AR082114A1 - Metodo para la preparacion de 1,3-propanodiol a partir de sacarosa - Google Patents
Metodo para la preparacion de 1,3-propanodiol a partir de sacarosaInfo
- Publication number
- AR082114A1 AR082114A1 ARP110102402A ARP110102402A AR082114A1 AR 082114 A1 AR082114 A1 AR 082114A1 AR P110102402 A ARP110102402 A AR P110102402A AR P110102402 A ARP110102402 A AR P110102402A AR 082114 A1 AR082114 A1 AR 082114A1
- Authority
- AR
- Argentina
- Prior art keywords
- propanediol
- sucrose
- microorganism
- source
- enzyme
- Prior art date
Links
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 title abstract 5
- 229930006000 Sucrose Natural products 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 2
- 238000002360 preparation method Methods 0.000 title abstract 2
- DNIAPMSPPWPWGF-VKHMYHEASA-N (+)-propylene glycol Chemical compound C[C@H](O)CO DNIAPMSPPWPWGF-VKHMYHEASA-N 0.000 abstract 4
- YPFDHNVEDLHUCE-UHFFFAOYSA-N 1,3-propanediol Substances OCCCO YPFDHNVEDLHUCE-UHFFFAOYSA-N 0.000 abstract 4
- 244000005700 microbiome Species 0.000 abstract 4
- 229920000166 polytrimethylene carbonate Polymers 0.000 abstract 4
- 239000005720 sucrose Substances 0.000 abstract 4
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 2
- 238000004519 manufacturing process Methods 0.000 abstract 2
- AKXKFZDCRYJKTF-UHFFFAOYSA-N 3-Hydroxypropionaldehyde Chemical compound OCCC=O AKXKFZDCRYJKTF-UHFFFAOYSA-N 0.000 abstract 1
- PUWWONYMIXRVQF-UHFFFAOYSA-N 4-Hydroxy-2-oxobutanoic acid Chemical compound OCCC(=O)C(O)=O PUWWONYMIXRVQF-UHFFFAOYSA-N 0.000 abstract 1
- 239000002028 Biomass Substances 0.000 abstract 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 1
- 102000004031 Carboxy-Lyases Human genes 0.000 abstract 1
- 108090000489 Carboxy-Lyases Proteins 0.000 abstract 1
- 241000588724 Escherichia coli Species 0.000 abstract 1
- 102000004316 Oxidoreductases Human genes 0.000 abstract 1
- 108090000854 Oxidoreductases Proteins 0.000 abstract 1
- 229910052799 carbon Inorganic materials 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 238000006114 decarboxylation reaction Methods 0.000 abstract 1
- 238000000855 fermentation Methods 0.000 abstract 1
- 230000004151 fermentation Effects 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000008725 two step metabolic pathway Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01021—Aldehyde reductase (1.1.1.21), i.e. aldose-reductase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y401/00—Carbon-carbon lyases (4.1)
- C12Y401/01—Carboxy-lyases (4.1.1)
- C12Y401/01001—Pyruvate decarboxylase (4.1.1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y206/00—Transferases transferring nitrogenous groups (2.6)
- C12Y206/01—Transaminases (2.6.1)
- C12Y206/01052—Phosphoserine transaminase (2.6.1.52)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Microorganismo modificado genéticamente para la producción biológica de 1,3-propanodiol a partir de sacarosa, en el cual el microorganismo comprende: una vía metabólica de dos pasos para la producción de 1,3-propanodiol, la cual comprende un primer paso de descarboxilación de 4-hidroxi-2-cetobutirato con una enzima que tiene una actividad descarboxilasa del ácido 2-ceto, y un segundo paso de reducción del 3-hidroxipropionaldehído obtenido con una enzima que tiene actividad hidroxialdehído reductasa, y genes que permiten que el microorganismo utilice sacarosa como única fuente de carbono. También se refiere a un método para la preparación biológica de 1,3-propanodiol por fermentación, el cual comprende cultivar dicho microorganismo modificado genéticamente, en el cual el cultivo se lleva a cabo en un medio adecuado, el cual comprende una fuente de sacarosa, y recuperar el 1,3-propanodiol que se produce. En un aspecto preferido, la fuente de sacarosa se obtiene de la biomasa de plantas. Los ejemplos muestran cepas modificadas de Escherichia coli.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US36145510P | 2010-07-05 | 2010-07-05 | |
| EP10305729 | 2010-07-05 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR082114A1 true AR082114A1 (es) | 2012-11-14 |
