[go: up one dir, main page]

AP2011005669A0 - Primer and amplification method for forming singlestranded DNA amplicons. - Google Patents

Primer and amplification method for forming singlestranded DNA amplicons.

Info

Publication number
AP2011005669A0
AP2011005669A0 AP2011005669A AP2011005669A AP2011005669A0 AP 2011005669 A0 AP2011005669 A0 AP 2011005669A0 AP 2011005669 A AP2011005669 A AP 2011005669A AP 2011005669 A AP2011005669 A AP 2011005669A AP 2011005669 A0 AP2011005669 A0 AP 2011005669A0
Authority
AP
ARIPO
Prior art keywords
primer
forming
amplification method
dna amplicons
singlestranded dna
Prior art date
Application number
AP2011005669A
Inventor
Alexio Capovilla
Natela Rekhviashvili
Natasha Gous
Original Assignee
Univ Witwatersrand Jhb
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Witwatersrand Jhb filed Critical Univ Witwatersrand Jhb
Publication of AP2011005669A0 publication Critical patent/AP2011005669A0/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AP2011005669A 2008-09-15 2009-09-15 Primer and amplification method for forming singlestranded DNA amplicons. AP2011005669A0 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ZA200802591 2008-09-15
PCT/IB2009/054027 WO2010029520A1 (en) 2008-09-15 2009-09-15 Primer and amplification method for forming single stranded dna amplicons

Publications (1)

Publication Number Publication Date
AP2011005669A0 true AP2011005669A0 (en) 2011-04-30

Family

ID=41510654

Family Applications (1)

Application Number Title Priority Date Filing Date
AP2011005669A AP2011005669A0 (en) 2008-09-15 2009-09-15 Primer and amplification method for forming singlestranded DNA amplicons.

Country Status (3)

Country Link
AP (1) AP2011005669A0 (en)
WO (1) WO2010029520A1 (en)
ZA (1) ZA201102807B (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016106129A2 (en) 2014-12-23 2016-06-30 Ge Healthcare Uk Limited Methods and reagents for reverse-transcription polymerase chain reaction
WO2016106113A1 (en) 2014-12-23 2016-06-30 Ge Healthcare Uk Limited Methods and reagents for reverse-transcription polymerase chain reaction
WO2016106111A1 (en) 2014-12-23 2016-06-30 Ge Healthcare Uk Limited Methods and reagents for reverse-transcription polymerase chain reaction
US10870879B2 (en) 2015-10-05 2020-12-22 Helmholtz Zentrum Münchendeutsches Forschungszentrum Für Gesundheit Und Umwelt Method for the preparation of bar-coded primer sets

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5916779A (en) * 1995-09-21 1999-06-29 Becton, Dickinson And Company Strand displacement amplification of RNA targets
WO2006082496A2 (en) * 2005-02-01 2006-08-10 National Health Laboratory Service Method for determining hiv-1 viral load
JP2010505438A (en) * 2006-10-09 2010-02-25 オキシテック・リミテッド Methods for amplifying and detecting nucleic acid sequences
CN101605911A (en) * 2007-01-17 2009-12-16 梅里第安生物科学公司 Stable reagents and kits effective for loop-mediated isothermal amplification (LAMP) of nucleic acids

Also Published As

Publication number Publication date
ZA201102807B (en) 2012-07-25
WO2010029520A1 (en) 2010-03-18

Similar Documents

Publication Publication Date Title
GB2470672B (en) Methods of RNA amplification in the presence of DNA
EP2058396A4 (en) Nucleic acid amplification method
GB0713143D0 (en) Nucleic acid detection method
ZA201103357B (en) Microfluidic apparatus and method for dna extraction, amplification and analysis
EP2126119A4 (en) Apparatus and method for nucleic acid amplification
EP2354226A4 (en) Method for detecting controls for nucleic acid amplification, and use thereof
AP2010005520A0 (en) Floatation reagents and floatation processes utilizing same.
EP2431465A4 (en) Method for synthesis of double-stranded dna corresponding to rna, and method for amplification of the dna
EP2367958A4 (en) Compositions and methods for detecting small rnas, and uses thereof
ZA201008749B (en) Isothermal nucleic acid amplification
BRPI0913292A2 (en) hydroconversion method, and hydroconversion reaction zone.
GB2460996B8 (en) Combustion method.
PT2326411E (en) Extraction system, and related method
EP2292798A4 (en) Rna in situ hybridization
EP2380679A4 (en) Forging method
GB0812862D0 (en) Nucleic acid amplification
EP2208795A4 (en) Nucleic acid detection method, and nucleic acid detection kit
GB0917615D0 (en) Method for increasing the speed of nucleic acid amplification reactions
EP2396497A4 (en) Method for using retainer, and retainer
EP2216406A4 (en) Carrier, method and reagent for obtaining small rna
EP2195453A4 (en) Method of amplifying nucleic acid
EP2210169A4 (en) Characters input method
AP2011005669A0 (en) Primer and amplification method for forming singlestranded DNA amplicons.
EP2157187A4 (en) Convection polymerase chain reaction method
EP1929011A4 (en) Nucleic acid extraction method