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AR039939A1 - MODIFIED PHYTASES - Google Patents

MODIFIED PHYTASES

Info

Publication number
AR039939A1
AR039939A1 ARP030101937A ARP030101937A AR039939A1 AR 039939 A1 AR039939 A1 AR 039939A1 AR P030101937 A ARP030101937 A AR P030101937A AR P030101937 A ARP030101937 A AR P030101937A AR 039939 A1 AR039939 A1 AR 039939A1
Authority
AR
Argentina
Prior art keywords
phytase
modified
model
phytases
compared
Prior art date
Application number
ARP030101937A
Other languages
Spanish (es)
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Publication of AR039939A1 publication Critical patent/AR039939A1/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fodder In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente describe fitasas modificadas. Estas fitasas están modificadas en comparación con una fitasa modelo en diversas posiciones, en particular para incrementar la termoestabilidad de la fitasa modificada en comparación con la de la fitasa modelo. Las fitasas modificadas han retenido otras propiedades favorables de la fitasa de Aspergillus niger, en particular porque en la fitasa modificada se han retenido residuos de aminoácidos específicos de la fitasa de Aspergillus niger. Reivindicación 1: Un polipéptido, caracterizado porque cuando se alinea con una fitasa ,modelo está modificado en comparación con dicha fitasa modelo en al menos una de las siguientes posiciones: 5, 6, 13, 19, 21, 29, 31, 36, 39, 43, 53, 69, 78, 81, 85, 87, 99, 112, 113, 122, 125, 126, 128,137,147, 148, 157,160, 163, 165, 169, 172, 176, 178, 180, 181, 182, 183, 189, 194, 197, 201, 203, 211, 213, 215, 218, 222, 223, 225, 232, 233, 242, 246, 247, 248, 249, 250, 251, 252, 254, 269, 291, 296, 310, 312, 315, 322, 330, 342, 346, 362, 365, 367, 368, 372, 374, 375, 382, 384, 395, 414, 417, 425, 428, 438, 440.This describes modified phytases. These phytases are modified compared to a phytase model in various positions, in particular to increase the thermostability of the modified phytase compared to that of the phytase model. Modified phytases have retained other favorable properties of Aspergillus niger phytase, in particular because specific amino acid residues of Aspergillus niger phytase have been retained in the modified phytase. Claim 1: A polypeptide, characterized in that when aligned with a phytase, model is modified compared to said model phytase in at least one of the following positions: 5, 6, 13, 19, 21, 29, 31, 36, 39 , 43, 53, 69, 78, 81, 85, 87, 99, 112, 113, 122, 125, 126, 128,137,147, 148, 157,160, 163, 165, 169, 172, 176, 178, 180, 181, 182 , 183, 189, 194, 197, 201, 203, 211, 213, 215, 218, 222, 223, 225, 232, 233, 242, 246, 247, 248, 249, 250, 251, 252, 254, 269 , 291, 296, 310, 312, 315, 322, 330, 342, 346, 362, 365, 367, 368, 372, 374, 375, 382, 384, 395, 414, 417, 425, 428, 438, 440 .

ARP030101937A 2002-05-30 2003-05-30 MODIFIED PHYTASES AR039939A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP02100608 2002-05-30

Publications (1)

Publication Number Publication Date
AR039939A1 true AR039939A1 (en) 2005-03-09

Family

ID=29595046

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP030101936A AR039938A1 (en) 2002-05-30 2003-05-30 MODIFIED PHYTASES
ARP030101937A AR039939A1 (en) 2002-05-30 2003-05-30 MODIFIED PHYTASES

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ARP030101936A AR039938A1 (en) 2002-05-30 2003-05-30 MODIFIED PHYTASES

Country Status (16)

Country Link
EP (1) EP1513931A2 (en)
JP (1) JP2005533492A (en)
KR (1) KR20050038593A (en)
CN (1) CN1656217A (en)
AR (2) AR039938A1 (en)
AU (1) AU2003238448A1 (en)
BR (1) BR0311287A (en)
CA (1) CA2485513A1 (en)
MX (1) MXPA04011224A (en)
NO (1) NO20044802L (en)
PE (1) PE20040537A1 (en)
PL (1) PL372824A1 (en)
RU (1) RU2329301C2 (en)
TW (2) TW200401033A (en)
WO (1) WO2003102174A2 (en)
ZA (1) ZA200410369B (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0422052D0 (en) * 2004-10-04 2004-11-03 Dansico As Enzymes
WO2007112739A1 (en) 2006-04-04 2007-10-11 Novozymes A/S Phytase variants
DE102006053059A1 (en) * 2006-11-10 2008-05-15 Ab Enzymes Gmbh Polypeptide with phytase activity and increased temperature stability of the enzyme activity and this coding nucleotide sequence
CN102854158B (en) * 2011-07-01 2016-01-20 北京昕大洋科技发展有限公司 A kind of method of Fast Measurement phytase heat resistance
CN105441406A (en) * 2014-08-05 2016-03-30 北京大学 Phytase variant
CN108251439B (en) * 2018-01-11 2021-03-30 山西大学 A kind of artificially modified trypsin-resistant phytase and its preparation method and application
CN117683745A (en) * 2020-04-29 2024-03-12 南京百斯杰生物工程有限公司 Parent phytase variant

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2316082A (en) * 1996-08-13 1998-02-18 Finnfeeds Int Ltd Phytase
EP1151088B1 (en) * 1999-01-22 2006-08-30 Novozymes A/S Improved phytases

Also Published As

Publication number Publication date
EP1513931A2 (en) 2005-03-16
BR0311287A (en) 2005-03-29
WO2003102174A2 (en) 2003-12-11
MXPA04011224A (en) 2005-01-25
KR20050038593A (en) 2005-04-27
JP2005533492A (en) 2005-11-10
RU2329301C2 (en) 2008-07-20
ZA200410369B (en) 2006-06-28
CN1656217A (en) 2005-08-17
PL372824A1 (en) 2005-08-08
TW200401033A (en) 2004-01-16
CA2485513A1 (en) 2003-12-11
NO20044802L (en) 2004-12-08
RU2004139054A (en) 2005-06-27
AU2003238448A1 (en) 2003-12-19
TW200403341A (en) 2004-03-01
WO2003102174A3 (en) 2004-03-25
AR039938A1 (en) 2005-03-09
PE20040537A1 (en) 2004-11-04

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Legal Events

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