Family
ID=42712776
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP110102402A AR082114A1 (es) | 2010-07-05 | 2011-07-05 | Metodo para la preparacion de 1,3-propanodiol a partir de sacarosa |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US8900838B2 (es) |
| EP (1) | EP2591091B1 (es) |
| JP (1) | JP5420798B2 (es) |
| KR (1) | KR101794384B1 (es) |
| CN (1) | CN103097514B (es) |
| AR (1) | AR082114A1 (es) |
| BR (1) | BR112013000237B1 (es) |
| CA (1) | CA2803070C (es) |
| ES (1) | ES2490968T3 (es) |
| PT (1) | PT2591091E (es) |
| TW (1) | TWI500768B (es) |
| WO (1) | WO2012004247A1 (es) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2872640B1 (en) * | 2012-07-11 | 2020-03-25 | Adisseo France S.A.S. | Method for the preparation of 2,4-dihydroxybutyrate |
| EP2872639B1 (en) | 2012-07-11 | 2018-03-14 | Institut National Des Sciences Appliquees | A microorganism modified for the production of 1,3-propanediol |
| WO2016050959A2 (en) * | 2014-10-03 | 2016-04-07 | Metabolic Explorer | Mutant glutamate dehydrogenase for the conversion of homoserine into 4-hydroxy-2-ketobutyrate |
| EP3050970B1 (en) | 2015-01-28 | 2019-09-18 | Metabolic Explorer | Modified microorganism for optimized production of 1,4-butanediol |
| WO2016162712A1 (en) | 2015-04-07 | 2016-10-13 | Metabolic Explorer | Modified microorganism for the optimized production of 2,4-dihydroxyburyrate |
| KR102535038B1 (ko) | 2015-09-08 | 2023-05-23 | 에스케이플래닛 주식회사 | 모바일 커머스 서비스 장치, 초음파 송수신 장치를 이용한 모바일 커머스 서비스 방법 및 컴퓨터 프로그램이 기록된 기록매체 |
| EP3481850A1 (en) | 2016-07-08 | 2019-05-15 | Evonik Degussa GmbH | Method for the fermentative production of methionine or its hydroxy analog form by microorganisms comprising genes coding sugar phosphotransferase system (pts) |
| EP3470512A1 (en) | 2017-10-10 | 2019-04-17 | Metabolic Explorer | Mutant phosphoserine aminotransferase for the conversion of homoserine into 4-hydroxy-2-ketobutyrate |
| CN108384796B (zh) * | 2018-03-05 | 2021-11-26 | 北京化工大学 | 一种利用蔗糖生物合成d-葡萄糖二酸的方法 |
| JP6668577B1 (ja) * | 2018-10-30 | 2020-03-18 | ＧｒｅｅｎＥａｒｔｈＩｎｓｔｉｔｕｔｅ株式会社 | １，３−プロパンジオールの製造方法 |
| CN111705030B (zh) * | 2020-07-07 | 2023-03-31 | 浙江工业大学 | 高产l-高丝氨酸的大肠杆菌基因工程菌、构建方法及菌株 |
Family Cites Families (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5686276A (en) * | 1995-05-12 | 1997-11-11 | E. I. Du Pont De Nemours And Company | Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism |
| FI105753B (fi) * | 1997-12-31 | 2000-09-29 | Ssh Comm Security Oy | Pakettien autentisointimenetelmä verkko-osoitemuutosten ja protokollamuunnosten läsnäollessa |
| US6514733B1 (en) * | 1999-08-18 | 2003-02-04 | E. I. Du Pont De Nemours And Company | Process for the biological production of 1,3-propanediol with high titer |
| RU2212447C2 (ru) | 2000-04-26 | 2003-09-20 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" | Штамм escherichia coli - продуцент аминокислоты (варианты) и способ получения аминокислот (варианты) |
| CA2501574C (en) * | 2002-10-04 | 2013-12-03 | E. I. Du Pont De Nemours And Company | Process for the biological production of 1,3-propanediol with high yield |
| US20060270013A1 (en) | 2003-02-18 | 2006-11-30 | Michel Chateau | Method for the production of evolved microorganisms which permit the generation or modification of metabolic pathways |
| CA2522928C (en) | 2003-05-06 | 2013-09-24 | E.I. Du Pont De Nemours And Company | Purification of biologically-produced 1,3-propanediol |
| FR2862068B1 (fr) | 2003-11-06 | 2007-10-12 | Metabolic Explorer Sa | Souches de microorganismes optimisees pour des voies de biosyntheses consommatrices de nadph |
| FR2864967B1 (fr) | 2004-01-12 | 2006-05-19 | Metabolic Explorer Sa | Microorganisme evolue pour la production de 1,2-propanediol |
| WO2005111202A1 (en) | 2004-05-12 | 2005-11-24 | Metabolic Explorer | Recombinant enzyme with altered feedback sensitivity |
| MX2007009489A (es) | 2005-02-07 | 2007-09-19 | Metabolic Explorer Sa | Microorganismos que comprenden enzimas expresadas con baja actividad de eliminacion gamma. |
| EP1846564A2 (en) | 2005-02-07 | 2007-10-24 | Metabolic Explorer | Method for the enzymatic production of alpha-ketobutyrate |
