AR118854A1 - METHODS TO REDUCE THE RATE OF ENZYMATIC HYDROLYSIS ACTIVITY IN A COMPOSITION OBTAINED FROM A PURIFICATION PLATFORM - Google Patents
METHODS TO REDUCE THE RATE OF ENZYMATIC HYDROLYSIS ACTIVITY IN A COMPOSITION OBTAINED FROM A PURIFICATION PLATFORMInfo
- Publication number
- AR118854A1 AR118854A1 ARP200101263A ARP200101263A AR118854A1 AR 118854 A1 AR118854 A1 AR 118854A1 AR P200101263 A ARP200101263 A AR P200101263A AR P200101263 A ARP200101263 A AR P200101263A AR 118854 A1 AR118854 A1 AR 118854A1
- Authority
- AR
- Argentina
- Prior art keywords
- purification
- enzymatic hydrolysis
- rate
- hydrolysis activity
- methods
- Prior art date
Links
- 238000000746 purification Methods 0.000 title abstract 9
- 238000000034 method Methods 0.000 title abstract 5
- 239000000203 mixture Substances 0.000 title abstract 4
- 230000000694 effects Effects 0.000 title abstract 3
- 230000007071 enzymatic hydrolysis Effects 0.000 title abstract 3
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 title abstract 3
- 238000011118 depth filtration Methods 0.000 abstract 2
- 238000004191 hydrophobic interaction chromatography Methods 0.000 abstract 2
- 239000008194 pharmaceutical composition Substances 0.000 abstract 1
- 229920001184 polypeptide Polymers 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
La presente descripción proporciona plataformas de purificación que comprenden una etapa de filtro de profundidad y/o una etapa de cromatografía de interacción hidrófoba (HIC). También se describen en el presente documento métodos para usar las plataformas de purificación descritas en el presente y composiciones obtenidas a partir de los mismos, tales como composiciones farmacéuticas. Reivindicación 1: Un método para reducir una tasa de actividad de hidrólisis enzimática de una composición obtenida a partir de una plataforma de purificación, el método comprende someter una muestra a la plataforma de purificación, caracterizado porque comprende: (a) una etapa de captura; y (b) una etapa de filtración de profundidad, reduciendo de ese modo la tasa de actividad de hidrólisis enzimática de la composición, en comparación con la purificación de la muestra mediante el uso de la misma plataforma de purificación sin la etapa de filtración de profundidad. Reivindicación 24: El método de la reivindicación 23, caracterizado porque las una o más etapas de purificación comprenden etapas de purificación de polipéptidos.The present disclosure provides purification platforms comprising a depth filter stage and/or a hydrophobic interaction chromatography (HIC) stage. Also described herein are methods of using the purification platforms described herein and compositions made therefrom, such as pharmaceutical compositions. Claim 1: A method for reducing an enzymatic hydrolysis activity rate of a composition obtained from a purification platform, the method comprises subjecting a sample to the purification platform, characterized in that it comprises: (a) a capture step; and (b) a depth filtration step, thereby reducing the rate of enzymatic hydrolysis activity of the composition, compared to sample purification using the same purification platform without the depth filtration step. . Claim 24: The method of claim 23, characterized in that the one or more purification steps comprise polypeptide purification steps.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962843261P | 2019-05-03 | 2019-05-03 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR118854A1 true AR118854A1 (en) | 2021-11-03 |
Family
ID=80053340
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP200101263A AR118854A1 (en) | 2019-05-03 | 2020-05-04 | METHODS TO REDUCE THE RATE OF ENZYMATIC HYDROLYSIS ACTIVITY IN A COMPOSITION OBTAINED FROM A PURIFICATION PLATFORM |
Country Status (1)
| Country | Link |
|---|---|
| AR (1) | AR118854A1 (en) |
-
2020
- 2020-05-04 AR ARP200101263A patent/AR118854A1/en unknown
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