[go: up one dir, main page]

AR099024A1 - Expresión específica de tejido y producción de planta híbrida - Google Patents

Expresión específica de tejido y producción de planta híbrida

Info

Publication number
AR099024A1
AR099024A1 ARP150100006A ARP150100006A AR099024A1 AR 099024 A1 AR099024 A1 AR 099024A1 AR P150100006 A ARP150100006 A AR P150100006A AR P150100006 A ARP150100006 A AR P150100006A AR 099024 A1 AR099024 A1 AR 099024A1
Authority
AR
Argentina
Prior art keywords
rnas
tissue
plant cell
specific
plant
Prior art date
Application number
ARP150100006A
Other languages
English (en)
Inventor
A German Marcelo
Wright Terry
Kumar Sandeep
Coram Tristan
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of AR099024A1 publication Critical patent/AR099024A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8217—Gene switch
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8231—Male-specific, e.g. anther, tapetum, pollen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Esta descripción se refiere al uso de la maquinaria de ARNi de planta endógena para reducir en forma preferencial o especifica de la expresión del transgén. En algunas formas de realización, la descripción trata la reducción específica de la expresión del transgén en los tejidos de planta masculinos, por ejemplo, para proporcionar un sistema de esterilidad masculina económica de la producción de semilla híbrida. Reivindicación 8: Un método para producir una célula de planta transgénica, el método que comprende: proporcionar un ácido nucleico que comprende un gen de resistencia al herbicida, donde el gen de resistencia al herbicida comprende un sitio blanco de un ARN pequeño (ARNs); y transformar una célula de planta con el ácido nucleico, donde la célula de planta expresa el ARNs. Reivindicación 10: El método de acuerdo con la reivindicación 8, donde el ARNs endógeno que se expresa en la célula de planta de una manera preferida de tejido, especifica de tejido, especifico de tipo celular, o inducible. Reivindicación 11: El método de acuerdo con la reivindicación 10, donde el ARNs es miR529.
ARP150100006A 2013-12-31 2015-01-05 Expresión específica de tejido y producción de planta híbrida AR099024A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201361922603P 2013-12-31 2013-12-31

Publications (1)

Publication Number Publication Date
AR099024A1 true AR099024A1 (es) 2016-06-22

Family

ID=53481049

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP150100006A AR099024A1 (es) 2013-12-31 2015-01-05 Expresión específica de tejido y producción de planta híbrida

Country Status (7)

Country Link
US (3) US10683513B2 (es)
EP (1) EP3090050B1 (es)
CN (2) CN110172472B (es)
AR (1) AR099024A1 (es)
BR (1) BR112016015059A2 (es)
CA (1) CA2934959C (es)
WO (1) WO2015103417A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10683513B2 (en) * 2013-12-31 2020-06-16 Dow Agrosciences Llc Tissue-specific expression and hybrid plant production
CN110092819B (zh) * 2018-11-13 2021-07-16 中国农业大学 玉米苞叶宽度调控蛋白arf2及其编码基因与应用
CN110218810B (zh) * 2019-05-05 2022-06-03 华南农业大学 调控玉米雄穗构型的启动子、分子标记及其应用
BR112022014311A2 (pt) * 2020-01-21 2022-09-20 Pioneer Hi Bred Int Direcionamento de microrna para regular a função de gene nativo por edição genômica
CN112328966B (zh) * 2020-10-26 2024-08-13 北京市华都峪口禽业有限责任公司 畜禽配套系中纯系对商品代性状影响大小的确定方法
CN115948458A (zh) * 2022-09-21 2023-04-11 深圳大学 一种调控玉米雄穗分枝数的方法

