o patógeno, que comprende el microorganismo recombinante de la reivindicación 16. Reivindicación 22: Un producto de fermentación del microorganismo recombinante de la reivindicación 16. Reivindicación 23: Una formulación que comprende el producto de fermentación de la reivindicación 22 y al menos un portador agrícolamente aceptable.
ARP240100727A 2023-03-28 2024-03-26 Enzimas pesticidas para uso en nematodos, insectos y moluscos AR132218A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US202363492672P 2023-03-28 2023-03-28

Publications (1)

Publication Number Publication Date
AR132218A1 true AR132218A1 (es) 2025-06-04

Family

ID=92899391

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP240100727A AR132218A1 (es) 2023-03-28 2024-03-26 Enzimas pesticidas para uso en nematodos, insectos y moluscos

Country Status (5)

Country Link
US (1) US20240324599A1 (es)
AR (1) AR132218A1 (es)
AU (1) AU2024249083A1 (es)
MX (1) MX2025011515A (es)
WO (1) WO2024206229A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112018068739B1 (pt) 2016-03-16 2023-10-03 Spogen Biotech Inc Métodos para estimular o crescimento de plantas e/ou promover a fitossanidade, semente de planta revestida e composição

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150139976A1 (en) * 2011-12-28 2015-05-21 Council Of Scientific & Industrial Research Novel insecticidal chitinase protein its encoding nucleotide and application thereof
BR112018068739B1 (pt) * 2016-03-16 2023-10-03 Spogen Biotech Inc Métodos para estimular o crescimento de plantas e/ou promover a fitossanidade, semente de planta revestida e composição
CN106399335B (zh) * 2016-10-19 2019-05-21 大连理工大学 高效几丁质酶突变体的制备方法及应用
US20210251238A1 (en) * 2018-06-05 2021-08-19 Novozymes Bioag A/S Methods of protecting a plant from insect pests

Also Published As

Publication number Publication date
WO2024206229A1 (en) 2024-10-03
MX2025011515A (es) 2025-11-03
AU2024249083A1 (en) 2025-09-25
US20240324599A1 (en) 2024-10-03

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