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AR037988A1 - Fitasa tolerante a las variaciones de temperatura para alimento animal - Google Patents

Fitasa tolerante a las variaciones de temperatura para alimento animal

Info

Publication number
AR037988A1
AR037988A1 ARP020105150A ARP020105150A AR037988A1 AR 037988 A1 AR037988 A1 AR 037988A1 AR P020105150 A ARP020105150 A AR P020105150A AR P020105150 A ARP020105150 A AR P020105150A AR 037988 A1 AR037988 A1 AR 037988A1
Authority
AR
Argentina
Prior art keywords
tolerant
phytase
temperature variations
food
fitasa
Prior art date
Application number
ARP020105150A
Other languages
English (en)
Original Assignee
Syngenta Participations Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Participations Ag filed Critical Syngenta Participations Ag
Publication of AR037988A1 publication Critical patent/AR037988A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/20—Shaping or working-up of animal feeding-stuffs by moulding, e.g. making cakes or briquettes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/25—Shaping or working-up of animal feeding-stuffs by extrusion
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/30—Removing undesirable substances, e.g. bitter substances
    • A23L11/33—Removing undesirable substances, e.g. bitter substances using enzymes; Enzymatic transformation of pulses or legumes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/25—Removal of unwanted matter, e.g. deodorisation or detoxification using enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/03008—3-Phytase (3.1.3.8)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Polymers & Plastics (AREA)
  • Organic Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Animal Husbandry (AREA)
  • General Health & Medical Sciences (AREA)
  • Birds (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Physiology (AREA)
  • Agronomy & Crop Science (AREA)
  • Botany (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fodder In General (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

La presente proporciona un polinucleótido de fitasa sintético que es optimizado para la expresión en plantas, y que codifica a fitasa tolerante a las variaciones de temperatura, como así también enzima fitasa tolerante a las variaciones de temperatura aislada. Además se proporcionan alimentación o productos alimenticios que comprenden una fitasa tolerante a las variaciones de temperatura, y plantas transgénicas que expresan a la fitasa tolerante a las variaciones de temperatura. Se proporciona también métodos para la elaboración y uso de las fitasas tolerantes a las variaciones de temperatura, por ejemplo, un método de uso de un fitasa tolerante a las variaciones de temperatura en el procesamiento de alimentaciones o de alimentos. Reivindicación 1: Un método para la preparación de una fitasa tolerante a las variaciones de temperatura, que comprende: expresar en una célula de planta un cassette de expresión que comprende un promotor funcionalmente ligado a una molécula de ácido nucleico que codifica una fitasa tolerante a las variaciones de temperatura que retiene por lo menos 40% de actividad después de 30 minutos a 60°C y que tiene una actividad específica superior a 200 U/mg a pH 4,5 y a 37°C.
ARP020105150A 2001-12-28 2002-12-30 Fitasa tolerante a las variaciones de temperatura para alimento animal AR037988A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US34447601P 2001-12-28 2001-12-28

Publications (1)

Publication Number Publication Date
AR037988A1 true AR037988A1 (es) 2004-12-22

Family

ID=23350689

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP020105150A AR037988A1 (es) 2001-12-28 2002-12-30 Fitasa tolerante a las variaciones de temperatura para alimento animal

Country Status (11)

