AR116403A1 - Nucleasa pacas9 - Google Patents
Nucleasa pacas9Info
- Publication number
- AR116403A1 AR116403A1 ARP190102604A ARP190102604A AR116403A1 AR 116403 A1 AR116403 A1 AR 116403A1 AR P190102604 A ARP190102604 A AR P190102604A AR P190102604 A ARP190102604 A AR P190102604A AR 116403 A1 AR116403 A1 AR 116403A1
- Authority
- AR
- Argentina
- Prior art keywords
- nuclease
- pacas9
- nucleic acid
- refers
- seq
- Prior art date
Links
- 101710163270 Nuclease Proteins 0.000 abstract 12
- 150000007523 nucleic acids Chemical class 0.000 abstract 6
- 102000039446 nucleic acids Human genes 0.000 abstract 6
- 108020004707 nucleic acids Proteins 0.000 abstract 6
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 239000013604 expression vector Substances 0.000 abstract 2
- 238000004519 manufacturing process Methods 0.000 abstract 2
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 239000002502 liposome Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/127—Synthetic bilayered vehicles, e.g. liposomes or liposomes with cholesterol as the only non-phosphatidyl surfactant
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/007—Vectors comprising a special translation-regulating system cell or tissue specific
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Mycology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Dispersion Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicinal Preparation (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
La presente se refiere al campo de la biotecnología, la biología molecular y la medicina; en particular, a la enzima nucleasa y su uso. Más específicamente, la presente se refiere a la enzima nucleasa PaCas9. La presente también se refiere a un ácido nucleico que codifica dicha nucleasa, un constructo genético, un vector de expresión, un vector de administración que comprende dicho ácido nucleico, un liposoma que comprende dicha nucleasa o ácido nucleico que codifica dicha nucleasa, un método para producir una nucleasa, métodos para la administración, y una célula hospedadora que comprende un ácido nucleico que codifica dicha nucleasa. Reivindicación 1: Nucleasa PaCas9 que tiene una secuencia de aminoácidos de la SEQ ID Nº 2. Reivindicación 2: Una molécula de ácido nucleico aislada que codifica una nucleasa PaCas9 según la reivindicación 1, y que tiene una secuencia de nucleótidos de la SEQ ID Nº 1. Reivindicación 3: Un vector de expresión que comprende un ácido nucleico según la reivindicación 2. Reivindicación 12: Un método para producir una nucleasa PaCas9 según la reivindicación 1, que comprende cultivar una célula hospedadora según la reivindicación 10 en un medio de crecimiento en condiciones suficientes para producir dicha nucleasa PaCas9, si es necesario, seguido por el aislamiento y la purificación de la nucleasa PaCas9 obtenida.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| RU2018132816A RU2706298C1 (ru) | 2018-09-14 | 2018-09-14 | НУКЛЕАЗА PaCas9 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR116403A1 true AR116403A1 (es) | 2021-05-05 |
Family
ID=68580023
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP190102604A AR116403A1 (es) | 2018-09-14 | 2019-09-13 | Nucleasa pacas9 |
Country Status (19)
| Country | Link |
|---|---|
| US (1) | US12173336B2 (es) |
| EP (1) | EP3851522A4 (es) |
| JP (1) | JP7693536B2 (es) |
| CN (1) | CN113272425B (es) |
| AR (1) | AR116403A1 (es) |
| AU (1) | AU2019341014B2 (es) |
| BR (1) | BR112021004746A2 (es) |
| CA (1) | CA3113215A1 (es) |
| CL (1) | CL2021000610A1 (es) |
| CO (1) | CO2021003285A2 (es) |
| EA (1) | EA202190676A1 (es) |
| MA (1) | MA53045B1 (es) |
| MX (1) | MX2021002933A (es) |
| PE (1) | PE20211111A1 (es) |
| PH (1) | PH12021550563A1 (es) |
| RU (1) | RU2706298C1 (es) |
| TW (1) | TWI843749B (es) |
| WO (1) | WO2020055293A1 (es) |
