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AR104170A1 - PLANTS WITH MALE STERILITY OF THE TRITICUM SPECIES - Google Patents

PLANTS WITH MALE STERILITY OF THE TRITICUM SPECIES

Info

Publication number
AR104170A1
AR104170A1 ARP160100889A ARP160100889A AR104170A1 AR 104170 A1 AR104170 A1 AR 104170A1 AR P160100889 A ARP160100889 A AR P160100889A AR P160100889 A ARP160100889 A AR P160100889A AR 104170 A1 AR104170 A1 AR 104170A1
Authority
AR
Argentina
Prior art keywords
nucleotide sequence
seq
tdf
protein
amino acid
Prior art date
Application number
ARP160100889A
Other languages
Spanish (es)
Original Assignee
Kws Saat Se & Co Kgaa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kws Saat Se & Co Kgaa filed Critical Kws Saat Se & Co Kgaa
Publication of AR104170A1 publication Critical patent/AR104170A1/en

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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823Reproductive tissue-specific promoters
    • C12N15/8231Male-specific, e.g. anther, tapetum, pollen
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/13Plant traits
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Botany (AREA)
  • Reproductive Health (AREA)
  • Pregnancy & Childbirth (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente se refiere a una planta con esterilidad masculina de la especie Triticum, la totalidad de cuyos alelos del gen que codifica TDF (tapetal development and function) están desactivados, y a sus partes, semillas y descendientes, como también a vectores y plantas transgénicas, procedimientos para la producción de plantas, procedimientos para restaurar una fertilidad, y procedimientos para la comprobación de esterilidad masculina. Reivindicación 2: Planta de acuerdo con la reivindicación 1, caracterizada porque el gen que codifica TDF comprende una secuencia de nucleótidos seleccionada entre las siguientes: (a) una secuencia de nucleótidos de la SEQ ID Nº 1, 2 ó 3 o de la SEQ ID Nº 7, 8 ó 9; (b) una secuencia de nucleótidos, que codifica una proteína que contiene una de las secuencias de aminoácidos de la SEQ ID Nº 10, 11 ó 12 o una secuencia de aminoácidos que presenta una identidad de secuencias de por lo menos el 80% con respecto a una de las secuencias de aminoácidos de la SEQ ID Nº 10, 11 ó 12; (c) una secuencia de nucleótidos, que es complementaria con respecto a (a) o (b); (d) una secuencia de nucleótidos, que es homóloga con respecto a (a) o (b), en donde la secuencia de nucleótidos codifica una proteína de TDF; (e) una secuencia de nucleótidos, que en condiciones rigurosas se hibrida con (a) o (b); y (f) una secuencia de nucleótidos, que en condiciones rigurosas se hibrida con (c), en donde la secuencia de nucleótidos codifica una proteína de TDF. Reivindicación 11: Ácido nucleico que comprende una secuencia de nucleótidos seleccionada entre las siguientes: (a) una secuencia de nucleótidos de la SEQ ID Nº 1, 2 ó 3 o de la SEQ ID Nº 7, 8 ó 9; (b) una secuencia de nucleótidos, que codifica una proteína que contiene una de las secuencias de aminoácidos de la SEQ ID Nº 10, 11 ó 12 o una secuencia de aminoácidos que presenta una identidad de por lo menos el 80% con respecto a una de las secuencias de aminoácidos de la SEQ ID Nº 10, 11 ó 12; (c) una secuencia de nucleótidos, que es complementaria con respecto a (a) o (b); (d) una secuencia de nucleótidos, que es homóloga con respecto a (a) o (b), en donde la secuencia de nucleótidos codifica una proteína de TDF; (e) una secuencia de nucleótidos, que en condiciones rigurosas se hibrida con (a) o (b); (f) una secuencia de nucleótidos, que en condiciones rigurosas se hibrida con (c), en donde la secuencia de nucleótidos codifica una proteína de TDF; (g) una secuencia de nucleótidos, que es una secuencia parcial de las secuencias de nucleótidos mencionadas en de (a) a (f) con por lo menos 17 nucleótidos consecutivos en una orientación de sentido, de antisentido o complementaria.This refers to a plant with male sterility of the species Triticum, all of whose alleles of the gene coding for TDF (tapetal development and function) are deactivated, and their parts, seeds and descendants, as well as vectors and transgenic plants, procedures for the production of plants, procedures for restoring fertility, and procedures for checking male sterility. Claim 2: Plant according to claim 1, characterized in that the gene encoding TDF comprises a nucleotide sequence selected from the following: (a) a nucleotide sequence of SEQ ID No. 1, 2 or 3 or SEQ ID No. 7, 8 or 9; (b) a nucleotide sequence, which encodes a protein that contains one of the amino acid sequences of SEQ ID No. 10, 11 or 12 or an amino acid sequence that has a sequence identity of at least 80% with respect to to one of the amino acid sequences of SEQ ID No. 10, 11 or 12; (c) a nucleotide sequence, which is complementary with respect to (a) or (b); (d) a nucleotide sequence, which is homologous with respect to (a) or (b), wherein the nucleotide sequence encodes a TDF protein; (e) a nucleotide sequence, which under stringent conditions hybridizes with (a) or (b); and (f) a nucleotide sequence, which under stringent conditions hybridizes with (c), wherein the nucleotide sequence encodes a TDF protein. Claim 11: Nucleic acid comprising a nucleotide sequence selected from the following: (a) a nucleotide sequence of SEQ ID No. 1, 2 or 3 or of SEQ ID No. 7, 8 or 9; (b) a nucleotide sequence, which encodes a protein that contains one of the amino acid sequences of SEQ ID No. 10, 11 or 12 or an amino acid sequence that has an identity of at least 80% with respect to a of the amino acid sequences of SEQ ID No. 10, 11 or 12; (c) a nucleotide sequence, which is complementary with respect to (a) or (b); (d) a nucleotide sequence, which is homologous with respect to (a) or (b), wherein the nucleotide sequence encodes a TDF protein; (e) a nucleotide sequence, which under stringent conditions hybridizes with (a) or (b); (f) a nucleotide sequence, which under stringent conditions hybridizes with (c), wherein the nucleotide sequence encodes a TDF protein; (g) a nucleotide sequence, which is a partial sequence of the nucleotide sequences mentioned in from (a) to (f) with at least 17 consecutive nucleotides in a sense, antisense or complementary orientation.