Country Link
US (4) US7141717B2 (es)
EP (1) EP1474165A4 (es)
CN (1) CN1622825A (es)
AR (1) AR037988A1 (es)
AU (1) AU2002364919A1 (es)
BR (1) BR0215406A (es)
CA (1) CA2471859A1 (es)
MX (1) MXPA04006389A (es)
TW (1) TW200305430A (es)
WO (1) WO2003057248A1 (es)
ZA (1) ZA200405088B (es)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MY139056A (en) * 2001-12-28 2009-08-28 Ab Enzymes Gmbh Microbially-expressed thermotolerant phytase for animal feed
US8114443B2 (en) * 2002-03-13 2012-02-14 Academia Sinica Phytase-expressing transgenic plants
BRPI0412372A (pt) * 2003-07-07 2006-09-05 Syngenta Participations Ag reagentes, métodos e kits para detectar enzimas da alimentação
CN1950510B (zh) 2004-03-08 2011-10-05 先正达合作有限公司 富含谷氨酰胺的玉米种子蛋白质和启动子
AR050895A1 (es) * 2004-10-04 2006-11-29 Novozymes As Polipeptidos que tienen actividad de fitasa y polinucleotidos que los codifican
WO2006037327A2 (en) * 2004-10-04 2006-04-13 Novozymes A/S Polypeptides having phytase activity and polynucleotides encoding same
US7658922B2 (en) 2005-06-24 2010-02-09 Ab Enzymes Gmbh Monoclonal antibodies, hybridoma cell lines, methods and kits for detecting phytase
GB0600913D0 (en) 2006-01-17 2006-02-22 Syngenta Ltd Improvements in or relating to organic compounds
DE102006053059A1 (de) 2006-11-10 2008-05-15 Ab Enzymes Gmbh Polypeptid mit Phytaseaktivität und erhöhter Temperaturstabilität der Enzymaktivität sowie dieses codierende Nukleotidsequenz
WO2008134068A2 (en) * 2007-04-26 2008-11-06 Hawaii Biotech, Inc. Synthetic expression vectors for insect cells
DK2283124T3 (en) * 2008-04-18 2016-09-05 Danisco Us Inc BUTTIAUXELLA SP. PHYTASE VARIANTS
HUE030948T2 (en) * 2009-05-21 2017-06-28 Basf Enzymes Llc Phytases, nucleic acids and processes encoding them for their production and use
ES2758563T3 (es) 2011-06-09 2020-05-05 Novozymes As Fusión de moléculas bioactivas
GB201213801D0 (en) * 2012-08-03 2012-09-12 Dupont Nutrition Biosci Aps Feed additive composition
CN102864167B (zh) * 2012-09-26 2014-04-09 浙江大学 一种植物表达载体及培育低植酸水稻的方法
US9783817B2 (en) * 2013-03-04 2017-10-10 Arkansas State University Methods of expressing and detecting activity of expansin in plant cells
CN105050421A (zh) * 2013-03-11 2015-11-11 美盛有限公司 包含饲料酶的粒状饲料磷酸盐组合物
CN107236717B (zh) 2016-03-28 2021-03-30 青岛蔚蓝生物集团有限公司 植酸酶突变体
CA3042046A1 (en) 2016-11-08 2018-05-17 Agrivida, Inc. Phytase production and methods of using the same
CN107353327A (zh) * 2017-03-30 2017-11-17 南京百斯杰生物工程有限公司 植酸酶在黑曲霉中表达
AU2019384024B2 (en) 2018-11-20 2026-01-08 International N&H Denmark Aps Engineered robust high Tm-phytase clade polypeptides and fragments thereof
BR112022017034A2 (pt) 2020-02-28 2022-12-13 Dupont Nutrition Biosci Aps Composições de ração
CN114317488B (zh) * 2021-12-17 2024-07-26 贵州六盘水厚全生态农业有限公司 一种比活力提高的植酸酶突变体
CN115725649A (zh) * 2022-10-17 2023-03-03 浙江大学 一种同时表达两种植酸酶基因的载体及应用
WO2025128743A1 (en) * 2023-12-11 2025-06-19 Lanzatech, Inc. Single cell protein with enhanced digestive enzyme content

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6358722B1 (en) * 1994-04-25 2002-03-19 Roche Vitamins, Inc. Heat tolerant phytases
NZ330940A (en) 1997-07-24 2000-02-28 F Production of consensus phytases from fungal origin using computer programmes
US5876997A (en) * 1997-08-13 1999-03-02 Diversa Corporation Phytase
US6720014B1 (en) * 1997-08-13 2004-04-13 Diversa Corporation Phytase-containing foodstuffs and methods of making and using them
US6855365B2 (en) * 1997-08-13 2005-02-15 Diversa Corporation Recombinant bacterial phytases and uses thereof
US20040091968A1 (en) * 1997-08-13 2004-05-13 Short Jay M. Recombinant phytases and methods of making and using them
US6451572B1 (en) * 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
EP1165806B1 (en) * 1999-03-31 2005-08-03 Cornell Research Foundation, Inc. Phosphatases with improved phytase activity
WO2002048332A2 (en) * 2000-12-12 2002-06-20 Diversa Corporation Recombinant phytases and uses thereof

Also Published As

Publication number Publication date
US20070092555A1 (en) 2007-04-26
US20030170293A1 (en) 2003-09-11
AU2002364919A1 (en) 2003-07-24
CN1622825A (zh) 2005-06-01
TW200305430A (en) 2003-11-01
US7317138B2 (en) 2008-01-08
US7169595B2 (en) 2007-01-30
ZA200405088B (en) 2005-08-10
US7081563B2 (en) 2006-07-25
CA2471859A1 (en) 2003-07-17
MXPA04006389A (es) 2005-07-13
EP1474165A1 (en) 2004-11-10
US7141717B2 (en) 2006-11-28
BR0215406A (pt) 2004-11-09
EP1474165A4 (en) 2005-06-15
US20060137038A1 (en) 2006-06-22
US20060228400A1 (en) 2006-10-12
WO2003057248A1 (en) 2003-07-17

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