NZ709157B2 - Methods and comp0stions for treatment of demyelinating diseases - Google Patents

Abstract

Disclosed herein are new oral pharmaceutical compositions of SAMDC inhibitors, polyamine analogs, and polyamine biosynthesis inhibitors, and their application for the treatment of conditions including demyelinating diseases, autoimmune disorders affecting the nervous system, and other neurodegenerative conditions. Specifically, the use of methylglyoxal bis(guanylhydrazone) (MGBG) in the manufacture of a medicament for the treatment of progressive multiple sclerosis is claimed. The medicament may be formulated for co-administration with fingolimod. ive conditions. Specifically, the use of methylglyoxal bis(guanylhydrazone) (MGBG) in the manufacture of a medicament for the treatment of progressive multiple sclerosis is claimed. The medicament may be formulated for co-administration with fingolimod.

Description

METHODS AND COMPOSITIONS FOR TREATMENT OF DEMYELINATING DISEASES This application claims the benefit of priority of United States provisional applications no. 61/750,336, filed January 8, 2013, and no. 61/823,276, filed May 14, 2013. New Zealand Patent ation No. 749754 is a divisional application out of the t application and the disclosures of these applications are incorporated by reference as if written herein in their entireties.

Disclosed herein are new oral pharmaceutical compositions of SAMDC inhibitors, polyamine analogs, and polyamine biosynthesis inhibitors, and their application for the treatment of conditions ing demyelinating diseases, autoimmune disorders affecting the nervous system, and other neurodegenerative conditions.

MGBG lglyoxal bis(guanylhydrazone); mitoguazone) is a itive polyamine inhibitor of osyl methionine decarboxylase (SAMDC, AMD-I), which catalyzes the synthesis of spermidine, a polyamine. The amino-acid-derived polyamines have long been associated with cell growth and cancer, and specific oncogenes and tumor-suppressor genes regulate ine metabolism. Inhibition of polyamine synthesis has proven to be generally ineffective as an anticancer strategy in clinical trials, but it is a potent cancer chemoprevention strategy in preclinical studies. Despite its novel mechanism of action and promising nical data, initial clinical trials of MGBG were ceased in the middle of 1960s due to severe toxicity especially to self-renewing normal tissues such as the bone marrow and intestinal tract (particularly severe mucositis), which were both dose and schedule dependent.

Regardless, research has continued with MGBG. A number of studies have examined potential uses in ation with other chemotherapeutic agents and tive dosing regimens, designed to minimize side effects and dose where possible. Others have focused on elucidating MGBG's modes of action in the body. Yet others have igated MGBG's activity in diseases other than .

Perhaps due to negative clinical findings in these early studies, to date, MGBG had been confined to intravenous use. As a practical matter, this presents a number of problems for treatment of many diseases, particularly of c or recurrent conditions. stration via IV injection or infusion must be done by a medical professional in a hospital setting. This not only presents an inconvenience and increased cost to the subject, but it also exposes him or her to al-based infections and illnesses, this latter both from venipuncture and the al or clinic visit itself. In immunocompromised individuals, individuals undergoing treatment with immune system suppressors, and the elderly, this is a relevant concern. Thus, a subject with a long-term chronic condition such as an autoimmune or hyperproliferative er, or a doctor treating such a subject, might find the cost, inconvenience, and risks of such a treatment more important than any potential eutic benefits the drug might offer.

Additionally, I.V. infusion drugs suffer from high Cmax and Tmax liabilities as opposed to oral drugs, which are more slowly absorbed.

An oral formulation of MGBG, in contrast, would present several benefits. First, an oral ation, for example a simple pill or tablet, may be taken outside of a hospital setting, increasing the potential for subject ease of use and compliance. This permits a subject to avoid infection risks itant with IV administration and hospital visits.

Where early treatment can prevent the development of disease complications, this is of particular benefit. Chronic low-dose administration of MGBG is practically impossible in an IV ation. Additionally, oral delivery lly avoids the high concentration peak and rapid clearance associated with an IV bolus dose. Yet another age of an oral drug would be the ability to formulate MGBG as a combination composition with one or more other therapeutic agents.

SUMMARY OF THE INVENTION [006A] In a particular aspect, the present invention provides the use of MGBG in the manufacture of a medicament for treatment of progressive multiple sclerosis in a patient.

BRIEF DESCRIPTION OF THE DRAWINGS shows the mean clinical scores of subjects in the first 28-day murine model of experimental autoimmune encephalomyelitis (EAE) (a chronic ssive model of MS) induced by inoculation with myelin oligodendrocyte glycoprotein (MOG) peptides, wherein test subjects were dosed with vehicle, 30 mpk MGBG (twice-daily), or 3 mpk fingolimod (once-daily), and ed with mock-immunized subjects. (followed by page 2a) shows the mean percentage change in body weight ive to study start) of subjects in the first 28-day MOG EAE model, wherein test subjects were dosed with e, mpk MGBG (twice-daily), or 3 mpk fingolimod (once-daily), and compared with mockimmunized subjects. shows the reduction in spinal cord inflammation as measured by the number of inflammatory foci in spinal cord histopathological samples, from the 28-day MOG EAE model, wherein test ts were dosed with vehicle, 30 mpk MGBG (twice-daily), or 3 mpk fingolimod (once-daily), and compared with mock-immunized subjects. shows reduction in spinal cord demyelination in histopathological samples from the 28-day MOG EAE model, wherein test subjects were dosed with vehicle, 30 mpk [FOLLOWED BY PAGE 3] MGBG (twice-daily), or 3 mpk fingolimod (once-daily), and compared with mock— immunized subjects. shows reduction in cellular apoptosis in spinal cord histopathological samples from the 28-day MOG EAE model, wherein test subjects were dosed with vehicle, mpk MGBG (twice-daily), or 3 mpk imod (once-daily), and compared with mock- immunized subjects. shows the mean clinical scores of ts in the second 28-day murine MOG EAE model, wherein test subjects were dosed with vehicle, 30 mpk MGBG (twice- daily), or 1 mpk fingolimod (once-daily), and compared with mock-immunized subjects; MGBG suppressed EAE comparably to 1 mpk fingolimod. shows the mean percentage change in body weight (relative to study start) of subjects in the second 28-day MOG EAE model, wherein test subjects were dosed with vehicle, 30 mpk MGBG (twice-daily), or 1 mpk imod (once-daily), and compared with mock-immunized subjects. shows that MGBG, but not fingolimod, reduces CNS A) CD11c+ dendritic cells associated with antigen presentation and B) IL12+ dendritic cells associated with promotion of the Th1 response. shows that MGBG, but not fingolimod, reduces A) Th1 and B) M1 macrophages. shows that fingolimod preferentially A) sses Thl7 and B) ses M2 macrophages; thus, MGBG and fingolimod have distinct, but therapeutically complementary activities in MS. shows that although plasma levels of MGBG are almost undetectable by 28 hours after a single dose in the rat, MGBG levels in the spleen and liver remain detectably higher even 48 hours post-dose. This is consistent with a ive uptake ism for MGBG. shows the daily mean al scores of subjects in the third 28—day murine MOG EAE model, where fingolimod was dosed at 0.1 mpk (once-daily); MGBG dosed at 30 mpk (twice-daily) ssed EAE comparably to 0.1 mpk fingolimod, and the combination of MGBG and fingolimod entirely suppressed development of EAE throughout the course of the study (no animals developed disease with the combination therapy). , showing the cumulative mean clinical scores of subjects in the third EAE study, further illustrates the significance of the above results. MGBG at 30 mpk (twice-daily) and fingolimod at 0.1 mpk (once-daily) were equivalently efficacious in preventing EAE development, and the ation of the two entirely suppressed development of EAE. shows the change in body weight ive to study start) of subjects in the third 28-day EAE model, corroborating the results in . shows the ion in spinal cord ation as measured by the number of inflammatory foci in spinal cord histopathological samples, from the third EAE model. Again, MGBG at 30 mpk (twice-daily) and fingolimod at 0.1 mpk (once-daily) were equivalently efficacious in ng the number of inflammatory foci, and the combination of the two apparently suppressed their development completely. shows the reduction in cellular apoptosis in spinal cord histopathological samples from the third EAE model. Again, MGBG at 30 mpk -daily) and imod at 0.1 mpk (once—daily) were equivalently efficacious in reducing apoptosis, and the combination of the two apparently suppressed sis completely. shows reduction in spinal cord demyelination in histopathological samples from the third EAE model. Again, MGBG at 30 mpk (twice-daily) and fingolimod at 0.1 mpk (once—daily) were equivalently efficacious in reducing demyelination, and the combination of the two apparently prevented ination completely.

Accordingly, provided herein is a method of treatment or prevention of a demyelinating disease, or a symptom thereof, comprising the administration of a therapeutically effective amount of a SAMDC inhibitor.

In certain embodiments, the SAMDC inhibitor is selectively uptaken into cells.

In certain embodiments, the SAMDC inhibitor is MGBG.

In certain embodiments, the administration of MGBG is oral.

In certain embodiments, MGBG is dosed at 20 mg/day to 400 mg/day.

In n embodiments, the demyelinating disease is chosen from multiple sclerosis, optic neuritis, an thic inflammatory demyelinating disease, Guillain-Barré Syndrome, chronic inflammatory demyelinating polyneuropathy, transverse myelitis, Balo concentric sclerosis, pernicious anemia, central pontine olysis, Tabes is, neuromyelitis optica (NMO), progressive multifocal leukoencephalopathy (PML), anti-MAG (myelin-associated glycoprotein) neuropathy, hereditary motor and y neuropathy (Chacot-Marie-Tooth disease), cerebrotendinious xanthanomatosis, and leukodystrophies including adrenoleukodystrophy, adrenomyeloneuropathy, metachromatic leukodystrophy, WO 10154 globoid cell leukodystrophy (Krabbe disease), Canavan disease, vanishing white matter disease, Alexander disease, Refsum disease, and Pelizaeus-Merzbacher disease.

In certain ments, the demyelinating disease is le sclerosis.

In certain embodiments, the method additionally comprises the administration of an agent chosen from interferon a, interferon beta-lb, glatiramer acetate, mitoxantrone, natalizumab, fingolimod, laquinimod, dimethyl fumarate, and teriflunomide.

In certain embodiments, the agent is fingolimod.

In certain embodiments, fingolimod is dosed at 0.5 mg per day.

In certain embodiments, imod is dosed at less than 0.5 mg per day.

In certain embodiments, fingolimod is dosed at 0.25 mg per day. [03 6] Also provided herein are ments in which each of the embodiments above in paragraphs [0024] — [0035] is combined with one or more of the other non-contradictory embodiments, such that the resulting ment includes the two or more recited elements and/or limitations.

Also provided herein is a method of treatment of a symptom of an autoimmune disease affecting the nervous system comprising the administration of a therapeutically effective amount of a SAMDC inhibitor. [03 8] In certain embodiments, the SAMDC inhibitor is selectively uptaken into cells. [03 9] In certain embodiments, the SAMDC inhibitor is MGBG.

In certain embodiments, the administration of MGBG is oral.

In certain embodiments, MGBG is dosed at 20 mg/day to 400 mg/day.

In certain embodiments, the symptom is chosen from CNS inflammation, demyelination, and paralysis.

In certain embodiments, the autoimmune disease affecting the nervous system is chosen from le sclerosis, polymyalgia, enia gravis, Guillain-Barré Syndrome, chronic atory demyelinating polyneuropathy, erse is, Balo tric sclerosis, ious anemia, acute disseminated encephalomyelitis (ADME), amyotrophic lateral sis (ALS), autoimmune peripheral neuropathy, lupus erythematosus, psoriatic arthritis, rheumatoid arthritis, osteoarthritis, and rheumatic fever.

In certain embodiments, the autoimmune disease affecting the nervous system disease is multiple sclerosis.

In certain embodiments, the method additionally comprises the administration of an agent chosen from interferon beta-la, eron beta-lb, glatiramer acetate, mitoxantrone, natalizumab, fingolimod, laquinimod, dimethyl fumarate, and teriflunomide.

In certain embodiments, the agent is fingolimod.

In certain embodiments, fingolimod is dosed at 0.5 mg per day.

In certain embodiments, fingolimod is dosed at less than 0.5 mg per day.

In certain embodiments, fingolimod is dosed at 0.25 mg per day.

Also provided herein are ments in which each of the embodiments above in aphs [0037] — [0049] is combined with one or more of the other non—contradictory embodiments, such that the resulting ment includes the two or more recited elements and/or limitations.

Also provided herein is a method of treatment or prevention of a demyelinating disease comprising the contemporaneous administration of a SAMDC tor and an agent chosen from interferon beta-la, interferon beta-lb, glatiramer acetate, mitoxantrone, natalizumab, fingolimod, yl fumarate, and nomide.

In n embodiments, the other agent is fingolimod.

In certain embodiments, the SAMDC inhibitor is selectively uptaken into cells.

In certain embodiments, the SAMDC inhibitor is MGBG.

In certain embodiments, the administration of MGBG is oral. [05 6] In certain embodiments, MGBG is dosed at 20 mg/day to 400 mg/day.

In certain embodiments, the demyelinating disease is chosen from multiple sclerosis, Guillain—B arré Syndrome, chronic inflammatory demyelinating polyneuropathy, transverse is, Balo concentric sclerosis, pernicious anemia, central pontine myelinolysis, Tabes is, neuromyelitis optica (NMO), progressive multifocal leukoencephalopathy (PML), anti-MAG (myelin-associated glycoprotein) neuropathy, hereditary motor and sensory neuropathy t-Man'e-Tooth disease), cerebrotendinious xanthanomatosis, and leukodystrophies including adrenoleukodystrophy, adrenomyeloneuropathy, romatic leukodystrophy, globoid cell leukodystrophy (Krabbe disease), Canavan disease, vanishing white matter disease, Alexander disease, Refsum disease, and Pelizaeus-Merzbacher disease.

In certain embodiments, the demyelinating disease is multiple sclerosis. [05 9] In n embodiments, fingolimod is dosed at 0.5 mg per day.

In certain embodiments, fingolimod is dosed at less than 0.5 mg per day.

In certain embodiments, fingolimod is dosed at 0.25 mg per day.

Also provided herein are embodiments in which each of the ments above in aphs [0051] — [0061] is combined with one or more of the other non-contradictory embodiments, such that the resulting embodiment includes the two or more recited elements and/or limitations.

Also provided herein is a pharmaceutical composition comprising a SAMDC inhibitor and another agent chosen from interferon beta-la, interferon b, glatiramer acetate, mitoxantrone, natalizumab, fingolimod, and teriflunomide, er with a pharmaceutically able carrier.

In certain embodiments, the SAMDC inhibitor is selectively uptaken into cells.

In certain embodiments, the SAMDC inhibitor is MGBG.

In n embodiments, MGBG is dosed at 20 mg/day to 400 mg/day.

In certain embodiments, the other agent is fingolimod.

In certain embodiments, fingolimod is dosed at 0.5 mg per day.

In certain embodiments, fingolimod is dosed at less than 0.5 mg per day.

In certain embodiments, fingolimod is dosed at 0.25 mg per day.

In certain ments, the pharmaceutical formulation sing the SAMDC inhibitor and fingolimod is formulated for oral administration.

In certain embodiments, the pharmaceutical formulation yields a therapeutically effective systemic plasma MGBG level when orally administered to a subject.

In certain embodiments, the pharmaceutical formulation is formulated for once- daily dosing.

In n embodiments, the pharmaceutical formulation comprises 0.5 mg fingolimod per dosage unit.

In n embodiments, the pharmaceutical formulation comprises less than 0.5 mg fingolimod per dosage unit.

In n embodiments, the pharmaceutical formulation comprises 0.25 mg fingolimod per dosage unit.

In certain embodiments, the pharmaceutical formulation is formulated for twice- daily dosing.

In n embodiments, the pharmaceutical ation comprises 0.25 mg fingolimod per dosage unit.

In certain embodiments, the pharmaceutical formulation comprises less than 0.25 mg fingolimod per dosage unit.

In certain embodiments, the pharmaceutical formulation comprises about 0.125 mg fingolimod per dosage unit.

Also provided herein are embodiments in which each of the embodiments above in paragraphs [0063] — [0080] is combined with one or more of the other non-contradictory ments, such that the resulting ment includes the two or more recited elements and/or limitations.

Also provided is a method of prevention of relapse or progression, or decreasing severity of symptoms in a relapse, of a demyelinating disease in a t, comprising the administration of a therapeutically effective amount of a SAMDC inhibitor.

In certain embodiments, provided is a method of prevention of progression, or sing severity of symptoms in a relapse, of a demyelinating disease in a patient, comprising the administration of a therapeutically effective amount of MGBG.

In certain embodiments, the demyelinating disease is chosen from multiple sclerosis, optic neuritis, an thic inflammatory inating disease, Guillain-Barre Syndrome, chronic inflammatory demyelinating polyneuropathy, transverse myelitis, Balo concentric sclerosis, pernicious anemia, central pontine myelinolysis, Tabes dorsalis, neuromyelitis optica (NMO), progressive multifocal leukoencephalopathy (PML), anti-MAG (myelin-associated glycoprotein) neuropathy, hereditary motor and sensory neuropathy (Chacot-Marie-Tooth e), cerebrotendinious xanthanomatosis, and leukodystrophies including adrenoleukodystrophy, adrenomyeloneuropathy, metachromatic leukodystrophy, globoid cell leukodystrophy (Krabbe disease), Canavan disease, vanishing white matter disease, Alexander disease, Refsum disease, and Pelizaeus—Merzbacher disease.

In certain embodiments, the demyelinating disease is le sclerosis.

In certain embodiments, the SAMDC inhibitor is selectively uptaken into cells.

In certain embodiments, the SAMDC inhibitor is MGBG.

In certain ments, the administration of MGBG is oral.

In certain embodiments, MGBG is dosed at 20 mg/day to 400 mg/day.

In n embodiments, the SAMDC inhibitor is not a T-cell regulator.

In n embodiments, stration occurs concomitant with a d incidence of at least one side effect chosen from cytopenia, nephrotoxicity, hepatotoxicity, cardiotoxicity, teratogenicity, decreased pulmonary function, macular edema, peripheral athy, severe skin reactions, increased risk of infections (including latent bacterial and viral), impairment of innate immunity, impairment of adaptive immunity, and flushing, as compared to another therapeutic agent approved for the treatment of a inating disease.

In n embodiments, the demyelinating disease is multiple sclerosis.

In certain embodiments, the method additionally comprises the administration of an agent chosen from interferon beta-1a, interferon beta-1b, glatiramer acetate, mitoxantrone, natalizumab, fingolimod, laquinimod, dimethyl fumarate, and teriflunomide.

In certain embodiments, the agent is fingolimod.

In certain embodiments, fingolimod is dosed at 0.5 mg per day.

In certain embodiments, fingolimod is dosed at less than 0.5 mg per day.

In certain embodiments, fingolimod is dosed at 0.25 mg per day.

In n ments, administration occurs concomitant with a reduced incidence of at least one side effect chosen from cytopenia, nephrotoxicity, hepatotoxicity, cardiotoxicity, and teratogenicity.

In certain embodiments, the cytopenia is chosen from lymphopenia and neutropenia.

In certain embodiments, administration occurs concomitant with a reduced incidence of at least two side s chosen from nia, nephrotoxicity, hepatotoxicity, cardiotoxicity, and teratogenicity.

In certain embodiments, the cytopenia is chosen from lymphopenia and neutropenia.

In certain embodiments, administration occurs concomitant with a reduced nce of cytopenia, nephrotoxicity, and hepatotoxicity.

