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AR127148A1 - COMPOSITIONS AND METHODS FOR THE TRANSFORMATION OF EXPLANTS OF EMBRYOS REMOVED FROM MONOCOTYLEDON SEEDS - Google Patents

COMPOSITIONS AND METHODS FOR THE TRANSFORMATION OF EXPLANTS OF EMBRYOS REMOVED FROM MONOCOTYLEDON SEEDS

Info

Publication number
AR127148A1
AR127148A1 ARP220102581A ARP220102581A AR127148A1 AR 127148 A1 AR127148 A1 AR 127148A1 AR P220102581 A ARP220102581 A AR P220102581A AR P220102581 A ARP220102581 A AR P220102581A AR 127148 A1 AR127148 A1 AR 127148A1
Authority
AR
Argentina
Prior art keywords
methods
explant
transformation
compositions
explants
Prior art date
Application number
ARP220102581A
Other languages
Spanish (es)
Inventor
Justin Arsenault
Yurong Chen
Byung Kang
- Martinell Brian J Guk
Shubha Subbarao
Edward J Williams
Xudong Ye
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of AR127148A1 publication Critical patent/AR127148A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/743—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Agrobacterium; Rhizobium; Bradyrhizobium
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La invención presenta nuevas composiciones y métodos para la transformación o modificación asistida por fuerza de un explante embrionario de semilla de monocotiledónea. Los métodos proporcionados en el presente documento pueden incluir la aplicación de un tratamiento de presión y/o un tratamiento de fuerza gravitacional a un explante embrionario de semilla de monocotiledónea y la transformación de al menos una célula vegetal del explante embrionario con un polinucleótido heterólogo mediante la inoculación del explante embrionario con una bacteria Rhizobiales que comprende el polinucleótido heterólogo. Los métodos que se brindan en el presente documento también incluyen métodos de regeneración de una planta o parte de una planta modificada genéticamente a partir de una célula o explante vegetal transformado o editado.The invention presents new compositions and methods for the force-assisted transformation or modification of a monocot seed embryonic explant. The methods provided herein may include applying a pressure treatment and/or a gravitational force treatment to a monocot seed embryonic explant and transforming at least one plant cell of the embryonic explant with a heterologous polynucleotide by inoculation of the embryonic explant with a Rhizobiales bacteria comprising the heterologous polynucleotide. The methods provided herein also include methods of regeneration of a genetically modified plant or plant part from a transformed or edited plant cell or explant.

ARP220102581A 2021-09-27 2022-09-26 COMPOSITIONS AND METHODS FOR THE TRANSFORMATION OF EXPLANTS OF EMBRYOS REMOVED FROM MONOCOTYLEDON SEEDS AR127148A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US202163248945P 2021-09-27 2021-09-27

Publications (1)

Publication Number Publication Date
AR127148A1 true AR127148A1 (en) 2023-12-20

Family

ID=85721319

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP220102581A AR127148A1 (en) 2021-09-27 2022-09-26 COMPOSITIONS AND METHODS FOR THE TRANSFORMATION OF EXPLANTS OF EMBRYOS REMOVED FROM MONOCOTYLEDON SEEDS

Country Status (2)

Country Link
AR (1) AR127148A1 (en)
WO (1) WO2023049894A1 (en)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8044260B2 (en) * 2007-03-09 2011-10-25 Monsanto Technology Llc Method of meristem excision and transformation
AR116016A1 (en) * 2018-08-24 2021-03-25 Flagship Pioneering Innovations Vi Llc METHODS FOR MANUFACTURING VEGETABLE MESSENGER PACKAGES
US12022787B2 (en) * 2019-06-19 2024-07-02 Wisconsin Alumni Research Foundation Efficient monocot embryo extraction and preservation methods and novel uses thereof

Also Published As

Publication number Publication date
WO2023049894A1 (en) 2023-03-30

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