AR086210A2 - Promotores disimiles para supresion genica - Google Patents
Promotores disimiles para supresion genicaInfo
- Publication number
- AR086210A2 AR086210A2 ARP120101510A ARP120101510A AR086210A2 AR 086210 A2 AR086210 A2 AR 086210A2 AR P120101510 A ARP120101510 A AR P120101510A AR P120101510 A ARP120101510 A AR P120101510A AR 086210 A2 AR086210 A2 AR 086210A2
- Authority
- AR
- Argentina
- Prior art keywords
- promoter
- gene
- corn plants
- recombinant dna
- plant
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 14
- 230000001629 suppression Effects 0.000 title abstract 5
- 241001057636 Dracaena deremensis Species 0.000 abstract 13
- 241000196324 Embryophyta Species 0.000 abstract 7
- 108020004511 Recombinant DNA Proteins 0.000 abstract 7
- 235000015097 nutrients Nutrition 0.000 abstract 6
- 230000009261 transgenic effect Effects 0.000 abstract 5
- 240000008042 Zea mays Species 0.000 abstract 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 abstract 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract 4
- 235000005822 corn Nutrition 0.000 abstract 4
- 210000004027 cell Anatomy 0.000 abstract 3
- 150000001413 amino acids Chemical class 0.000 abstract 2
- 150000001720 carbohydrates Chemical class 0.000 abstract 2
- 230000015556 catabolic process Effects 0.000 abstract 2
- 238000010276 construction Methods 0.000 abstract 2
- 230000030279 gene silencing Effects 0.000 abstract 2
- 150000002632 lipids Chemical class 0.000 abstract 2
- 210000001161 mammalian embryo Anatomy 0.000 abstract 2
- 238000013518 transcription Methods 0.000 abstract 2
- 230000035897 transcription Effects 0.000 abstract 2
- 210000000349 chromosome Anatomy 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 210000004940 nucleus Anatomy 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/823—Reproductive tissue-specific promoters
- C12N15/8234—Seed-specific, e.g. embryo, endosperm
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Pregnancy & Childbirth (AREA)
- Developmental Biology & Embryology (AREA)
- Reproductive Health (AREA)
- Virology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Reivindicación 1: Un método para manufacturar semilla de maíz transgénica con un aumento del nivel de un nutriente, caracterizado porque comprende: (a) seleccionar una primera planta de maíz transgénica que comprende la construcción de DNA recombinante que comprende: un primer promotor ligado operativamente a por lo menos un primer elemento de supresión génica para silenciar al menos un primer gen objetivo, y un segundo promotor que está en la orientación opuesta a dicho primer promotor y está situado 3’ a dicho al menos un primer elemento de supresión génica, donde dichos primer y segundo promotores comprenden un promotor vegetal específico de embrión y un promotor vegetal específico de endosperma y donde la transcripción de dicha construcción de ADN recombinante en una célula vegetal da como resultado el silenciamiento de dicho al menos un primer gen objetivo, caracterizada porque dicho por lo menos un primer gen objetivo es un gen del catabolismo de un nutriente seleccionado entre un aminoácido, un lípido o un carbohidrato; (b) efectuar la introgresión de dicha construcción de DNA recombinante en una segunda planta de maíz; (c) cultivar una semilla de la segunda planta de maíz para producir una población de plantas de maíz de progenie; (d) evaluar la población de plantas de maíz de progenie para seleccionar las plantas de maíz de progenie que producen semilla de maíz con un nivel aumentado de dicho nutriente, con relación a las plantas de maíz no transgénicas; (e) seleccionar de dicha población una o más plantas de maíz de progenie que producen semilla de maíz con un aumento del nivel de dicho nutriente, con relación a las plantas de maíz no transgénicas; (f) verificar que dicha construcción de DNA recombinante esté integrada de manera estable en dichas plantas de maíz de progenie seleccionadas; (g) verificar que dicho gen del catabolismo de dicho nutriente esté silenciado en dichas plantas de maíz de progenie seleccionadas, con relación a las plantas de maíz que carecen de dicha construcción de DNA recombinante; (h) recolectar una semilla de maíz transgénica de dichas plantas de maíz de progenie seleccionadas.Reivindicación 3: Un núcleo de una célula de una planta, caracterizado porque dicho núcleo tiene por lo menos un cromosoma que comprende la construcción de DNA recombinante que comprende: un primer promotor ligado operativamente a por lo menos un primer elemento de supresión génica para silenciar al menos un primer gen objetivo, y un segundo promotor que está en la orientación opuesta a dicho primer promotor y está situado 3’ a dicho al menos un primer elemento de supresión génica, donde dichos primer y segundo promotores comprenden un promotor vegetal específico de embrión y un promotor vegetal específico de endosperma y donde la transcripción de dicha construcción de ADN recombinante en una célula vegetal da como resultado el silenciamiento de dicho al menos un primer gen objetivo, caracterizada porque dicho por lo menos un primer gen objetivo es un gen del catabolismo de un nutriente seleccionado entre un aminoácido, un lípido o un carbohidrato.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11/311,892 US20060150286A1 (en) | 2004-12-23 | 2005-12-19 | Gene suppression in transgenic plants using multiple constructs |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR086210A2 true AR086210A2 (es) | 2013-11-27 |