In certain ments, the cytopenia is chosen from lymphopenia and neutropenia.

In certain embodiments, administration additionally occurs concomitant with a reduced incidence of toxicity, and teratogenicity.

Also ed herein are embodiments in which each of the embodiments above in paragraphs [0082] — [0104] is combined with one or more of the other non-contradictory embodiments, such that the resulting embodiment includes the two or more recited elements and/or limitations.

Also provided is a method treatment of progressive multiple sclerosis in a patient, comprising the stration of a therapeutically effective amount of a SAMDC inhibitor.

In certain embodiments, the SAMDC inhibitor is selectively uptaken into cells.

In certain embodiments, the SAMDC inhibitor is MGBG.

In certain embodiments, the ssive multiple sclerosis is primary progressive.

In certain ments, the progressive multiple sclerosis is ary progressive.

In certain embodiments, the progressive multiple sis is progressive ing.

In certain embodiments, the administration of MGBG is oral.

In certain embodiments, MGBG is dosed at 20 mg/day to 400 mg/day.

In n embodiments, the method additionally comprises the administration of an agent chosen from interferon beta—1a, interferon beta-1b, glatiramer acetate, mitoxantrone, natalizumab, fingolimod, laquinimod, dimethyl fumarate, and teriflunomide.

In certain embodiments, the agent is fingolimod.

In certain embodiments, fingolimod is dosed at 0.5 mg per day.

In certain ments, imod is dosed at less than 0.5 mg per day.

In certain embodiments, fingolimod is dosed at 0.25 mg per day.

In certain embodiments, the treatment prevents relapse or ssion of MS.

In certain embodiments, the SAMDC inhibitor is not a T-cell regulator.

In certain ments, administration occurs concomitant with a reduced nce of at least one side effect chosen from cytopenia, nephrotoxicity, hepatotoxicity, toxicity, teratogenicity, decreased pulmonary function, macular edema, peripheral neuropathy, severe skin reactions, increased risk of infections (including latent bacterial and viral), impairment of innate ty, impairment of adaptive immunity, and flushing, as ed to another therapeutic agent approved for the treatment of a demyelinating disease.

In certain embodiments, the demyelinating disease is multiple sclerosis.

In certain embodiments, administration occurs concomitant with a reduced incidence of at least one side effect chosen from cytopenia, nephrotoxicity, hepatotoxicity, toxicity, and teratogenicity.

In certain embodiments, the nia is chosen from lymphopenia and neutropenia.

In n embodiments, administration occurs concomitant with a reduced incidence of at least two side effects chosen from cytopenia, nephrotoxicity, hepatotoxicity, cardiotoxicity, and teratogenicity.

In certain embodiments, the cytopenia is chosen from lymphopenia and neutropenia.

In certain embodiments, administration occurs concomitant with a reduced incidence of cytopenia, nephrotoxicity, and hepatotoxicity.

In certain embodiments, the cytopenia is chosen from lymphopenia and neutropenia.

In certain embodiments, administration additionally occurs concomitant with a reduced incidence of cardiotoxicity, and teratogenicity.

Also ed herein are embodiments in which each of the embodiments above in paragraphs [0106] — [0129] is combined with one or more of the other ntradictory embodiments, such that the resulting embodiment includes the two or more recited elements and/or limitations.

Also provided is a method of blocking antigen presentation on cells in a patient having a demyelinating e, wherein the antigens are derived from, mimic, or resemble antigens in the myelin sheath, comprising the administration of a therapeutically effective amount of a SAMDC inhibitor.

In certain embodiments, the cells are of myeloid lineage.

In n embodiments, the cells are pro-inflammatory.

In certain embodiments, the cells are chosen from dendritic cells, macrophages, and B-cells.

In certain ments, the cells are dendritic cells.

In certain embodiments, the cells are M1 macrophages.

In certain embodiments, the demyelinating disease is chosen from multiple sclerosis, optic neuritis, an idiopathic inflammatory demyelinating disease, Guillain—Barre Syndrome, chronic inflammatory demyelinating polyneuropathy, transverse is, Balo concentric sis, pernicious anemia, central pontine myelinolysis, Tabes dorsalis, neuromyelitis optica (NMO), progressive ocal leukoencephalopathy (PML), anti-MAG n-associated glycoprotein) neuropathy, hereditary motor and sensory neuropathy (Chacot-Marie-Tooth disease), cerebrotendinious xanthanomatosis, and leukodystrophies including adrenoleukodystrophy, adrenomyeloneuropathy, metachromatic leukodystrophy, globoid cell leukodystrophy e disease), Canavan disease, vanishing white matter disease, Alexander disease, Refsum e, and Pelizaeus-Merzbacher disease.

In certain embodiments, the demyelinating disease is multiple sclerosis.

In certain embodiments, the method additionally comprises the stration of an agent chosen from interferon beta-1a, interferon beta-1b, glatiramer acetate, ntrone, natalizumab, imod, laquinimod, dimethyl fumarate, and teriflunomide.

In certain embodiments, the agent is fingolimod.

In certain embodiments, imod is dosed at 0.5 mg per day.

In certain embodiments, fingolimod is dosed at less than 0.5 mg per day.

In certain embodiments, fingolimod is dosed at 0.25 mg per day.

WO 10154 In certain embodiments, the SAMDC inhibitor is not a T-cell regulator.

In n embodiments, the SAMDC inhibitor is MGBG.

In n embodiments, the administration of MGBG is oral.

In certain embodiments, MGBG is dosed at 20 mg/day to 400 mg/day.

In certain embodiments, administration occurs concomitant with a reduced incidence of at least one side effect chosen from cytopenia, nephrotoxicity, hepatotoxicity, cardiotoxicity, teratogenicity, decreased pulmonary function, r edema, peripheral neuropathy, severe skin reactions, increased risk of infections (including latent bacterial and viral), impairment of innate immunity, ment of adaptive immunity, and flushing, as compared to another therapeutic agent approved for the treatment of a demyelinating disease.

In certain embodiments, the inating disease is multiple sclerosis.

In n embodiments, administration occurs concomitant with a reduced incidence of at least one side effect chosen from cytopenia, nephrotoxicity, toxicity, cardiotoxicity, and teratogenicity.

In certain embodiments, the cytopenia is chosen from lymphopenia and neutropenia.

In certain embodiments, administration occurs concomitant with a reduced incidence of at least two side effects chosen from cytopenia, nephrotoxicity, hepatotoxicity, cardiotoxicity, and teratogenicity.

In certain ments, the cytopenia is chosen from lymphopenia and neutropenia.

In certain embodiments, administration occurs concomitant with a d incidence of cytopenia, nephrotoxicity, and hepatotoxicity.

In certain embodiments, the cytopenia is chosen from lymphopenia and neutropenia.

In n embodiments, administration additionally occurs concomitant with a reduced incidence of cardiotoxicity, and genicity.

Also provided herein are embodiments in which each of the embodiments above in paragraphs [0131] — [0156] is combined with one or more of the other non-contradictory embodiments, such that the resulting embodiment includes the two or more d elements and/or limitations.

Also provided is a method of inhibition of infiltration of an antigen-presenting cells into the central nervous system of a t having a demyelinating disease, comprising the administration of a therapeutically effective amount of a SAMDC inhibitor.

In certain embodiments, the cells are flammatory.

In certain embodiments, the cells are chosen from dendritic cells, hages, and s.

In certain embodiments, the cells are dendritic cells.

In certain embodiments, the cells are M1 macrophages.

In certain ments, the SAMDC inhibitor is selectively n into cells.

The method as recited in any one of claims 76-80, wherein the SAMDC inhibitor is MGBG.

In certain embodiments, the administration of MGBG is oral.

In certain embodiments, MGBG is dosed at 20 mg/day to 400 mg/day.

In certain embodiments, the method additionally comprises the administration of an agent chosen from interferon beta-1a, interferon beta-1b, glatiramer acetate, mitoxantrone, natalizumab, fingolimod, laquinimod, dimethyl fumarate, and teriflunomide.

In certain embodiments, the agent is fingolimod.

In certain embodiments, fingolimod is dosed at 0.5 mg per day.

In certain ments, fingolimod is dosed at less than 0.5 mg per day.

In certain embodiments, fingolimod is dosed at 0.25 mg per day.

In certain ments, administration occurs concomitant with a reduced incidence of at least one side effect chosen from cytopenia, nephrotoxicity, hepatotoxicity, toxicity, teratogenicity, sed pulmonary function, macular edema, peripheral neuropathy, severe skin reactions, increased risk of infections (including latent bacterial and viral), impairment of innate immunity, impairment of adaptive immunity, and flushing, as compared to another therapeutic agent approved for the treatment of a demyelinating disease.

In certain embodiments, the demyelinating disease is multiple sclerosis.

In certain embodiments, administration occurs itant with a reduced incidence of at least one side effect chosen from nia, nephrotoxicity, hepatotoxicity, cardiotoxicity, and teratogenicity.

In certain embodiments, the cytopenia is chosen from lymphopenia and neutropenia.

In certain embodiments, administration occurs concomitant with a reduced incidence of at least two side effects chosen from cytopenia, toxicity, hepatotoxicity, cardiotoxicity, and teratogenicity.

In certain embodiments, the cytopenia is chosen from lymphopenia and neutropenia.

In certain embodiments, administration occurs concomitant with a reduced incidence of cytopenia, nephrotoxicity, and hepatotoxicity.

In certain embodiments, the cytopenia is chosen from lymphopenia and neutropenia.

In certain ments, administration additionally occurs concomitant with a reduced incidence of cardiotoxicity, and genicity.

Also ed herein are embodiments in which each of the embodiments above in aphs [0158] — [0180] is combined with one or more of the other non-contradictory embodiments, such that the resulting embodiment includes the two or more recited ts and/or limitations.

Also provided is a method of prevention or reduction in severity of the initiation phase of autoimmune response in a patient having a demyelinating disease, comprising the administration of a therapeutically effective amount of a SAMDC inhibitor.

In certain embodiments, the administration onally prevents or reduces the amplification phase of autoimmune response in a patient having a demyelinating disease.

In certain embodiments, the demyelinating disease is chosen from multiple sclerosis, optic neuritis, an thic inflammatory demyelinating disease, Guillain—Barré me, chronic inflammatory demyelinating polyneuropathy, transverse myelitis, Balo concentric sclerosis, ious anemia, central pontine myelinolysis, Tabes dorsalis, neuromyelitis optica (NMO), progressive multifocal leukoencephalopathy (PML), anti-MAG (myelin-associated glycoprotein) neuropathy, hereditary motor and sensory neuropathy t-Marie-Tooth disease), cerebrotendinious xanthanomatosis, and leukodystrophies ing adrenoleukodystrophy, adrenomyeloneuropathy, metachromatic leukodystrophy, globoid cell leukodystrophy (Krabbe disease), Canavan e, vanishing white matter disease, Alexander disease, Refsum disease, and Pelizaeus-Merzbacher e.

In certain embodiments, the demyelinating disease is multiple sclerosis.

In certain embodiments, the SAMDC inhibitor is not a T-cell regulator.

In certain embodiments, the SAMDC inhibitor is MGBG.

In certain embodiments, the administration of MGBG is oral.

In certain embodiments, MGBG is dosed at 20 mg/day to 400 mg/day.

In certain ments, the method additionally comprises the administration of an agent chosen from interferon beta-1a, eron beta-1b, glatiramer acetate, mitoxantrone, natalizumab, fingolimod, imod, dimethyl fumarate, and teriflunomide.

In certain embodiments, the agent is fingolimod. 2014/010714 In certain embodiments, fingolimod is dosed at 0.5 mg per day.

In n ments, imod is dosed at less than 0.5 mg per day.

In certain embodiments, fingolimod is dosed at 0.25 mg per day.

In certain embodiments, administration occurs concomitant with a reduced incidence of at least one side effect chosen from cytopenia, nephrotoxicity, hepatotoxicity, cardiotoxicity, teratogenicity, decreased pulmonary function, macular edema, eral neuropathy, severe skin reactions, increased risk of infections (including latent bacterial and viral), impairment of innate immunity, impairment of adaptive immunity, and flushing, as compared to another therapeutic agent approved for the treatment of a demyelinating disease.

In certain embodiments, the demyelinating disease is multiple sclerosis.

In certain embodiments, administration occurs concomitant with a reduced incidence of at least one side effect chosen from cytopenia, nephrotoxicity, hepatotoxicity, cardiotoxicity, and teratogenicity.

In certain embodiments, the cytopenia is chosen from penia and neutropenia.

In certain embodiments, administration occurs concomitant with a reduced incidence of at least two side effects chosen from cytopenia, nephrotoxicity, hepatotoxicity, cardiotoxicity, and teratogenicity.

In certain embodiments, the cytopenia is chosen from lymphopenia and neutropenia.

In certain embodiments, stration occurs concomitant with a reduced incidence of cytopenia, nephrotoxicity, and hepatotoxicity.

In certain embodiments, the cytopenia is chosen from penia and neutropenia.

In certain embodiments, administration additionally occurs concomitant with a reduced incidence of cardiotoxicity, and teratogenicity.

Also provided herein are ments in which each of the ments above in paragraphs [0182] — [0203] is combined with one or more of the other non-contradictory embodiments, such that the resulting embodiment includes the two or more recited ts and/or limitations.

Also ed is a method of treatment or prevention of demyelination in a subject in need thereof, comprising the administration of a therapeutically effective amount of a SAMDC inhibitor.

In n embodiments, the SAMDC inhibitor is selectively uptaken into cells.

In certain embodiments, the SAMDC inhibitor is MGBG.

In certain embodiments, the administration of MGBG is oral.

In certain embodiments, MGBG is dosed at 20 mg/day to 400 .

In certain embodiments, the demyelinating disease is chosen from multiple sclerosis, optic neuritis, an idiopathic inflammatory demyelinating e, Guillain—Barre me, chronic inflammatory demyelinating polyneuropathy, transverse is, Balo concentric sclerosis, pernicious anemia, central pontine myelinolysis, Tabes dorsalis, neuromyelitis optica (NMO), progressive multifocal leukoencephalopathy (PML), anti-MAG (myelin-associated glycoprotein) neuropathy, hereditary motor and sensory neuropathy (Chacot-Marie-Tooth disease), cerebrotendinious xanthanomatosis, and leukodystrophies ing adrenoleukodystrophy, adrenomyeloneuropathy, metachromatic leukodystrophy, globoid cell leukodystrophy (Krabbe disease), Canavan disease, vanishing white matter disease, Alexander disease, Refsum e, and Pelizaeus-Merzbacher disease.

In certain embodiments, the demyelinating disease is multiple sclerosis.

In certain embodiments, the method additionally ses the stration of an agent chosen from interferon beta-1a, interferon beta-1b, amer acetate, mitoxantrone, natalizumab, fingolimod, laquinimod, dimethyl fumarate, and teriflunomide.

In certain embodiments, the agent is fingolimod.

In n embodiments, fingolimod is dosed at 0.5 mg per day.

In certain embodiments, fingolimod is dosed at less than 0.5 mg per day.

In certain embodiments, fingolimod is dosed at 0.25 mg per day.

Also provided herein are embodiments in which each of the embodiments above in paragraphs [0205] — [0216] is combined with one or more of the other non-contradictory embodiments, such that the resulting ment includes the two or more recited elements and/or limitations.

Also provided is a method of reduction in severity of cellular sis in the nerve tissue of a t in need thereof, comprising the administration of a therapeutically effective amount of a SAMDC inhibitor.

In certain embodiments, the SAMDC inhibitor is selectively uptaken into cells.

In certain embodiments, the SAMDC inhibitor is MGBG.

In certain embodiments, the administration of MGBG is oral.

In certain embodiments, MGBG is dosed at 20 mg/day to 400 mg/day.

In certain embodiments, the inating disease is chosen from multiple sclerosis, optic neuritis, an thic inflammatory demyelinating disease, Guillain—Barré Syndrome, chronic inflammatory inating polyneuropathy, transverse myelitis, Balo concentric sclerosis, pernicious anemia, central pontine myelinolysis, Tabes is, neuromyelitis optica (NMO), progressive multifocal leukoencephalopathy (PML), anti-MAG n-associated glycoprotein) neuropathy, hereditary motor and sensory neuropathy (Chacot-Marie-Tooth disease), cerebrotendinious xanthanomatosis, and leukodystrophies including adrenoleukodystrophy, adrenomyeloneuropathy, metachromatic ystrophy, globoid cell leukodystrophy (Krabbe disease), Canavan disease, vanishing white matter disease, Alexander disease, Refsum disease, and Pelizaeus-Merzbacher disease.

In certain embodiments, the demyelinating disease is multiple sclerosis.

In certain embodiments, the method additionally comprises the administration of an agent chosen from interferon beta-la, interferon beta-lb, amer acetate, mitoxantrone, zumab, fingolimod, laquinimod, dimethyl fumarate, and nomide.

In certain embodiments, the agent is fingolimod.

In certain embodiments, fingolimod is dosed at 0.5 mg per day.

In certain embodiments, fingolimod is dosed at less than 0.5 mg per day.

In certain embodiments, fingolimod is dosed at 0.25 mg per day.

Also provided herein are embodiments in which each of the embodiments above in paragraphs [0218] — [0229] is combined with one or more of the other ntradictory ments, such that the resulting embodiment includes the two or more recited elements and/or limitations.

Also provided is a method of tion of or reduction in the development of inflammatory foci in the nerve tissue of a subject in need thereof, comprising the administration of a therapeutically ive amount of a SAMDC inhibitor.

In certain embodiments, the SAMDC inhibitor is selectively uptaken into cells.

In certain embodiments, the SAMDC inhibitor is MGBG.

In n embodiments, the administration of MGBG is oral.

In certain embodiments, MGBG is dosed at 20 mg/day to 400 mg/day.

In n embodiments, the demyelinating disease is chosen from multiple sclerosis, optic neuritis, an idiopathic atory demyelinating disease, Guillain-Barré Syndrome, chronic inflammatory demyelinating polyneuropathy, transverse myelitis, Balo concentric sclerosis, pernicious anemia, central pontine myelinolysis, Tabes is, neuromyelitis optica (NMO), ssive multifocal leukoencephalopathy (PML), anti-MAG (myelin-associated glycoprotein) neuropathy, hereditary motor and sensory neuropathy (Chacot-Marie-Tooth disease), cerebrotendinious xanthanomatosis, and leukodystrophies including adrenoleukodystrophy, adrenomyeloneuropathy, metachromatic leukodystrophy, globoid cell ystrophy (Krabbe disease), n disease, vanishing white matter disease, Alexander disease, Refsum e, and Pelizaeus—Merzbacher disease.

In certain embodiments, the demyelinating disease is multiple sclerosis.

In certain embodiments, the method additionally comprises the administration of an agent chosen from interferon beta—1a, interferon beta-1b, glatiramer acetate, mitoxantrone, zumab, fingolimod, laquinimod, dimethyl fumarate, and teriflunomide.

In certain embodiments, the agent is fingolimod.

In certain embodiments, fingolimod is dosed at 0.5 mg per day.

In certain embodiments, imod is dosed at less than 0.5 mg per day.

In certain embodiments, fingolimod is dosed at 0.25 mg per day.

Also provided herein are embodiments in which each of the embodiments above in paragraphs [0231] — [0242] is combined with one or more of the other non-contradictory embodiments, such that the resulting embodiment includes the two or more recited elements and/or limitations.

The present disclosure contemplates not only the methods disclosed above, but also: 0 the corresponding use of the compounds above in the treatment of disease, and in the specific diseases discussed; and 0 the corresponding use of the nds above in the manufacture of medicaments for the treatment of the es discussed.

