AR008570A1 - Materiales recombinantes y metodos para la produccion de hidroxilasas de limoneno - Google Patents
Materiales recombinantes y metodos para la produccion de hidroxilasas de limonenoInfo
- Publication number
- AR008570A1 AR008570A1 ARP980102999A ARP980102999A AR008570A1 AR 008570 A1 AR008570 A1 AR 008570A1 AR P980102999 A ARP980102999 A AR P980102999A AR P980102999 A ARP980102999 A AR P980102999A AR 008570 A1 AR008570 A1 AR 008570A1
- Authority
- AR
- Argentina
- Prior art keywords
- hydroxylase
- limonene
- expression
- recombinant
- production
- Prior art date
Links
- 108010074633 Mixed Function Oxygenases Proteins 0.000 title abstract 3
- 102000008109 Mixed Function Oxygenases Human genes 0.000 title abstract 3
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 235000010290 biphenyl Nutrition 0.000 title 1
- ZUOUZKKEUPVFJK-UHFFFAOYSA-N diphenyl Chemical compound C1=CC=CC=C1C1=CC=CC=C1 ZUOUZKKEUPVFJK-UHFFFAOYSA-N 0.000 title 1
- 101710084031 Cytochrome P450 71D180 Proteins 0.000 abstract 8
- 101710084033 Cytochrome P450 71D181 Proteins 0.000 abstract 8
- 101710154745 Limonene hydroxylase Proteins 0.000 abstract 8
- 235000014749 Mentha crispa Nutrition 0.000 abstract 3
- 244000078639 Mentha spicata Species 0.000 abstract 3
- NOOLISFMXDJSKH-KXUCPTDWSA-N (-)-Menthol Chemical compound CC(C)[C@@H]1CC[C@@H](C)C[C@H]1O NOOLISFMXDJSKH-KXUCPTDWSA-N 0.000 abstract 2
- XMGQYMWWDOXHJM-SNVBAGLBSA-N (-)-α-limonene Chemical compound CC(=C)[C@H]1CCC(C)=CC1 XMGQYMWWDOXHJM-SNVBAGLBSA-N 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 2
- 238000010367 cloning Methods 0.000 abstract 2
- 230000000295 complement effect Effects 0.000 abstract 2
- 108010006833 limonene-6-hydroxylase Proteins 0.000 abstract 2
- 229960003595 (-)- limonene Drugs 0.000 abstract 1
- NOOLISFMXDJSKH-UHFFFAOYSA-N DL-menthol Natural products CC(C)C1CCC(C)CC1O NOOLISFMXDJSKH-UHFFFAOYSA-N 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 abstract 1
- 235000004357 Mentha x piperita Nutrition 0.000 abstract 1
- 241001479543 Mentha x piperita Species 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 230000008260 defense mechanism Effects 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 108010008618 limonene-3-hydroxylase Proteins 0.000 abstract 1
- 239000001771 mentha piperita Substances 0.000 abstract 1
- 239000001220 mentha spicata Substances 0.000 abstract 1
- 229940041616 menthol Drugs 0.000 abstract 1
- 230000001717 pathogenic effect Effects 0.000 abstract 1
- 238000003752 polymerase chain reaction Methods 0.000 abstract 1
- 244000062645 predators Species 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 238000003259 recombinant expression Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2799/00—Uses of viruses
- C12N2799/02—Uses of viruses as vector
- C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
- C12N2799/026—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a baculovirus
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
(-)-limoneno-6-hidroxilasa de menta verde y (-)-limoneno de hidroxilasa de mentol que codifican sDNA se aislaron y secuenciaron, y se determinaron lassecuencias aminoácidas correspondientes. De la misma manera, se proporcionan secuencias de DNAa islado que cifran la expresion de (-)-limoneno-6-hidroxilasade menta verde (ID SEC N*1, Mentha spicata) y (-)-limoneno-3-hidroxilasa de mentol (ID SEC N*8, de Mentha piperita). En otros aspectos, se proporcionanvehículos de clonacionrecombinante s replicables que cifran la hidroxilasa de limoneno o una secuencia base suficientemente complementaria para al menos unaporcion de DNA o RNA de hidroxilasa de limoneno para permitir la hibridacion (por ejemplo, RNA de hidroxilasade limoneno de contasentido o fragmentos deDDNA de hidroxilasa de limoneno complementario que resultan utiles como indicadores de reaccion en cadena de polimerasa o sondas para hidroxilasa delimoneno o genes relacionados. En aun otrosaspectos, se proporci onan células huésped modificadas que han sido transformadas, infectadas y/o inyectadas conun vehículo de clonacion recombinante y/o una secuencia de DNA que codifica hidroxilasa de limoneno. De esta manera, seproporcionan sistemas y métodospar a la expresion recombinante de hidroxilasa de limoneno que se pueden utilizar para facilitar la produccion, aislamiento y purificacion de cantidadessignificativas de hidroxilasa de limoneno recombinante (ode productos enzimáticos pr imarios, trans-carveol o trans-isopiperitenol, respectivamente)para su consiguiente uso, con el fin de obtener la expresion o la expresion aumentada de hidroxilasa de limoneno en plantas con el objeto delograr unaproduccion aumentada de trans-ca rveol o trans-isopiperitenol como predador o mecanismo de defensa patogeno, o que pueden utilizarse también para la
