AR104430A1 - Planta del maíz dbn9978 y secuencias de ácido nucleico y métodos para la detección de las mismas - Google Patents
Planta del maíz dbn9978 y secuencias de ácido nucleico y métodos para la detección de las mismasInfo
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- AR104430A1 AR104430A1 ARP160101199A ARP160101199A AR104430A1 AR 104430 A1 AR104430 A1 AR 104430A1 AR P160101199 A ARP160101199 A AR P160101199A AR P160101199 A ARP160101199 A AR P160101199A AR 104430 A1 AR104430 A1 AR 104430A1
- Authority
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- Argentina
- Prior art keywords
- seq
- nucleic acid
- acid sequence
- corn
- plant
- Prior art date
Links
- 150000007523 nucleic acids Chemical group 0.000 title abstract 20
- 241001057636 Dracaena deremensis Species 0.000 title abstract 15
- 238000000034 method Methods 0.000 title abstract 6
- 238000001514 detection method Methods 0.000 title 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 17
- 240000008042 Zea mays Species 0.000 abstract 12
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 abstract 12
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract 12
- 235000005822 corn Nutrition 0.000 abstract 12
- 239000005562 Glyphosate Substances 0.000 abstract 11
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 abstract 11
- 229940097068 glyphosate Drugs 0.000 abstract 11
- 230000000295 complement effect Effects 0.000 abstract 10
- 241000238631 Hexapoda Species 0.000 abstract 9
- 241000196324 Embryophyta Species 0.000 abstract 8
- 239000004009 herbicide Substances 0.000 abstract 7
- 230000002363 herbicidal effect Effects 0.000 abstract 6
- 239000002773 nucleotide Substances 0.000 abstract 6
- 125000003729 nucleotide group Chemical group 0.000 abstract 6
- 230000009261 transgenic effect Effects 0.000 abstract 6
- 108091093088 Amplicon Proteins 0.000 abstract 5
- 108010020183 3-phosphoshikimate 1-carboxyvinyltransferase Proteins 0.000 abstract 3
- 108020004414 DNA Proteins 0.000 abstract 2
- 230000003321 amplification Effects 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 230000008654 plant damage Effects 0.000 abstract 2
- 235000018102 proteins Nutrition 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 229920002261 Corn starch Polymers 0.000 abstract 1
- 108010068370 Glutens Proteins 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 235000005687 corn oil Nutrition 0.000 abstract 1
- 239000002285 corn oil Substances 0.000 abstract 1
- 239000008120 corn starch Substances 0.000 abstract 1
- 239000002537 cosmetic Substances 0.000 abstract 1
- 230000037213 diet Effects 0.000 abstract 1
- 235000005911 diet Nutrition 0.000 abstract 1
- 230000037406 food intake Effects 0.000 abstract 1
- 235000021312 gluten Nutrition 0.000 abstract 1
- 230000009545 invasion Effects 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
- 239000002994 raw material Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
- C12N15/8275—Glyphosate
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
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- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
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- Organic Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
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- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Forests & Forestry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Cell Biology (AREA)
- Environmental Sciences (AREA)
- Ecology (AREA)
- Analytical Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Botany (AREA)
- Agronomy & Crop Science (AREA)
- Immunology (AREA)
- Dentistry (AREA)
- Insects & Arthropods (AREA)
- Mycology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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Abstract