In the interest of nce of repetition, such embodiments are not explicitly re- n herein. However, these embodiments should be understood to be included as if so written. For example, the present disclosure provides for: 0 Use of a therapeutically effective amount of a SAMDC inhibitor in the treatment or prevention of a demyelinating disease; 0 Use of a eutically effective amount of a SAMDC inhibitor in the treatment of a symptom of an autoimmune e affecting the nervous system; 0 Use of a therapeutically effective amount of a SAMDC inhibitor and an agent chosen from interferon beta-la, interferon beta-1b, amer acetate, mitoxantrone, natalizumab, fingolimod, dimethyl fumarate, and teriflunomide in the treatment or tion of a inating disease; 2014/010714 Use of a therapeutically effective amount of a SAMDC inhibitor in the prevention of relapse or progression, or decreasing severity of symptoms in a relapse, of a demyelinating disease; Use of a therapeutically effective amount of a SAMDC inhibitor in the ent of progressive multiple sclerosis in a patient; Use of a therapeutically effective amount of a SAMDC tor in blocking antigen tation on cells in a patient having a demyelinating disease, wherein the antigens are derived from, mimic, or resemble antigens in the myelin sheath; Use of a therapeutically effective amount of a SAMDC inhibitor in the inhibition of infiltration of an antigen-presenting cells into the central s system of a patient having a demyelinating disease; a method of prevention or reduction in severity of the initiation phase of autoimmune response in a patient having a demyelinating disease, comprising the administration of a therapeutically effective amount of a SAMDC inhibitor.

Use of a therapeutically effective amount of a SAMDC inhibitor in the treatment or prevention of demyelination in a subject in need thereof; Use of a therapeutically effective amount of a SAMDC inhibitor in the reduction in severity of ar apoptosis in the nerve tissue of a subject in need thereof; and/or Use of a therapeutically effective amount of a SAMDC tor in the prevention of or reduction in the development of inflammatory foci in the nerve tissue of a t in need thereof; and all ent ments listed above in paragraphs [0024] — [0242] above and in the claims below. The present disclosure also provides for: Use of a SAMDC inhibitor in the manufacture of a medicament for the treatment or prevention of a demyelinating disease; Use of a SAMDC inhibitor in the manufacture of a medicament for the treatment of a symptom of an autoimmune disease affecting the nervous system; Use of a SAMDC inhibitor and an agent chosen from interferon beta—la, interferon beta-lb, glatiramer acetate, mitoxantrone, natalizumab, fingolimod, dimethyl fumarate, and teriflunomide in the manufacture of a medicament for the ent or prevention of a demyelinating disease; 0 Use of a SAMDC inhibitor in the manufacture of a medicament for the prevention of e or progression, or decreasing severity of symptoms in a relapse, of a demyelinating disease; 0 Use of a SAMDC inhibitor in the manufacture of a medicament for the treatment of progressive multiple sclerosis in a patient; 0 Use of a SAMDC inhibitor in the manufacture of a medicament for blocking antigen presentation on cells in a patient having a demyelinating disease, wherein the antigens are derived from, mimic, or resemble antigens in the myelin sheath; 0 Use of a SAMDC inhibitor in the cture of a medicament for the inhibition of infiltration of an antigen-presenting cells into the central nervous system of a patient having a demyelinating disease; 0 a method of prevention or reduction in the manufacture of a ment for severity of the initiation phase of autoimmune response in a patient having a demyelinating e, comprising the stration of a SAMDC inhibitor. 0 Use of a SAMDC inhibitor in the manufacture of a medicament for the treatment or prevention of demyelination in a subject in need thereof; 0 Use of a SAMDC inhibitor in the manufacture of a medicament for the reduction in severity of cellular apoptosis in the nerve tissue of a subject in need thereof; and/or 0 Use of a SAMDC inhibitor in the manufacture of a medicament for the prevention of or reduction in the development of inflammatory foci in the nerve tissue of a subject in need thereof; and all dependent embodiments listed above in paragraphs [00]24 — [0242] above and in the claims below.

Also disclosed herein are oral pharmaceutical ations of MGBG and other polyamine analogs, polyamine biosynthesis inhibitors, and polyamine inhibitors of SAMDC, Also sed are methods for the treatment of diseases comprising the administration of MGBG and other polyamine s, polyamine biosynthesis tors, and polyamine inhibitors of SAMDC.

Also provided herein are methods for the ent of pain comprising the administration of MGBG and other polyamine analogs, polyamine biosynthesis tors, and ine inhibitors of SAMDC.

Also provided herein is a pharmaceutical composition for oral delivery, comprising a ine analog or polyamine biosynthesis inhibitor together with at least one ceutically acceptable oral excipient.

Also ed herein is an oral pharmaceutical composition, comprising polyamine analog or polyamine biosynthesis inhibitor together with at least one oral pharmaceutically acceptable excipient, which yields a therapeutically effective systemic plasma polyamine analog or polyamine biosynthesis inhibitor level when orally administered to a subject.

Also provided herein is an oral pharmaceutical composition, comprising polyamine analog or polyamine biosynthesis inhibitor er with at least one oral ceutically acceptable excipient, which yields a therapeutically effective systemic plasma ine analog or polyamine biosynthesis inhibitor level for the treatment of pain when orally administered to a subject.

In certain embodiments, the polyamine analog or polyamine biosynthesis inhibitor is a compound disclosed herein.

In certain ments, the polyamine analog or polyamine biosynthesis inhibitor is one known in the art.

In certain embodiments, the pharmaceutical composition yields a therapeutically effective systemic plasma level of a polyamine analog or polyamine biosynthesis inhibitor for aperiod of at least 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 30, 36, or 48 hours. In further embodiments, the pharmaceutical composition yields a therapeutically effective systemic plasma level of a polyamine analog or polyamine biosynthesis inhibitor for at least a 6-hour period. In further ments, the pharmaceutical ition yields a therapeutically effective systemic plasma level of a polyamine analog or polyamine biosynthesis tor for at least a 12-hour period. In further embodiments, the pharmaceutical composition yields a therapeutically effective systemic plasma level of a polyamine analog or polyamine biosynthesis inhibitor for at least an 18- hour period. In further embodiments, the pharmaceutical composition yields a therapeutically effective systemic plasma level of a polyamine analog or polyamine biosynthesis inhibitor for at least a 24-hour period.

In certain embodiments, the pharmaceutical ition yields a plasma level of a polyamine analog or polyamine biosynthesis inhibitor of at least 25, 50, 55, 60, 65, 75, 80, 85, 90, or 95 percent of the peak plasma concentration for at least 4 hours. In certain embodiments, the pharmaceutical ition yields a plasma level of a polyamine analog or polyamine biosynthesis inhibitor of at least 75% of the peak plasma concentration for at least 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, or 24 hours. In certain embodiments, the pharmaceutical composition yields a plasma level of a polyamine analog or polyamine biosynthesis inhibitor of at least 75% of the peak plasma concentration for at least 4 hours. In certain embodiments, the pharmaceutical composition yields a plasma level of a ine analog or ine biosynthesis inhibitor of at least 75% of the peak plasma concentration for at least 6 hours. In certain embodiments, the pharmaceutical composition yields a plasma level of a polyamine analog or ine thesis inhibitor of at least 75% of the peak plasma concentration for at least 8 hours. In certain embodiments, the pharmaceutical composition yields a plasma level of a polyamine analog or polyamine biosynthesis inhibitor of at least 50% of the peak plasma concentration for at least 8 hours. In certain embodiments, the pharmaceutical composition yields a plasma level of a polyamine analog or polyamine biosynthesis inhibitor of at least 50% of the peak plasma concentration for at least 12 hours. In certain embodiments, the pharmaceutical composition yields a plasma level of a polyamine analog or polyamine biosynthesis inhibitor of at least 50% of the peak plasma concentration for at least 18 hours. In certain embodiments, the pharmaceutical composition yields a plasma level of a polyamine analog or polyamine biosynthesis inhibitor of at least 25% of the peak plasma concentration for at least 18 hours. In further embodiments, the peak plasma concentration is a therapeutically effective concentration. In yet further embodiments, the percentage of peak plasma concentration is eutically ive over the given time period.

In certain embodiments, the pharmaceutical ition comprising the polyamine analog or polyamine thesis inhibitor has an oral bioavailability of at least , 20, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 45, 50, 55, or 60 percent.

In further ments, the pharmaceutical composition has an oral bioavailability of at least %, 20%, 25 %, 30%, 35%, 40%, or 45%. In further embodiments, the pharmaceutical composition has an oral ilability of at least 30%, at least 35%, at least 40% or at least 45%. In certain embodiments, the pharmaceutical ition has an oral bioavailability of at least 20%. In certain embodiments, the pharmaceutical composition has an oral bioavailability of at least 30%. In certain embodiments, the pharmaceutical composition has an oral ilability of at least 35%. In certain embodiments, the pharmaceutical composition has an oral bioavailability of at least 40%. In certain embodiments, the pharmaceutical composition has an oral bioavailability of at least 45%. In certain ments, the pharmaceutical composition has an oral bioavailability which yields a therapeutically effective plasma level of a polyamine analog or polyamine biosynthesis 2014/010714 inhibitor for at least a 24 hour period in the subject with once-daily dosing. In certain embodiments, the pharmaceutical composition has an oral bioavailability which yields a therapeutically ive plasma level of a polyamine analog or polyamine thesis inhibitor for at least a 24 hour period in the subject with twice-daily dosing. In certain embodiments, the pharmaceutical composition has an oral bioavailability which yields a eutically ive plasma level of a polyamine analog or polyamine biosynthesis inhibitor for at least a 24 hour period in the subject with thrice-daily dosing.

In certain embodiments, the pharmaceutical composition comprising the polyamine analog or polyamine biosynthesis inhibitor has a half life of at least 4, 6, 8, 10, 12, 14, 16, 18, 19, 20, 21, 22, 23, 24, 26, 28, 30, or 36 hours. In certain embodiments, the pharmaceutical composition has a half life of at least 12 hours. In further embodiments, the pharmaceutical composition has a half life of at least 18 hours. In further embodiments, the pharmaceutical composition has a half life of at least 20 hours. In further embodiments, the pharmaceutical ition has a half life of at least 24 hours. In certain embodiments, the pharmaceutical composition has a half life of at least 48, 72, 96, or 120 hours.

Additionally provided herein is a pharmaceutical composition for oral delivery, comprising MGBG together with at least one ceutically able oral excipient.

Also provided herein is an oral pharmaceutical composition, comprising MGBG together with at least one oral pharmaceutically acceptable excipient, which yields a therapeutically ive systemic plasma MGBG level when orally administered to a subject.

Also provided herein is an oral pharmaceutical composition, sing MGBG together with at least one oral pharmaceutically acceptable excipient, which yields a therapeutically ive systemic plasma MGBG level for the treatment of pain when orally administered to a subject.

In certain embodiments, the pharmaceutical composition yields a therapeutically effective systemic plasma MGBG level for a period of at least 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, l6, l7, l8, 19, 20, 21, 22, 23, 24, 30, 36, or 48 hours. In further embodiments, the pharmaceutical composition yields a therapeutically effective systemic plasma MGBG level for at least a 6-hour period. In further embodiments, the pharmaceutical composition yields a therapeutically effective systemic plasma MGBG level for at least a 12-hour period. In further embodiments, the pharmaceutical ition yields a therapeutically effective systemic plasma MGBG level for at least an 18-hour period. In further embodiments, the 2014/010714 pharmaceutical composition yields a therapeutically effective systemic plasma MGBG level for at least a 24-hour period.

In certain embodiments, the pharmaceutical ition yields a plasma level of MGBG of at least 25, 50, 55, 60, 65, 75, 80, 85, 90, or 95 percent of the peak plasma concentration for at least 4 hours. In certain ments, the pharmaceutical composition yields a plasma level of MGBG of at least 75% of the peak plasma concentration for at least 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, or 24 hours. In certain embodiments, the pharmaceutical composition yields a plasma level of MGBG of at least 75% of the peak plasma concentration for at least 4 hours. In certain embodiments, the pharmaceutical composition yields a plasma level of MGBG of at least 75% of the peak plasma concentration for at least 6 hours. In certain embodiments, the pharmaceutical composition yields a plasma level of MGBG of at least 75% of the peak plasma concentration for at least 8 hours. In n embodiments, the pharmaceutical composition yields a plasma level of MGBG of at least 50% of the peak plasma tration for at least 8 hours. In n embodiments, the pharmaceutical composition yields a plasma level of MGBG of at least 50% of the peak plasma concentration for at least 12 hours. In certain embodiments, the pharmaceutical composition yields a plasma level of MGBG of at least 50% of the peak plasma concentration for at least 18 hours.

In certain embodiments, the pharmaceutical composition yields a plasma level of MGBG of at least 25% of the peak plasma concentration for at least 18 hours. In further embodiments, the peak plasma concentration is a therapeutically effective concentration. In yet further embodiments, the percentage of peak plasma concentration is therapeutically effective over the given time period.

In certain embodiments, the pharmaceutical composition comprising MGBG has an oral bioavailability of at least 10, 20, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 45, 50, 55, or 60 t. In further embodiments, the pharmaceutical composition has an oral bioavailability of at least 10%, 20%, 25%, 30%, 35%, 40%, or 45%. In further embodiments, the pharmaceutical composition has an oral bioavailability of at least 30%, at least 35 %, at least 40% or at least 45 %. In certain embodiments, the pharmaceutical composition has an oral bioavailability of at least 20%. In certain embodiments, the pharmaceutical composition has an oral bioavailability of at least 30%. In certain embodiments, the pharmaceutical composition has an oral ilability of at least 35%. In n embodiments, the pharmaceutical composition has an oral bioavailability of at least 40%. In certain embodiments, the pharmaceutical composition has an oral bioavailability of at least 45%. In n embodiments, the pharmaceutical composition has an oral bioavailability which yields a therapeutically effective plasma level of MGBG for at least a 24 hour period in the subject with once-daily dosing. In certain embodiments, the pharmaceutical composition has an oral bioavailability which yields a therapeutically effective plasma level of MGBG for at least a 24 hour period in the subject with daily dosing. In certain embodiments, the pharmaceutical composition has an oral bioavailability which yields a therapeutically effective plasma level of MGBG for at least a 24 hour period in the subject with thrice-daily dosing.

In certain ments, the pharmaceutical composition comprising MGBG has a half life of at least 4, 6, 8, 10, 12, 14, 16, 18, 19, 20, 21, 22, 23, 24, 26, 28, 30, or 36 hours.

In certain embodiments, the pharmaceutical ition has a half life of at least 12 hours.

In further embodiments, the pharmaceutical composition has a half life of at least 18 hours.

In further embodiments, the ceutical composition has a half life of at least 20 hours.

In further embodiments, the pharmaceutical composition has a half life of at least 24 hours.

In certain embodiments, the pharmaceutical composition has a half life of at least 48, 72, 96, or 120 hours.

Also ed herein is a pharmaceutical ition comprising MGBG together with at least one oral pharmaceutically acceptable ent, which yields a therapeutically effective systemic plasma MGBG level when orally administered to a subject, which does not have substantially dose-limiting side effects. In n embodiments, said side effects are gastrointestinal. In further embodiments, said gastrointestinal side effects are chosen from nausea, vomiting, diarrhea, abdominal pain, oral mucositis, oral ulceration, pharyngitis, stomatitis, gastrointestinal perforations, gastrointestinal ulcers, intestinal obstructions, and gastrointestinal bleeds. In further embodiments, said gastrointestinal side effects are chosen from inhibition of gastrointestinal mucosal proliferation, inhibition of ion of developing epithelial lumen cells, and inhibition of differentiation of stem or progenitor cells into epithelial lumen cells. In certain embodiments, said side effects are chosen from thrombocytopenia, leukopenia, phlebitis, laryngitis, cellulitis, dermatitis, and hypoglycemia.

Also provided herein is a low—dose oral pharmaceutical composition for c delivery, comprising a therapeutically ive amount of MGBG and at least one pharmaceutically acceptable excipient, which does not have substantial gastrointestinal side effects. In certain embodiments, the low-dose oral pharmaceutical composition for chronic delivery, comprising a therapeutically effective amount of MGBG and at least one pharmaceutically able ent, which does not have substantial gastrointestinal side 2014/010714 effects, yields a therapeutically effective plasma level of MGBG for at least a 24 hour period in the subject with once-daily dosing.

In certain embodiments, the pharmaceutical composition is formulated as a tablet or capsule. For example, in certain ments, the pharmaceutical composition ses: 01-50% of a polyamine analog or a polyamine biosynthesis inhibitor; 0.1-99.9% of a ; 0-10% of a egrant; 0-5% of a lubricant; and, 0-5% of a glidant.

In certain embodiments, the pharmaceutical composition comprises: 01-50% of MGBG; 0.l-99.9% of a filler; 0-10% of a disintegrant; 0-5% of a lubricant; and, 0-5% of a glidant.

In further embodiments, said filler is chosen from a sugar, a starch, a cellulose, and a poloxamer; said disintegrant is chosen from povidone and crospovidone; said lubricant is magnesium stearate; and said glidant is silicon dioxide.

In further embodiments, said filler is chosen from lactose and microcrystalline cellulose; said egrant is chosen from povidone and crospovidone; said lubricant is magnesium te; and said glidant is silicon dioxide.

In certain embodiments, the pharmaceutical ition comprises: -300 mg of a polyamine analog or a polyamine biosynthesis inhibitor, making up 2- 50% of the tablet content or capsule fill content; 0-10% of a disintegrant; 0-5% of a lubricant; 0-5% of a glidant; and -98% of a filler.

In certain embodiments, the pharmaceutical ition comprises: -300 mg of MGBG, making up 2-50% of the tablet content or capsule fill content; 0-10% of a disintegrant; 0-5% of a lubricant; 0-5% of a glidant; and -98% of a filler.

In r embodiments, the pharmaceutical composition comprises 01-10% of a binder; 0-5% of a surfactant; 0-10% of an intergranular disintegrant; and 0-10% of an extragranular egrant.

In further embodiments, the pharmaceutical ition may additionally comprise 0-10% of a ; 0-5% of a surfactant; 0-10% of an intergranular disintegrant; and 0-10% of an extragranular disintegrant.

In further embodiments, said binder is chosen from copolyvidone, hydroxypropyl-cellulose, hydroxypropylmethylcellulose, and povidone; said surfactant is chosen from polyoxyethylene (20) sorbitan monooleate, a poloxamer, and sodium lauryl sulfate; said intergranular disintegrant is chosen from rmellose sodium, sodium starch ate, and crospovidone; and said extragranular disintegrant is chosen from croscarmellose sodium, sodium starch glyconate, and crospovidone.

Also provided herein is a method of treating or delaying the onset or development of a condition in a t in need thereof sing the administration of an oral pharmaceutical composition comprising MGBG and at least one pharmaceutically acceptable excipient. In certain embodiments, the oral pharmaceutical composition is delivered in a therapeutically effective amount. In certain embodiments, said oral pharmaceutical composition has an oral bioavailability of at least 30%. In certain embodiments, said oral pharmaceutical composition does not have substantially dose-limiting side effects. In certain embodiments, the plasma level of MGBG is at least 75% of the peak plasma concentration for 4 or more hours. In further ments, said oral pharmaceutical composition yields a therapeutically effective systemic plasma MGBG level for at least a 12-hour period when orally administered to a subject.

