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AR120690A1 - Daao modificada y uso de la misma - Google Patents

Daao modificada y uso de la misma

Info

Publication number
AR120690A1
AR120690A1 ARP200103406A ARP200103406A AR120690A1 AR 120690 A1 AR120690 A1 AR 120690A1 AR P200103406 A ARP200103406 A AR P200103406A AR P200103406 A ARP200103406 A AR P200103406A AR 120690 A1 AR120690 A1 AR 120690A1
Authority
AR
Argentina
Prior art keywords
daao
modified
present
glufosinate
same
Prior art date
Application number
ARP200103406A
Other languages
English (en)
Inventor
Xinkai Xie
Wei Xu
Junying Fan
Original Assignee
Sichuan Lier Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sichuan Lier Biotechnology Co Ltd filed Critical Sichuan Lier Biotechnology Co Ltd
Publication of AR120690A1 publication Critical patent/AR120690A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0022—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
    • C12N9/0024—D-Amino acid oxidase (1.4.3.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/002—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by oxidation/reduction reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/006—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by reactions involving C-N bonds, e.g. nitriles, amides, hydantoins, carbamates, lactames, transamination reactions, or keto group formation from racemic mixtures
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/50—Methods for regulating/modulating their activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/03—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
    • C12Y104/03003—D-Amino-acid oxidase (1.4.3.3)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente se refiere a una D-aminoácido oxidasa (DAAO) modificada. En particular, la DAAO modificada de la presente tiene actividad de catálisis de la oxidación de D-glufosinato en PPO. Además, la DAAO modificada de la presente tiene actividad aumentada de catálisis de la oxidación de D-glufosinato en PPO y/o estabilidad aumentada en comparación con la SEQ ID Nº 4. La presente también se refiere al polinucleótido que codifica la DAAO de la presente, al vector y célula hospedadora que expresa la DAAO modificada de la presente, y al método de producción de L-glufosinato con la DAAO modificada y la célula hospedadora de la presente.
ARP200103406A 2019-12-09 2020-12-09 Daao modificada y uso de la misma AR120690A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201911249924 2019-12-09

Publications (1)

Publication Number Publication Date
AR120690A1 true AR120690A1 (es) 2022-03-09

Family

ID=76328838

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP200103406A AR120690A1 (es) 2019-12-09 2020-12-09 Daao modificada y uso de la misma

Country Status (14)

Country Link
US (2) US11667896B2 (es)
EP (1) EP4074837A4 (es)
JP (1) JP7549017B2 (es)
KR (1) KR20220136350A (es)
CN (2) CN116286699A (es)
AR (1) AR120690A1 (es)
AU (1) AU2020401155B2 (es)
BR (1) BR112022010266A2 (es)
CA (1) CA3160673A1 (es)
IL (1) IL293679A (es)
MX (1) MX2022006534A (es)
TW (1) TWI777324B (es)
WO (1) WO2021115256A1 (es)
ZA (1) ZA202205945B (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20220136350A (ko) 2019-12-09 2022-10-07 후난 라이어 바이오테크날러지 씨오., 엘티디. 변형된 daao 효소 및 이의 적용
CN113881582A (zh) * 2021-11-11 2022-01-04 合肥工业大学 一株去除重金属离子的红酵母mf4、菌剂及其应用
CN116555206A (zh) * 2022-01-30 2023-08-08 弈柯莱生物科技(上海)股份有限公司 一种d-氨基酸氧化酶及其在制备l-草铵膦或其中间体中的应用
CN116064446B (zh) * 2022-12-02 2023-11-14 湖南麦肯伟科技有限公司 一种d-氨基酸氧化酶的突变体、表达载体、基因工程菌及其构建方法和应用
CN116200356A (zh) * 2022-12-05 2023-06-02 浙江工业大学 D-氨基酸氧化酶突变体及其在制备l-草铵膦中的应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60335938D1 (de) * 2002-02-26 2011-03-17 Syngenta Ltd Verfahren zur selektiven herstellung männlich- oder weiblich-steriler pflanzen
CN107636155B (zh) * 2015-04-03 2022-01-07 淡马锡生命科学研究院有限公司 红冬孢酵母属和红酵母属的d-氨基酸诱导型基因表达系统
CN105567780A (zh) * 2016-01-14 2016-05-11 重庆惠健生物科技有限公司 一种l-草铵膦的酶-化学催化去消旋化制备方法
KR20250156185A (ko) * 2016-03-02 2025-10-31 바스프 에스이 L-글루포시네이트를 제조하는 방법
CN107502647B (zh) 2017-09-15 2020-12-15 浙江大学 一种生物酶法去消旋化制备l-草铵膦的方法
CN108690854B (zh) 2018-04-16 2022-03-18 浙江工业大学 一种利用化学-酶法生产l-草铵膦的方法
US20210214754A1 (en) * 2018-09-05 2021-07-15 Basf Se Methods for improving yields of l-glufosinate
CN111019916B (zh) * 2018-11-23 2020-12-08 弈柯莱生物科技(上海)股份有限公司 一种d-氨基酸氧化酶突变体及其应用
CN109576236B (zh) * 2018-12-28 2019-12-17 浙江工业大学 一种d-氨基酸氧化酶突变体及其应用
CN109609582B (zh) 2019-01-17 2021-11-09 重庆惠健生物科技有限公司 一种微生物催化去消旋化制备l-草铵膦的方法
KR20220136350A (ko) 2019-12-09 2022-10-07 후난 라이어 바이오테크날러지 씨오., 엘티디. 변형된 daao 효소 및 이의 적용

Also Published As

Publication number Publication date
AU2020401155A1 (en) 2022-06-30
BR112022010266A2 (pt) 2022-10-04
EP4074837A4 (en) 2023-12-27
KR20220136350A (ko) 2022-10-07
JP2023504869A (ja) 2023-02-07
JP7549017B2 (ja) 2024-09-10
US20230416696A1 (en) 2023-12-28
EP4074837A1 (en) 2022-10-19
TW202128992A (zh) 2021-08-01
US20220333086A1 (en) 2022-10-20
US11667896B2 (en) 2023-06-06
WO2021115256A1 (zh) 2021-06-17
CA3160673A1 (en) 2021-06-17
CN116286699A (zh) 2023-06-23
US12180514B2 (en) 2024-12-31
CN113825841A (zh) 2021-12-21
ZA202205945B (en) 2025-08-27
IL293679A (en) 2022-08-01
TWI777324B (zh) 2022-09-11
MX2022006534A (es) 2022-09-07
AU2020401155B2 (en) 2024-10-31
CN113825841B (zh) 2022-08-09

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