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AR115417A1 - Método para clasificar material vegetal - Google Patents

Método para clasificar material vegetal

Info

Publication number
AR115417A1
AR115417A1 ARP190101387A ARP190101387A AR115417A1 AR 115417 A1 AR115417 A1 AR 115417A1 AR P190101387 A ARP190101387 A AR P190101387A AR P190101387 A ARP190101387 A AR P190101387A AR 115417 A1 AR115417 A1 AR 115417A1
Authority
AR
Argentina
Prior art keywords
plant material
nicotiana
sterility
batch
sequence
Prior art date
Application number
ARP190101387A
Other languages
English (en)
Inventor
Nicolas Jean Bakaher
Original Assignee
Philip Morris Products Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Philip Morris Products Sa filed Critical Philip Morris Products Sa
Publication of AR115417A1 publication Critical patent/AR115417A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se describe en la presente descripción un método para clasificar un lote de material vegetal como que está contaminado o no contaminado que comprende: (i) proporcionar una muestre de polinucleótido del lote de material vegetal; (ii) poner en contacto la muestra de polinucleótido con uno o más cebadores de amplificación para amplificar una secuencia de polinucleótidos objetivo asociada con la esterilidad; (iii) realizar una reacción in vitro de amplificación de polinucleótidos en la muestra para generar uno o más productos de amplificación, (iv) determinar el tamaño o la secuencia del(de los) producto(s) de amplificación, y (v) basado en el tamaño o la secuencia del(de los) producto(s) de amplificación determinados en la etapa (iv) comparar el tamaño o la secuencia del(de los) producto(s) de amplificación con el tamaño o la secuencia del(de los) producto(s) de amplificación conocidos de fuentes genéticas de esterilidad conocidas; en donde si la fuente genética de esterilidad determinada en la etapa (v) corresponde a la fuente genética de esterilidad esperada del lote de material vegetal, entonces dicho lote de material vegetal se considera no contaminado; o en donde si la fuente genética de esterilidad determinada en la etapa (v) no corresponde a la fuente genética de esterilidad esperada del lote de material vegetal, entonces dicho lote de material vegetal se considera contaminado. Reivindicación 8: El método de conformidad con cualquiera de las reivindicaciones anteriores, en donde el lote de material vegetal que va a clasificarse es material vegetal de Nicotiana tabacum y en donde la fuente genética de esterilidad esperada es la esterilidad masculina citoplasmática de Nicotiana suaveolens, Nicotiana glauca, Nicotiana bigelovii, Nicotiana plumbaginifolia, Nicotiana megalosiphon o Nicotiana undulata, adecuadamente, Nicotiana suaveolens o Nicotiana glauca.
ARP190101387A 2018-05-25 2019-05-23 Método para clasificar material vegetal AR115417A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP18305638 2018-05-25

Publications (1)

Publication Number Publication Date
AR115417A1 true AR115417A1 (es) 2021-01-13

Family

ID=62528373

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP190101387A AR115417A1 (es) 2018-05-25 2019-05-23 Método para clasificar material vegetal

Country Status (7)

Country Link
US (1) US20210222262A1 (es)
EP (1) EP3802886A1 (es)
KR (1) KR20210013180A (es)
CN (1) CN112189056B (es)
AR (1) AR115417A1 (es)
BR (1) BR112020022675A2 (es)
WO (1) WO2019224298A1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110592265B (zh) * 2019-10-29 2023-11-24 江西省农业科学院蔬菜花卉研究所 一种用于茄属植物快速鉴定的dna条形码及方法
US11434525B2 (en) 2020-08-06 2022-09-06 Singular Genomics Systems, Inc. Spatial sequencing
WO2022032194A1 (en) 2020-08-06 2022-02-10 Singular Genomics Systems, Inc. Methods for in situ transcriptomics and proteomics
CN116656857A (zh) * 2020-12-04 2023-08-29 南京林业大学 一组用于华东木犀物种的特异性叶绿体基因变异位点鉴定的dna探针及其鉴定方法
EP4334706A4 (en) 2021-05-05 2025-08-06 Singular Genomics Systems Inc MULTIOMICS ANALYSIS DEVICE AND METHODS OF USE THEREOF
CN114032329A (zh) * 2021-12-02 2022-02-11 云南省烟草农业科学研究院 叶绿体分子标记引物及用于早期鉴定烟草品种种子来源的应用
WO2024249961A1 (en) 2023-06-01 2024-12-05 Singular Genomics Systems, Inc. Methods and probes for detecting polynucleotide sequences in cells and tissues

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ221375A (en) * 1987-07-30 1990-09-26 Nz Scientific & Ind Res Hybrid seed production using a phytotoxic chemical to eliminate undesirable seeds
FR2667078B1 (fr) * 1990-09-21 1994-09-16 Agronomique Inst Nat Rech Sequence d'adn conferant une sterilite male cytoplasmique, genome mitochondrial, mitochondrie et plante contenant cette sequence, et procede de preparation d'hybrides.
US6045996A (en) 1993-10-26 2000-04-04 Affymetrix, Inc. Hybridization assays on oligonucleotide arrays
US6068818A (en) 1993-11-01 2000-05-30 Nanogen, Inc. Multicomponent devices for molecular biological analysis and diagnostics
US5888750A (en) 1997-05-30 1999-03-30 Synsorb Biotech, Inc. Method of recovering shiga-like toxins and vaccines comprising inactivated shiga-like toxin
US6429027B1 (en) 1998-12-28 2002-08-06 Illumina, Inc. Composite arrays utilizing microspheres
WO2002077277A2 (en) * 2001-03-26 2002-10-03 Council Of Scientific And Industrial Research Dna markers for assessing seed purity and a method of using dna sequences for assessing seed purity
FR2876378B1 (fr) * 2004-10-08 2007-01-05 Univ Joseph Fourier Etablissem Amorces universelles et leur utilisation pour la detection et l'identification de vegetaux dans des melanges complexes
WO2013080045A2 (en) * 2011-11-28 2013-06-06 Anglo Netherlands Grain B.V. Method for differentiating fertile and sterile plant lines by detection of polymorphic markers in chloroplast dna

Also Published As

Publication number Publication date
BR112020022675A2 (pt) 2021-02-17
CN112189056A (zh) 2021-01-05
KR20210013180A (ko) 2021-02-03
WO2019224298A1 (en) 2019-11-28
US20210222262A1 (en) 2021-07-22
CN112189056B (zh) 2025-01-17
EP3802886A1 (en) 2021-04-14

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