AR028807A1 - Procedimiento para aumentar la produccion de cisteina, metionina y celulas vegetales y glutation en plantas, sobreexpresando las enzimas sat de cloroplastos, proteinas de fusion de peptidos de transito que incluyen dichas enzimas sat, secuencias de acidos nucleicos que codifican proteinas de fusion, - Google Patents
Procedimiento para aumentar la produccion de cisteina, metionina y celulas vegetales y glutation en plantas, sobreexpresando las enzimas sat de cloroplastos, proteinas de fusion de peptidos de transito que incluyen dichas enzimas sat, secuencias de acidos nucleicos que codifican proteinas de fusion,Info
- Publication number
- AR028807A1 AR028807A1 ARP990106473A ARP990106473A AR028807A1 AR 028807 A1 AR028807 A1 AR 028807A1 AR P990106473 A ARP990106473 A AR P990106473A AR P990106473 A ARP990106473 A AR P990106473A AR 028807 A1 AR028807 A1 AR 028807A1
- Authority
- AR
- Argentina
- Prior art keywords
- enzymes
- satin
- fusion
- cysteine
- plants
- Prior art date
Links
- 108090000790 Enzymes Proteins 0.000 title abstract 4
- 102000004190 Enzymes Human genes 0.000 title abstract 4
- 210000003763 chloroplast Anatomy 0.000 title abstract 3
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 title abstract 3
- 235000018417 cysteine Nutrition 0.000 title abstract 3
- 210000004027 cell Anatomy 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 230000004927 fusion Effects 0.000 title 2
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 title 1
- 102000004196 processed proteins & peptides Human genes 0.000 title 1
- 108090000765 processed proteins & peptides Proteins 0.000 title 1
- 235000018102 proteins Nutrition 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 235000013311 vegetables Nutrition 0.000 title 1
- 108091022908 Serine O-acetyltransferase Proteins 0.000 abstract 6
- 230000001580 bacterial effect Effects 0.000 abstract 2
- 230000001086 cytosolic effect Effects 0.000 abstract 1
- 238000002703 mutagenesis Methods 0.000 abstract 1
- 231100000350 mutagenesis Toxicity 0.000 abstract 1
- 230000002018 overexpression Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
- C12N15/8253—Methionine or cysteine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
- C12N15/8254—Tryptophan or lysine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Nutrition Science (AREA)
- Medicinal Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Se describe y reivindica un procedimiento para incrementar la produccion de cisteína, en células vegetales y plantas, mediante la sobreexpresion de las enzimas SAT (Serina acetiltransferasa) en los cloroplastos de células vegetales y en las plantas que incluyen dichos cloroplastos, por ejemplo sobreexpresando las enzimas SAT sensibles a la cisteína (SAT cloroplástica vegetales (SAT1)) de origen bacteriano o enzimas SAT insensibles a la cisteína, tal como la SAT citoplasmática vegetal (SAT3), de origen bacteriano o de plantas mutadas, insensibilizadas por mutagénesis. También, se describen y reivindican los objetos restantes, mencionados en el título.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9816163A FR2787466B1 (fr) | 1998-12-17 | 1998-12-17 | Procede pour augmenter la teneur en cysteine, methionine et glutathion chez les plantes et plantes obtenues |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR028807A1 true AR028807A1 (es) | 2003-05-28 |
Family
ID=9534264
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP990106473A AR028807A1 (es) | 1998-12-17 | 1999-12-16 | Procedimiento para aumentar la produccion de cisteina, metionina y celulas vegetales y glutation en plantas, sobreexpresando las enzimas sat de cloroplastos, proteinas de fusion de peptidos de transito que incluyen dichas enzimas sat, secuencias de acidos nucleicos que codifican proteinas de fusion, |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP1141349A1 (es) |
