AR127022A1 - Adaptación de hospedadores de plataforma a medios igf - Google Patents
Adaptación de hospedadores de plataforma a medios igfInfo
- Publication number
- AR127022A1 AR127022A1 ARP220102449A ARP220102449A AR127022A1 AR 127022 A1 AR127022 A1 AR 127022A1 AR P220102449 A ARP220102449 A AR P220102449A AR P220102449 A ARP220102449 A AR P220102449A AR 127022 A1 AR127022 A1 AR 127022A1
- Authority
- AR
- Argentina
- Prior art keywords
- igf
- interest
- protein
- cell
- mammalian
- Prior art date
Links
- 230000006978 adaptation Effects 0.000 title 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
- C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/105—Insulin-like growth factors [IGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Reproductive Health (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
Se proporcionan métodos para producir una proteína recombinante de interés en un cultivo de células de mamífero en medios que carecen de IGF-1. También se proporcionan métodos para producir células de mamífero capaces de crecer en medios que carecen de IGF-1. Reivindicación 1: Un método para producir una proteína de interés a partir de un cultivo de células de mamífero que comprende: (a) cultivar una célula de mamífero que expresa una proteína de interés en un segundo medio de cultivo celular que tiene 0.05 mg/l o menos de factor de crecimiento insulínico (IGF-1) para expresar la proteína de interés, en donde la célula de mamífero se ha adaptado directamente a crecer en un primer medio de cultivo celular que tiene 0.03 mg/l o menos de IGF-1 y comprende un ácido nucleico heterólogo que codifica la proteína de interés; y (b) recuperar la proteína de interés producida por la célula de mamífero. Reivindicación 16: Un método para adaptar directamente una célula de mamífero a medios IGF⁻ que comprende: a) cultivar una población de células de mamífero en un medio de cultivo celular que comprende 0.03 mg/l o menos de IGF-1; b) obtener células individuales de la población de células de mamífero mediante clonación de una sola célula; c) expandir y someter a pases las células individuales hasta conseguir una recuperación del 90% o más y un tiempo de duplicación inferior a 30 horas.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163242623P | 2021-09-10 | 2021-09-10 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR127022A1 true AR127022A1 (es) | 2023-12-13 |
Family
ID=84053053
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP220102449A AR127022A1 (es) | 2021-09-10 | 2022-09-09 | Adaptación de hospedadores de plataforma a medios igf |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US20230082811A1 (es) |
| EP (1) | EP4399225A1 (es) |
| JP (1) | JP2024533319A (es) |
| KR (1) | KR20240054986A (es) |
| CN (1) | CN117897401A (es) |
| AR (1) | AR127022A1 (es) |
| AU (1) | AU2022344262A1 (es) |
| CA (1) | CA3230418A1 (es) |
| CL (1) | CL2024000698A1 (es) |
| IL (1) | IL310660A (es) |
| MX (1) | MX2024002959A (es) |
| TW (1) | TW202328442A (es) |
| WO (1) | WO2023039502A1 (es) |
Family Cites Families (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5672502A (en) | 1985-06-28 | 1997-09-30 | Celltech Therapeutics Limited | Animal cell culture |
| US4965195A (en) | 1987-10-26 | 1990-10-23 | Immunex Corp. | Interleukin-7 |
| US4968607A (en) | 1987-11-25 | 1990-11-06 | Immunex Corporation | Interleukin-1 receptors |
| AU643427B2 (en) | 1988-10-31 | 1993-11-18 | Immunex Corporation | Interleukin-4 receptors |
| WO1991018982A1 (en) | 1990-06-05 | 1991-12-12 | Immunex Corporation | Type ii interleukin-1 receptors |
| GB9014932D0 (en) | 1990-07-05 | 1990-08-22 | Celltech Ltd | Recombinant dna product and method |
| US6319494B1 (en) | 1990-12-14 | 2001-11-20 | Cell Genesys, Inc. | Chimeric chains for receptor-associated signal transduction pathways |
| WO1994004679A1 (en) | 1991-06-14 | 1994-03-03 | Genentech, Inc. | Method for making humanized antibodies |