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4535060A (en) 1983-01-05 1985-08-13 Calgene, Inc. Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use
NL8300698A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
US4940835A (en) 1985-10-29 1990-07-10 Monsanto Company Glyphosate-resistant plants
US4810648A (en) 1986-01-08 1989-03-07 Rhone Poulenc Agrochimie Haloarylnitrile degrading gene, its use, and cells containing the gene
ES2018274T5 (es) 1986-03-11 1996-12-16 Plant Genetic Systems Nv Celulas vegetales resistentes a los inhibidores de glutamina sintetasa, preparadas por ingenieria genetica.
US4975374A (en) 1986-03-18 1990-12-04 The General Hospital Corporation Expression of wild type and mutant glutamine synthetase in foreign hosts
EP0333033A1 (en) 1988-03-09 1989-09-20 Meiji Seika Kaisha Ltd. Glutamine synthesis gene and glutamine synthetase
CA1339684C (en) 1988-05-17 1998-02-24 Peter H. Quail Plant ubquitin promoter system
AU5664090A (en) 1989-05-05 1990-11-29 Biosource Genetics Corporation Male sterility in plants
US5658772A (en) 1989-12-22 1997-08-19 E. I. Du Pont De Nemours And Company Site-specific recombination of DNA in plant cells
US5633435A (en) 1990-08-31 1997-05-27 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
WO1994000977A1 (fr) 1992-07-07 1994-01-20 Japan Tobacco Inc. Procede de transformation d'une monocotyledone
US5659026A (en) 1995-03-24 1997-08-19 Pioneer Hi-Bred International ALS3 promoter
RU2129005C1 (ru) * 1996-05-21 1999-04-20 Михеева Элеонора Федоровна Сбор для восстановления функции щитовидной железы
US5994123A (en) 1996-08-09 1999-11-30 Regents Of University Of Minnesota Sugarcane bacilliform virus promoter
CA2293738A1 (en) 1997-06-12 1998-12-17 Dow Agrosciences Llc Regulatory sequences for transgenic plants
US6720475B1 (en) 1997-11-18 2004-04-13 Pioneer Hi-Bred International, Inc. Modified nucleic acid sequence encoding FLP recombinase
US6271439B1 (en) 1998-03-04 2001-08-07 Pioneer Hi-Bred International, Inc. Methods and compositions for regulating cell death and enhancing disease resistance to plant pathogens
CA2490274A1 (en) 2002-06-27 2004-01-08 Dow Agrosciences Llc Use of regulatory sequences in transgenic plants
CN1863914B (zh) 2003-04-29 2011-03-09 先锋高级育种国际公司 新的草甘膦-n-乙酰转移酶(gat)基因
PL2308976T5 (pl) 2004-04-30 2017-10-31 Dow Agrosciences Llc Nowe geny odporności na herbicydy
EP1765058A4 (en) 2004-06-15 2008-05-28 Univ Latrobe NUCLEIC ACID MOLECULES AND THEIR USE FOR POLLENSTERILITY IN PLANTS
US8314290B2 (en) 2004-12-21 2012-11-20 Monsanto Technology Llc Temporal regulation of gene expression by MicroRNAs
EP1824967B1 (en) * 2004-12-21 2014-11-05 Monsanto Technology, LLC Recombinant dna constructs and methods for controlling gene expression
EP1838144B1 (en) * 2005-01-07 2016-08-31 Oregon State University Method to trigger rna interference
CN101203611B (zh) 2005-04-19 2013-08-14 巴斯福植物科学有限公司 控制基因表达的改良方法
ZA200803234B (en) 2005-10-13 2009-01-28 Monsanto Technology Llc Methods for producing hybrid seed
WO2007047016A2 (en) 2005-10-13 2007-04-26 Monsanto Technology, Llc Methods for producing hybrid seed
MX363711B (es) 2005-10-28 2019-03-29 Dow Agrosciences Llc Genes novedosos para resistencia a herbicida.
EP3159414A1 (en) * 2006-02-10 2017-04-26 Oxitec Limited Expression system
KR101439568B1 (ko) 2006-08-11 2014-09-12 다우 아그로사이언시즈 엘엘씨 아연 손가락 뉴클레아제-매개 상동 재조합
US8946511B2 (en) * 2006-10-12 2015-02-03 Monsanto Technology Llc Plant microRNAs and methods of use thereof
TWI470079B (zh) 2006-12-14 2015-01-21 Dow Agrosciences Llc 最佳化之非標準鋅指蛋白
CN101889090B (zh) 2007-10-05 2018-01-23 陶氏益农公司 用于将分子物质转移入植物细胞中的方法
WO2009100188A2 (en) 2008-02-08 2009-08-13 Dow Agrosciences Llc Methods for detection of corn event das-59132
ES2563485T3 (es) * 2008-07-01 2016-03-15 Monsanto Technology Llc Construcciones de ADN recombinante y procedimientos para modular la expresión de un gen diana
CA2746892C (en) * 2008-12-24 2019-05-28 National Research Council Of Canada Tor-interacting proteins (tips) and genes therefor
WO2011067745A2 (en) 2009-12-06 2011-06-09 Rosetta Green Ltd. Compositions and methods for enhancing plants resistance to abiotic stress
CN102304520A (zh) * 2010-01-26 2012-01-04 中国科学院遗传与发育生物学研究所 小分子rna的调控位点及其应用
RU2611188C2 (ru) 2010-07-29 2017-02-21 ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи Штаммы agrobacterium, модифицированные для увеличения частоты трансформации растений
US9222100B2 (en) 2010-08-24 2015-12-29 Monsanto Technology Llc Methods and DNA constructs for autoregulating transgene silencing
MX354471B (es) * 2011-07-01 2018-03-07 Monsanto Technology Llc Métodos y composiciones para la regulación selectiva de la expresión de proteínas.
WO2013184768A1 (en) 2012-06-05 2013-12-12 University Of Georgia Research Foundation, Inc. Compositions and methods of gene silencing in plants
US10683513B2 (en) * 2013-12-31 2020-06-16 Dow Agrosciences Llc Tissue-specific expression and hybrid plant production