In certain embodiments, said condition is chosen from a proliferative disorder, an inflammatory disease, and an mune disease, a neuropathy, and a neurodegenerative disease. In certain embodiments, said condition is chosen from rheumatoid arthritis, osteoarthritis, multiple sis, amyotrophic lateral sclerosis, HIV neuropathy, and HIV associated dementia.

In certain ments, said proliferative disorder is chosen from a cancer, psoriasis, tic arthritis and atopic dermatitis. In certain embodiments, the neuropathy is chosen from peripheral neuropathy, diabetic neuropathy, entrapment neuropathy (carpel tunnel me), postherpetic neuralgia (PHN), herapy-induced athy, and HIV neuropathy.

In certain embodiments, the condition is chosen from a proliferative disorder, rheumatoid arthritis, osteoarthritis, multiple sclerosis, and amyotrophic lateral sclerosis,. In n embodiments, the proliferative disorder could, for example, be chosen from a cancer, psoriasis, psoriatic arthritis, and atopic dermatitis.

Also provided is an oral ceutical ition, comprising a polyamine analog or polyamine biosynthesis inhibitor together with at least one oral pharmaceutically acceptable excipient, which yields a therapeutically effective systemic plasma level of the polyamine analog or polyamine biosynthesis inhibitor for the treatment of pain when orally administered to a subject. Also provided is an oral pharmaceutical composition, comprising MGBG together with at least one oral pharmaceutically acceptable excipient, which yields a therapeutically ive systemic plasma level of MGBG for the treatment of pain when orally administered to a subject.

Also provided herein is a method of treatment of pain in a subject in need thereof comprising the administration of a polyamine analog or a polyamine biosynthesis inhibitor, or a salt or protected derivative thereof. Also provided herein is a method of treatment of pain in a subject in need thereof comprising the administration of MGB G. In certain embodiments, the MGBG is administered in a therapeutically effective amount. Further provided is a method of treatment of pain in a subject in need f comprising the administration of a therapeutically effective amount of a pharmaceutical composition comprising MGBG and at least one pharmaceutically able excipient.

In certain ments, the pain is chosen from inflammatory pain, pain due to nerve injury, chronic pain, intractable cancer pain, complex regional pain syndrome, athic pain, surgical or post—surgical pain, dental pain, pain resulting from dermal injury, lower back pain, headaches, migraine, tactile allodynia, and hyperalgesia. In certain ments, the pain is chronic. In other embodiments, the pain is acute. In certain embodiments, the pain is inflammatory pain.

In certain embodiments, the administration of MGBG or its pharmaceutical composition is oral. In other embodiments, the administration is intravenous.

In certain embodiments, the administration is a combination of oral and intravenous. In certain embodiments, the first administration is oral and the second IV; in others the first is IV and the second oral; in either case, additional oral or IV dosing may follow. In certain embodiments, the pain is surgical or urgical pain. For example, in certain ments, the pre-surgical administration is oral and the peri-surgical administration is IV; in others the pre-surgical administration is IV, the pre—surgical administration is also IV, and the post-surgical administration is oral. In either case, additional oral or IV dosing may . In certain embodiments, the pre-, peri-, and post- al administration is IV.

Also provided herein is a method of treatment of a condition in a subject in need thereof sing the administration of an oral pharmaceutical composition sing MGBG and at least one pharrnaceutically acceptable excipient; and r therapeutic agent.

In certain embodiments, the MGBG is delivered in a therapeutically effective amount. In other embodiments, the MGBG is delivered in a subtherapeutic . In certain embodiments, the other therapeutic agent is delivered in a therapeutically effective amount. In other embodiments, the other therapeutic agent is delivered in a subtherapeutic amount. In certain embodiments, the MGBG and the other therapeutic agent are delivered together in amounts which would individually be subtherapeutic but which together are eutically effective. In other embodiments, the MGBG and the other therapeutic agent are delivered er in amounts which are individually therapeutically ive.

Additionally provided herein is a method of treating a condition. The method comprises administering to a subject in need of such treatment an effective amount of MGBG, a salt of MGBG, a protected derivative of MGBG, or a polyamine analog or polyamine biosynthesis inhibitor or a salt, a protected derivative, or a stereoisomer thereof, n the condition is chosen from Crohn‘s disease, Parkinson's disease, inflammatory bowel disorder, multiple sclerosis (MS), amyotrophic lateral sclerosis (ALS), hepatitis, HBV, WO 10154 HCV, nephritis, cerebritis, glomerulonephritis, rheumatoid arthritis, type 2 diabetes, cardiac fibrosis and angiotensin type II ated hypertension, osteoporosis, a mast cell produced IgE mediated hypersensitivity immune reaction, peripheral y neuropathy associated with HIV infection or diabetes mellitus, asthma, autism, dermatomyositis, frailty, obesity, primary biliary cirrhosis, primary sclerosing cholangitis, post-radiation syndrome, tic arthritis, dosis, scleroderma with or without ary is, a kidney related autoimmune condition, diabetic pathy, a diabetic vascular complication, and a lymphoproliferation related autoimmune condition.

Additionally provided herein is a method of decreasing differentiation of macrophages from monocytes, sing contacting a monocyte with an effective amount of an agent that inhibits S-adenosyl methionine decarboxylase or inhibits polyamine biosynthesis in the monocyte. In certain embodiments the agent is MGBG, or a salt or protected derivative thereof.

In one embodiment, the agent is e of inhibiting SAMDC or any pathway ning AMD 1, e.g., any entity upstream or downstream of a pathway containing SAMDC, especially any pathway ning SAMDC and associated with adenosine production. In another embodiment the agent is capable of inhibiting polyamine biosynthesis or any pathway ed in polyamine biosynthesis. In general, a pathway containing SAMDC or adenosine is understood to refer to a pathway in which either SAMDC or adenosine is involved, including, for example, as a substrate, catalyst, t or by-product.

The agent can be any kind of known or later discovered agent that can inhibit the activity of the enzyme S-adenosyl methionine decarboxylase, can inhibit polyamine biosynthesis in, for example, a cell. In one embodiment, the agent is a chemical agent, including, but not limited to, organic molecules and salts, protected derivatives and stereoisomers thereof, inorganic molecules or various ionic or tal entities.

Compounds for use in the methods and compositions disclosed herein include polyamine s and polyamine biosynthesis inhibitors, as well as salts, prodrugs, solvates, anhydrous forms, protected derivatives, structural isomers, stereoisomers, amino acid conjugates, and porphyrin conjugates thereof. Any ine analog is suitable for use in the methods of the present invention.

Exemplary polyamine analogs used in the methods of the invention include compounds of the structural formulas l, 2, 3, 4, 5, 6, and 7 and the ponding stereoisomers, salts, and protected derivatives thereof.

Formula 1 has the structure 2014/010714 wherein R1, R2, R4, R6 and R7 are independently chosen from hydrogen, alkyl and aryl; and R3 and R5, are alkyl groups.

Formula 2 has the structure /N\ /N\ /N\ /N\ R1 R3 R5 R7 R9 wherein R1, R2, R4, R6, R8, and R9 are independently chosen from hydrogen, alkyl and aryl; R3, R5 and R7 are alkyl groups.

Formula 3 has the structure N\ /N\ /N\ /N\ /N\ R1/ R3 R5 R7 R9 R11 wherein R1, R2, R4, R6, R10 and R11 are independently chosen from hydrogen, alkyl and aryl; R3, R5, R7 and R9 are alkyl .

Formula 4 has the structure N N N N /\/\/\/\ R1 R2 R3 R4 R5 wherein R1 and R5 are independently chosen from methyl, ethyl, yl, and isopropyl; R2, R3, and R4 are independently chosen from C1-C6 alkyl, C2-C6 alkenyl, C3—C6 cycloalkyl, C1-C6 alkyl-C3-C6 cycloalkyl- C1-C6 alkyl, C3-C10 aryl, and C1-C6 alkyl—C3-C10 aryl- C1-C6 alkyl; and R6, R7, R8 and R9 are independently chosen from hydrogen, methyl, and ethyl; Formula 5 has the structure wherein R1 and R6 are independently chosen from methyl, ethyl, n-propyl, and isopropyl; R2, R3, R4 and R5 are independently chosen from C1—C6 alkyl, C2-C6 alkenyl, C3-C6 cycloalkyl, C1-C6 alkyl- C3-C6 cycloalkyl- C1-C6 alkyl, C3—C10 aryl, and C3—C10 aryl- C1-C6 alkyl; and R7, R8, R9, R10 and R11 are independently chosen from hydrogen, , and ethyl.

In another embodiment, the polyamine analogs are compounds of the structures 2 and 3, wherein R3, R5, R7 and R9 are independently (CH2)x ; X is an integer from 2 to 6; and R4, R6 and R8 are hydrogen atoms.

In yet another embodiment, the ine analogs are compounds of the structures 2 and 3, wherein R3, R5, R7 and R9 are independently (CH2)X groups; x is an integer from 2 to 6; R4, R6 and R8 are hydrogen atoms; R] and R10 are alkyl groups; and R2 and R11 are en atoms.

In yet another embodiment, the polyamine analogs are compounds of the structures 2 and 3, wherein R3, R5, R7 and R9 are independently (CH2)x groups; X is an integer from 2 to 6; R4, R6 and R8 are hydrogen atoms; R1 and R10 are alkyl groups; R2 and R11 are hydrogen atoms; and the polyamine analogs have a molecular weight less than 500.

Further embodiments of compounds of the structure 4 include those wherein R6, R7, R8 and R9 are hydrogen.

In other embodiments, R1 and R5 are ethyl.

In yet r embodiments, R6, R7, R8 and R9 are hydrogen; and R1 and R5 are ethyl.

In yet further embodiments, R2 and R4 are independently chosen from C1-C6 alkyl; and R3 is chosen from C1-C6 alkyl, C2-C6 l, C3-C6 cycloalkyl, C1—C6 alkyl— C3-C6 cycloalkyl— C1-C6 alkyl, C3—C10 aryl, and C1—C6 alkyl- C3-C10 aryl- C1-C6 alkyl.

Additional polyamine analogs useful in the present invention include compounds of the formula 6, and the corresponding stereoisomers, salts, and protected derivatives thereof: T8 $9 T10 T11 R/N\ /N\ /R1 R2 R3 4\Rs/N\ /N\R6 R7 wherein R4 is chosen from C2-C6 n-alkenyl, C3-C6 cycloalkyl, C3-C6 lkenyl, and C3-C6 aryl; R3 and R5 are ndently chosen from a single bond, C1-C6 alkyl, and C1—C6 R2 and R6 are independently chosen from C1—C6 alkyl, C1-C6 alkenyl, C3-C6 cycloalkyl, C3-C6 cycloalkenyl, and C3-C6 aryl; R1 and R7 are independently chosen from hydrogen, C1-C6 alkyl, and C2-C6 alkenyl; R3, R9 and R11 are hydrogen.

, R10, In certain embodiments of the compounds of a 6, R1 and R7 are independently chosen from C1-C6 alkyl and C2-C6 alkenyl.

Additional polyamine analogs useful in the present invention include compounds of the formula 7, and the corresponding stereoisomers, salts, and protected derivatives thereof: R8 R9 R10 R11 I I | | N N R N N /\/\/ 4‘R5/\/\ R1 R2 R3 R5 R7 wherein R4 is chosen from C1—C6 n-alkyl and C1-C6 branched alkyl; R3 and R5 are independently chosen from a single bond or C1-C6 alkyl; R2 and R6 are independently chosen from C1-C6 alkyl, C1-C6 l, C3-C6 lkyl, C3-C6 cycloalkenyl, or C3-C6 aryl; R1 and R7 are independently chosen from H, C1-C6 alkyl, or C2-C6 alkenyl; and R3, R9, R10, and R11 are hydrogen.

In certain embodiments of the compounds of formula 7 R2 and R7 are independently chosen from C1-C6 alkyl or C2-C6 alkenyl; R4 is chosen from C1-C6 saturated l and C1-C6 saturated branched alkyl; and R3 and R5 are independently chosen from a single bond and C1-C6 saturated n-alkyl. ing to another embodiment of the present invention, the agent is a chemical moiety that inhibits the activity of S—adenosyl methionine decarboxylase, inhibits polyamine biosynthesis, and/or increases the activity of adenosine.

Examples of such moieties include, but are not limited to, those listed in Table l.

Irrespective of the form of the moiety listed in Table 1, it is tood that it includes, as applicable, a salt, protected derivative, and stereoisomer thereof.

Table 1.

Pub Chem Compound al Name (Not IUPAC) Decarboxylated s-adenosy1methylthiopropylamine 535 l 154 Mitoguazone or yl 1 oxalb'1s(guany1h dy ) 9561662 "MGBG" EGBG Ethylglyoxal bis(guanylhydrazone) 2354 Berenil Diminazene or Diminazene aceturate 4735 4- [5-(4—carbamimidoylphenoxy)pentoxy] benzenecarboximidamide Pentamidine 5'-(Dimethy1sulphino)-5‘-deoxyadenosine S-adneosylmethylthiobutyrate S-adenosy1—S-methyl-L-cysteine S-(5'—Deoxy-5'-adenosy1) methylthioethylhydroxylamine EMGBG Ethylmethylglyoxal bis(guanylhydrazone) DEGBG Diethylglyoxal bis(guanylhydrazone) 1 6-((2-carbamimidoylh drazono)methy yl) CGP-33'829 5479208 picolinimidamide CGP-36'958 CGP-39'937 2,2'-bipyridine-6,6'-bis(carboximidamide) CGP-48664 or 4-amidinoindanone 2‘-amidinohydrazone 548681 1 CGP48664Aor SAM 364A AbeAdo 5'-[[(Z)aminobuteny1] methylamino]—5'— 6436013 0rMDL-7381 1 deoxyadenosine '—deoxy-5‘-[N—methy1-N—[2- MAOEA 3081018 (aminooxy)ethy1]amino]aden0sine '—deoxy-5'—[N—methyl-N—(3- MHZPA 122092 hydrazinopropy1)amin0]adenosine '-deoxy-5'—[(2—hydrazinoethy1)— MHZEA methylamin0]adenosine S-(5‘-deoxy- 5'—adenosy1)amm0niO-4— AdoMac 4 (methylsulfonio)-2cyc10pentene deoxy- 5'—adenosy1)amin0xy-4— AdoMac (methylsulfonio)-2—cyclopentene APA 1-Amin00xy-3—amin0pr0pane 65020 AOE-PU N- [2-amin00xyethy1]—1,4-diamin0butane 1-amin00xyN-[3-amin0Pr0Py1]-amin0Pr0Pane AP-APA 1 ,1 1-bis(ethy1)n0rspermine BES 1,8-bis( ethy1)spermidine BES 1,12-bis(ethy1)spermine DESPM N1 ,N12-diethy1spermine BE3-3 1,1 1-bis(ethylamin0)-4,8-diazaundecan BE4-4 1 ,14-bis(ethy1amin0)-5,10-diazatetradecane DEHOP or Diethylhomospermine, N1 ,N14— DEHSPM diethylhomospermine DENOP diethyl—norspermine BE44 1 ,19-bis(ethy1amin0)-5,10,15-triaza-nonadecane l-N'—(2-(3‘-ethylamin0-pr0py1amin0 SL1 1037 methyl)-Cis-Cyclopr0py1methy1)-pr0pane 1,3- diamine tetrahydrochloride N-ethy1-N'-(2-(3'—eth lamino-y pr0pylamino SL11038 methyl)-trans—cyclobuty1methy1)-pr0pane 1,3- diamine tetrahydrochloride N-ethyl-N'-(2—(3‘-ethylamino—propylamino SL1 1044 methyl)-transcyclopropylmethyl)-propane 1,3- diamine ydrochloride SL1 1047 or N,N'-bis(3-ethylaminopropyl)-cis-but-2—ene-1,4— SL47 diaminetetrahydrochloride N,N'-(cyclopropane-1,2— SL11093 or diylbis(methylene))bis(N4—ethylbutane-1,4— SL93 In yet another embodiment, the agent is a compound chosen from MGBG, MDL73811, CGP48664, Berenil, Pentamidine, SL47, and SL93, or a combination of two or more thereof. In yet another embodiment, the agent is MGBG, SL4";I or SL93. In still another embodiment, two or more agents are used. The two or more agents can be used either sequentially or simultaneously.

MGBG is 1,1’[methylethanediylidene]dinitrilodiguanidine and is also known as methylglyoxal bis(guanylhydrazone), methyl-GAG, Me-G, and mitoguazone. As used herein, MGBG includes the free base and salts thereof. It is commonly, but not necessarily, used as a dihydrochloride. MGBG may be present as any one of the following isomers, or a tautomer and/or a syn/anti isomer thereof, e of one or more thereof: ,N \,N NH \ N \/N NH2 H2N N Y\N \n/ 2 H2N N \ N \W/ H NH NHz , , NH )sz NH2 H2N \N H HZNANJH H H2N)\N \’N NH N\ \,N NH2 N\ NH2 \/N \/ \ \ N \n/ 2 Y\N \n/ N NH NH NH2 , , , H2N \ N NH NH2 NH2 ’Nj/§N H2NJ\\N’N\ “)1 H2NJ\\N’N\ \N H Hl\|l\n/NH2 NYNHZ HN\n/NH2 NH NH2 NH , , , NH NH2 NH2 HZNJJWNH H2NJRI}J H2NJ§N In certain embodiments, MGBG may be present one of the ing isomers, or a tautomer and/or a syn/anti isomer thereof, e of one or more thereof: H HZNJLNH HZNAN/NVN’NYNHZ H H NH NWANNTNHZNH , , NH HZNANH In certain embodiments, compounds have a structure chosen from Formulas 8a- F52 F53 $4 F55 RrNME“Mn”MDNR, (8a) H H H N N H R1/ My MW Mx My M2 \Re (8b) R1 R7 R7 R6 N N LN—(CHfln—NL) (8C) R1 — R6 are chosen from hydrogen, alkyl and aralkyl having from 1 to 12 carbon atoms, provided that, in formula (8a), R1, and R6 are not hydrogen; R7 chosen from hydrogen, alkyl, aryl and aralkyl having from 1 to 12 carbon atoms; m, n, are each independently an integer from 3 to 6, inclusive; and V, w, X, y, and z are each independently an integer from 3 to 10, inclusive.

Additional disclosure may be found in WO98/ 10766, the disclosure of which is incorporated by reference as if written herein in its entirety, for e on pp. 3-4.

In certain embodiments, compounds have a structure of Formula 9a: E-NH-B-A-B-NH-B-A-B-NH-B-A-B-NH-B-A-B-NH-E wherein A is independently selected from the group consisting of C1-C6 alkyl, C2-C6 alkenyl, C2-C6 alkynyl, C3-C6 cycloalkyl, C3-C6 aryl, and C3-C6 cycloalkenyl; B is independently selected from the group consisting of: a single bond, C1-C6 alkyl, and C2-C6 alkenyl; and E is independently selected from the group consisting of hydrogen, C1-C6 alkyl, C2-C6 alkenyl, C2-C6 alkynyl, C3-C6 cycloalkyl, C3-C6 aryl, and C3-C6 cycloalkenyl; with the o that either at least one A moiety is selected from the group consisting of C2-C6 l, C2-C6 alkynyl, C3—C6 cycloalkyl, C3-C6 aryl, and C3-C6 cycloalkenyl, or at least one B moiety is selected from the group consisting of C2-C6 l; and all salts, es, solvates, and stereoisomers thereof.

