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AR132127A1 - NUCLEIC ACID SEQUENCE FOR THE DETECTION OF THE CORN PLANT DBN9235 AND METHOD FOR DETECTING SAME - Google Patents

NUCLEIC ACID SEQUENCE FOR THE DETECTION OF THE CORN PLANT DBN9235 AND METHOD FOR DETECTING SAME

Info

Publication number
AR132127A1
AR132127A1 ARP240100623A ARP240100623A AR132127A1 AR 132127 A1 AR132127 A1 AR 132127A1 AR P240100623 A ARP240100623 A AR P240100623A AR P240100623 A ARP240100623 A AR P240100623A AR 132127 A1 AR132127 A1 AR 132127A1
Authority
AR
Argentina
Prior art keywords
dbn9235
nucleic acid
acid sequence
corn plant
detection
Prior art date
Application number
ARP240100623A
Other languages
Spanish (es)
Inventor
Lijun Wang
Haili Liu
Ming Zhang
Mengmeng Tao
Caihong Yu
Cheng Wang
Original Assignee
Beijing Dabeinong Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beijing Dabeinong Biotechnology Co Ltd filed Critical Beijing Dabeinong Biotechnology Co Ltd
Publication of AR132127A1 publication Critical patent/AR132127A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • A01H1/045—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection using molecular markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8277—Phosphinotricin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Environmental Sciences (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Developmental Biology & Embryology (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere a una secuencia de ácido nucleico para detectar la planta de maíz DBN9235 y a un método de detección de la misma. La secuencia de ácido nucleico incluye SEQ ID Nº 1 o secuencia complementaria de la misma, o SEQ ID Nº 2 o secuencia complementaria de la misma. La planta de maíz DBN9235 de la presente invención tiene buena resistencia a los insectos lepidópteros y buena tolerancia a los herbicidas glufosinato, no tiene impacto en el rendimiento, y el método de detección puede identificar con precisión y rapidez si las muestras biológicas contienen una molécula de ADN del evento de maíz transgénico DBN9235.The present invention relates to a nucleic acid sequence for detecting the DBN9235 corn plant and a method for detecting the same. The nucleic acid sequence includes SEQ ID No. 1 or a complementary sequence thereof, or SEQ ID No. 2 or a complementary sequence thereof. The DBN9235 corn plant of the present invention has good resistance to lepidopteran insects and good tolerance to the herbicide glufosinate, has no impact on yield, and the detection method can accurately and rapidly identify whether biological samples contain a DNA molecule of the transgenic corn event DBN9235.

ARP240100623A 2023-03-13 2024-03-13 NUCLEIC ACID SEQUENCE FOR THE DETECTION OF THE CORN PLANT DBN9235 AND METHOD FOR DETECTING SAME AR132127A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202310235129.XA CN116219063A (en) 2023-03-13 2023-03-13 Nucleic acid sequence and detection method for detecting corn plant DBN9235

Publications (1)

Publication Number Publication Date
AR132127A1 true AR132127A1 (en) 2025-05-28

Family

ID=86584227

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP240100623A AR132127A1 (en) 2023-03-13 2024-03-13 NUCLEIC ACID SEQUENCE FOR THE DETECTION OF THE CORN PLANT DBN9235 AND METHOD FOR DETECTING SAME

Country Status (3)

Country Link
CN (1) CN116219063A (en)
AR (1) AR132127A1 (en)
WO (1) WO2024188231A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116219063A (en) * 2023-03-13 2023-06-06 北京大北农生物技术有限公司 Nucleic acid sequence and detection method for detecting corn plant DBN9235
CN119530421A (en) * 2023-08-28 2025-02-28 青岛清原种子科学有限公司 Transgenic corn event QY2569-42 and its detection method

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104878090B (en) * 2015-04-30 2019-01-15 北京大北农科技集团股份有限公司 For detecting the nucleic acid sequence and its detection method of herbicide tolerant corn plant DBN9877
CA3066917A1 (en) * 2017-06-15 2018-12-20 Syngenta Crop Protection Ag Corn elite event mzir098
US12213421B2 (en) * 2019-08-09 2025-02-04 Beijing Dabeinong Biotechnology Co. Ltd. Nucleic acid sequence for detecting soybean plant DBN8002 and detection method therefor
CN111247255B (en) * 2019-08-09 2023-06-27 北京大北农生物技术有限公司 Nucleic acid sequence for detecting soybean plant DBN8007 and detection method thereof
CN113201531B (en) * 2021-04-27 2023-06-27 隆平生物技术(海南)有限公司 Transgenic corn event LW2-1 and detection method thereof
CN116219063A (en) * 2023-03-13 2023-06-06 北京大北农生物技术有限公司 Nucleic acid sequence and detection method for detecting corn plant DBN9235

Also Published As

Publication number Publication date
WO2024188231A1 (en) 2024-09-19
CN116219063A (en) 2023-06-06

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