AR046991A1 - Metodo para la preparacion de un soporte solido listo para usar para ensayo inmunosorbente ligado a enzima (elisa) rapido - Google Patents
Metodo para la preparacion de un soporte solido listo para usar para ensayo inmunosorbente ligado a enzima (elisa) rapidoInfo
- Publication number
- AR046991A1 AR046991A1 ARP040104875A ARP040104875A AR046991A1 AR 046991 A1 AR046991 A1 AR 046991A1 AR P040104875 A ARP040104875 A AR P040104875A AR P040104875 A ARP040104875 A AR P040104875A AR 046991 A1 AR046991 A1 AR 046991A1
- Authority
- AR
- Argentina
- Prior art keywords
- solid support
- ready
- wells
- elisa
- degree
- Prior art date
Links
- 239000007787 solid Substances 0.000 title abstract 11
- 238000000034 method Methods 0.000 title abstract 5
- 238000002965 ELISA Methods 0.000 title abstract 3
- 102000004190 Enzymes Human genes 0.000 title abstract 2
- 108090000790 Enzymes Proteins 0.000 title abstract 2
- 238000002360 preparation method Methods 0.000 title abstract 2
- 239000000872 buffer Substances 0.000 abstract 3
- 239000000427 antigen Substances 0.000 abstract 2
- 102000036639 antigens Human genes 0.000 abstract 2
- 108091007433 antigens Proteins 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 239000000243 solution Substances 0.000 abstract 2
- 230000000087 stabilizing effect Effects 0.000 abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
- 239000011534 wash buffer Substances 0.000 abstract 2
- 238000008157 ELISA kit Methods 0.000 abstract 1
- 239000002981 blocking agent Substances 0.000 abstract 1
- 239000011248 coating agent Substances 0.000 abstract 1
- 238000000576 coating method Methods 0.000 abstract 1
- 239000013642 negative control Substances 0.000 abstract 1
- 239000013641 positive control Substances 0.000 abstract 1
- 239000011550 stock solution Substances 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Se provee un método para la preparacion de un soporte solido listo para usar con el método ELISA para la rápida identificacion y estimacion cuantitativa de una proteína/antígeno en las muestras de prueba y el rápido rendimiento del ensayo mismo. Además se provee una estimacion rápida, precisa y estable de una proteína/antígeno en las muestras de prueba. Además, también proporciona un kit ELISA que comprende un soporte solido listo para usar junto con buferes de lavado, un sustrato químico, un bufer de sustrato, la solucion madre, y muestras de control positivas y negativas. Reivindicacion 1: Un método para preparar un soporte solido lista para usar en el método ELISA rápido, caracterizado porque comprende los siguientes pasos; a) agregar un primer anticuerpo monoclonal disuelto en un bufer de recubrimiento a los pocillos del soporte solido, incubar el soporte solido a alrededor de 4 degree C durante un período comprendido entre alrededor de 12 y 14 horas para lograr la union con el soporte solido; b) lavar el soporte solido del paso a), con un bufer de lavado para remover el anticuerpo monoclonal no ligado; c) agregar una solucion estabilizadora a los pocillos del soporte solido del paso b), incubar durante un período comprendido entre 12 y 14 horas a alrededor de 4 degree C; d) hacer decantar para remover la solucion estabilizadora del paso c), y secar completamente los pocillos del soporte solido; e) agregar a los pocillos del soporte solido del paso d), un segundo anticuerpo adecuado y un tercer anticuerpo adecuado conjugado con una enzima disuelta en un bufer adecuado que contenga el agente bloqueante; y f) liofilizar la placa del paso e), almacenar la placa en un envase sellado a una temperatura comprendida entre alrededor de 4-8 degree C para dejarla lista para usar.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IN1600DE2003 | 2003-12-23 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR046991A1 true AR046991A1 (es) | 2006-01-04 |
Family
ID=34708482
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP040104875A AR046991A1 (es) | 2003-12-23 | 2004-12-22 | Metodo para la preparacion de un soporte solido listo para usar para ensayo inmunosorbente ligado a enzima (elisa) rapido |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20080044928A1 (es) |