| JPH05244982A (ja) | 1991-12-06 | 1993-09-24 | Sumitomo Chem Co Ltd | 擬人化ｂ−ｂ１０ |
| US5262308A (en) * | 1992-01-28 | 1993-11-16 | Thomas Jefferson University | Cell lines which constitutively express IGF-1 and IGF-1 R |
| IL104570A0 (en) | 1992-03-18 | 1993-05-13 | Yeda Res & Dev | Chimeric genes and cells transformed therewith |
| KR19990077015A (ko) | 1996-01-11 | 1999-10-25 | 크리스토퍼 엘. 와이트, 스코트 지. 홀퀴스트, 스티븐 엘. 말라스카 | 진핵 세포 발현 시스템을 위한 발현 증강 서열 요소 (ease) |
| CA2196496A1 (en) | 1997-01-31 | 1998-07-31 | Stephen William Watson Michnick | Protein fragment complementation assay for the detection of protein-protein interactions |
| GB9722131D0 (en) | 1997-10-20 | 1997-12-17 | Medical Res Council | Method |
| GB9928787D0 (en) | 1999-12-03 | 2000-02-02 | Medical Res Council | Direct screening method |
| US6544424B1 (en) | 1999-12-03 | 2003-04-08 | Refined Technology Company | Fluid filtration system |
| GB0025144D0 (en) | 2000-10-13 | 2000-11-29 | Medical Res Council | Concatenated nucleic acid sequences |
| US20040202995A1 (en) | 2003-04-09 | 2004-10-14 | Domantis | Nucleic acids, proteins, and screening methods |
| EP1614755A1 (en) | 2004-07-07 | 2006-01-11 | ARTEMIS Pharmaceuticals GmbH | Target transgenesis of short hairpin RNA expression cassette using recombinase mediated cassette exchange |
| US8053238B2 (en) | 2005-10-31 | 2011-11-08 | Unhwa Corporation | Isolated population of plant single cells and method of preparing the same |
| US20070231895A1 (en) * | 2005-11-02 | 2007-10-04 | Lee Gene W | Methods for adapting mammalian cells |
| CN103276033A (zh) * | 2006-09-13 | 2013-09-04 | 雅培制药有限公司 | 细胞培养改良 |
| WO2008154014A2 (en) | 2007-06-11 | 2008-12-18 | Amgen Inc. | A method for culturing mammalian cells to improve recombinant protein production |
| AU2009283194B2 (en) | 2008-08-22 | 2014-10-16 | Sangamo Therapeutics, Inc. | Methods and compositions for targeted single-stranded cleavage and targeted integration |
| US9133493B2 (en) | 2011-04-21 | 2015-09-15 | Amgen Inc. | Method for culturing mammalian cells to improve recombinant protein production |
| JP6195191B2 (ja) | 2013-08-08 | 2017-09-13 | 極東製薬工業株式会社 | 無タンパク質・無脂質培地馴化細胞株を用いた組換えタンパク質の製造方法 |
| EP3666035B1 (en) | 2017-08-07 | 2023-04-05 | LG Electronics Inc. | Method and apparatus for keeping dc configuration |
| EP3441471A1 (en) * | 2017-08-08 | 2019-02-13 | CEVEC Pharmaceuticals GmbH | Use of constitutively active variants of growth factor receptors as selection makers for the generation of stable producer cell lines |
-
2022
- 2022-09-08 TW TW111134166A patent/TW202328442A/zh unknown
- 2022-09-09 AR ARP220102449A patent/AR127022A1/es unknown
- 2022-09-09 AU AU2022344262A patent/AU2022344262A1/en active Pending
- 2022-09-09 US US17/930,810 patent/US20230082811A1/en active Pending
- 2022-09-09 CA CA3230418A patent/CA3230418A1/en active Pending
- 2022-09-09 WO PCT/US2022/076158 patent/WO2023039502A1/en not_active Ceased
- 2022-09-09 EP EP22800033.7A patent/EP4399225A1/en active Pending
- 2022-09-09 IL IL310660A patent/IL310660A/en unknown
- 2022-09-09 MX MX2024002959A patent/MX2024002959A/es unknown
- 2022-09-09 JP JP2024515076A patent/JP2024533319A/ja active Pending
- 2022-09-09 CN CN202280059449.8A patent/CN117897401A/zh active Pending
- 2022-09-09 KR KR1020247007110A patent/KR20240054986A/ko active Pending
-
2024
- 2024-03-08 CL CL2024000698A patent/CL2024000698A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CL2024000698A1 (es) | 2024-09-06 |
| AU2022344262A1 (en) | 2024-02-22 |
| CN117897401A (zh) | 2024-04-16 |
| KR20240054986A (ko) | 2024-04-26 |
| TW202328442A (zh) | 2023-07-16 |
| IL310660A (en) | 2024-04-01 |
| EP4399225A1 (en) | 2024-07-17 |
| MX2024002959A (es) | 2024-03-27 |
| JP2024533319A (ja) | 2024-09-12 |
| CA3230418A1 (en) | 2023-03-16 |
| US20230082811A1 (en) | 2023-03-16 |
| WO2023039502A1 (en) | 2023-03-16 |
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