ARP160100889A 2015-04-02 2016-04-01 PLANTS WITH MALE STERILITY OF THE TRITICUM SPECIES AR104170A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102015004187.8A DE102015004187A1 (en) 2015-04-02 2015-04-02 Male sterile plant of the genus Triticum

Publications (1)

Publication Number Publication Date
AR104170A1 true AR104170A1 (en) 2017-06-28

Family

ID=55809069

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP160100889A AR104170A1 (en) 2015-04-02 2016-04-01 PLANTS WITH MALE STERILITY OF THE TRITICUM SPECIES

Country Status (3)

Country Link
AR (1) AR104170A1 (en)
DE (1) DE102015004187A1 (en)
WO (1) WO2016156583A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4219730A1 (en) 2015-01-27 2023-08-02 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Method for conducting site-specific modification on entire plant via gene transient expression
KR102194612B1 (en) 2015-03-16 2020-12-23 인스티튜트 오브 제네틱스 앤드 디벨롭멘털 바이오롤지, 차이니즈 아카데미 오브 사이언시스 Site-specific modification method of plant genome using non-genetic material
EP3095870A1 (en) 2015-05-19 2016-11-23 Kws Saat Se Methods for the in planta transformation of plants and manufacturing processes and products based and obtainable therefrom
JP2018527920A (en) 2015-08-14 2018-09-27 インスティテュート・オブ・ジェネティクス・アンド・ディヴェロプメンタル・バイオロジー、チャイニーズ・アカデミー・オブ・サイエンシズInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences Method for obtaining glyphosate-tolerant rice by site-specific nucleotide substitution
EP3301111A1 (en) * 2016-10-02 2018-04-04 Kws Saat Se Plant of the genus triticum, in which the tdf-gene is inactivated by a marker gene

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7612251B2 (en) 2000-09-26 2009-11-03 Pioneer Hi-Bred International, Inc. Nucleotide sequences mediating male fertility and method of using same
EP2241624A3 (en) * 2004-06-15 2011-02-09 Latrobe University Nucleic acid molecules and their use in plant sterility
US9803214B2 (en) * 2013-03-12 2017-10-31 Pioneer Hi-Bred International, Inc. Breeding pair of wheat plants comprising an MS45 promoter inverted repeat that confers male sterility and a construct that restores fertility

Also Published As

Publication number Publication date
DE102015004187A1 (en) 2016-10-06
WO2016156583A1 (en) 2016-10-06

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