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AR124505A2 - COMPOSITIONS RELATED TO A MUTANT CLOSTRIDIUM DIFFICILE TOXIN AND THEIR METHODS - Google Patents

COMPOSITIONS RELATED TO A MUTANT CLOSTRIDIUM DIFFICILE TOXIN AND THEIR METHODS

Info

Publication number
AR124505A2
AR124505A2 ARP210103660A ARP210103660A AR124505A2 AR 124505 A2 AR124505 A2 AR 124505A2 AR P210103660 A ARP210103660 A AR P210103660A AR P210103660 A ARP210103660 A AR P210103660A AR 124505 A2 AR124505 A2 AR 124505A2
Authority
AR
Argentina
Prior art keywords
difficile toxin
mutant
clostridium difficile
methods
relates
Prior art date
Application number
ARP210103660A
Other languages
Spanish (es)
Inventor
Annaliesa Sybil Anderson
Robert G K Donald
Michael James Flint
Kathrin Ute Jansen
Narender K Kalyan
Terri L Mininni
Justin Keith Moran
Mark E Ruppen
Maninder K Sidhu
Original Assignee
Wyeth Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wyeth Llc filed Critical Wyeth Llc
Publication of AR124505A2 publication Critical patent/AR124505A2/en

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  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)

Abstract

En un aspecto, la invención se refiere a una composición inmunogénica que incluye una toxina A de Clostridium difficile mutante y/o una toxina B de Clostridium difficile mutante. Cada toxina mutante incluye un dominio de glucosiltransferasa que tiene al menos una mutación y un dominio de cisteína proteasa que tiene al menos una mutación, con respecto a la correspondiente toxina de C. difficile tipo salvaje. Las toxinas mutantes también pueden incluir al menos un aminoácido que está entrecruzado químicamente. En otro aspecto, la invención se refiere a anticuerpos o fragmentos de unión a este que se unen a dichas composiciones inmunogénicas. En un aspecto adicional, la invención se refiere a secuencias de nucleótidos aisladas que codifican alguna de los precedentes y los métodos de cualquiera de las composiciones precedentes. Reivindicación 1: Un polipéptido aislado, caracterizado porque comprende la secuencia de aminoácidos de SEQ ID Nº 6, donde el residuo metionina en la posición 1 de SEQ ID Nº 6 no está presente, donde un residuo lisina del polipéptido está entrecruzado a un grupo b-alanina.In one aspect, the invention relates to an immunogenic composition including a mutant Clostridium difficile toxin A and/or a mutant Clostridium difficile toxin B. Each mutant toxin includes a glycosyltransferase domain having at least one mutation and a cysteine protease domain having at least one mutation, relative to the corresponding wild-type C. difficile toxin. The mutant toxins can also include at least one amino acid that is chemically cross-linked. In another aspect, the invention relates to antibodies or binding fragments thereto that bind to said immunogenic compositions. In a further aspect, the invention relates to isolated nucleotide sequences encoding any of the foregoing and methods of any of the foregoing compositions. Claim 1: An isolated polypeptide, characterized in that it comprises the amino acid sequence of SEQ ID No. 6, where the methionine residue at position 1 of SEQ ID No. 6 is not present, where a lysine residue of the polypeptide is crosslinked to a b-group to the girl.

ARP210103660A 2011-04-22 2021-12-23 COMPOSITIONS RELATED TO A MUTANT CLOSTRIDIUM DIFFICILE TOXIN AND THEIR METHODS AR124505A2 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201161478474P 2011-04-22 2011-04-22

Publications (1)

Publication Number Publication Date
AR124505A2 true AR124505A2 (en) 2023-04-05

Family

ID=86380134

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP210103660A AR124505A2 (en) 2011-04-22 2021-12-23 COMPOSITIONS RELATED TO A MUTANT CLOSTRIDIUM DIFFICILE TOXIN AND THEIR METHODS

Country Status (1)

Country Link
AR (1) AR124505A2 (en)

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