| WO2007017710A1 (en) | 2005-08-11 | 2007-02-15 | Metabolic Explorer | Process for the preparation of aspartate and derived amino acids like lysine, threonine, isoleucine, methionine, homoserine, or valine employing a microorganism with enhanced isocitrate lyase and/or malate synthase expression |
| ES2459617T3 (es) | 2006-01-04 | 2014-05-12 | Metabolic Explorer | Procedimiento para la preparación de metionina y sus precursores homoserina o succinilhomoserina que utiliza un microorganismo |
| DE102006003535A1 (de) | 2006-01-24 | 2007-08-02 | Schott Ag | Verfahren zur Temperaturbeeinflussung einer Schmelze |
| EP1991688B1 (en) | 2006-02-10 | 2019-05-01 | DuPont Tate & Lyle Bio Products Company, LLC | Biodegradable compositions comprising renewably-based, biodegradable 1.3-propanediol |
| WO2007140816A1 (en) | 2006-06-09 | 2007-12-13 | Metabolic Explorer | Glycolic acid production by fermentation from renewable resources |
| WO2007144018A1 (en) | 2006-06-12 | 2007-12-21 | Metabolic Explorer | Ethanolamine production by fermentation |
| AU2006349179A1 (en) | 2006-10-03 | 2008-04-10 | Metabolic Explorer | Process for chromosomal integration and DNA sequence replacement in Clostridia |
| WO2008052596A1 (en) | 2006-10-31 | 2008-05-08 | Metabolic Explorer | Process for the biological production of n-butanol with high yield |
| KR20090090319A (ko) | 2006-10-31 | 2009-08-25 | 메타볼릭 익스플로러 | 높은 수율로 글리세롤로부터 1,3-프로판디올을 생물학적으로 생산하는 방법 |
| CN101688175A (zh) * | 2007-02-09 | 2010-03-31 | 加利福尼亚大学董事会 | 利用重组微生物生产生物燃料 |
| KR101543485B1 (ko) | 2007-11-30 | 2015-08-10 | 메타볼릭 익스플로러 | 발효 브로쓰로부터 알콜을 정제하는 방법 |
| CA2738452C (en) | 2008-10-03 | 2016-06-21 | Metabolic Explorer | Method for purifying an alcohol from a fermentation broth using a falling film, a wiped film, a thin film or a short path evaporator |
| US20110217744A1 (en) * | 2008-11-07 | 2011-09-08 | Metabolic Explorer | Use of sucrose as substrate for fermentative production of 1,2-propanediol |
| US8192976B2 (en) * | 2008-12-12 | 2012-06-05 | Celexion, Llc | Biological synthesis of difunctional alkanes from carbohydrate feedstocks |
| KR20110117131A (ko) * | 2008-12-31 | 2011-10-26 | 메타볼릭 익스플로러 | 디올의 제조 방법 |
| EP2248904A1 (en) | 2009-05-05 | 2010-11-10 | Metabolic Explorer | Continuous culture for 1,3-propanediol production using high glycerine concentration |
-
2011
- 2011-07-04 TW TW100123460A patent/TWI500768B/zh active
- 2011-07-05 EP EP11728895.1A patent/EP2591091B1/en active Active
- 2011-07-05 US US13/808,251 patent/US8900838B2/en active Active
- 2011-07-05 CN CN201180042798.0A patent/CN103097514B/zh active Active
- 2011-07-05 PT PT117288951T patent/PT2591091E/pt unknown
- 2011-07-05 KR KR1020137002898A patent/KR101794384B1/ko active Active
- 2011-07-05 BR BR112013000237-9A patent/BR112013000237B1/pt active IP Right Grant
- 2011-07-05 WO PCT/EP2011/061285 patent/WO2012004247A1/en not_active Ceased
- 2011-07-05 JP JP2013517354A patent/JP5420798B2/ja not_active Expired - Fee Related
- 2011-07-05 CA CA2803070A patent/CA2803070C/en active Active
- 2011-07-05 AR ARP110102402A patent/AR082114A1/es unknown
- 2011-07-05 ES ES11728895.1T patent/ES2490968T3/es active Active
Also Published As
| Publication number | Publication date |
|---|---|
| JP2013529924A (ja) | 2013-07-25 |
| CN103097514A (zh) | 2013-05-08 |
| US20130309737A1 (en) | 2013-11-21 |
| TW201213545A (en) | 2012-04-01 |
| ES2490968T3 (es) | 2014-09-04 |
| CA2803070A1 (en) | 2012-01-12 |
| EP2591091A1 (en) | 2013-05-15 |
| CA2803070C (en) | 2019-10-08 |
| BR112013000237B1 (pt) | 2021-05-04 |
| PT2591091E (pt) | 2014-08-29 |
| CN103097514B (zh) | 2015-09-09 |
| KR101794384B1 (ko) | 2017-11-06 |
| KR20130041166A (ko) | 2013-04-24 |
| JP5420798B2 (ja) | 2014-02-19 |
| BR112013000237A2 (pt) | 2016-05-17 |
| WO2012004247A1 (en) | 2012-01-12 |
| TWI500768B (zh) | 2015-09-21 |
| US8900838B2 (en) | 2014-12-02 |
| EP2591091B1 (en) | 2014-05-14 |
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Legal Events
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| FB | Suspension of granting procedure |