| AR (1) | AR046991A1 (es) |
| AU (1) | AU2004304105B2 (es) |
| BR (1) | BRPI0418084A (es) |
| WO (1) | WO2005062050A1 (es) |
| ZA (1) | ZA200605547B (es) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103235147B (zh) | 2005-12-21 | 2015-08-12 | 梅索斯卡莱科技公司 | 分析装置的液体分配器 |
| EP3431994B1 (en) | 2005-12-21 | 2024-03-06 | Meso Scale Technologies, LLC. | Assay modules having assay reagents and methods of making and using same |
| GB0711683D0 (en) * | 2007-06-16 | 2007-07-25 | Enigma Diagnostics Ltd | Compositions |
| EP2236520A1 (en) * | 2009-03-31 | 2010-10-06 | Leukocare Ag | Stabilizing composition for immobilized biomolecules |
| CN102288769A (zh) * | 2011-05-10 | 2011-12-21 | 中国检验检疫科学研究院 | 一种检测Bt cry1 Ac蛋白的液相芯片及其应用 |
| CN102590527B (zh) * | 2012-01-16 | 2014-04-23 | 中国农业大学 | 检测杀虫晶体蛋白Bt Cry1Ab/Ac的方法及其专用酶联免疫试剂盒 |
| CN106674334B (zh) * | 2017-02-08 | 2020-09-01 | 金陵科技学院 | 一种结合Cry2Ad的环七肽及其编码基因和应用 |
| CN108254560A (zh) * | 2018-04-19 | 2018-07-06 | 国家食品安全风险评估中心 | 牛奶中黄曲霉毒素m1高通量酶联免疫试剂盒及其检测方法 |
| US20220276235A1 (en) * | 2019-07-18 | 2022-09-01 | Essenlix Corporation | Imaging based homogeneous assay |
| CN113433316B (zh) * | 2020-03-23 | 2025-07-25 | 常州健益生物制药有限公司 | 一种用于检测氨肽酶含量的试剂盒、及氨肽酶含量的检测方法 |
| CN116087503A (zh) * | 2023-01-08 | 2023-05-09 | 杭州联科生物技术股份有限公司 | 一种实现elisa简易操作的方法 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63111467A (ja) * | 1986-10-30 | 1988-05-16 | Tosoh Corp | 免疫測定方法 |
| JPH02161357A (ja) * | 1988-12-15 | 1990-06-21 | Tosoh Corp | 免疫測定用材料の安定化方法 |
| JPH0815261A (ja) * | 1994-06-24 | 1996-01-19 | Nkk Corp | 免疫測定用材料の製造方法 |
| US5602041A (en) * | 1994-08-26 | 1997-02-11 | Sea Run Holdings, Inc. | Fish serum as a blocking reagent |
| US6017534A (en) * | 1996-11-20 | 2000-01-25 | Ecogen, Inc. | Hybrid Bacillus thuringiensis δ-endotoxins with novel broad-spectrum insecticidal activity |
| US6489542B1 (en) * | 1998-11-04 | 2002-12-03 | Monsanto Technology Llc | Methods for transforming plants to express Cry2Ab δ-endotoxins targeted to the plastids |
| US20040254364A1 (en) * | 1999-07-30 | 2004-12-16 | Adang Michael J. | Phage display of a biologically active Bacillus thuringiensis toxin |
| US6586197B1 (en) * | 1999-09-07 | 2003-07-01 | University Of Georgia Research Foundation, Inc. | Methods and materials for identifying novel pesticide agents |
| US7141436B2 (en) * | 1999-11-03 | 2006-11-28 | Science And Technology Corp. | Immunoassay and reagents and kits for performing the same |
| DK1309864T3 (da) * | 2000-08-16 | 2006-04-18 | Council Scient Ind Res | Hurtig fremgangsmåde til mikrobölgemedieret enzymbundet immunosorbentassay |
| DK1344059T3 (da) * | 2000-12-22 | 2008-02-11 | Bio A R T Sa | Gennemströmningsassayindretning, diagnostisk kit som omfatter indretningen og anvendelse af assayindretningen til påvisning af en analyt i en pröve |
| WO2002079781A1 (en) * | 2001-03-28 | 2002-10-10 | Heska Corporation | Methods of detecting early renal disease in animals |
| AT5044U1 (de) * | 2001-05-10 | 2002-02-25 | Medsystems Diagnostics Gmbh | Quantitativer einschritt immuntest in lyophilisierter form |
| ITMI20012160A1 (it) * | 2001-10-18 | 2003-04-18 | Edoardo Marchisio | Sistema immunoenzimatico per la caratterizzazione antigenica dell'infezione attiva da cytomegalovirus e cmv confirmation test |
-
2004
- 2004-12-22 AR ARP040104875A patent/AR046991A1/es active IP Right Grant
- 2004-12-22 US US10/583,751 patent/US20080044928A1/en not_active Abandoned
- 2004-12-22 WO PCT/IN2004/000394 patent/WO2005062050A1/en not_active Ceased
- 2004-12-22 BR BRPI0418084-4A patent/BRPI0418084A/pt not_active IP Right Cessation
- 2004-12-22 AU AU2004304105A patent/AU2004304105B2/en not_active Ceased
-
2006
- 2006-07-05 ZA ZA200605547A patent/ZA200605547B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| BRPI0418084A (pt) | 2007-04-17 |
| WO2005062050A1 (en) | 2005-07-07 |
| AU2004304105B2 (en) | 2011-05-12 |
| US20080044928A1 (en) | 2008-02-21 |
| AU2004304105A1 (en) | 2005-07-07 |
| WO2005062050B1 (en) | 2005-09-09 |
| ZA200605547B (en) | 2007-11-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant, registration |