Reivindicación 1: Una secuencia de ácido nucleico, caracterizada porque comprende al menos 11 nucleótidos consecutivos de SEQ ID Nº 3 o una secuencia complementaria de la misma, y/o al menos 11 nucleótidos consecutivos de SEQ ID Nº 4 o una secuencia complementaria de la misma. Reivindicación 5: Un método para detectar la presencia del ADN del evento de maíz transgénico DBN9978 en una muestra, que comprende: poner en contacto la muestra con al menos dos cebadores en una reacción de amplificación de ácido nucleico; realizar la reacción de amplificación de ácido nucleico; y detectar la presencia de un amplicón, en el que la presencia del amplicón indica la presencia del ADN del evento de maíz transgénico DBN9978 en la muestra; en el que el amplicón comprende al menos 11 nucleótidos consecutivos de la SEQ ID Nº 3 o una secuencia complementaria de la misma, o al menos 11 nucleótidos consecutivos de la SEQ ID Nº 4 o una secuencia complementaria de la misma; y preferiblemente, el amplicón comprende los nucleótidos consecutivos 1 - 11 ó 12 - 22 de la SEQ ID Nº 1 o una secuencia complementaria de la misma, o los nucleótidos consecutivos 1 - 11 ó 12 - 22 de la SEQ ID Nº 2 o una secuencia complementaria de la misma; preferiblemente, el amplicón comprende la SEQ ID Nº 1 o una secuencia complementaria de la misma, SEQ ID Nº 2 o una secuencia complementaria de la misma, SEQ ID Nº 6 o una secuencia complementaria de la misma, o SEQ ID Nº 7 o una secuencia complementaria de la misma. Reivindicación 11: Una célula o parte de planta, caracterizada porque comprende una secuencia de ácido nucleico que codifica la proteína Cry1Ab resistente a los insectos, una secuencia de ácido nucleico que codifica la proteína EPSPS tolerante a herbicidas de glifosato y una secuencia de ácido nucleico de una región específica, en el que la secuencia de ácido nucleico de una región específica comprende la SEQ ID Nº 1, SEQ ID Nº 2, SEQ ID Nº 6 o SEQ ID Nº 7; preferiblemente, la célula o parte de la planta de maíz comprende una secuencia de ácido nucleico que codifica la proteína Cry1Ab resistente a los insectos, una secuencia de ácido nucleico que codifica la proteína EPSPS de tolerancia a herbicidas de glifosato y una secuencia de ácido nucleico de una región específica, en el que la secuencia de ácido nucleico de una región específica comprende la SEQ ID Nº 3 o SEQ ID Nº 4; preferiblemente, la célula o parte de la planta de maíz comprende la secuencia de ácido nucleico tal como se establece en la SEQ ID Nº 5. Reivindicación 12: Un método para proteger una planta de maíz de la invasión de insectos, caracterizado porque comprende proporcionar al menos una célula o parte de la planta de maíz transgénico de la reivindicación 11 en la dieta del insecto diana, y la ingestión de la célula o parte de la planta de maíz transgénico inhibe al insecto diana de alimentarse aún más de la planta de maíz. Reivindicación 15: Un método para cultivar una planta de maíz tolerante a herbicidas de glifosato, caracterizado porque comprende: plantar al menos una semilla de maíz, en el que la semilla de maíz comprende en su genoma una secuencia de ácido nucleico que codifica la proteína EPSPS tolerante a los herbicidas de glifosato y una secuencia de ácido nucleico de una región específica; crecer la semilla de maíz en una planta de maíz; y pulverizar la planta de maíz con una cantidad eficaz de herbicida de glifosato, y a continuación recoger la planta con una reducción de daños en la planta, en comparación con otras plantas que no comprenden la secuencia de ácido nucleico de la región específica; en el que la secuencia de ácido nucleico de la región específica se selecciona entre al menos una secuencia de ácido nucleico que consiste en la SEQ ID Nº 1, SEQ ID Nº 2, SEQ ID Nº 3, SEQ ID Nº 4, SEQ ID Nº 6 y SEQ ID Nº 7; preferiblemente, el método comprende: plantar al menos una semilla de maíz, en el que la semilla de maíz comprende en su genoma la secuencia de ácido nucleico tal como se establece en la SEQ ID Nº 5; crecer la semilla de maíz en una planta de maíz; y pulverizar la planta de maíz con una cantidad eficaz de herbicida de glifosato, y a continuación recoger la planta con una reducción de daños en la planta, en comparación con otras plantas que no comprenden la SEQ ID Nº 5. Reivindicación 19: Un método para producir una planta de maíz resistente a los insectos y tolerante a herbicidas de glifosato, caracterizado porque comprende cruzar sexualmente una primera planta de maíz parental tolerante a herbicidas de glifosato y resistente a los insectos del evento de maíz transgénicoDBN9978, con una segunda planta de maíz parental que carece de la característica de tolerancia a glifosato y/o