In another embodiment, the mationally restricted polyamine analog is selected from among the group of compounds of the formula 9b: E—NH-B—A-B-NH-B-A-B—NH-B-A-B-NH(—B-A-B—NH)X-E wherein: A is independently selected from the group consisting of C1-C6 alkyl, C2—C6 alkenyl, C2-C6 alkynyl, C3-C6 cycloalkyl, C3-C6 aryl, and C3-C6 cycloalkenyl; B is independently selected from the group consisting of: a single bond, C1-C6 alkyl, and C2-C6 l; and E is independently selected from the group consisting of hydrogen, C1-C6 alkyl, C2-C6 l, C2-C6 l, C3-C6 cycloalkyl, C3-C6 aryl, and C3-C6 lkenyl; X is an integer from 2 to 16; with the proviso that either at least one A moiety is selected from the group consisting of C2-C6 alkenyl, C2-C6 alkynyl, C3—C6 cycloalkyl, C3-C6 aryl, and C3-C6 cycloalkenyl, or at least one B moiety is selected from the group consisting of C2-C6 alkenyl; and all salts, hydrates, solvates, and stereoisomers thereof.

In another embodiment, X is 4,6, 8, or 10.

In another embodiment, X is 4. In another embodiment, X is 6.

In another embodiment, X is 8.

In another embodiment, X is 10.

In another embodiment, the conformationally restricted ine analog is selected from among the group of compounds of the formula 9c: -A-B-NH-B-A-B-NH-B-A-B-NH(-B-A-B-NH)X-E wherein: A is independently selected from the group consisting of C1-C6 alkyl, C2-C6 alkenyl, C2-C6 alkynyl, C3-C6 cycloalkyl, C3-C6 aryl, and C3-C6 cycloalkenyl; B is independently selected from the group consisting of: a single bond, C1-C6 alkyl, and C2-C6 alkenyl; and E is independently selected from the group ting of C1-C6 alkyl, C1-C6 alkanol, C3-C6 cycloalkanol, and C3-C6 hydroxyaryl, with the proviso that at least one B moiety be selected from the group consisting of C1-C6 alkanol, C3-C6 cycloalkanol, and C3-C6 hydroxyaryl; and X is an integer from 0 to 16; and all salts, hydrates, solvates, and stereoisomers thereof.

In another embodiment, the conformationally restricted polyamine analog is selected from among the group of compounds of the formula 9d: E-NH—D-NH—B-A-B—NH—D-NH-E wherein A is ed from the group consisting of C2-C6 alkene and C3-C6 cycloalkyl, cycloalkenyl, and aryl; B is independently selected from the group consisting of a single bond and C1-C6 alkyl and alkenyl; D is independently selected from the group consisting of C1-C6 alkyl and alkenyl, and C3-C6 cycloalkyl, cycloalkenyl, and aryl; E is independently selected from the group consisting of hydrogen, C1-C6 alkyl and l; and all salts, hydrates, solvates, and isomers thereof.

In another embodiment, the conformationally restricted polyamine analog is selected from yclic ines of the formula 9e: Jk A 1 M N/ NY A Y ' A A 2 R N’ 3‘N k Y Y wherein A1, each A2 (if present), and A3 are ndently selected from C1-C8 alkyl; each Y is independently selected from hydrogen or C1-C4 alkyl; M is ed from C1-C4 alkyl; k is 0, 1,2, or 3; and R is selected from C1-C32 alkyl; and all salts, hydrates, solvates, and stereoisomers thereof.

In additional embodiments, the Y group is hydrogen or —CH3.

In another embodiment, A1, each A2 (if present), and A3 are independently selected from C2-C4 alkyl.

In yet another embodiment, M is -CH2-.

In another embodiment, the conformationally cted polyamine analog is selected from macrocyclic ine analogs of the formula 9f: RAv/AsvMAS‘LAFY}A2 k wherein A1, each A2 (if present), and A3 are independently selected from C1-C8 alkyl; A4 is selected from C1-C3 alkyl or null; X is selected from - hydrogen, —2, -CN, -NH2, -C(=O)- C1-Cg-alkyl, or -NHZ, with the proviso that when A4 is null, X is hydrogen, -C(=O)- C1—Cg-alkyl, or -Z; Z is selected from the group consisting of an amino protecting group, an amino capping group, an amino acid, and a peptide; each Y is ndently selected from hydrogen or C1-C4 alkyl; M is selected from C1-C4 alkyl; k is 0, 1,2, or 3; and R is selected from C1—C32 alkyl; and all salts, hydrates, solvates, and stereoisomers thereof.

In certain embodiments, A4 is null.

In other embodiments, X is -Z, and -Z is hydrogen.

In other embodiments, X is -Z, and -Z is 4-morpholinocarbonyl.

In other embodiments, X is -Z and -Z is acetyl.

In other ments, X is -Z and -Z is t-Boc or Fmoc.

In other embodiments, Y is -CH3.

In other embodiments, M is -CH2-.

In still further embodiments, k is 1.

In further embodiments, A, and A3 are —CH2CH2CH2-.

In still further embodiments, - CHZCHZCHZCH2—.

In still further embodiments, R is C13H27.

In yet further embodiments, one or more of the ic limitations on A4, X, Z, Y, M, k, A1, A3, and R are combined.

In further ments of macrocyclic polyamine analog compounds, A4 lS C1-C8 alkyl, X is —NHZ; and Z is selected from one of the 20 genetically encoded amino acids (alanine, cysteine, aspartic acid, glutamic acid, phenylalanine, glycine, histidine, isoleucine, lysine, methionine, asparagine, proline, glutamine, arginine, serine, threonine, valine, tryptophan, ne), a peptide of the formula acetyl-SKLQL-, a peptide of the formula acetyl- SKLQ—I3-alanine-, or a peptide of the formula acetyl-SKLQ-.

In these cases, where Z is an amino acid or peptide, the therapeutic agent to be used is a polyamine-amino acid conjugate or polyamine-peptide conjugate.

In one embodiment, the only conformational restriction of the polyamine analog is due to a carbon-carbon double bond (an ethenyl group, C=C) in the le.

In r embodiment, the only conformational restriction of the ine analog is due to a cycloalkyl group, such as a cyclopropyl group, in the molecule.

W0 2014l110154 nds include, but are not limited to: H H /\N/\/\/N\/\/\N/\/\/N\/\/\N/\ .5HCI H H H H H HOWNWNNNWNNNA’5HC' H H H H H HO\/\N/\/\/N\/\/\N/\/\/N\/\/\N/\ H H H /\/\u/\/\ 02HC| ”HZ K WMNWNACHH H ANWNVO7HC| AN/WQNV’10HCI Amw1x/ W0 2014l110154 H H H H \/N\/\/N\/—\/N\/\/N\/_ ‘4HCI H H \/N\/\/N\/\/\N/\/\N/\ ‘4HCI H H H H H H H H NNNMNW H H H H HOWMWNRNWMQ_ H H AHWNRNWNQ ¢4HC|_ W0 2014l110154 2014/010714 H H H H H VNflNflNflNflNV .4HCI 2]: H H H \/ \/\/\N/\/\/N\/=\/N\/=\/N\/ ¢4HC| H H H H \/N\/=\/N\/\/\N/\/\/N\/=\/N\/ ’4HCI H H W0 10154 W0 2014l110154 \/N\/\/ \/\/N\/ H H AMWN N\/\/\N/\ 04HC| H H D. H H /N\5 3/N\/\/N\/ W0 10154 HN HN HN HN ‘4HCI N\/\/\N/\ ’4HCI HN HN .4HC| NH NH HN HN NH HN\/ HN HN V H2N NH2 NH NH HN HN NH NH WO 10154 /\/\ onal disclosure may be found in WO2007I040535, the disclosure of which is incorporated by reference as if written herein in its entirety.

Additional analogs and tives include those encompassed by the following formula 10a: R-X-polyamine wherein R is selected from H or from the group of a straight or branched Cl-50 saturated or unsaturated aliphatic, carboxyalkyl, carbalkoxyalkyl, or alkoxy; a C1-8 alicyclic; a single or ing aryl tuted tic; an aliphatic-substituted single or multiring aromatic; a single or multiring heterocyclic; a single or multiring heterocyclic 25 aliphatic; a Cl-10 alkyl; an aryl sulfonyl; or cyano; X may be -CO-, —SOZ, or —CH2- and "polyamine" may be any naturally occurring, such as putrescine, spermine or sperrnidine, or synthetically produced polyamine.

Preferably, R is at least about C5, at least about C10, at least about C11, at least about C12, at least about C13, at least about C14, at least about C15, at least about C16, at least about C17, at least about C18, at least about C19, at least about C20, or at least about C22.

The linkage between X and the polyamine may be direct, wherein there are no atoms between X and the nitrogen of the amine group of the polyamine, or indirect, where there may be one or more atoms between X and the nitrogen of the amine group of the polyamine. The linkage. between X and the polyamine may occur via any amino group within the polyamine, although a primary amino group is used in preferred embodiments of the invention.

In red embodiments of the invention where the linkage between X and the polyamine is indirect, the intervening one or more atoms are ably those of an amino acid or a tive thereof. In particularly preferred embodiments of this type, the intervening one or more atoms are those of lysine, aspartic acid, glutamic acid, omithine, or aminobutyric acid. Preferred compounds of this type may be represented as in Formula 10b: R-X-L-polyamine wherein R is a ht or branched C10-50 saturated or unsaturated aliphatic, carboxyalkyl, carbalkoxyalkyl, or alkoxy; a C1-8 alicyclic; a single or multiring aryl substituted or 2014/010714 unsubstituted aliphatic; an aliphatic-substituted or unsubstituted single or multiring aromatic; a single or multiring heterocyclic; a single or multiring heterocyclic aliphatic; an aryl sulfonyl; X is -CO-, —SOz-, or -CHz-; and L is a nt bond or a naturally occurring amino acid, ornithine, 2,4- diaminobutyric acid, or derivatives thereof. [03 50] The analogs and derivatives of the invention, may be optionally further substituted at one or more other positions of the polyamine. These include, but are not limited to, internal nitrogen and/or internal carbon atoms. In one aspect of the invention, preferred substituents are structures that increase polyamine transport inhibition, binding ty or ise enhance the irreversibility of binding of the compound to a polyamine binding molecule, such as the polyamine transporter, an enzyme or DNA. Such additional substituents include the aziridine group and various other aliphatic, aromatic, mixed aliphatic-aromatic, or heterocyclic multi-ring structures. Reactive moieties which, like ine, bind covalently to a polyamine transporter or another polyamine binding molecule, are also within the scope of this invention. Examples of reactive groups that react with nucleophiles to form nt bonds include chloro-, bromo- and iodo-acetamides, sulfonylfluorides, esters, nitrogen mustards, etc. Such reactive es are used for affinity labeling in a diagnostic or research context, and may contribute to cological activity in inhibiting polyamine transport or polyamine synthesis. The reactive group can be a reactive photoaffinity group such as an azido or henone group. Chemical agents for photoaffinity labeling are nown in the art (Flemming, S.A., Tetrahedron 1995,51, 12479-12520).

A preferred aspect of the invention relates to a ine analog or derivative that is a highly specific polyamine transport inhibitor with pharmaceutical utility as an anticancer herapeutic. One class of a polyamine analog or derivative of the invention that binds to a polyamine-binding site of a le and/or inhibits polyamine transport, is described by the following formula 10c: (O)n HN XR1 Rz‘xlMET“MaNMNMON” (0)n wherein a, b, and 0 independently range from 1 to 10; d and e independently range from 0 to 30; each X is independently either a carbon (C) or sulfur (S) atom, and R1 and R2 are as described below, or each of R1X(O)n- and of n- are independently replaced by H; and * denotes a chiral carbon position; and with the provisos that ifX is C, then n is 1; ifX is S, then n is 2; and if X is C, then the X(O) group may be CH2 such that n is o.

In the above formula, R1 and R2 are independently selected from H or from the group of a ht or branched Cl—50 saturated or unsaturated aliphatic, carboxyalkyl, carbalkoxyalkyl, or alkoxy; a Cl—8 alicyclic; a single or multiring aryl substituted aliphatic; an aliphatic-substituted single or multiring aromatic; a single or multiring aromatic or saturated heterocyclic; a single or ing heterocyclic aliphatic; a Cl-10 alkyl; an aryl sulfonyl; or cyano.

Examples of heterocyclic rings as used herein include, but are not limited to, pyrrole, furan, ene, imidazole, oxazole, thiazole, pyrazole, 3-pyrroline, pyrrolidine, pyridine, pyrimidine, , quinoline, isoquinoline, and carbazole. [03 54] All of the above described aliphatic, carboxyalkyl, carbalkoxyalkyl, alkoxy, 30 alicyclic, aryl, aromatic, and heterocyclic moieties may, of course, also be optionally substituted with 1—3 substituents independently ed from halo (fluoro, chloro, bromo or iodo), lower alkyl (l-6C) and lower alkoxy .

As used herein, carboxyalkyl refers to the tuent -R‘-COOH wherein R' is alkylene; and koxyalkyl refers to -R'-COOR wherein R' and R are alkylene and alkyl respectively. In preferred embodiments, alkyl refers to a saturated straight- or branched-chain hydrocarbyl radical of 1-6 carbon atoms such as methyl, ethyl, n-propyl, isopropyl, n-butyl, t- butyl, n—pentyl, 2—methylpentyl, n-hexyl, and so forth. Alkylene is the same as alkyl except that the group is divalent. Aryl or alkyl sulfonyl moieties have the formula SOZR and alkoxy moieties have the formula -O-R, wherein R is alkyl, as defined above, or is aryl wherein aryl is phenyl, optionally tuted with 1-3 substituents independently selected from halo (fluoro, chloro, bromo or iodo), lower alkyl (l-6C) and lower alkoxy (l-6C).

A preferred group of compounds encompassed by the above is where d is 4 and e is 0.

An additional class of a polyamine analog or derivative of the ion that binds to a polyamine-binding site of a molecule and/or inhibits polyamine ort, is described by the following formula 10d: HN R1 H ( a H H H Raj/N e* MCNHZ R4 0 wherein a, b, and C independently range from 1 to 10; d and e independently range from 0 to 30; R1 and R2 are defined as above for formula 8c and R3 and R4 are independently ed from organic substituents including -CH3 and as defined above for R1 and R2 in formula 8c above. This grouping of analogs is produced by reductive amination of the free amino precursor with a ketone.

In one preferred embodiment of the invention, R1 and R2 are cal and as described for formula 8c. Positions R3 and R4 may also be identical, and all of R1 through R4 may also be identical. Additionally, each of positions R1, R2, R3, and R4 in a 8d may also be independently H.

In an additional aspect of the invention the proximal and/or the distal amino group relative to the polyamine (such as spermine) can be di-alkylated to form tertiary amines.

These materials can be synthesized by ive amination with a large excess of the carbonyl component. Additionally, these materials may be produced by a conjugate on of the amine precursor to an (LB-unsaturated carbonyl or 0t,[3-unsaturated nitrile.

Each of R1, R2, R3, and R4 can be independently varied and are as defined as above for formula III. Each of R1, R2, R3, and R4 may also be independently H. The values of a, b, c, d and e are as described above for formula 8d. This aspect of the invention is depicted in the following a 10e: R1 \ ,R3 R; e* Mawbr’rcz In a further aspect of the invention, compounds which lack the proximal or distal amino group on the acyl portion of the molecule are also provided. These are represented by formula 10f: z2 H H H NH e* ‘(Vfa ‘(VTb ‘(")’c 2 wherein Z] is NR1R3 and Z2 is selected from - R1, —CHR1R2 or -CR1R2R3 (wherein R1, R2, and R3 are as defined above for formula 8c); or Z2 is NR2R4 and Z1 is selected from -R1, -CHR1R2 or - CR1R2R3 (wherein R1, R2, and R3 are as defined above for formula 8d). Values for a, b, and c independently range from 1 to 10; d and e independently range from 0 to 30. Compounds encompassed by formula V may be prepared by first coupling amino acid derivatives (modified to contain the non-amine ning Z group) to a polyamine followed by appropriate tization of the amine containing Z group. Chemistries for such reactions are known in the art and disclosed .

In preferred embodiments of the invention, positions R1, R2, R3, and R4, of all the formulas set forth above are independently selected from the following, where each of g, h, i, j, and k are ndently selected from 0 to 15: WowE wherein E refers to "entgegen" and Z refers to men".

Compounds include, but are not limited to: W0 10154 W0 10154 W0 2014l110154 2014/010714 TQM NH2 H NNMNH WWNH N\/\/\N/\/\NH H H % NH2 H H NHWN\/\/N\/V\N/\/\NH2H 9 NH2 MNHH Y N NH H NH \‘ MHHMHM NH2 NH H H H O ”WNWNNMMNW Additional disclosure may be found in W02002/053519, the disclosure of which is incorporated by nce as if written herein in its entirety.

Additional analogs and derivatives include synthetic derivatives of al polyamines, wherein a carbon atom of said original polyamine comprises an amide group, said synthetic derivative inhibiting the cellular uptake of a l polyamine by specifically binding a cellular transporter for said natural polyamine.

In certain embodiments, the carbon to which said amido group is located is between two internal nitrogen atoms of said original polyamine.

In certain embodiments, the synthetic derivative ses a dimer of said original polyamine, the monomers of said dimer being linked together by a spacer side chain anchored to the amido group of each monomer.

In certain embodiments, the original ine is selected from the group consisting of putrescine, spermidine and ne.

In certain embodiments, the original polyamine is spermine.

In certain embodiments, said synthetic derivative has the ing general formula 1 la: H H I R‘\| /\ H\2)X/\ /N\/(C@Z/R1 TH (CHQW | u (CH2)y | m C(O) R2 R3 l in which R1 and R11 ndently represent a hydrogen atom or an alkyl group having 1 to 2 carbon atoms, R2, R12, or R3 and R13 independently represent a hydrogen atom or a methyl group, wand z independently represent an integer of 2 or 3, x represents an integer from 0 to n, n represents an integer from 3 to 6, the sum of x and y equals n, and S represents a hydrogen atom or a molecule which cannot be captured by said natural polyamine transporter.

In certain ments, said monomer has the following general formula 11b: H H l R1\| /\ /N\/<CH\2>x/\ /NV<CH\2>Z/R1 TH (CHaw | m (CH2)y | H CK» R2 R3 H'i' in which R1 and R11 independently represent a hydrogen atom or an alkyl group having 1 to 2 carbon atoms, R2, R12, or R3 and R13 independently represent a hydrogen atom or a methyl group, w and 2 independently represent an r of 2 or 3, x represents an integer from 0 to n, n represents an integer from 3 to 6, the sum of X and y equals n, and wherein the spacer side chain comprises a linear hydrocarbon-containing backbone of 3 to 8 atoms.

In certain embodiments, said backbone comprises sulfur, oxygen or nitrogen.

In certain embodiments, w=2, 2:2 X=O and y=3.

In certain ments, w=2, z=2, X=O and y=3.

In n embodiments, w=2, 2:2, X=O and y=4.

Compounds include, but are not limited to: 2N N/Vj/ \/\rH HNK/HN HZN N\/\j\ /\J\ W N NH2 M H NH2 H“MI w K/\NH 2N N\/\J\ «A MW N NH mNVYNN Mwlwx WMj/NVINW WWZHlNMA W0 2014l110154 HZNMMMIWN NH2 M 0 Hszm/fi \/\/NH2 Fm 0 K/NH o \M/\/I \/\/NH2 M O HZNMMMI \/\/NH2 HN O W0 2014l110154 2014/010714 HZNMN N\/\/NH2 fin o o NH2 H2N/V\N WNH2 (\N o 803 s 0 ; NH2 0 N/\/N > H2N/\/\H NWNHZ (\N o 3;” N3 O OH H2N/V\H/\/I \/\/NH2 HN O HN O HN2 \/\/“WI”MNH HZNMN/VI \/\/NH2 HN O HZNMNNI \/\/N NH2 HN O HZNJV\N/\/\/N\/\rNH2H ((EHz) HZNttg Additional disclosure may be found in 7632, the disclosure of which is incorporated by reference as if written herein in its entirety.