| AR (1) | AR028807A1 (es) |
| AU (1) | AU773031B2 (es) |
| BR (1) | BR9916968A (es) |
| CA (1) | CA2355255A1 (es) |
| FR (1) | FR2787466B1 (es) |
| WO (1) | WO2000036127A1 (es) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69930270T2 (de) | 1998-07-07 | 2006-11-30 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Verfahren zur erhöhung des gehaltes an schwefelverbindungen in pflanzen |
| US6576819B1 (en) | 1999-02-18 | 2003-06-10 | Pioneer Hi-Bred International, Inc. | Methods for modulating the levels of organic sulfur compounds in plants by transforming with (P)APS reductase DNA |
| DE10104721B4 (de) * | 2001-02-02 | 2006-02-09 | IPK-Institut für Pflanzengenetik und Kulturpflanzenforschung | Verfahren zur Erhöhung des Gehalts von Schwefelverbindungen in Pflanzen |
| US7038109B1 (en) | 2001-03-29 | 2006-05-02 | Pioneer Hi-Bred International, Inc. | Enzymatic methods for modulating the levels of organic sulfur compounds in plants |
| CA2382363C (en) | 2001-05-21 | 2012-12-04 | E.I. Du Pont De Nemours And Company | Serine o-acetyl transferase |
| FR2844142B1 (fr) | 2002-09-11 | 2007-08-17 | Bayer Cropscience Sa | Plantes transformees a biosynthese de prenylquinones amelioree |
| FR2848570B1 (fr) | 2002-12-12 | 2005-04-01 | Bayer Cropscience Sa | Cassette d'expression codant pour une 5-enol pyruvylshikimate-3-phosphate synthase (epsps) et plantes tolerantes aux herbicides la contenant |
| DE10260871A1 (de) * | 2002-12-23 | 2004-07-08 | Sungene Gmbh & Co. Kgaa | Verfahren zur Herstellung von transgenen Pflanzen mit erhöhtem Vitamin E-Gehalt durch Veränderung des Serin-Acetyltransferase-Gehalts |
| AR074941A1 (es) | 2009-01-07 | 2011-02-23 | Bayer Cropscience Sa | Plantas transplastomicas exentas de marcador de seleccion |
| PL389131A1 (pl) * | 2009-09-25 | 2011-03-28 | Instytut Biochemii I Biofizyki, Polska Akademia Nauk | Rekombinowana molekuła DNA, wektor, komórka oraz materiał roślinny dostarczający duże ilości enzymatycznie aktywnej syntazy cysteinowej, oraz ich zastosowanie |
| AU2011212538B2 (en) | 2010-02-02 | 2014-12-04 | BASF Agricultural Solutions Seed US LLC | Soybean transformation using HPPD inhibitors as selection agents |
| WO2012029727A1 (ja) * | 2010-08-31 | 2012-03-08 | 独立行政法人科学技術振興機構 | 光合成産物の生産性を向上させた藻類およびその利用 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19539952A1 (de) * | 1995-10-26 | 1997-04-30 | Consortium Elektrochem Ind | Verfahren zur Herstellung von O-Acetylserin, L-Cystein und L-Cystein-verwandten Produkten |
| EP1959016A3 (en) * | 1997-06-06 | 2008-11-05 | E.I. Du Pont De Nemours And Company | Plant amino acid biosynthetic enzymes |
| US6338966B1 (en) * | 1998-07-14 | 2002-01-15 | E. I. Du Pont De Nemours & Company | Genes encoding sulfate assimilation proteins |
-
1998
- 1998-12-17 FR FR9816163A patent/FR2787466B1/fr not_active Expired - Fee Related
-
1999
- 1999-12-16 AR ARP990106473A patent/AR028807A1/es not_active Application Discontinuation
- 1999-12-17 WO PCT/FR1999/003179 patent/WO2000036127A1/fr not_active Ceased
- 1999-12-17 EP EP99961099A patent/EP1141349A1/fr not_active Withdrawn
- 1999-12-17 CA CA002355255A patent/CA2355255A1/en not_active Abandoned
- 1999-12-17 BR BR9916968-1A patent/BR9916968A/pt not_active IP Right Cessation
- 1999-12-17 AU AU17830/00A patent/AU773031B2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| CA2355255A1 (en) | 2000-06-22 |
| AU1783000A (en) | 2000-07-03 |
| FR2787466B1 (fr) | 2001-02-16 |
| BR9916968A (pt) | 2002-01-08 |
| FR2787466A1 (fr) | 2000-06-23 |
| AU773031B2 (en) | 2004-05-13 |
| EP1141349A1 (fr) | 2001-10-10 |
| WO2000036127A1 (fr) | 2000-06-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA | Abandonment or withdrawal |