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AR107260A1 - A TRANSGENIC SOYBEAN RESISTANT TO GLYPHOSATE AND THE SAME PREPARATION METHOD - Google Patents

A TRANSGENIC SOYBEAN RESISTANT TO GLYPHOSATE AND THE SAME PREPARATION METHOD

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Publication number
AR107260A1
AR107260A1 ARP160104073A ARP160104073A AR107260A1 AR 107260 A1 AR107260 A1 AR 107260A1 AR P160104073 A ARP160104073 A AR P160104073A AR P160104073 A ARP160104073 A AR P160104073A AR 107260 A1 AR107260 A1 AR 107260A1
Authority
AR
Argentina
Prior art keywords
glyphosate
transgenic
transgenic soybeans
dna
same preparation
Prior art date
Application number
ARP160104073A
Other languages
Spanish (es)
Inventor
Hui Yang
Shuang Wang
Longguo Jin
Huilong Hong
Lijuan Zhang
Yong Guo
Bingfu Guo
Lijuan Qiu
Original Assignee
Inst Of Crop Science Chinese Academy Of Agriculture Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Of Crop Science Chinese Academy Of Agriculture Sciences filed Critical Inst Of Crop Science Chinese Academy Of Agriculture Sciences
Publication of AR107260A1 publication Critical patent/AR107260A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C12N9/1092—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01019—3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente describe una soja transgénica resistente al glifosato y su método de preparación y aplicación. Proporciona un método para el fitomejoramiento de la soja transgénica, que comprende: insertar el fragmento de ADN exógeno en las posiciones 50, 543, 767 - 50, 543, 792 del cromosoma 19 del genoma de soja blanco para obtener la soja transgénica. La soja transgénica divulgada tiene tolerancia a la dosis alta de glifosato, y también se puede mejorar mediante la hibridación con una cepa de soja excelente para optimizar otros caracteres agronómicos tales como rendimiento, calidad etc. El método de detección de la presente divulgación puede identificar la región de unión del ADN-T insertado y ADN del genoma de la planta y además identificar célula, tejido, órgano, producto, etc. de la soja transgénica y su descendiente.This describes a glyphosate resistant transgenic soy and its method of preparation and application. It provides a method for plant breeding of transgenic soybeans, which comprises: inserting the exogenous DNA fragment at positions 50, 543, 767-50, 543, 792 of chromosome 19 of the white soybean genome to obtain transgenic soybeans. The reported transgenic soybeans have high dose tolerance of glyphosate, and can also be improved by hybridization with an excellent soy strain to optimize other agronomic traits such as yield, quality etc. The detection method of the present disclosure can identify the region of attachment of the inserted T-DNA and DNA of the plant genome and also identify cell, tissue, organ, product, etc. of transgenic soybeans and their offspring.

ARP160104073A 2015-12-30 2016-12-29 A TRANSGENIC SOYBEAN RESISTANT TO GLYPHOSATE AND THE SAME PREPARATION METHOD AR107260A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201511022465.8A CN105505981B (en) 2015-12-30 2015-12-30 A kind of resistance glyphosate genetically engineered soybean and the preparation method and application thereof

Publications (1)

Publication Number Publication Date
AR107260A1 true AR107260A1 (en) 2018-04-11

Family

ID=55714274

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP160104073A AR107260A1 (en) 2015-12-30 2016-12-29 A TRANSGENIC SOYBEAN RESISTANT TO GLYPHOSATE AND THE SAME PREPARATION METHOD

Country Status (3)

Country Link
CN (1) CN105505981B (en)
AR (1) AR107260A1 (en)
WO (1) WO2017113573A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105505981B (en) * 2015-12-30 2018-12-25 中国农业科学院作物科学研究所 A kind of resistance glyphosate genetically engineered soybean and the preparation method and application thereof
CN106350532A (en) * 2016-08-28 2017-01-25 浙江大学 Glyphosate-resistant Fusion Gene, Encoding Protein and Application
CN108179146B (en) * 2018-02-03 2022-02-11 吉林省农业科学院 Disease-resistant transgenic soybean event B5C9120-3 exogenous insert flanking sequence and application thereof
CN108239639B (en) * 2018-02-03 2022-02-11 吉林省农业科学院 Flanking sequence of exogenous insert of stress-tolerant transgenic soybean event WB1 and application thereof
CN108179147B (en) * 2018-02-03 2022-02-11 吉林省农业科学院 Flanking Sequences of Foreign Insert Fragments of High Oleic Acid Transgenic Soybean Event E2D9050 and Its Application
CN112359123A (en) * 2020-10-10 2021-02-12 浙江省农业科学院 Real-time fluorescence quantitative detection method for g10evo-epsps transgenic soybean and kit thereof
CN112322631B (en) * 2020-11-06 2022-08-16 武汉天问生物科技有限公司 Cultivation method of glyphosate-resistant transgenic soybean
CN114807221B (en) * 2022-04-28 2023-06-06 宁波大学科学技术学院 Mutant, protein and expression vector of rice root elongation regulatory gene OsDRP1C and application thereof
CN118957138A (en) * 2024-07-08 2024-11-15 中国农业科学院生物技术研究所 A PCR detection kit and detection method for glyphosate-resistant transgenic soybeans

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101278053A (en) * 2005-07-29 2008-10-01 孟山都技术有限公司 Using segregants from transgenic hybrids to develop new germplasm
CN100365133C (en) * 2005-10-21 2008-01-30 天津师范大学 A detection method of transgenic soybean and primers used
CN101100676B (en) * 2007-06-15 2010-12-22 中国农业科学院生物技术研究所 Bivalent expression vectors for breeding glyphosate-resistant plants
TW201231655A (en) * 2010-12-15 2012-08-01 Syngenta Participations Ag Soybean event SYHT04R and compositions and methods for detection thereof
CN104450755B (en) * 2014-11-14 2017-07-11 重庆大学 The application of a kind of Asiatic migrotory locust atp synthase α subunit genes and its dsRNA in control of insect
CN105505981B (en) * 2015-12-30 2018-12-25 中国农业科学院作物科学研究所 A kind of resistance glyphosate genetically engineered soybean and the preparation method and application thereof

Also Published As

Publication number Publication date
CN105505981B (en) 2018-12-25
CN105505981A (en) 2016-04-20
WO2017113573A1 (en) 2017-07-06

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