Additional analogs and tives include those encompassed by the following formula 12a: RHNMH |\ H /\/ ‘R1 wherein, n can be 0 to 8 and the aminomethyl functionality can be ortho, meta or para substituted, R is hydrogen, —CH3,-CH2CH3, oethyl, 3—aminopropyl, 4—aminobutyl, 5- aminopentyl, ohexyl, 7-aminoheptyl, S-aminooctyl, N-methylaminoethyl, N- methylaminopropyl, N—methyl-4—aminobutyl, N—methyl—S—aminopentanyl, N-methyl aminohexyl, N-methylaminoheptyl, N-methyl-S—aminooctyl, N-ethylaminoethyl, N- ethyl minopropyl, N-ethylaminobutyl, N-ethyl-S-aminopentyl, N-ethylaminohexyl, N-ethyl-7—aminoheptyl or N-ethylaminooctyl and R, is a moiety selected from the group consisting of a hydrogen or a straight or branched CI-20 saturated or unsaturated aliphatic; aliphatic amine but not propylamine when R =H, n=1 and the aminomethyl functionality is para substituted; an alicyclic; single or ring aromatic; single or multi—ring aryl substituted aliphatic; aliphatic-substituted single or multi-ring aromatic; a single or multi-ring cyclic, a single or multi-ring heterocyclic-substituted aliphatic; an aliphatic-substituted aromatic; and halogenated forms thereof.

In certain embodiments, the analogs and derivatives that can be used according to this disclosure can be r modified as described in formula 12b: RHNWN R3 R4 H |\ “ /\/ ‘R1 wherein n can be 0 to 8, R and R1 are described as above, R; can be ndently selected from hydrogen, -CH3,-CH2CH3, and R3 and R4 may be the same or different and are independently selected from hydrogen, or e.

In certain embodiments, compounds that can be used according to this disclosure are described in formula 12c: R3 R4 RHNWN R3 R4 m HVM N R n R2 0 wherein, m and n can be 0 to 7 ndently, but m cannot equal 11 when R1 equals R2 and R3 equals R4, 0 can be 2 to 4, R can be independently selected from H, -CH3,-CH2CH3, R1 and R2 can be independently selected from en, -CH3,-CH2CH3, and R3 and R4 may be the same or different and are independently selected from hydrogen or fluorine.

In certain embodiments, compounds have formula 12d: wherein, R is hydrogen, --CH3,-CH2CH3, m and n can be 0 to 7 independently and o can be 2 to 4, R2 can be independently selected from hydrogen, -CH3,-CH2CH3, and R3 and R4 may be the same or ent and are independently selected from hydrogen or fluorine.

In certain embodiments, nds of the present invention are represented by formula 12e: R3 R4 RHNMN/WN H H | \/\N R R2 / H wherein, R is hydrogen, -CH3,-CH2CH3, m can be 0 to 7, n can be 0 to 8 and 0 can be 2 to 4, R2 can be independently selected from hydrogen, CH2CH3, and R3 and R4 may be the same or different and are independently selected from hydrogen or fluorine.

Compounds include, but are not d to: H H H NHQ N\/W\ /\/\/\/NH2N H H H2N\/\/N\/\/\NH/mNHZ H H2N\/\/\/N\/\/\N wiped NH2 N W»NodN wwmooooN \ N HZNMNAQVHN\/\/NH2 HZNMN /\©\/N NH N \ \ N I N>H/ HN/\/\N MwowN S “MoodN\/\/\/\ HZNMN NH H H JL NH2 HN2 N/\/\/NH2 Additional disclosure may be found in WOOS/ 105729, the disclosure of which is incorporated by reference as if written herein in its entirety. onal analogs and tives include compounds of the formulas l3a—d: HAHN \/\/ NNNARz R2/\N/\/\/“A“NNAR1 H H R1/\N/\/\/NH\/A.\~‘\”m v N R2 R H H 2\/ WAN/[ll'A/N H NHARl wherein R1 and R2 are independently selected from the group consisting of —C1-C10 alkyl, -C3- C10 cycloalkyl, -C1—C10 alkylene-cycloalkyl, -C6-C10 aryl, and -C1-C10 alkylene-aryl, wherein when both R1 and R2 are alkyl, at least one of R1 and R2 is —C2-C10 alkyl and wherein both R1 and R2 are not tert-butyl; and all salts, hydrates, solvates, and stereoisomers thereof; and all mixtures of stereo isomers thereof, including racemic mixtures. In one embodiment, the substituents on the cyclopropyl ring are trans to each other. In r embodiment, the substituents on the cyclopropyl ring are cis to each other.

In one embodiment, when both R1 and R2 are alkyl, at least one of R1 and R2 is straight-chain alkyl. In another embodiment, when both R1 and R2 are alkyl, both R1 and R2 are straight-chain alkyl. In one embodiment, one of R1 and R2 is -C1-C10 alkyl and the other is -C2-C10 alkyl. In one ment, one of R1 and R2 is -C]-C10, alkyl and the other is —C4-C10 alkyl. In one embodiment, both R1 and R2 are -C4-C10 alkyl. In one embodiment, one of R1 and R2 is -C6-C10 alkyl. In one embodiment, both R1 and R2 are -C6-C10 alkyl. In one embodiment, one of R1 and R2 is -C1-C10 alkyl and the other is ed from the group consisting of -C2—C4 straight-chain alkyl and —C4—C10 alkyl. In another embodiment, R1 and R2 are independently selected from the group consisting of —CH3, -(CH2)3CH3, and - (CH2)sCH3, provided that both R1 and R2 are not —CH3.

In one embodiment, one of R1 and R2 is -C1-C10 alkyl and the other is -C3-C10 cycloalkyl, -C1-C10 alkylene-cycloalkyl, 0 aryl, or -C1-C10 alkylene-aryl. In one embodiment, one of R1 and R2 is —C1-C10 alkyl and the other is - C3—C10 cycloalkyl or -C1-C10 alkylene-cycloalkyl. In one embodiment, one of R1 and R2 is -C1-C10 alkyl and the other is — C3—C10 cycloalkyl. In one embodiment, one of R1 and R2 is —C1-C10 alkyl and the other is -C6— C10 aryl or 0 alkylene-aryl. In one ment, one of R1 and R2 is -C1-C10 alkyl and the other is —C6-C10 aryl. In another embodiment, both R1 and R2 are —C6-C10 aryl. In another embodiment, both R1 and R2 are -C3-C10 cycloalkyl. In one embodiment, the aryl group is benzene. In one embodiment, the cycloalkyl group is adamantyl. In one embodiment, the adamantyl group is l-adamantyl. In r embodiment, the adamantyl group is 2- tyl. In another embodiment, R1 and R2 are independently ed from the group consisting of—CH3, phenyl, and adamantyl, provided that both R1 and R2 are not -CH3.

Compounds include, but are not limited to: W0 2014l110154 >N/\/\/N2ii H30(H20)/\M/V\/NHAHN9 \”5; H3C(HZC)Q/\M/\/\/NH\/A\‘ H H \/ \/\/\ / M NNNA(CHZ)9CH3 Hac(HZC>4/\N/V\/“VA"“\N/\/\/HVH H H \/ N/h N ”MN/\(CHZMCHs [03 90] Additional disclosure may be found in WO2008I112251, the disclosure of which is incorporated by reference as if written herein in its entirety.

Additional s and derivatives include nds of the formula 14a: Rl-X—R2 wherein R1 is H, or is a head group selected from the group consisting of a ht or branched €1.10 aliphatic, alicyclic, single or multi-ring aromatic, single or multiring aryl substituted aliphatic, aliphatic—substituted single or multiring aromatic, a single or multiring cyclic, a single or multiring heterocyclic-substituted aliphatic and an aliphatic-substituted aromatic; R2 is a polyamine; and X is CO, NHCO, NHCS, or $02 In another embodiment of the above ition, R2 has the formula NH(CH2)nNH(CH2)pNH(CH2)qNHR3 wherein n, p and q vary independently and n=p=q=l to 12; and R3 is H; C140 alkyl; €1-10 alkenyl; €1.10 alkynyl; alicyclic; aryl; aryl—substituted alkyl, alkenyl or alkynyl; alkyl-, alkenyl-, or alkynyl-substituted aryl; guanidino; heterocyclic; cyclic-substituted alkyl, alkenyl or alkynyl; and alkyl-, alkenyl-, or l-substituted heterocyclic. [03 93] The above composition may further comprise, linked between X and R2, a linker L and an additional group Y, such that said composition has the formula 14b: Rl-X-L-Y-R2 wherein L is a C140 alkyl; CHO alkenyl; C140 alkynyl, alicyclic, or heterocyclic; X is CO, 802, NHCO or NHCS; and Y is CONH, SO2NH, NHCO, NHCONH, NHCSNH, NHSO2, 802, O, or S.

In the foregoing compositions R1 can have the formula: R,_[:‘ ‘1 wherein R4, R5, R6, R7, and R8 are, independently, H, OH, halogen, N02, (CH)nCH3, N((CH)nCH3)2, CN, (CH)nCH3, O(CH)nCH3, S(CH2)nCH3, NCO(CH2)nCH3, O(CF2)nCF3, or CO-O(CH)nCH3 where n=0 to 10.

Alternatively, R1 has the formula: wherein R4 and R5 are, ndently, H, OH, halogen, NOZ, NH2,NH(CH)nCH3, nCH3)2, CN, (CH)nCH3, O(CH)nCH3, S(CH2)nCH3, NCO(CH2)nCH3, O(CF2)nCF3, or CO-O(CH)nCH3 where n=0 to 10.

In yet another embodiment, R] has the formula: R7'R1}(TA)R4 wherein r and s vary independently and r=s= 0 to 6; R4, R5, R6, R7, R3, and R9 are, independently, H, OH, halogen, NOz, NH2,NH(CH)nCH3, N((CH)nCH3)2, CN, (CH)nCH3, O(CH)nCH3, S(CH2)nCH3, NCO(CH2)nCH3, nCF3, or CO-O(CH)nCH3 where n=0 to 10; Q is CONH, SOZNH, NHCO, , NHCSNH, NHSOZ, 802, O, or S.

Furthermore, R1 may have the formula: RSVR9 {15"R4 wherein r and s vary independently and are 0 to 6; R4, R5, R6, and R7 are, independently, H, OH, N02, NH2,NH(CH)nCH3, N((CH)nCH3)2, CN, (CH)nCH3, O(CH)nCH3, S(CH2)nCH3, NCO(CH2)nCH3, O(CF2)nCF3, or CO-O(CH)nCH3 where n=0 to 10; and Q is CONH, SOZNH, NHCO, NHCONH, NHCSNH, NHSOz, 802, O, or S.

In the foregoing compositions, R1 may be selected from the group ting of naphthalene, phenanthrene, anthracene, pyrene, dibenzofuran, acridine, 2,1,3- benzothiodiazole, quinoline, isoquinoline, benzofuran, indole, carbazole, fluorene, 1,3- benzodiazine, phenazine, phenoxazine, hiazine, adamantane, camphor, piperidine, alkylpiperazine, morpholine, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, thiophene, furan, pyrrole, alkyl—l,2-diazole, alkylimidazole, lH-l ,2,3- triazol, alkyl-lH-l,2,3,4-tetrazole, thiazole, oxazole, thiadiazole, nyl, pyrimidine, 1,2-diazine, l,4-diazine and 1,3,5—triazine, 4-dimethylaminoazobenzene, 3—phenyl methylisooxazole, 3-(2-chlorophenyl)methylisooxazole, 2-(4-chloropheny)methyl chloroquinoline, 6-chloroimidazo[2,l-B]thiazole, (x—methylcinnamic acid, and 2-[l,2-dihydro- 2H— 1 ,4-benzodioxepinyl]thiazole.

R1 may also be a D- or o acid.

Also provided is the above composition where R1 has a formula selected from the group consisting of (A) R12-R13-Y1-R14 (B) R12-Y1-R13-Zi-R14 R6Y1 | \Z’l—R15 (D) /R12 wherein R12 and R13, independently, are H, alene, phenanthrene, anthracene, pyrene, dibenzofuran, acridine, 2,1,3-benzothiodiazole, quinoline, isoquinoline, benzofuran, , carbazole, fluorene, l,3-benzodiazine, phenazine, phenoxazine, phenothiazine, adamantane, camphor, dine, alkylpiperazine, morpholine, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, eptyl, cyclooctyl, thiophene, furan, pyrrole, alkyl-I,2-diazole, midazole, alkyl-lH-1,2,3 -triazol, alkyl-lH-l,2,3,4-tetrazole, thiazole, e, 1,3,4- thiadiazole, pyridinyl, pyrimidine, l,2-diazine, l,4—diazine and 1,3,5-triazine, 4- dimethylaminoazobenzene, 3 -pheny1-5 1isooxazole, 3-(2-chloropheny1)-5 - methylisooxazole, 2-(4-chloropheny)methylchloroquinoline, 6-chloroimidazo[2, l- thiazole, a-methylcinnamic acid, or 2-[1,2—dihydro-2H—l,4-benzodioxepinyl]thiazole; and further, wherein a ring of R12, R13 or both in formulas (A), (B) and (D), is optionally substituted with one or more of OH, halogen, N02, NH2,NH(CH)nCH3, N((CH)nCH3)2, CN, (CH)nCH3, O(CH)nCH3, S(CH2)nCH3, NCO(CH2)nCH3, O(CFz)nCF3, or CO-O(CH)nCH3 where n=0 to 10 R14and R15 and, in formula (C), R13, independently, are (CH2)n, (CH2)nCH=CH, (CH2)n(CH=CH)mCO, or (CH2)nCO where n=0 to 5 and m=1 to 3; Y1, and 21, independently, are CONH, SOZNH, NHCO, NHCONH, NHCSNH, NHSOz, SOZ, 802, O, S, or COO; when R1 is of formula (A) or (B), Y1 represents a bond between a C or N atom of R12, and a C or N atom of R13, and 21 represents a bond between a C or N atom of R13, and a C or N atom of R14; or when R1 is of formula (C) or Y1 represents a bond between the C and a C or N atom of R13 and 21 represents a bond between the C and a C or N atom of R14; or when R1 is of formula (D) Y1 represents a bond between a C or N atom of R12 and a C or N atom of R14 and 21 ents a bond between a C or N atom of R13 and a C or N atom of R15.

In the above compositions, R2 preferably has the formula 1)(CH2)nNH(CH2)pNH(CH2)qCH(Z1)NHR3 n, p and q vary independently and 1 to 12; and R3 is H; C140 alkyl; C1_10 alkenyl; CHO alkynyl; alicyclic; aryl; aryl-substituted alkyl, alkenyl or alkynyl; alkyl-, alkenyl—, or alkynyl-substituted aryl; guanidine or heterocyclic; Z, is CH3, CHZCH3or cyclopropyl.

In another embodiment, R2 has the formula: N N R10/ ‘6‘); \R1 1 wherein x=l to 4; y=l to 3, R10 and R11 are, independently, H, (CH2)nNHR12 or (CH2)kNH(CH2)1 NHR12, where n=k=l=1 to 10, and R12 is H or C(N=H)NH2.

In the above compositions, R2 is preferably selected from the group consisting of N1-acety1spcrmine, N1 -acetylspermidine, N8-acety1spermidine, N'-guanidinospermine, cadaverine, aminopropylcadaverine, homo dine, caldine (horspermidine), 7- hydroxyspermidine, thermine (norspermine), thermospermine, canavalmine, aminopropylhomospermidine, N,N‘—bis(3-amin0pr0pyl)cadaverine, aminopentylnorspermidine, N4-aminopropylnorspermidine, N4-amin0pr0pylspermidine, entamine, homocaldopentamine, N4—bis(amin0pr0py1)norspermidine, thermopentamine, N4-bis(aminopropy1)spermidine, caldohexamine, homothermohexamine, ldohexamine, N-(3—amin0pr0py1)-1,3-pr0panediamine, N,N‘—bis(3- aminopropyl)ethylendiamine, N,N'-bis(3-amin0pr0py1)-1,4-piperazine, is(3- aminopropy1)-1 ,3-piperazine, N,N‘-bis(3-amin0pr0py1)-1,3-pr0panediamine, N,N’-bis(2- aminoethyl)-1,3-pr0panediamine, tris(3 -amin0pr0py1)amine, and tris(aminoethyl)amine.

Compounds include, but are not limited to: W0 10154 WO 10154 W0 10154 W0 2014l110154 S\\O O\\ HN NI OI/S\NH HN NH NH HN M NH NH2 HN HN O / M NH NH2 W0 2014l110154 HN HN NH H2N NH \S/IO\\O S OvS HN HN \ .l \NH NH NH HN HN M NH w2 09Oflm HN i NH NH NH HN HN NH M NH N um.

/ S / HN, HN SMO NH NH2 / \| HN HN O NH NH2 W0 10154 H H \ W\/\/N\/\/\NH2N N KANO H H H/\n/N\/\/N\/\/\N/\/\NH2H WO 10154 W0 10154 NH H2 WO 10154 A? H H H H H H H R H N H2N/\/ \/\n/ W /\/\NH2 E H H HZNWNWNNNMNHZ /\ O H H s ”MNWNNNMNHZH W0 10154 H2N HN NH W2 H2NWHN M NH NH2 H2N HN WA NH NH2 H2NVNA 30O\\ \ 01/qu O N| NH Naw.

O// \fiO 098 HN HN m \NH I B N| NH O 0 0/ \ WS HN HN N O \NH NH2 0 0980/! \ N| O \ HN HN NH NH N H2N HN HN I, NH NH O H HN HN S HN2 NH ,,. o H H (1:1)“ GEE) C331? N— N— N— / / / (130% W, ? WowO Additional disclosure may be found in WO99/03823, the sure of which is incorporated by reference as if written herein in its entirety.

Yet further compounds include, but are not limited to: NH / NH I H NH N \ \ NH «N NHz 2 I H2N \ N H2N N \n/ | HZNJLN NH H / NH <filN O N H2N/E/\Ncm N HZN/VI’ICHFZNHz, HzN/T\O,NH2 HO OH HO 0 onal disclosure may be found in: Ackermann, JM; Pegg, AE; McCloskey, DE; ss in Cell Cycle Research, 2003, Vol. 5, 461-468; Ekelund, S; , P; Larsson, R; Biochemical Pharmacology, 2001, 61, 1183—1193; Huang, Y; Pledgie, A; Casero Jr, RA; Davidson, NE; Anti-Cancer Drugs, 2005, 16, 229—241; and Marton, JL; Annu. Rev.

Pharmacol Toxicol. 1995, 35, 55-91; the disclosure of which is incorporated by reference as if written herein in their entireties.

Polyamine analogs depicted above may be prepared both as salts and as free bases. In n embodiments, the salt is the hloride salt. In certain embodiments, the number of coordinated ion pairs (for example H+Cl‘) will be proportional to the number of amino groups in the polyamine. Such coordination typically occurs at said amino groups, forming, for example, NH3+C1' groups. However, not every amino group may be coordinated. For example, if the amino group is adjacent to an electron-withdrawing group such as carbonyl or sulfonyl, it may not retain sufficient electron density to coordinate an ion.

