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AR114548A1 - Estrategias de cultivo celular para modular la glicosilación de proteínas - Google Patents

Estrategias de cultivo celular para modular la glicosilación de proteínas

Info

Publication number
AR114548A1
AR114548A1 ARP190102268A ARP190102268A AR114548A1 AR 114548 A1 AR114548 A1 AR 114548A1 AR P190102268 A ARP190102268 A AR P190102268A AR P190102268 A ARP190102268 A AR P190102268A AR 114548 A1 AR114548 A1 AR 114548A1
Authority
AR
Argentina
Prior art keywords
cell culture
modulate
glycosylation
proteins
strategies
Prior art date
Application number
ARP190102268A
Other languages
English (en)
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of AR114548A1 publication Critical patent/AR114548A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La materia descrita en la presente hace referencia a medios de cultivo celular y estrategias de cultivo celular para modular el patrón de glicosilación, por ejemplo, el patrón de fucosilación y/o galactosilación, de una glicoproteína de interés, por ejemplo, un anticuerpo, así como también composiciones de glicoproteínas y cultivos celulares preparados usando dichos medios y/o estrategias.
ARP190102268A 2018-08-10 2019-08-09 Estrategias de cultivo celular para modular la glicosilación de proteínas AR114548A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201862717751P 2018-08-10 2018-08-10

Publications (1)

Publication Number Publication Date
AR114548A1 true AR114548A1 (es) 2020-09-16

Family

ID=67902575

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP190102268A AR114548A1 (es) 2018-08-10 2019-08-09 Estrategias de cultivo celular para modular la glicosilación de proteínas

Country Status (14)

Country Link
US (1) US20210238644A1 (es)
EP (1) EP3833688A1 (es)
JP (2) JP2021533760A (es)
KR (1) KR20210043618A (es)
CN (2) CN119776468A (es)
AR (1) AR114548A1 (es)
AU (1) AU2019319970A1 (es)
BR (1) BR112021002480A2 (es)
CA (1) CA3107038A1 (es)
IL (1) IL280553A (es)
MX (1) MX2021001521A (es)
SG (1) SG11202100756RA (es)
TW (2) TW202021985A (es)
WO (1) WO2020033827A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4352094A1 (en) 2021-06-07 2024-04-17 Amgen Inc. Using fucosidase to control afucosylation level of glycosylated proteins
KR102682066B1 (ko) 2021-10-12 2024-07-05 프레스티지바이오로직스 주식회사 항체 집단의 제조 방법
CN115651953A (zh) * 2022-11-17 2023-01-31 康日百奥生物科技(苏州)有限公司 抗体药物、制备方法及细胞培养基
JP2025064615A (ja) * 2023-10-06 2025-04-17 住友化学株式会社 細胞接触用溶液又はそのゲル
WO2025160161A1 (en) * 2024-01-23 2025-07-31 Amgen Inc. Methods for modulating monoclonal antibody charge variants

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL85035A0 (en) 1987-01-08 1988-06-30 Int Genetic Eng Polynucleotide molecule,a chimeric antibody with specificity for human b cell surface antigen,a process for the preparation and methods utilizing the same
JP4124480B2 (ja) 1991-06-14 2008-07-23 ジェネンテック・インコーポレーテッド 免疫グロブリン変異体
ATE375365T1 (de) 1998-04-02 2007-10-15 Genentech Inc Antikörper varianten und fragmente davon
US6194551B1 (en) 1998-04-02 2001-02-27 Genentech, Inc. Polypeptide variants
AU2005233387B2 (en) * 2004-04-15 2011-05-26 Glycofi, Inc. Production of galactosylated glycoproteins in lower eukaryotes
JP5058483B2 (ja) * 2005-09-14 2012-10-24 オリンパス株式会社 生体試料の長期間ないし連続的検出方法
BRPI0810471A2 (pt) * 2007-04-16 2014-11-11 Momenta Pharmaceuticals Inc Produtos de glicoproteína definidos e métodos relacionados
KR20110139292A (ko) * 2009-04-20 2011-12-28 화이자 인코포레이티드 단백질 글리코실화의 제어 및 그와 관련된 조성물 및 방법
WO2011019622A1 (en) * 2009-08-14 2011-02-17 Genentech, Inc. Cell culture methods to make antibodies with enhanced adcc function
EP2511293A1 (en) * 2011-04-13 2012-10-17 LEK Pharmaceuticals d.d. A method for controlling the main complex N-glycan structures and the acidic variants and variability in bioprocesses producing recombinant proteins
WO2012149197A2 (en) * 2011-04-27 2012-11-01 Abbott Laboratories Methods for controlling the galactosylation profile of recombinantly-expressed proteins
US9493744B2 (en) 2012-06-20 2016-11-15 Genentech, Inc. Methods for viral inactivation and other adventitious agents
WO2014170866A2 (en) * 2013-04-18 2014-10-23 Dr. Reddy's Laboratories Limited Process of obtaining glycoprotein composition with increased galactosylation content
WO2015011660A1 (en) * 2013-07-23 2015-01-29 Biocon Limited Methods for controlling fucosylation levels in proteins
MX380800B (es) * 2014-01-29 2025-03-12 Lg Chemical Ltd Método de modulación de la galactosilación de proteína recombinante mediante optimización de medio de cultivo.
US20160280767A1 (en) * 2015-03-23 2016-09-29 Lonza Ltd. Methods for controlling protein glycosylation
HU231463B1 (hu) * 2015-08-04 2024-01-28 Richter Gedeon Nyrt. Módszer rekombináns proteinek galaktóz tartalmának növelésére

Also Published As

Publication number Publication date
SG11202100756RA (en) 2021-02-25
JP2025037936A (ja) 2025-03-18
WO2020033827A1 (en) 2020-02-13
BR112021002480A2 (pt) 2021-07-27
JP2021533760A (ja) 2021-12-09
CN119776468A (zh) 2025-04-08
TW202021985A (zh) 2020-06-16
CN112752769B (zh) 2024-12-10
TW202544040A (zh) 2025-11-16
MX2021001521A (es) 2021-04-19
EP3833688A1 (en) 2021-06-16
KR20210043618A (ko) 2021-04-21
IL280553A (en) 2021-03-25
AU2019319970A1 (en) 2021-03-11
US20210238644A1 (en) 2021-08-05
CN112752769A (zh) 2021-05-04
CA3107038A1 (en) 2020-02-13

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