Family
ID=38000795
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP060101695A AR053076A1 (es) | 2005-12-19 | 2006-04-27 | Promotores disimiles para supresion genica |
| ARP120101510A AR086210A2 (es) | 2005-12-19 | 2012-04-27 | Promotores disimiles para supresion genica |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP060101695A AR053076A1 (es) | 2005-12-19 | 2006-04-27 | Promotores disimiles para supresion genica |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20060150286A1 (es) |
| EP (1) | EP1963488A4 (es) |
| CN (2) | CN101374943A (es) |
| AR (2) | AR053076A1 (es) |
| AU (1) | AU2006327232A1 (es) |
| WO (1) | WO2007073394A1 (es) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070074311A1 (en) | 2005-08-30 | 2007-03-29 | Pioneer Hi-Bred International, Inc. | Compositions and methods for modulating expression of gene products |
| WO2007059092A1 (en) * | 2005-11-14 | 2007-05-24 | Pioneer Hi-Bred International, Inc. | Methods and compositions for reducing the expression of a polynucleotide of interest |
| CN109642235B (zh) * | 2016-06-28 | 2023-12-29 | 孟山都技术公司 | 用于植物中的基因组修饰的方法和组合物 |
| CN110288076B (zh) * | 2019-07-09 | 2020-03-27 | 中央民族大学 | 指示信号在不同伴随信号下优先级的细菌细胞计算部件 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5258300A (en) * | 1988-06-09 | 1993-11-02 | Molecular Genetics Research And Development Limited Partnership | Method of inducing lysine overproduction in plants |
| US5773691A (en) * | 1992-03-19 | 1998-06-30 | E. I. Du Pont De Nemours And Company | Chimeric genes and methods for increasing the lysine and threonine content of the seeds of plants |
| US20050005330A1 (en) * | 1994-01-06 | 2005-01-06 | Falco Saverio Carl | Chimeric genes and methods for increasing the lysine content of the seeds of plants |
| AR020078A1 (es) * | 1998-05-26 | 2002-04-10 | Syngenta Participations Ag | Metodo para alterar la expresion de un gen objetivo en una celula de planta |
| US6849779B1 (en) * | 1998-08-27 | 2005-02-01 | Rutgers, The State University Of New Jersey | Method for producing high methionine corn seeds |
| US6326193B1 (en) * | 1999-11-05 | 2001-12-04 | Cambria Biosciences, Llc | Insect control agent |
| US6972349B1 (en) * | 1999-11-12 | 2005-12-06 | University Of South Carolina | Control of post-transcriptional gene silencing in plants |
| US20020182223A1 (en) * | 2000-06-02 | 2002-12-05 | Lacount Douglas J. | Method of rapidly generating double-stranded RNA and methods of use thereof |
| EP1373527A1 (en) * | 2001-04-06 | 2004-01-02 | CropDesign N.V. | The use of double and opposite recombination sites for the single step cloning of two dna segments |
| WO2005040388A2 (en) * | 2003-08-22 | 2005-05-06 | Nucleonics Inc. | Eukariotic expression systems for expression of inhibitory rna in multiple intracellular compartments |
| WO2005026322A2 (en) * | 2003-09-11 | 2005-03-24 | Clontech Laboratories, Inc. | siRNA ENCODING CONSTRUCTS AND METHODS FOR USING THE SAME |
| US7855323B2 (en) * | 2004-02-10 | 2010-12-21 | Monsanto Technology Llc | Recombinant DNA for gene suppression |
| AR047598A1 (es) * | 2004-02-10 | 2006-01-25 | Monsanto Technology Llc | Semilla de maiz transgenica con mayor contenido de aminoacidos |
| WO2005121347A2 (en) * | 2004-06-09 | 2005-12-22 | E.I. Dupont De Nemours And Company | Recombinant constructs for use in reducing gene expression |
| DK1799028T3 (en) * | 2004-09-24 | 2015-03-23 | Simplot Co J R | Gene silencing |
| US20070074311A1 (en) * | 2005-08-30 | 2007-03-29 | Pioneer Hi-Bred International, Inc. | Compositions and methods for modulating expression of gene products |
| WO2007041419A1 (en) * | 2005-10-03 | 2007-04-12 | Monsanto Technology Llc | Transgenic plant seed with increased lysine |
-
2005
- 2005-12-19 US US11/311,892 patent/US20060150286A1/en not_active Abandoned
-
2006
- 2006-03-31 CN CNA200680052963XA patent/CN101374943A/zh active Pending
- 2006-03-31 AU AU2006327232A patent/AU2006327232A1/en not_active Abandoned
- 2006-03-31 EP EP06740214A patent/EP1963488A4/en not_active Withdrawn
- 2006-03-31 WO PCT/US2006/011946 patent/WO2007073394A1/en not_active Ceased
- 2006-04-27 AR ARP060101695A patent/AR053076A1/es not_active Application Discontinuation
- 2006-10-23 CN CN200680047649.2A patent/CN101389212B/zh not_active Expired - Fee Related
-
2012
- 2012-04-27 AR ARP120101510A patent/AR086210A2/es not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| CN101389212A (zh) | 2009-03-18 |
| CN101389212B (zh) | 2013-05-01 |
| EP1963488A1 (en) | 2008-09-03 |
| AR053076A1 (es) | 2007-04-18 |
| US20060150286A1 (en) | 2006-07-06 |
| EP1963488A4 (en) | 2009-02-04 |
| AU2006327232A1 (en) | 2007-06-28 |
| CN101374943A (zh) | 2009-02-25 |
| WO2007073394A1 (en) | 2007-06-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA | Abandonment or withdrawal |