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AR016256A1 - METHOD FOR INCREASING THE FORGAGE QUALITY OF A PLANT - Google Patents

METHOD FOR INCREASING THE FORGAGE QUALITY OF A PLANT

Info

Publication number
AR016256A1
AR016256A1 ARP990102089A AR016256A1 AR 016256 A1 AR016256 A1 AR 016256A1 AR P990102089 A ARP990102089 A AR P990102089A AR 016256 A1 AR016256 A1 AR 016256A1
Authority
AR
Argentina
Prior art keywords
plant
protein
tissue
high levels
bodies
Prior art date
Application number
Other languages
Spanish (es)
Original Assignee
Univ New Mexico
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ New Mexico filed Critical Univ New Mexico
Publication of AR016256A1 publication Critical patent/AR016256A1/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254Tryptophan or lysine
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/425Zeins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8253Methionine or cysteine

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Fodder In General (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Un método para incrementar la calidad de forraje de una planta, que comprende transformar una planta o tejido de planta con una molécula de polinucleotidosque codifica una proteína de almacenamiento que es expresada y acumulada como un cuerpoproteic o en un tejido vegetal de dicha planta o tejido de planta.Métodos relacionados con plantas y tejidos de plantas transformados, que son capaces de expresar altos niveles de proteínas estables que se ubican como cuerposproteicos dentro de lacélula de la planta. Las plantas transformadas obtenidas co-expresan altos niveles tanto de proteínas zeína de 15 kD como de 10 kD quese acumulan en altos niveles como cuerpos proteicos en el tejido vegetal de la planta. Las plantas transformadasobtenidas so n utiles para proporcionar cultivosde forraje que contienen incrementados niveles de aminoácidosque contienen azufre, tal como metionina, enla dieta de animales que normalmente se alimentan de dichos cultivos. Las plantas transformadaso tejido de pl anta obtenidas comprenden cuerpos proteicosestables que contienen material proteináceo heterologo. Los cuerpos proteicos estables obtenidos son expresados en una planta o tejido de planta como unaproteína de fusion que comprende unaproteína zeína y un péptido o proteína operablemente ligada. Los cuerpos proteicos proporcionados son resistentes a ladigestion del rumen o a la degradacion ambiental.A method for increasing the forage quality of a plant, which comprises transforming a plant or plant tissue with a polynucleotide molecule that encodes a storage protein that is expressed and accumulated as a protein body or in a plant tissue of said plant or tissue of plant.Methods related to plants and tissues of transformed plants, which are capable of expressing high levels of stable proteins that are located as protein bodies within the plant's cell. The transformed plants obtained co-express high levels of both 15 kD and 10 kD zein proteins that accumulate at high levels as protein bodies in the plant's plant tissue. Transformed plants are useful for providing forage crops that contain increased levels of sulfur-containing amino acids, such as methionine, in the diet of animals that normally feed on such crops. The transformed pl tissue plants obtained comprise stable protein bodies that contain heterologous proteinaceous material. The stable protein bodies obtained are expressed in a plant or plant tissue as a fusion protein comprising a zein protein and an operably linked peptide or protein. The protein bodies provided are resistant to rumen digestion or environmental degradation.

ARP990102089 1998-12-31 1999-05-04 METHOD FOR INCREASING THE FORGAGE QUALITY OF A PLANT AR016256A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US22465598A 1998-12-31 1998-12-31

Publications (1)

Publication Number Publication Date
AR016256A1 true AR016256A1 (en) 2001-06-20

Family

ID=22841589

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP990102089 AR016256A1 (en) 1998-12-31 1999-05-04 METHOD FOR INCREASING THE FORGAGE QUALITY OF A PLANT

Country Status (8)

Country Link
EP (1) EP1141347A1 (en)
JP (1) JP2002541768A (en)
CN (1) CN1334877A (en)
AR (1) AR016256A1 (en)
AU (1) AU3652299A (en)
BR (1) BR9916731A (en)
CA (1) CA2358548A1 (en)
WO (1) WO2000040738A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2224792B1 (en) * 2002-06-28 2007-02-16 Era Plantech, S.L. PRODUCTION OF PEPTIDES AND PROTEINS BY ACCUMULATION OF PROTEIN BODIES DERIVED FROM ENDOPLASMIC RETICLES IN PLANTS.
AR047658A1 (en) 2004-02-03 2006-02-01 Cargill Inc CONCENTRATE OF PROTEINS AND WATER CURRENT WITH HYDROSOLUBBLE CARBOHYDRATES
US9555097B2 (en) 2006-02-23 2017-01-31 Era Biotech, S.A. Recombinant protein bodies as immunogen-specific adjuvants
EP2418284A1 (en) 2010-08-13 2012-02-15 ERA Biotech, S.A. Protein body-inducing polypeptide sequences
CN103261216A (en) * 2010-12-10 2013-08-21 巴斯夫植物科学有限公司 Modified protein body tags and production methods thereof
JP2015131781A (en) * 2014-01-14 2015-07-23 カタギ食品株式会社 Polyclonal antibody, kit for detecting sesame allergen, method for determining low-allergen sesame, method for producing low-allergen sesame, and low-allergen sesame

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2744134B1 (en) * 1996-01-29 1998-04-03 Biocem RESERVE PROTEINS FROM PLANTS ENRICHED IN AMINO ACIDS, IN PARTICULAR ALPHA-ZEIN BUT ENRICHED IN LYSINE PLANTS EXPRESSING SUCH PROTEINS

Also Published As

Publication number Publication date
BR9916731A (en) 2001-11-06
AU3652299A (en) 2000-07-24
WO2000040738A1 (en) 2000-07-13
JP2002541768A (en) 2002-12-10
CN1334877A (en) 2002-02-06
CA2358548A1 (en) 2000-07-13
EP1141347A1 (en) 2001-10-10

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