resistencia a los insectos, produciendo de ese modo una pluralidad de plantas de la progenie; tratar las plantas de la progenie con herbicida de glifosato; y seleccionar las plantas de la progenie tolerantes a glifosato, que también son resistentes al daño por alimentación de un insecto; en el que el evento de maíz transgénico DBN9978 comprende en su genoma al menos una secuencia de ácido nucleico seleccionada del grupo que consiste en la SEQ ID Nº 1, SEQ ID Nº 2, SEQ ID Nº 3, SEQ ID Nº 4, SEQ ID Nº 5, SEQ ID Nº 6 y SEQ ID Nº 7. Reivindicación 21: Un producto o materia prima agrícola que comprende los polinucleótidos tal come se establecen en la SEQ ID Nº 1 o SEQ ID Nº 2, caracterizado porque el producto o materia agrícola es polenta, harina de maíz, aceite de maíz, almidón de maíz, gluten de maíz, tortas de maíz, un cosmético o un agente de carga.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510218637.2A CN104878091B (zh) | 2015-04-30 | 2015-04-30 | 用于检测玉米植物dbn9978的核酸序列及其检测方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR104430A1 true AR104430A1 (es) | 2017-07-19 |
Family
ID=53945765
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP160101199A AR104430A1 (es) | 2015-04-30 | 2016-04-28 | Planta del maíz dbn9978 y secuencias de ácido nucleico y métodos para la detección de las mismas |
Country Status (3)
| Country | Link |
|---|---|
| CN (1) | CN104878091B (es) |
| AR (1) | AR104430A1 (es) |
| WO (1) | WO2016173362A1 (es) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104878091B (zh) * | 2015-04-30 | 2019-05-17 | 北京大北农科技集团股份有限公司 | 用于检测玉米植物dbn9978的核酸序列及其检测方法 |
| CN109971880B (zh) * | 2019-04-09 | 2022-11-04 | 北京大北农生物技术有限公司 | 用于检测玉米植物dbn9508的核酸序列及其检测方法 |
| CN112280743B (zh) * | 2020-11-04 | 2023-06-02 | 北京粮元生物科技有限公司 | 玉米事件2a-7及其鉴定方法 |
| US12534768B2 (en) | 2020-11-04 | 2026-01-27 | Beijing Grainsmeta Biotech Co., LTD | Corn event 2A-7 and identification method therefor |
| CN113151533B (zh) * | 2021-01-27 | 2023-08-08 | 隆平生物技术(海南)有限公司 | 转基因玉米事件lp007-6及其检测方法 |
| CN112852991B (zh) * | 2021-01-27 | 2023-08-04 | 隆平生物技术(海南)有限公司 | 转基因玉米事件lp007-7及其检测方法 |
| CN112831585B (zh) * | 2021-01-27 | 2023-06-16 | 隆平生物技术(海南)有限公司 | 转基因玉米事件lp007-4及其检测方法 |
| CN112852801B (zh) * | 2021-01-27 | 2023-08-08 | 隆平生物技术(海南)有限公司 | 转基因玉米事件lp007-1及其检测方法 |
| CN113151534B (zh) * | 2021-01-27 | 2023-06-20 | 隆平生物技术(海南)有限公司 | 转基因玉米事件lp007-5及其检测方法 |
| CN113980958B (zh) * | 2021-10-12 | 2023-08-11 | 隆平生物技术(海南)有限公司 | 转基因玉米事件lp007-8及其检测方法 |
| CN116144818B (zh) * | 2022-09-23 | 2023-11-07 | 隆平生物技术(海南)有限公司 | 转基因玉米事件lp026-2及其检测方法 |
| CN116144672B (zh) * | 2022-09-23 | 2023-11-07 | 隆平生物技术(海南)有限公司 | 转基因玉米事件lp026-1及其检测方法 |
| CN119530421A (zh) * | 2023-08-28 | 2025-02-28 | 青岛清原种子科学有限公司 | 转基因玉米事件qy2569-42及其检测方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6593273B2 (en) * | 2000-10-06 | 2003-07-15 | Monsanto Technology Llc | Method for reducing pest damage to corn by treating transgenic corn seeds with pesticide |
| RS54170B1 (sr) * | 2003-12-15 | 2015-12-31 | Monsanto Technology, Llc | Biljka kukuruza mon88017 i kompozicije i postupci za detekciju |
| AU2007267586B2 (en) * | 2006-05-26 | 2012-04-05 | Monsanto Technology, Llc | Corn plant and seed corresponding to transgenic event MON89034 and methods for detection and use thereof |
| US8012714B2 (en) * | 2008-04-14 | 2011-09-06 | Innovative Targeting Solutions, Inc. | Sequence diversity generation in immunoglobulins |
| JP2015516143A (ja) * | 2012-04-02 | 2015-06-08 | モデルナ セラピューティクス インコーポレイテッドModerna Therapeutics,Inc. | ヒト疾患に関連するタンパク質の産生のための修飾ポリヌクレオチド |
| WO2013169923A2 (en) * | 2012-05-08 | 2013-11-14 | Monsanto Technology Llc | Corn event mon 87411 |
| CN104878091B (zh) * | 2015-04-30 | 2019-05-17 | 北京大北农科技集团股份有限公司 | 用于检测玉米植物dbn9978的核酸序列及其检测方法 |
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2015
- 2015-04-30 CN CN201510218637.2A patent/CN104878091B/zh active Active
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2016
- 2016-03-30 WO PCT/CN2016/077867 patent/WO2016173362A1/zh not_active Ceased
- 2016-04-28 AR ARP160101199A patent/AR104430A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CN104878091A (zh) | 2015-09-02 |
| CN104878091B (zh) | 2019-05-17 |
| WO2016173362A1 (zh) | 2016-11-03 |
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