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AR036376A1 - A METHOD OF ALTERATION OF THE SENSITIVITY OF A POLYPEPTIDE OF XILANASA TO AN INHIBITOR, AND OF ALTERATION OF THE THERMAL SENSITIVITY OF SUCH POLYPEPTIDE OF XILANASA - Google Patents

A METHOD OF ALTERATION OF THE SENSITIVITY OF A POLYPEPTIDE OF XILANASA TO AN INHIBITOR, AND OF ALTERATION OF THE THERMAL SENSITIVITY OF SUCH POLYPEPTIDE OF XILANASA

Info

Publication number
AR036376A1
AR036376A1 ARP020103232A AR036376A1 AR 036376 A1 AR036376 A1 AR 036376A1 AR P020103232 A ARP020103232 A AR P020103232A AR 036376 A1 AR036376 A1 AR 036376A1
Authority
AR
Argentina
Prior art keywords
polypeptide
sensitivity
xilanasa
alteration
xylanase
Prior art date
Application number
Other languages
Spanish (es)
Original Assignee
Danisco
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco filed Critical Danisco
Publication of AR036376A1 publication Critical patent/AR036376A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente se relaciona con un polipéptido de xilanasa variante, o fragmento del mismo que tiene actividad de xilanasa, que comprende una o más modificaciones de aminoácidos de manera tal que el polipéptido o fragmento del mismo tiene una alterada sensibilidad a un inhibidor de xilanasa y tiene una alterada sensibilidad térmica, comparadas con la enzima xilanasa madre. Se proporciona secuencia nucleótidos que codifica dicho polipéptido variante y construcción de la misma. El campo de aplicación del polipéptido variante es el de ser útil en las áreas de horneado, procesamiento de cereales, producción de almidón, procesamiento de madera, en el realce del blanqueo de pulpa de madera.This is related to a variant xylanase polypeptide, or fragment thereof that has xylanase activity, which comprises one or more amino acid modifications such that the polypeptide or fragment thereof has an altered sensitivity to a xylanase inhibitor and has an altered thermal sensitivity, compared to the mother xylanase enzyme. Nucleotide sequence encoding said variant polypeptide and construction thereof is provided. The field of application of the variant polypeptide is to be useful in the areas of baking, cereal processing, starch production, wood processing, in the enhancement of wood pulp bleaching.

ARP020103232 2001-09-04 2002-08-28 A METHOD OF ALTERATION OF THE SENSITIVITY OF A POLYPEPTIDE OF XILANASA TO AN INHIBITOR, AND OF ALTERATION OF THE THERMAL SENSITIVITY OF SUCH POLYPEPTIDE OF XILANASA AR036376A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB0121387A GB0121387D0 (en) 2001-09-04 2001-09-04 Modified hydrolases

Publications (1)

Publication Number Publication Date
AR036376A1 true AR036376A1 (en) 2004-09-01

Family

ID=9921487

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP020103232 AR036376A1 (en) 2001-09-04 2002-08-28 A METHOD OF ALTERATION OF THE SENSITIVITY OF A POLYPEPTIDE OF XILANASA TO AN INHIBITOR, AND OF ALTERATION OF THE THERMAL SENSITIVITY OF SUCH POLYPEPTIDE OF XILANASA

Country Status (3)

Country Link
AR (1) AR036376A1 (en)
GB (1) GB0121387D0 (en)
WO (1) WO2003020923A1 (en)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6406723B1 (en) 1997-04-09 2002-06-18 Danisco A/S Method for preparing flour doughs and products made from such doughs using glycerol oxidase and lipase
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
MXPA03010511A (en) 2001-05-18 2004-03-02 Danisco Method of improving dough and bread quality.
AU2008201402B2 (en) * 2002-06-14 2012-08-02 Bp Corporation North America Inc. Xylanases, nucleic acids encoding them and methods for making and using them
EA200500019A1 (en) 2002-06-14 2006-06-30 Дайверса Корпорейшн XYLANASE, ENCODING THEIR NUCLEIC ACIDS AND METHODS OF THEIR RECEIVING AND APPLICATION
MXPA05007654A (en) 2003-01-17 2005-09-30 Danisco Method.
US8361529B2 (en) * 2004-02-20 2013-01-29 Novozymes A/S Xylanase
GB0405637D0 (en) 2004-03-12 2004-04-21 Danisco Protein
JP5604032B2 (en) 2004-07-16 2014-10-08 デュポン ニュートリション バイオサイエンシーズ エーピーエス Method for enzymatic degumming of edible oil
EP2548956A1 (en) 2006-02-14 2013-01-23 Verenium Corporation Xylanases, nucleic acids encoding them and methods for making and using them
FR2905825B1 (en) * 2006-09-20 2008-12-26 Lesaffre Et Cie Sa BREADING IMPROVER AND ITS USE IN PLANTING FLAT BREAD WITHOUT THIN
MX2010003600A (en) 2007-10-03 2010-12-14 Verenium Corp Xylanases, nucleic acids encoding them and methods for making and using them.
DK2382310T3 (en) 2008-12-23 2016-12-12 Dupont Nutrition Biosci Aps POLYPEPTIDES HAVING xylanase activity
MX2011006645A (en) * 2008-12-23 2011-09-06 Danisco Polypeptides with xylanase activity.
EA201170938A1 (en) 2009-01-16 2012-06-29 Даниско А/С Enzymatic preparation of functional lipids from cereal or by-product of cereal processing
AU2010233935B2 (en) 2009-03-31 2013-12-12 International N&H Denmark Aps Prevention of extract darkening and malodor formation during solubilization of plant cell wall material
CN102459581B (en) 2009-06-25 2014-10-29 杜邦营养生物科学有限公司 Protein
WO2011114251A1 (en) 2010-03-18 2011-09-22 Danisco A/S Foodstuff
BR112015030655A2 (en) * 2013-06-05 2017-08-22 Novozymes As Xylanase Variants and Polynucleotides Encoding Them
DK3161133T3 (en) 2014-06-25 2019-03-25 Novozymes As Xylanase variants and polynucleotides encoding them
CN116179517B (en) * 2022-07-15 2025-02-11 江苏科技大学 A kind of glucanase mutant and its application
CN120843484B (en) * 2025-08-06 2025-12-23 合肥工业大学 Beta-1, 4-xylanase mutant and construction method thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4226528A1 (en) * 1992-08-11 1994-02-17 Roehm Gmbh Batteryl xylanase, process for their preparation and a suitable bacterial strain, plasmid with associated structural gene, and baking and baking processes for the production of bread and baked goods using the xylanase
JP3435946B2 (en) * 1994-12-21 2003-08-11 王子製紙株式会社 Thermostable xylanase
BRPI9916507B1 (en) * 1998-12-23 2015-09-08 Danisco use of bacterial xylanase to produce a bakery product or dough for preparation thereof and use of an amino acid sequence
JP2002543791A (en) * 1999-05-12 2002-12-24 ゼンコー A new thermostable alkaline xylanase
BR0108750A (en) * 2000-03-08 2002-12-24 Danisco Xylanase variants that have altered sensitivity to xylanase inhibitors

Also Published As

Publication number Publication date
WO2003020923A1 (en) 2003-03-13
GB0121387D0 (en) 2001-10-24

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