In further embodiments, the number of coordinated ions will be proportional to the number of primary and/or ary amino groups in the polyamine.

Additional compounds which may be used in the methods and itions described herein include: naturally occurring polyamines found in prokaryotes and eukaryotic cells, polyamine analogs, polyamine biosynthesis inhibitors, and polyamine transport inhibitors.

Naturally occurring polyamines found in yotes and eukaryotic cells include, but are not limited to: putrescine, dine, spermine, diaminopropane, cadaverine, norspermidine, aminopropylcadaverine, homospermine, norspermine, thermospermine, entylnorspermidine, bis(aminopropyl)cadaverine, aminopropylhomospermine, 30 canavalmine, homospermine, caldopentamine, aminopropylcanavaline, inopropyl)homospermidine, inobutyl)norspermidine, aminobutylcanavalmine, aminopropylhomospermine, homopentamine, N5-aminobutylhomospermine, caldohexamine, hexamine, homothermohexamine, agmatine and N6—methylagmatine. See, e. g., Morgan D. M. L., 1999, Molecular Biotechnology 11: 229.

Polyamine s include, but are not limited to, BE-4444 [1,19-bis (ethylamino)-5,10,15-triazanonadecane]; BE3-3 [N1,N1l-diethylnorspermine; DENSPM; 1,11-bis (ethylamino)-4,8-diazaundecane; thermine; Warner-Parke-Davis]; BE3[N1,N7- bis(ethyl) norspermidine]; [N1,N8-bis(ethyl) spermidine]; BE44 [N1,N9-bis(ethyl) homospermidine]; BE-343 [N1,N12-bis(ethyl) spermine; diethylspermine—Nl-N12; DESPM]; BE-373 [N,N'—bis (3-ethylamino) propyl)-1,7-heptane diamine, Merrell-Dow]; BE-4—4-4 [N1,N14—bis(ethyl) homospermine; diethylhomospermine—Nl-Nl-l]; BE4—4-3 [1,17- bis(ethylamino)-4,9,14triazaheptadecane]; BE34 [l,17—bis(ethylamino)-5,9, 13— triazaheptadecane]; and 1,12-Mez—SPM [1,12-dimethylspermine]. (WOO2007/040535). ine synthesis inhibitors include but are not d to: inhibitors of ornithine decarboxylase such as DFMO, aceylenic putrescine, 1-aminooxy—3-aminopropane, antizyme, 2-butylputrescine, cadaverine, L—canaline, 5'—deoxy-5‘-[N—methyl—N- [3(aminooxy)ethyl]amino]adenosine, 5'—deoxy-5'—[N-methyl-N-[3- (hydrazinopropyl)amino]adenosine, opropane, 1,3-diamino-2—propanol, 2- difluoromethyl putrescine, difluorophenylethy1(4-aminopropylamidinohydrazone), 2,3- dimethylputrescine, N-dimethylputrescine, 2-ethy1putrescine, (+ or —)- alphafluoromethylornithine, 2-fluoro methylputrescine, 2-hexy1putrescine, 2- hydrazinoornithine, ibuprofen, yl acetylenic putrescine, methylglyoxal bis(3aminopropylamininohydrazone), 2-methylornithine, ylputrescine, 2- monofluoromethyl-trans-dehydorornithine, 2-monofluoromethy1 dehydroputrescine, monofluoromethylomithine, 2-monofluoron1ethy1putrescine, neomycin, D-omithine, 2- pentylputrescine, p-phenylenediamine, phosphopeptide MG 25000, phosphothreonine, phosphotyrosine, 2-propy1putrescine, putrescine, allo-S-adenosyl-L—methionine, S- hioadenosine, thioadenosine, and 5' —methy1—thioadenosine as discussed in Zollner H. (1993) Handbook of Enzyme Inhibitors, 2nd Ed. Weinheim:Base1(Switzer1and); inhibitors of S-adenosylmethionine decarboxylase, such as SAM486A (4-aminoindanon-1(2‘ amidino)hydrazone dihydrochloride monohydrate), S-adenosyl-1,8—diamino—3thiooctane, S- enosyl)methylthioaminooxyethan, S-adenosy1methylthio-i-propylamine, 5‘-{ [(2)- 4—amino-2—butenyl]methylamino}—5'—deoxyadenosine, 5'—amino-5'deoxyadenosine, 5‘- [(aminoiminomethy1)amino]-5']deoxyadenosine dihydrogensulphate, 1-aminooxy aminopropane, [2—(aminooxy)ethyl](5'—deoxyadenosine-5'y1)(methy1)su1phonium, 5'—[(3- aminopropyl]-amino)-5'—deoxyadenosine, 5‘-[(3aminopropy1]-n1ethy1amino)-5'— denosine, 9—[6(RS)—an1ino-5,6,7-trideoxy-beta-D-ribo—octofuranosyl]—9H—purin amine, borohydride, n-butylglyoxal bis(guany1hydrazone), 9-[6(RS)—c-carboxan1ido-5,6,7- trideoxy-beta-D-ribo-octofuranosy1]-9H-purinamine, cyanide, cyanoborohydride, S-(5' deoxy-5'adenosy1)methionylethylhydroxylamine, S-(5' deoxy- 'adenosyl)methionylthiohydroxylamine, 5‘ -deoxy-5‘-[N-methyl-N- [2(an1inooxy)ethyl]amino]adenosine, 9-[6(S)-diamino-5,6,7,8,9-pentadeoxy-beta-D—ribo- nanofuranosyl]—9H-purin—6-amine, diethylglyoxal bis(guany1hydrazone), ophynylethyl (4-aminopropylamidinohydrazone), dimethy1(5' -adenosy1jsu1fonium, dimethylglyoxal bis(guany1hydrazone), ethylglyoxal bis(guany1hydrazone), hydroxylamine, 4- ypenenal, MDL 73811, 5'[[3-methy1an1ino)propy1]amino]-5'—deoxyadenosine(1,1'- (methylethanediylidine)dinitro)bis(3-aminoguanididne), methylglyoxal bis(3- aminopropylamidinohydrazone), glyoxal bis(cyclohexylamidinohydrazone), pentanedialdehyde bis(guany1hydrazone), phenylhydrazine, propanedialdehyde bis(guany1hydrazone), semicarbazide, sodium borohydride, sodium cyanoborohydride, and spermine as discussed in Zollner H. (1993) Handbook of Enzyme Inhibitors, 2nd Ed.

Additional disclosure may be found in W02002/053519, the disclosure of which is incorporated by nce as if written herein in its entirety.

Additional spermine analogs include N—(2—mercaptoethyl)spermine carboxamide (MESC), the disulfide from thereof, namely 2,2 1-dithiobis(N-ethyl-spermine- —carboxamide) (DESC), and N-[2,2,1-dithio(ethyl l,1-aminoethyl)]spermine—S-carboxamide (DEASC). (WO98/17623) Polyamine effectors that are small molecule inhibitors or modulators of key enzymes in the polyamine biosynthetic pathway e, but are not limited to: ODC inhibitors such as difluoromethylomithine (DFMO), alpha-monofluoromethylomithine (MFMO), and methyl acetylenicputrescine (MAP); AdometDC inhibitors such as S-(5- deoxy-5adenoxyl)methyIthioethylhydroxylamine (AMA), y [(2aminooxyethyl)methyllamino]adenosine (MAOEA), and methylglyoxal bis(guanylhydrazone) (MGBG); spermidine se tors such as S-adenosyll,8- diamino-3—thiooctane (AdoDATO), cyclohexylamine, and butylamine; spermine synthase inhibitors such as osyl-l,12—diaminothio—9-azadodecane (AdoDATAD) and N-(n- butyl)-1 ,3—diaminopropane (BDAP).

In certain embodiments, the polyamine effector is a ine or arginine analog that s a functional group that confers a cellular or DNA protective effect to the le, or that modulates the polyamine biosynthetic or catabolic y. nds of this nature include, but are not limited to amifostine, NG-hydroxy-arginine (NORA), N1, N11-bis(ethyl) norspermine (BE3-3), N12-bis(ethyl)spermine (BE4-3), N,N-bis[3- (ethylamino)-propyl]-1 ,7heptanediamine (BE7—3), BE-3—3—3, BE—33, BE7—3,N1- ethyl-N1 l-propargyl 4,8-diazaundecane, and the analogs SL-11141 and SL-11050 (structures set forth in one or more of US. Patent 061, nas et aI., 2001,supra, WO 00/66587 and WO 02/38105). Additional disclosure may be found in W003/013245, the disclosure of which is orated by reference as if written herein in its entirety.

As used herein, the terms below have the meanings indicated.

The term “cytokine,” as used herein, alone or in combination, means signaling molecules secreted by cells of the immune system which have a local immunoregulatory effect. Cytokines may include, without limitation, IL-l, ILl-Ra, IL—2, IL-6, 1L8, IFNy, IP- , IL-17, MCP-l, MMP-9, MIP-lB, TNF-(x, TGFB, CRP, OPN, and RANTES.

When ranges of values are disclosed, and the notation “from n1 to n2” or “between n1 and n2” is used, where n1 and n2 are the numbers, then unless otherwise 2014/010714 specified, this notation is intended to include the numbers themselves and the range between them. This range may be integral or continuous between and including the end . By way of example, the range “from 2 to 6 carbons” is intended to include two, three, four, five, and six carbons, since carbons come in r units. Compare, by way of example, the range “from 1 to 3 uM (micromolar),” which is intended to e 1 uM, 3 uM, and everything in between to any number of significant figures (e. g., 1.255 trM, 2.1 pM, 2.9999 uM, etc.).

The term “about,” as used herein, is intended to qualify the cal values which it modifies, denoting such a value as variable within a margin of error. When no particular margin of error, such as a standard deviation to a mean value given in a chart or table of data, is recited, the term ” should be understood to mean that range which would encompass the recited value and the range which would be included by rounding up or down to that figure as well, taking into account significant figures.

The term “substantially” as used herein is intended to mean predominantly or having the overriding characteristic of, such that any opposing or detracting characteristics reach a level of insignificance. By way of example, a composition antially” free of water might not be absolutely free of all traces of water, but would be sufficiently anhydrous that any ing water would not influence the composition in any significant way. By way of further e, “substantially dose-limiting side effects” might be side effects which limited a dose to a level which was below that ed for therapeutic efficacy.

The term “disease” as used herein is intended to be generally synonymous, and is used interchangeably with, the terms “disorder,77 LCsyndrome,” and “condition” (as in l condition), in that all reflect an abnormal condition of the human or animal body or of one of its parts that impairs normal functioning, is typically manifested by distinguishing signs and symptoms, and causes the human or animal to have a reduced duration or quality of life.

A “proliferative disorder” may be any disorder characterized by dysregulated cellular eration. Examples include cancers, psoriasis, and atopic dermatitis.

As used herein, “hyperalgesia” means a heightened sensitivity to pain, and can be considered a type of pain or a measure of pain-related behavior.

As used herein, “progressive” multiple sclerosis refers to forms of the e which progress towards an ever-worsening disease state over a period of time. Progressive MS includes, for example, primary progressive MS, secondary progressive MS, and progressive relapsing MS. These subtypes may or may not feature episodic flare-ups of the e, but are each associated with increased symptoms, such as increased demyelination or pain and reduced capacity for movement, over time.

As used herein, reference to "treatment" of a patient is intended to include prophylaxis. Treatment may also be preemptive in nature, i.e., it may include prevention of disease. Prevention of a disease may involve complete protection from disease, for example as in the case of tion of infection with a pathogen, or may involve prevention of disease progression. For example, prevention of a disease may not mean te foreclosure of any effect related to the es at any level, but instead may mean prevention of the symptoms of a disease to a clinically significant or detectable level. Prevention of diseases may also mean prevention of progression of a disease to a later stage of the disease.

The term "combination therapy" means the administration of two or more therapeutic agents to treat a therapeutic condition or disorder described in the present disclosure. Such administration encompasses inistration of these therapeutic agents in a substantially simultaneous manner, such as in a single capsule having a fixed ratio of active ingredients or in multiple, separate capsules for each active ient. In on, such administration also encompasses use of each type of therapeutic agent in a sequential manner.

In either case, the treatment regimen will provide beneficial effects of the drug combination in treating the conditions or disorders described herein.

The term “patient” is generally synonymous with the term “subject” and means an animal differing from a disease, disorder, or condition ble in accordance with the methods sed herein, including all mammals and humans. Examples of patients include humans, livestock such as cows, goats, sheep, pigs, and rabbits, and companion animals such as dogs, cats, rabbits, and horses. Preferably, the patient is a human.

An “effective amount” or a peutically effective amount” is a quantity of a compound (e.g., MGBG, a ine analog, a polyamine thesis inhibitor or any agent) that is sufficient to e a desired effect in a subject being treated. For instance, this can be the amount necessary to treat a disease, disorder, condition, or adverse state (such as pain or inflammation) or to otherwise measurably alter or alleviate the ms, markers, or mechanisms of the e, disorder, condition, or adverse state. As just one example, an effective amount for the treatment of pain is an amount ient to t, delay the onset of, or reduce pain or one or more pain-related symptoms in a subject, as measured by methods known in the art. Similar methods of assessing se to treatment of a number of diseases are well—know in the art. The effective amount of a compound of the present invention may vary depending upon the route of administration and dosage form. In addition, specific dosages may be adjusted depending on conditions of disease, the age, body weight, general health ions, sex, and diet of the subject, dose intervals, administration routes, excretion rate, and combinations of agents.

The term “low dose,” in reference to a low dose formulation of a drug or a method of ent specifically employing a “low dose” of a drug, means a dose which for at least one indication is subtherapeutic, or is a fraction of the dose typically given for at least one indication. Take for example the case of a drug for the ent of proliferative disorders — a low dose formulation for the treatment of, say, multiple sclerosis, might be a fraction of the dose for the treatment of an aggressive cancer. In this way, the dose for one disease might be an amount which would be subtherapeutic for another disease. Alternatively, for a drug which is eutic in different individuals or populations at different doses, and is available in a range of doses, a low dose may be simply a dose toward the low end of recognized therapeutic efficacy. Chronic diseases represent an embodiment treatable by low dose formulations and methods. Additionally, a subtherapeutic amount of a drug might be used in combination with one or more other drugs (themselves in either therapeutic or subtherapeutic amounts) to yield a combination formulation or treatment which is potentiated, that is, more efficacious than the expected effects of the sum of the drugs given alone. A low dose for the treatment of one indication may be two-fold, fold, four-fold, old, six-fold, seven- fold, eight—fold, nine-fold, ten-fold, n-fold, twenty-fold, thirty-fold, forty-fold, fifty-fold, may be one hundred-fold less than the therapeutic dose for a different indication.

The phrase "therapeutically effective" is intended to qualify the amount of active ingredients used in the treatment of a disease or er or on the effecting of a clinical endpoint.

The term “therapeutically acceptable” refers to those compounds (or salts, prodrugs, tautomers, rionic forms, etc.) which are le for use in contact with the tissues of subjects without undue toxicity, irritation, and allergic response, are commensurate with a reasonable benefit/risk ratio, and are ive for their ed use.

The term “drug” is used herein interchangeably with “compound” and “agent.” When a compound is ed to herein as “not a T-cell regulator,” what is meant is that any direct activity against T—cells is negligible and/or secondary to activity attributable to another leukocyte subtype. In certain embodiments, a cell which is “not a T-cell regulator” will be a cell of myeloid lineage. In certain ments, such a cell will be a dendritic cell, a monocyte, or a macrophage.

The phrase “reduced nce of at least one side effect” as used herein means reduced to a degree that is significant. Significance can be demonstrated by statistical methods (i.e., by non-overlapping standard deviations or appropriate confidence intervals).

Significance of reduced nce of side s may also be demonstrated by nce to ative es such as, for example, the y to achieve or in a therapeutic dose without dose—limiting toxicity (in all ts or in a patient subpopulation), the ability to prevent or delay disease relapse or progression, or patient preference.

The phrase “approved for the treatment of a demyelinating disease,” as used herein, means ed by a drug regulatory agency (in the United States, Europe or any EPO country, Japan, Canada, or Australia) for the treatment of a demyelinating disease. In any embodiment disclosed herein, the demyelinating disease may be, specifically, le sclerosis.

The term “SAMDC inhibitor” means an inhibitor of the enzyme osyl methionine decarboxylase. MGBG is believed to be one such SAMDC inhibitor, and other polyamines, polyamine analogs, and polyamine biosynthesis inhibitors may also be SAMDC inhibitors.

As used herein, a “polyamine” is any of a group of aliphatic, straight-chain amines derived biosynthetically from amino acids; ines are reviewed in Marton et al. (1995) Ann. Rev. Pharm. Toxicol. 35:55-91. By “polyamine” is generally meant a naturally— occurring polyamine or a polyamine which is naturally produced in eukaryotic cells.

Examples of polyamines include putrescine, spermidine, spermine and cadaverine.

As used herein, a “polyamine analog” is an organic cation structurally similar but non-identical to naturally-occurring polyamines such as spermine and/or spermidine and their precursor, diamine putrescine. Polyamine s can be ed or un-branched, or incorporate cyclic moieties. Polyamines may comprise primary, secondary, tertiary, or quaternary amino groups. In one embodiment, all the nitrogen atoms of the polyamine analogs are independently secondary, tertiary, or quaternary amino , but are not so limited. Polyamine analogs may include imine, amidine and guanidine groups in place of amine groups. The term “polyamine analog” includes stereoisomers, salts and protected derivatives of polyamine analogs.

A “stereoisomer” is any optical isomer of a compound, including enantiomers and diastereomers. Unless otherwise indicated, structural formulae of compounds are intended to embrace all possible stereoisomers.

A “salt” or “pharmaceutically acceptable salt” is a compound formed by the replacement of one or more hydrogen atoms with ts or groups, which is composed of anions and s, which usually ionizes in water; a salt is formed, for instance, by neutralization of an acid by a base. es of salts include, but are not limited to, halide, for example, chloride, bromide, or , nitrate, sulfate, bisulfate, phosphate, acid phosphate, isonicotinate, acetate, lactate, salicylate, citrate, tartrate, pantothenate, bitartrate, ascorbate, succinate, maleate, gentisinate, fumarate, gluconate, glucaronate, saccharate, formate, benzoate, glutamate, methanesulfonate, ethanesulfonate, benzensulfonate, p- toluenesulfonate and pamoate (i.e., 1,1'—methylene—bis-(2-hydroxy- 3—naphthoate)) salts.

“Protected derivative” is used to refer to a compound protected with a protecting group. cting group” refers to a chemical group that exhibits the following characteristics: 1) reacts selectively with the desired functionality in good yield (preferably at least 80%, more preferably at least 90%, more preferably at least 95%, still more preferably at least 99%) to give a protected substrate that is stable to the projected reactions for which protection is desired; 2) is selectively removable from the protected substrate to yield the desired onality; and 3) is removable in good yield rably at least 80%, more preferably at least 90%, more preferably at least 95%, still more preferably at least 99%) by reagents compatible with the other functional group(s) present or generated in such projected ons. Examples of suitable protecting groups can be found in Greene et a1. (1991) Protective Groups in c Synthesis, 2nd Ed. (John Wiley & Sons, Inc., New York).

Exemplary protecting groups for the amino functionality include, but are not limited to, lenesulfonyl (MesSOz ) t- , benzyloxycarbonyl (CBz), loxycarbonyl (Boc), butyldimethylsilyl (TBDIMS), enylmethyloxycarbonyl (Fmoc), or suitable photolabile protecting groups such as 6—nitroveratryloxy carbonyl (Nvoc).