Also Published As

Publication number Publication date
US10913953B2 (en) 2021-02-09
CA2934959A1 (en) 2015-07-09
CN110172472B (zh) 2024-06-14
EP3090050B1 (en) 2021-01-20
EP3090050A4 (en) 2017-10-11
US20180346916A1 (en) 2018-12-06
BR112016015059A2 (pt) 2017-09-19
CN106170548B (zh) 2019-04-19
US20210171968A1 (en) 2021-06-10
EP3090050A1 (en) 2016-11-09
CA2934959C (en) 2023-12-05
WO2015103417A9 (en) 2015-12-03
WO2015103417A1 (en) 2015-07-09
CN110172472A (zh) 2019-08-27
US20150184178A1 (en) 2015-07-02
CN106170548A (zh) 2016-11-30
US10683513B2 (en) 2020-06-16

Similar Documents

Publication Publication Date Title
AR099024A1 (es) Expresión específica de tejido y producción de planta híbrida
CL2017000882A1 (es) Moléculas de ácido nucleico de la subunidad alfa del coatómero copi que confiere resistencia contra plagas de coleópteros y hemípteros
MX350966B (es) Celulas madre pluripotentes.
CO2017007335A2 (es) Supresión del gen de la huntingtina inducida por la arni
CY1120808T1 (el) Μεθοδοι και συνθεσεις για στοχευμενες γενετικες τροποποιησεις και μεθοδοι χρησης
CL2012002474A1 (es) Metodo para regular la expresion de un gen endogeno objetivo en una planta u organos vegetales en crecimiento que comprende al menos un polinucleotido que tiene una secuencia esencialmente identica a una secuencia de 18 o mas nucleotidos contiguos en dicho gen endogeno o en el arn mensajero transcrito a partir de dicho gen
MX2019015525A (es) Modificacion genetica de ratas.
MX2016007654A (es) Metodos y composiciones para la modificacion dirigida de un genoma.
BR112014026186A2 (pt) método de transfecção celular
CO6731143A2 (es) Moléculas de ácido nucleico que suministran resistencia contra las plagas de los coleópteros
AR091482A1 (es) Celulas modificadas geneticamente y metodos par su obtencion
CL2018001577A1 (es) Anticuerpos anti-c5 y métodos de uso
AR065243A1 (es) Composiciones y metodos que utilizan arn de interferencia para el control de nematodos
AR088262A1 (es) Moleculas de acido nucleico que se dirigen a rpa70 y confieren resistencia a plagas de coleopteros
CO2017004735A2 (es) Moléculas que tienen utilidad plaguicida e intermediarios, composiciones y procedimientos relacionados con las mismas
CO2017006663A2 (es) Supresión de iarn parental de genes de remodelación de cromatina para controlar plagas de coleópteros
CL2017001519A1 (es) Supresión de arni parental de gen hunchback para el control de las plagas de hemípteros
MX2016008263A (es) Moleculas de acido nucleico rnapii 140 que confieren resistencia a plagas de coleopteros.
CO2017009163A2 (es) Moléculas de ácido nucleico de arn polimerasa ii33 para el control de plagas de insectos
CL2017001523A1 (es) Supresion parental rnai del gen hunchback para el control de plagas de coleopteros
CL2017003012A1 (es) Moléculas de ácidos nucleicos de spt5 para controlar plagas de insectos.
AR105257A1 (es) Moléculas de fusión que tiene especificidad de unión a pioverdina y pioquelina
EP3543331A4 (en) CELL POPULATION CONTAINING MESENCHYMAL STEM CELLS DERIVED FROM F RATE APPENDICES, METHOD FOR PRODUCING THE SAME, AND MEDICINAL COMPOSITION
AR094339A1 (es) Método y composiciones para el control genético de insectos plaga en plantas de algodón a través del silenciamiento de genes de la familia de la lacasa
AR087578A1 (es) Metodos para aumentar el contenido de proteinas, aceites y/o aminoacidos en una planta

Legal Events

Date Code Title Description
FC Refusal