| ZA (1) | ZA202101681B (es) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2722934C1 (ru) * | 2019-06-11 | 2020-06-05 | Автономная некоммерческая образовательная организация высшего образования Сколковский институт науки и технологий | Средство разрезания днк на основе cas9 белка из бактерии pasteurella pneumotropica |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4399216A (en) | 1980-02-25 | 1983-08-16 | The Trustees Of Columbia University | Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials |
| US4634665A (en) | 1980-02-25 | 1987-01-06 | The Trustees Of Columbia University In The City Of New York | Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials |
| US5179017A (en) | 1980-02-25 | 1993-01-12 | The Trustees Of Columbia University In The City Of New York | Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials |
| US4510245A (en) | 1982-11-18 | 1985-04-09 | Chiron Corporation | Adenovirus promoter system |
| US4740461A (en) | 1983-12-27 | 1988-04-26 | Genetics Institute, Inc. | Vectors and methods for transformation of eucaryotic cells |
| US5168062A (en) | 1985-01-30 | 1992-12-01 | University Of Iowa Research Foundation | Transfer vectors and microorganisms containing human cytomegalovirus immediate-early promoter-regulatory DNA sequence |
| WO1986005807A1 (en) | 1985-04-01 | 1986-10-09 | Celltech Limited | Transformed myeloma cell-line and a process for the expression of a gene coding for a eukaryotic polypeptide employing same |
| US4968615A (en) | 1985-12-18 | 1990-11-06 | Ciba-Geigy Corporation | Deoxyribonucleic acid segment from a virus |
| GB8601597D0 (en) | 1986-01-23 | 1986-02-26 | Wilson R H | Nucleotide sequences |
| US4959455A (en) | 1986-07-14 | 1990-09-25 | Genetics Institute, Inc. | Primate hematopoietic growth factors IL-3 and pharmaceutical compositions |
| US4912040A (en) | 1986-11-14 | 1990-03-27 | Genetics Institute, Inc. | Eucaryotic expression system |
| GB8717430D0 (en) | 1987-07-23 | 1987-08-26 | Celltech Ltd | Recombinant dna product |
| GB8809129D0 (en) | 1988-04-18 | 1988-05-18 | Celltech Ltd | Recombinant dna methods vectors and host cells |
| US9410134B2 (en) * | 2011-06-07 | 2016-08-09 | Helmholtz Zentrum München—Deutsches Forschungszentrum für Gesundheit und Umwelt | Protein having nuclease activity, fusion proteins and uses thereof |
| CN104471067B (zh) | 2012-05-07 | 2020-08-14 | 桑格摩生物治疗股份有限公司 | 用于核酸酶介导的转基因靶向整合的方法和组合物 |
| PE20190842A1 (es) * | 2012-05-25 | 2019-06-17 | Emmanuelle Charpentier | Arn de direccion a adn de dos moleculas |
| JP6368304B2 (ja) * | 2012-06-29 | 2018-08-01 | コンセホ スペリオール デ インベスティガシオネス シエンティフィカス（セエセイセ）Ｃｏｎｓｅｊｏ Ｓｕｐｅｒｉｏｒ Ｄｅ Ｉｎｖｅｓｔｉｇａｃｉｏｎｅｓ Ｃｉｅｎｔｉｆｉｃａｓ（Ｃｓｉｃ） | 生物活性化合物の送達に有用な機能性リポソーム |
| WO2017048969A1 (en) * | 2015-09-17 | 2017-03-23 | The Regents Of The University Of California | Variant cas9 polypeptides comprising internal insertions |
| RU2634395C1 (ru) * | 2015-12-01 | 2017-10-26 | Федеральное государственное автономное образовательное учреждение высшего профессионального образования "Балтийский Федеральный Университет имени Иммануила Канта" (БФУ им. И. Канта) | Генетическая конструкция на основе системы редактирования генома crispr/cas9, кодирующая нуклеазу cas9, специфически импортируемую в митохондрии клеток человека |
| JP7305534B2 (ja) * | 2016-07-06 | 2023-07-10 | バーテックス ファーマシューティカルズ インコーポレイテッド | 疼痛関連障害を処置するための物質及び方法 |
| WO2018172556A1 (en) * | 2017-03-24 | 2018-09-27 | Curevac Ag | Nucleic acids encoding crispr-associated proteins and uses thereof |
-
2018
- 2018-09-14 RU RU2018132816A patent/RU2706298C1/ru active
-
2019
- 2019-09-12 TW TW108133034A patent/TWI843749B/zh active
- 2019-09-13 AU AU2019341014A patent/AU2019341014B2/en active Active
- 2019-09-13 CA CA3113215A patent/CA3113215A1/en active Pending
- 2019-09-13 BR BR112021004746-8A patent/BR112021004746A2/pt unknown
- 2019-09-13 PE PE2021000323A patent/PE20211111A1/es unknown
- 2019-09-13 EP EP19858873.3A patent/EP3851522A4/en active Pending
- 2019-09-13 MX MX2021002933A patent/MX2021002933A/es unknown
- 2019-09-13 US US17/276,016 patent/US12173336B2/en active Active
- 2019-09-13 AR ARP190102604A patent/AR116403A1/es unknown
- 2019-09-13 EA EA202190676A patent/EA202190676A1/ru unknown
- 2019-09-13 WO PCT/RU2019/050154 patent/WO2020055293A1/ru not_active Ceased