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/881,784 US6083731A (en) | 1997-06-24 | 1997-06-24 | Recombinant materials and methods for the production of limonene hydroxylases |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR008570A1 true AR008570A1 (es) | 2000-02-09 |
Family
ID=25379207
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP980102999A AR008570A1 (es) | 1997-06-24 | 1998-06-23 | Materiales recombinantes y metodos para la produccion de hidroxilasas de limoneno |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US6083731A (es) |
| EP (1) | EP1017792A4 (es) |
| AR (1) | AR008570A1 (es) |
| AU (1) | AU8147898A (es) |
| CA (1) | CA2294475C (es) |
| WO (1) | WO1998059042A1 (es) |
| ZA (1) | ZA984820B (es) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030228600A1 (en) * | 2000-07-14 | 2003-12-11 | Eppendorf 5 Prime, Inc. | DNA isolation method and kit |
| ES2284666T3 (es) * | 2000-07-21 | 2007-11-16 | E.I. Du Pont De Nemours And Company | Una enzima de citocromo p450 asociada con la sintesis de grupos delta-12-epoxi en acidos grasos de plantas. |
| EP1205556A1 (en) * | 2000-11-10 | 2002-05-15 | Aventis Animal Nutrition S.A. | Process for the preparation of trans-carveol |
| EP1236802A1 (en) * | 2001-02-16 | 2002-09-04 | Aventis Animal Nutrition S.A. | Process for the preparation of perillyl alcohol |
| JP2004532014A (ja) | 2001-03-09 | 2004-10-21 | ユニバーシティ・オブ・ケンタッキー・リサーチ・ファウンデーション | チトクロームｐ４５０類及びその使用 |
| AU2003256731A1 (en) * | 2002-08-02 | 2004-02-23 | Aromagen Corporation | Methods for making (-) -menthol and oxygenated menthane compounds |
| ES2784011T3 (es) | 2002-10-16 | 2020-09-21 | Streck Inc | Procedimiento y dispositivo para recoger y preservar las células para su análisis |
| SI1919514T1 (sl) | 2005-07-05 | 2012-08-31 | Univ California | Polinukleotidi, ki kodirajo encime, ki modificirajo izoprenoid, in postopki za njihovo uporabo |
| SG182157A1 (en) | 2007-06-01 | 2012-07-30 | Solazyme Inc | Production of oil in microorganisms |
| AP2741A (en) * | 2007-07-05 | 2013-09-30 | Univ California | Polynucleotides encoding isoprenoid modifying enzymes and methods of use thereof |
| AU2009319721B2 (en) | 2008-11-28 | 2016-01-14 | Corbion Biotech, Inc. | Manufacturing of tailored oils in recombinant heterotrophic microorganisms |
| US11634747B2 (en) | 2009-01-21 | 2023-04-25 | Streck Llc | Preservation of fetal nucleic acids in maternal plasma |
| EP3778919A1 (en) * | 2009-02-18 | 2021-02-17 | Streck Inc. | Preservation of cell-free nucleic acids |
| AU2011257982B2 (en) | 2010-05-28 | 2017-05-25 | Corbion Biotech, Inc. | Tailored oils produced from recombinant heterotrophic microorganisms |
| CN103827307A (zh) | 2011-02-02 | 2014-05-28 | 索拉兹米公司 | 产自重组产油微生物的定制油 |
| US9545075B2 (en) | 2011-02-08 | 2017-01-17 | Kemin Industries, Inc. | Spearmint plant denominated KI-MsEM0110 |
| US20150013026A1 (en) * | 2011-02-08 | 2015-01-08 | Kemin Industries, Inc. | SPEARMINT PLANT MENTHA SPICATA L. DENOMINATED KI-MsEM0110 |
| EP2704740B1 (en) | 2011-05-04 | 2016-10-05 | Streck, Inc. | Inactivated swine flu virus and methods of preparing it |
| CA2917912C (en) | 2013-07-24 | 2019-09-17 | Streck, Inc. | Compositions and methods for stabilizing circulating tumor cells |
| US11168351B2 (en) | 2015-03-05 | 2021-11-09 | Streck, Inc. | Stabilization of nucleic acids in urine |
| US20170145475A1 (en) | 2015-11-20 | 2017-05-25 | Streck, Inc. | Single spin process for blood plasma separation and plasma composition including preservative |
| US11506655B2 (en) | 2016-07-29 | 2022-11-22 | Streck, Inc. | Suspension composition for hematology analysis control |
| EP3697209B1 (en) | 2017-10-19 | 2024-07-24 | Streck LLC | Compositions for hemolysis and coagulation regulation and stabilization of extracellular vesicles |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993022441A1 (en) * | 1992-04-23 | 1993-11-11 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Dna sequence of a plant resistance gene |
-
1997
- 1997-06-24 US US08/881,784 patent/US6083731A/en not_active Expired - Lifetime
-
1998
- 1998-06-04 ZA ZA984820A patent/ZA984820B/xx unknown
- 1998-06-15 EP EP98931324A patent/EP1017792A4/en not_active Withdrawn
- 1998-06-15 WO PCT/US1998/012581 patent/WO1998059042A1/en not_active Ceased
- 1998-06-15 AU AU81478/98A patent/AU8147898A/en not_active Abandoned
- 1998-06-15 CA CA002294475A patent/CA2294475C/en not_active Expired - Fee Related
- 1998-06-23 AR ARP980102999A patent/AR008570A1/es not_active Application Discontinuation
-
1999
- 1999-04-14 US US09/292,768 patent/US6194185B1/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP1017792A1 (en) | 2000-07-12 |
| ZA984820B (en) | 1999-01-04 |
| US6083731A (en) | 2000-07-04 |
| EP1017792A4 (en) | 2005-04-20 |
| AU8147898A (en) | 1999-01-04 |
| CA2294475A1 (en) | 1998-12-30 |
| WO1998059042A1 (en) | 1998-12-30 |
| CA2294475C (en) | 2004-11-09 |
| US6194185B1 (en) | 2001-02-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA | Abandonment or withdrawal |