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AR124881A2 - Métodos y composiciones para calidad de forraje mejorada - Google Patents

Métodos y composiciones para calidad de forraje mejorada

Info

Publication number
AR124881A2
AR124881A2 ARP220100303A ARP220100303A AR124881A2 AR 124881 A2 AR124881 A2 AR 124881A2 AR P220100303 A ARP220100303 A AR P220100303A AR P220100303 A ARP220100303 A AR P220100303A AR 124881 A2 AR124881 A2 AR 124881A2
Authority
AR
Argentina
Prior art keywords
stem tissue
lower stem
alfalfa
alfalfa plant
dna molecule
Prior art date
Application number
ARP220100303A
Other languages
English (en)
Inventor
William Hiatt
Marry S Antes Conocido Como Reddy Srinu Reddy
Mark Mcaslin
Stephen Temple
David Whalen
Richard Eric Cerny
Original Assignee
Monsanto Technology Llc
Forage Genetics International Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc, Forage Genetics International Llc filed Critical Monsanto Technology Llc
Publication of AR124881A2 publication Critical patent/AR124881A2/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8255—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving lignin biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/0098—Plants or trees
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Pathology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Fodder In General (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La invención provee un método para la selección de una planta de alfalfa con mejorado valor de alimentación y mayor flexibilidad en la gestión, ya sea para incrementar la calidad de forraje proporcionada, ya sea para mejorar la biomasa disponible con anterioridad. Reivindicación 1: Un método para la selección de una planta de alfalfa con componentes de valor nutricional mejorado, a partir de una población de plantas de alfalfa, donde dicha población de plantas de alfalfa comprende una molécula de ADN recombinado, y donde una porción de dicha molécula de ADN recombinado es homóloga o complementaria a SEC. ID. Nº 1 o SEC. ID. Nº 2, que comprende: (a) la medición de la concentración de lignina detergente ácida (ADL) en un tejido de tallo inferior de cada una de dichas plantas de alfalfa en dicha población de plantas de alfalfa, donde dicha concentración de ADL se reduce entre 12% y aproximadamente 31% en comparación con un tejido de tallo inferior de una planta de alfalfa que no comprende dicha molécula de ADN; (b) la medición de la concentración de guaiacil lignina (G lignina) en dicho tejido de tallo inferior, donde dicho contenido de G lignina es reducido alrededor de 25% o más en dicho tejido de tallo inferior, en comparación con un tejido de tallo inferior de una planta de alfalfa que no comprende dicha molécula de ADN; (c) la medición del componente de digestibilidad de fibra detergente neutra (NDFD) en dicho tejido de tallo inferior, donde dicho valor de NDFD está aumentado más de 18% en dicho tejido de tallo inferior en comparación con un tejido de tallo inferior de una planta de alfalfa que no comprende dicha molécula de ADN; (d) la selección de una planta de alfalfa que comprende los componentes de valor nutricional mejorado de las etapas (a) - (c); y (e) el cultivo de dicha planta de alfalfa seleccionada en la etapa (d).
ARP220100303A 2012-06-26 2022-02-15 Métodos y composiciones para calidad de forraje mejorada AR124881A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201261664359P 2012-06-26 2012-06-26

Publications (1)

Publication Number Publication Date
AR124881A2 true AR124881A2 (es) 2023-05-17

Family

ID=49784009

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP130102260 AR091572A1 (es) 2012-06-26 2013-06-26 Metodos y composiciones para calidad de forraje mejorada
ARP220100303A AR124881A2 (es) 2012-06-26 2022-02-15 Métodos y composiciones para calidad de forraje mejorada

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ARP130102260 AR091572A1 (es) 2012-06-26 2013-06-26 Metodos y composiciones para calidad de forraje mejorada

Country Status (13)

Country Link
US (2) US9771597B2 (es)
EP (1) EP2864486B1 (es)
JP (2) JP2015527878A (es)
CN (1) CN104603275A (es)
AR (2) AR091572A1 (es)
AU (1) AU2013280398B2 (es)
BR (2) BR112014032343A2 (es)
CA (1) CA2877873C (es)
MX (1) MX362498B (es)
NZ (1) NZ629197A (es)
PL (1) PL2864486T3 (es)
WO (1) WO2014004683A2 (es)
ZA (1) ZA201408419B (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9854778B2 (en) 2011-06-30 2018-01-02 Monsanto Technology Llc Alfalfa plant and seed corresponding to transgenic event KK 179-2 and methods for detection thereof
WO2013003558A1 (en) 2011-06-30 2013-01-03 Monsanto Technology Llc Alfalfa plant and seed corresponding to transgenic event kk 179-2 and methods for detection thereof
PL2864486T3 (pl) * 2012-06-26 2019-05-31 Forage Genetics Int Llc Sposoby i kompozycje do zwiększania jakości paszy
WO2016054071A1 (en) * 2014-09-29 2016-04-07 Alforex Seeds LLC Low lignin non-transgenic alfalfa varieties and methods for producing the same
KR102198787B1 (ko) * 2019-09-05 2021-01-05 한국과학기술연구원 변이된 comt 유전자를 포함하는 사료용 작물 및 이의 용도

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Also Published As

Publication number Publication date
BR122019026534B1 (pt) 2022-07-19
AU2013280398A1 (en) 2014-12-04
WO2014004683A3 (en) 2014-04-24
EP2864486A4 (en) 2016-03-02
ZA201408419B (en) 2015-12-23
EP2864486B1 (en) 2018-09-26
BR112014032343A2 (pt) 2017-08-01
AU2013280398B2 (en) 2018-07-12
US9771597B2 (en) 2017-09-26
CA2877873C (en) 2020-11-24
CA2877873A1 (en) 2014-01-03
PL2864486T3 (pl) 2019-05-31
CN104603275A (zh) 2015-05-06
EP2864486A2 (en) 2015-04-29
WO2014004683A2 (en) 2014-01-03
JP2015527878A (ja) 2015-09-24
MX362498B (es) 2019-01-21
NZ629197A (en) 2016-07-29
US20180080038A1 (en) 2018-03-22
AR091572A1 (es) 2015-02-11
JP2018108079A (ja) 2018-07-12
MX2014015422A (es) 2015-04-09
US20150135370A1 (en) 2015-05-14
US10704053B2 (en) 2020-07-07

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