- 2019-09-13 CN CN201980075402.9A patent/CN113272425B/zh active Active
- 2019-09-13 MA MA53045A patent/MA53045B1/fr unknown
- 2019-09-13 JP JP2021513998A patent/JP7693536B2/ja active Active
-
2021
- 2021-03-12 CO CONC2021/0003285A patent/CO2021003285A2/es unknown
- 2021-03-12 ZA ZA2021/01681A patent/ZA202101681B/en unknown
- 2021-03-12 CL CL2021000610A patent/CL2021000610A1/es unknown
- 2021-03-15 PH PH12021550563A patent/PH12021550563A1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PE20211111A1 (es) | 2021-06-21 |
| CL2021000610A1 (es) | 2021-09-24 |
| AU2019341014A1 (en) | 2021-05-13 |
| MX2021002933A (es) | 2021-06-15 |
| CN113272425B (zh) | 2024-09-13 |
| US12173336B2 (en) | 2024-12-24 |
| EA202190676A1 (ru) | 2021-06-08 |
| CO2021003285A2 (es) | 2021-06-10 |
| US20220064612A1 (en) | 2022-03-03 |
| JP2022500044A (ja) | 2022-01-04 |
| ZA202101681B (en) | 2022-06-29 |
| TW202016130A (zh) | 2020-05-01 |
| PH12021550563A1 (en) | 2022-02-14 |
| WO2020055293A1 (ru) | 2020-03-19 |
| EP3851522A1 (en) | 2021-07-21 |
| AU2019341014B2 (en) | 2025-04-24 |
| EP3851522A4 (en) | 2022-06-01 |
| BR112021004746A2 (pt) | 2021-06-08 |
| TWI843749B (zh) | 2024-06-01 |
| RU2706298C1 (ru) | 2019-11-15 |
| KR20210062040A (ko) | 2021-05-28 |
| MA53045A1 (fr) | 2022-02-28 |
| CN113272425A (zh) | 2021-08-17 |
| JP7693536B2 (ja) | 2025-06-17 |
| MA53045B1 (fr) | 2022-08-31 |
| CA3113215A1 (en) | 2020-03-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106544351B (zh) | CRISPR-Cas9体外敲除耐药基因mcr-1的方法及其专用细胞穿透肽 | |
| JP6284181B2 (ja) | 環状ｒｎａ及びタンパク質の製造方法 | |
| ES2750525T3 (es) | Procedimientos y composiciones relacionados con CRM197 | |
| ES2575252T3 (es) | Proceso para la producción de ácido hialurónico en Escherichia coli o Bacillus megaterium | |
| NZ609903A (en) | Expression vector for high level expression of recombinant proteins | |
| EP4527459A3 (en) | Nucleic acid, composition and conjugate containing nucleic acid, preparation method therefor and use thereof | |
| WO2020198509A3 (en) | Modified oligonucleotides with increased stability | |
| HRP20180282T1 (hr) | Imunoterapija na bazi indoleamin 2, 3-dioksigenaze | |
| BR112015023424A2 (pt) | construto de expressão engenheirado, método para aprimoramento de produção de rna ou proteína, vetor, célula, hospedeira bacteriana, sistema de cultura de célula para síntese in vivo de dsrna, composição e método para controlar uma infestação de pragas de invertebrados ou para inibir a propagação de uma doença viral em uma população de plantas, e terminador transcricional | |
| AR090417A1 (es) | Plantas resistentes al glifosato y metodos asociados | |
| RU2014128987A (ru) | Быстрый способ клонирования и экспрессии сегментов гена родственной вариабельной области антитела | |
| US20100008939A1 (en) | Unprocessed rolling circle amplification product | |
| PH12012502520A1 (en) | Plants having enhanced yield-related traits and method for making the same | |
| UA115235C2 (uk) | Ген токсину axmi335 bacillus thuringiensis та спосіб його застосування | |
| RU2006145713A (ru) | Способ продукции пуриновых нуклиозидов и нуклиотидов методом ферментации с использованием бактерий, принадлежащих к роду escherichia или bacillus | |
| EA201070229A1 (ru) | Нуклеотидные последовательности msca1, влияющие на мужскую фертильность растений, и способ их применения | |
| BRPI0507077A (pt) | processo para a preparação de l-aminoácidos usando cepas da famìlia enterobacteriaceae | |
| CN119452085A (zh) | 具有ruvc结构域的酶 | |
| AR116403A1 (es) | Nucleasa pacas9 | |
| RU2016152662A (ru) | Микроорганизм рода Escherichia, продуцирующий L-триптофан, и способ продуцирования L-триптофана с использованием данного микроорганизма | |
| AR102803A1 (es) | Vacunas de reovirus aviar | |
| Yu et al. | AIV polyantigen epitope expressed by recombinant baculovirus induces a systemic immune response in chicken and mouse models | |
| AR102762A1 (es) | Secuencia de oligonucleótidos para uso en ingeniería de rutas metabólicas | |
| SG10201809464RA (en) | Antibody gene expression-secretion system | |
| CN118139979A (zh) | 具有hepn结构域的酶 |