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AR110908A1 - Constructo promotor para la síntesis de proteínas libre de células - Google Patents

Constructo promotor para la síntesis de proteínas libre de células

Info

Publication number
AR110908A1
AR110908A1 ARP180100165A ARP180100165A AR110908A1 AR 110908 A1 AR110908 A1 AR 110908A1 AR P180100165 A ARP180100165 A AR P180100165A AR P180100165 A ARP180100165 A AR P180100165A AR 110908 A1 AR110908 A1 AR 110908A1
Authority
AR
Argentina
Prior art keywords
synthesis
cells
proteins free
promoter construction
free
Prior art date
Application number
ARP180100165A
Other languages
English (en)
Inventor
Dr Schillberg Stefan Prof
Simon Vogel
Matthias Buntru
Original Assignee
Fraunhofer Ges Forschung
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fraunhofer Ges Forschung filed Critical Fraunhofer Ges Forschung
Publication of AR110908A1 publication Critical patent/AR110908A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Constructos promotores, métodos y sistemas para un aumento en el rendimiento de la proteína diana y/o permitir el uso de menor concentración de plantilla en los sistemas de síntesis de proteínas libre de células.
ARP180100165A 2017-01-25 2018-01-24 Constructo promotor para la síntesis de proteínas libre de células AR110908A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP17153067.8A EP3354736B1 (en) 2017-01-25 2017-01-25 Promoter construct for cell-free protein synthesis

Publications (1)

Publication Number Publication Date
AR110908A1 true AR110908A1 (es) 2019-05-15

Family

ID=58016526

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP180100165A AR110908A1 (es) 2017-01-25 2018-01-24 Constructo promotor para la síntesis de proteínas libre de células

Country Status (7)

Country Link
US (1) US11371047B2 (es)
EP (2) EP3354736B1 (es)
JP (1) JP7048643B2 (es)
CN (1) CN110199025B (es)
AR (1) AR110908A1 (es)
CA (1) CA3049826C (es)
WO (1) WO2018138201A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3354736B1 (en) 2017-01-25 2020-07-01 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Promoter construct for cell-free protein synthesis
WO2021058145A1 (en) * 2019-09-24 2021-04-01 Max-Delbrück-Centrum Für Molekulare Medizin In Der Helmholtz-Gemeinschaft Phage t7 promoters for boosting in vitro transcription
CN111019963B (zh) * 2019-12-30 2021-07-16 苏州珀罗汀生物技术有限公司 无细胞表达rhUTI蛋白系统及生产rhUTI蛋白的方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0494955B1 (en) * 1989-10-05 1998-07-15 Optein, Inc. Cell-free synthesis and isolation of novel genes and polypeptides
JPH1156363A (ja) * 1997-08-19 1999-03-02 Nippon Flour Mills Co Ltd タンパクの製造方法
US6168931B1 (en) 1999-03-17 2001-01-02 The Board Of Trustees Of The Leland Stanford Junior University Enhanced in vitro synthesis of biological macromolecules using a novel ATP regeneration system
US6337191B1 (en) 1999-03-22 2002-01-08 The Board Of Trustees Of The Leland Stanford Junior University Vitro protein synthesis using glycolytic intermediates as an energy source
JP4079640B2 (ja) * 2000-04-27 2008-04-23 バイオリーダーズ コーポレーション 新規なプロモーターおよび該プロモーターを用いた遺伝子発現方法
JP4762481B2 (ja) 2000-08-30 2011-08-31 株式会社セルフリーサイエンス 無細胞タンパク質合成用転写鋳型の設計および構築、並びにこれを用いる希釈バッチ方式コムギ胚芽無細胞タンパク質合成法
JP2004290181A (ja) * 2003-03-13 2004-10-21 National Institute Of Advanced Industrial & Technology 低温におけるポリペプチドの無細胞翻訳
CN101631868B (zh) * 2007-02-16 2016-02-10 巴斯福植物科学有限公司 用于在单子叶植物中调节胚特异性表达的核酸序列
EP3354736B1 (en) 2017-01-25 2020-07-01 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Promoter construct for cell-free protein synthesis

Also Published As

Publication number Publication date
JP2020505951A (ja) 2020-02-27
US20190376068A1 (en) 2019-12-12
EP3574099A1 (en) 2019-12-04
EP3574099C0 (en) 2023-10-25
EP3354736B1 (en) 2020-07-01
CN110199025B (zh) 2022-11-18
EP3354736A1 (en) 2018-08-01
JP7048643B2 (ja) 2022-04-05
CA3049826A1 (en) 2018-08-02
CA3049826C (en) 2023-01-24
EP3574099B1 (en) 2023-10-25
WO2018138201A1 (en) 2018-08-02
CN110199025A (zh) 2019-09-03
US11371047B2 (en) 2022-06-28

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