The term “acyl,” as used , alone or in combination, refers to a carbonyl attached to an alkenyl, alkyl, aryl, cycloalkyl, heteroaryl, heterocycle, or any other moiety were the atom attached to the carbonyl is carbon. An l” group refers to a —C(O)CH3 group. An “alkylcarbonyl” or “alkanoyl” group refers to an alkyl group attached to the parent molecular moiety through a carbonyl group. Examples of such groups include methylcarbonyl and ethylcarbonyl. Examples of acyl groups e formyl, alkanoyl and aroyl.

The term yl,” as used herein, alone or in combination, refers to a straight— chain or branched—chain hydrocarbon radical having one or more double bonds and containing from 2 to 20 carbon atoms. In certain embodiments, said alkenyl will comprise from 2 to 6 carbon atoms. The term ylene” refers to a carbon—carbon double bond system attached at two or more positions such as ethenylene [(—CH=CH—),(—C::C—)].

Examples of suitable alkenyl radicals include ethenyl, propenyl, 2-methylpropenyl, 1,4- butadienyl and the like. Unless otherwise specified, the term “alkenyl” may e “alkenylene” .

The term “alkoxy,” as used herein, alone or in combination, refers to an alkyl ether l, wherein the term alkyl is as defined below. Examples of suitable alkyl ether radicals include y, ethoxy, n-propoxy, isopropoxy, n-butoxy, iso-butoxy, sec-butoxy, tert—butoxy, and the like.

The term “alkyl,” as used herein, alone or in combination, refers to a straightchain or branched-chain alkyl radical ning from 1 to 20 carbon atoms. In certain embodiments, said alkyl will comprise from 1 to 10 carbon atoms. In further embodiments, said alkyl will comprise from 1 to 6 carbon atoms. Alkyl groups may be ally tuted as defined herein. es of alkyl radicals include methyl, ethyl, n-propyl, isopropyl, l, isobutyl, sec—butyl, tert—butyl, pentyl, yl, hexyl, octyl, nonyl and the like. The term “alkylene,” as used herein, alone or in combination, refers to a saturated aliphatic group derived from a straight or branched chain saturated hydrocarbon attached at two or more positions, such as methylene (—CH2—). Unless otherwise specified, the term “alkyl” may include “alkylene” groups.

The term “alkylamino,” as used herein, alone or in ation, refers to an alkyl group attached to the parent molecular moiety through an amino group. Suitable alkylamino groups may be mono- or dialkylated, forming groups such as, for e, N-methylamino, N-ethylamino, N,N-dimethylamino, N,N-ethylmethylamino and the like.

The term “alkynyl,” as used herein, alone or in combination, refers to a straight- chain or branched chain hydrocarbon radical having one or more triple bonds and ning from 2 to 20 carbon atoms. In certain embodiments, said alkynyl comprises from 2 to 6 carbon atoms. In further embodiments, said alkynyl comprises from 2 to 4 carbon atoms.

The term “alkynylene” refers to a carbon-carbon triple bond attached at two positions such as ethynylene (—C:::C—, —CEC—). Examples of alkynyl radicals include ethynyl, propynyl, hydroxypropynyl, butyn—l-yl, butynyl, pentyn—l-yl, 3—methylbutyn-l-yl, hexyn—2-yl, and the like. Unless otherwise specified, the term “alkynyl” may include “alkynylene” groups.

The terms “amido” and “carbamoyl,”as used herein, alone or in combination, refer to an amino group as described below attached to the parent molecular moiety through a carbonyl group, or Vice versa. The term “C-amido” as used herein, alone or in combination, refers to a —C(O)N(RR’) group with R and R’ as defined herein or as d by the specifically enumerated “R” groups designated. The term “N-amido” as used herein, alone or in combination, refers to a RC(O)N(R’)- group, with R and R’ as defined herein or as defined by the specifically enumerated “R” groups designated. The term "acylamino" as used herein, alone or in combination, embraces an acyl group attached to the parent moiety through an amino group. An example of an "acylamino" group is acetylamino (CH3C(O)NH—).

The term “amino,” as used herein, alone or in combination, refers to —NRR7, wherein R and R’ are independently ed from the group consisting of hydrogen, alkyl, acyl, heteroalkyl, aryl, cycloalkyl, heteroaryl, and cycloalkyl, any of which may themselves be optionally substituted. Additionally, R and R’ may combine to form heterocycloalkyl, either of which may be optionally substituted.

The term "aryl," as used herein, alone or in combination, means a carbocyclic aromatic system containing one, two or three rings wherein such polycyclic ring systems are fused together. The term "aryl" embraces aromatic groups such as phenyl, naphthyl, anthracenyl, and phenanthryl.

The term “arylalkyl” or “aralkyl,” as used herein, alone or in combination, refers to an aryl group attached to the parent molecular moiety through an alkyl group. The term “carboxyl” or “carboxy,” as used herein, refers to —C(O)OH or the corresponding “carboxylate” anion, such as is in a carboxylic acid salt. An “O-carboxy” group refers to a — group, where R is as defined herein. A “C-carboxy” group refers to a R groups where R is as defined herein.

The term “cyano,” as used herein, alone or in combination, refers to —CN.

The term “cycloalkyl,” or, alternatively, cycle” or “alicyclic,” as used herein, alone or in combination, refers to a saturated or partially saturated monocyclic, bicyclic or tricyclic alkyl group wherein each cyclic moiety contains from 3 to 12 carbon atom ring members and which may optionally be a benzo fused ring system which is optionally substituted as defined herein. In certain embodiments, said lkyl will comprise from 5 to 7 carbon atoms. Examples of such cycloalkyl groups include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, ydronapthyl, indanyl, octahydronaphthyl, 2,3-dihydro—lH-indenyl, adamantyl and the like. lic” and “tricyclic” as used herein are intended to include both fused ring systems, such as dronaphthalene, dronaphthalene as well as the multicyclic (multicentered) saturated or partially unsaturated type. The latter type of isomer is exemplified in general by, bicyclo[l,l,l]pentane, camphor, adamantane, and bicyclo[3,2,l]octane.

The term “halo,” or “halogen,” as used , alone or in ation, refers to fluorine, chlorine, e, or iodine.

The term "heteroalkyl," as used herein, alone or in combination, refers to a stable straight or branched chain, or cyclic hydrocarbon radical, or combinations thereof, fully ted or containing from 1 to 3 degrees of unsaturation, consisting of the stated number of carbon atoms and from one to three atoms selected from the group consisting of O, N, and S, and wherein the nitrogen and sulfur atoms may optionally be oxidized and the nitrogen heteroatom may optionally be quaternized. The heteroatom(s) O, N and S may be placed at any interior position of the heteroalkyl group. Up to two heteroatoms may be consecutive, such as, for example, -CH2-NH-OCH3.

The term "heteroaryl," as used herein, alone or in combination, refers to a 3- to -membered unsaturated heteromonocyclic ring, or a fused clic, bicyclic, or tricyclic ring system in which at least one of the fused rings is aromatic, which contains at least one atom selected from the group consisting of O, S, and N. In certain embodiments, said aryl will comprise from 5 to 7 carbon atoms. The term also embraces fused polycyclic groups wherein heterocyclic rings are fused with aryl rings, wherein heteroaryl rings are fused with other heteroaryl rings, wherein heteroaryl rings are fused with heterocycloalkyl rings, or wherein heteroaryl rings are fused with lkyl rings. Examples of heteroaryl groups include pyrrolyl, pyrrolinyl, imidazolyl, pyrazolyl, pyridyl, pyrimidinyl, pyrazinyl, pyridazinyl, triazolyl, pyranyl, furyl, thienyl, oxazolyl, isoxazolyl, oxadiazolyl, thiazolyl, thiadiazolyl, azolyl, indolyl, olyl, zinyl, benzimidazolyl, quinolyl, isoquinolyl, quinoxalinyl, quinazolinyl, lyl, benzotriazolyl, benzodioxolyl, benzopyranyl, benzoxazolyl, benzoxadiazolyl, hiazolyl, benzothiadiazolyl, benzofuryl, benzothienyl, chromonyl, coumarinyl, benzopyranyl, tetrahydroquinolinyl, tetrazolopyridazinyl, tetrahydroisoquinolinyl, thienopyn'dinyl, furopyridinyl, pyrrolopyridinyl and the like. Exemplary lic heterocyclic groups include olyl, benzidolyl, phenanthrolinyl, dibenzofuranyl, nyl, phenanthn'dinyl, xanthenyl and the like.

The terms “heterocycloalkyl” and, interchangeably, “heterocycle,” as used herein, alone or in combination, each refer to a saturated, lly unsaturated, or fully unsaturated monocyclic, bicyclic, or tricyclic heterocyclic group containing at least one heteroatom as a ring member, wherein each said heteroatom may be independently selected from the group consisting of nitrogen, oxygen, and sulfur In n embodiments, said hetercycloalkyl will comprise from 1 to 4 heteroatoms as ring members. In r embodiments, said hetercycloalkyl will comprise from 1 to 2 heteroatoms as ring members. In certain embodiments, said hetercycloalkyl will comprise from 3 to 8 ring members in each ring. In further embodiments, said hetercycloalkyl will comprise from 3 to 7 ring members in each ring. In yet further embodiments, said hetercycloalkyl will se from 5 to 6 ring members in each ring. “Heterocycloalkyl” and “heterocycle” are ed to include sulfones, sulfoxides, N-oxides of tertiary nitrogen ring members, and carbocyclic fused and benzo fused ring systems; additionally, both terms also include systems where a cycle ring is fused to an aryl group, as defined , or an onal heterocycle group.

Examples of heterocycle groups include aziridinyl, azetidinyl, nzodioxolyl, dihydroisoindolyl, dihydroisoquinolinyl, dihydrocinnolinyl, dihydrobenzodioxinyl, dihydro[l,3]oxazolo[4,5-b]pyridinyl, benzothiazolyl, dihydroindolyl, dihy-dropyridinyl, 1,3- dioxanyl, l,4-dioxanyl, 1,3-dioxolanyl, isoindolinyl, morpholinyl, zinyl, pyrrolidinyl, tetrahydropyridinyl, piperidinyl, thiomorpholinyl, and the like. The heterocycle groups may be optionally substituted unless specifically ited.

The term “lower,” as used herein, alone or in a combination, where not ise specifically defined, means containing from 1 to and including 6 carbon atoms.

The term “sulfonyl,” as used herein, alone or in combination, refers to —.

Any definition herein may be used in combination with any other definition to describe a composite structural group. By convention, the trailing element of any such definition is that which attaches to the parent moiety. For e, the composite group alkylamido would represent an alkyl group attached to the parent molecule through an amido group, and the term alkoxyalkyl would represent an alkoxy group attached to the parent molecule through an alkyl group.

When a group is defined to be “null,” what is meant is that said group is absent.

The term “optionally substituted” means the anteceding group may be substituted or unsubstituted. When substituted, the substituents of an “optionally substituted” group may include, t limitation, one or more substituents independently selected from the following groups or a particular designated set of groups, alone or in combination: lower alkyl, lower alkenyl, lower alkynyl, lower yl, lower heteroalkyl, lower heterocycloalkyl, lower haloalkyl, lower haloalkenyl, lower kynyl, lower perhaloalkyl, lower perhaloalkoxy, lower cycloalkyl, phenyl, aryl, aryloxy, lower alkoxy, lower haloalkoxy, oxo, lower acyloxy, carbonyl, yl, lower alkylcarbonyl, lower carboxyester, lower carboxamido, cyano, hydrogen, halogen, hydroxy, amino, lower alkylamino, arylamino, amido, nitro, thiol, lower alkylthio, lower haloalkylthio, lower perhaloalkylthio, arylthio, sulfonate, sulfonic acid, trisubstituted silyl, N3, SH, SCH3, C(O)CH3, C02CH3, COZH, pyridinyl, thiophene, furanyl, lower carbamate, and lower urea. Two substituents may be joined together to form a fused five-, six—, or seven-membered carbocyclic or heterocyclic ring consisting of zero to three heteroatoms, for example forming methylenedioxy or ethylenedioxy. An optionally substituted group may be unsubstituted (e. g., —CH2CH3), fully substituted (e. g., —CF2CF3), monosubstituted (e. g., 2F) or substituted at a level anywhere in-between fully substituted and monosubstituted (e. g., -CH2CF3). Where substituents are recited without qualification as to substitution, both substituted and unsubstituted forms are encompassed. Where a substituent is qualified as “substituted,” the substituted form is specifically intended. Additionally, different sets of optional tuents to a particular moiety may be d as needed; in these cases, the optional tution will be as defined, often immediately following the phrase, “optionally substituted wit .” The term R or the term R’, appearing by itself and without a number designation, unless otherwise defined, refers to a moiety selected from the group consisting of hydrogen, alkyl, cycloalkyl, heteroalkyl, aryl, heteroaryl and heterocycloalkyl, any of which may be optionally substituted. Such R and R’ groups should be understood to be optionally substituted as defined herein. Whether an R group has a number designation or not, every R group, including R, R’ and RH where n=(l, 2, 3, ...n), every substituent, and every term should be understood to be independent of every other in terms of selection from a group.

Should any variable, substituent, or term (e. g. aryl, heterocycle, R, etc.) occur more than one time in a formula or generic structure, its definition at each occurrence is independent of the tion at every other occurrence. Those of skill in the art will further recognize that certain groups may be attached to a parent molecule or may occupy a on in a chain of elements from either end as written. Thus, by way of example only, an unsymmetrical group such as —C(O)N(R)— may be attached to the parent moiety at either the carbon or the nitrogen.

Asymmetric centers exist in the compounds sed herein. These centers are designated by the symbols “R” or “S,” ing on the configuration of substituents around the chiral carbon atom. It should be tood that the invention encompasses all stereochemical isomeric forms, including diastereomeric, enantiomeric, and epimeric forms, as well as d-isomers and l—isomers, and mixtures thereof. Individual stereoisomers of compounds can be prepared synthetically from commercially available ng materials which n chiral centers or by preparation of mixtures of enantiomeric products followed by tion such as conversion to a mixture of diastereomers followed by separation or recrystallization, chromatographic techniques, direct tion of enantiomers on chiral chromatographic columns, or any other appropriate method known in the art. ng nds of particular stereochemistry are either commercially available or can be made and resolved by techniques known in the art. Additionally, the compounds disclosed herein may exist as geometric isomers. The present invention includes all cis, trans, syn, anti, entgegen (E), and zusammen (Z) isomers as well as the appropriate mixtures thereof.

Additionally, compounds may exist as tautomers; all tautomeric s are provided by this invention. Additionally, the compounds disclosed herein can exist in unsolvated as well as solvated forms with pharmaceutically acceptable solvents such as water, ethanol, and the like.

In general, the solvated forms are ered equivalent to the unsolvated forms.

The term “bond” refers to a covalent e between two atoms, or two moieties when the atoms joined by the bond are considered to be part of larger substructure. A bond may be single, , or triple unless otherwise specified. A dashed line between two atoms in a drawing of a molecule indicates that an additional bond may be present or absent at that The term "prodrug" refers to a compound that is made more active in vivo.

Certain compounds disclosed herein may also exist as prodrugs, as described in Hydrolysis in Drug and Prodrug Metabolism .‘ Chemistry, Biochemistry, and Enzymology (Testa, Bernard and Mayer, Joachim M. Wiley-VHCA, Zurich, Switzerland 2003). Prodrugs of the compounds described herein are structurally modified forms of the nd that readily undergo al changes under physiological conditions to e the compound.

Additionally, prodrugs can be converted to the compound by chemical or biochemical methods in an ex vivo environment. For example, prodrugs can be slowly ted to a compound when placed in a transdermal patch reservoir with a suitable enzyme or chemical reagent. Prodrugs are often useful because, in some situations, they may be easier to administer than the nd, or parent drug. They may, for instance, be bioavailable by oral administration whereas the parent drug is not. The prodrug may also have improved solubility in pharmaceutical compositions over the parent drug. A wide variety of prodrug derivatives are known in the art, such as those that rely on hydrolytic ge or oxidative activation of the prodrug. An example, t limitation, of a prodrug would be a compound which is administered as an ester (the "prodrug"), but then is metabolically hydrolyzed to the carboxylic acid, the active entity. Additional examples include peptidyl derivatives of a compound.

The compounds disclosed herein can exist as therapeutically able salts. The present invention es compounds listed above in the form of salts, including acid addition salts. Suitable salts include those formed with both organic and inorganic acids.

Such acid addition salts will normally be pharmaceutically acceptable. r, salts of armaceutically acceptable salts may be of utility in the preparation and cation of the compound in question. Basic addition salts may also be formed and be pharmaceutically acceptable. For a more complete discussion of the preparation and ion of salts, refer to Pharmaceutical Salts: Properties, Selection, and Use (Stahl, P. Heinrich. Wiley-VCHA, Zurich, Switzerland, 2002).

The term “therapeutically acceptable salt,” as used herein, represents salts or zwitten'onic forms of the compounds disclosed herein which are water or oil-soluble or sible and therapeutically acceptable as defined herein. The salts can be prepared during the final isolation and purification of the compounds or separately by ng the appropriate compound in the form of the free base with a suitable acid. Representative acid addition salts include acetate, adipate, alginate, L—ascorbate, ate, benzoate, esulfonate (besylate), bisulfate, butyrate, rate, camphorsulfonate, citrate, digluconate, formate, fumarate, gentisate, glutarate, glycerophosphate, glycolate, lfate, heptanoate, hexanoate, hippurate, hloride, hydrobromide, hydroiodide, 2—hydroxyethansulfonate (isethionate), lactate, maleate, malonate, DL-mandelate, mesitylenesulfonate, methanesulfonate, naphthylenesulfonate, nicotinate, 2-naphthalenesulfonate, oxalate, pamoate, ate, persulfate, 3-phenylproprionate, phosphonate, picrate, pivalate, propionate, pyroglutamate, succinate, sulfonate, tartrate, rate, trichloroacetate, trifluoroacetate, phosphate, glutamate, bicarbonate, para-toluenesulfonate ylate), and undecanoate. Also, basic groups in the compounds sed herein can be quatemized with methyl, ethyl, propyl, and butyl chlorides, bromides, and iodides; dimethyl, diethyl, l, and diamyl sulfates; decyl, lauryl, myristyl, and steryl chlorides, bromides, and iodides; and benzyl and phenethyl bromides. Examples of acids which can be employed to form therapeutically acceptable addition salts include inorganic acids such as hydrochloric, hydrobromic, sulfuric, and phosphoric, and organic acids such as oxalic, maleic, succinic, and citric. Salts can also be formed by coordination of the compounds with an alkali metal or alkaline earth ion. Hence, the present invention contemplates sodium, potassium, magnesium, and calcium salts of the compounds sed herein, and the like.

Basic addition salts can be prepared during the final isolation and cation of the compounds by reacting a carboxy group with a suitable base such as the hydroxide, carbonate, or bicarbonate of a metal cation or with ammonia or an organic primary, ary, or tertiary amine. The cations of therapeutically acceptable salts include lithium, sodium, potassium, calcium, magnesium, and aluminum, as well as nontoxic quaternary amine cations such as ammonium, tetramethylammonium, tetraethylammonium, methylamine, ylamine, hylamine, triethylamine, diethylamine, ethylamine, tributylamine, pyridine, N,N—dimethylaniline, N—methylpiperidine, N—methylmorpholine, dicyclohexylamine, procaine, dibenzylamine, N,N—dibenzylphenethylamine, 1-ephenamine, and N,N‘-dibenzylethylenediamine. Other entative organic amines useful for the formation of base addition salts include ethylenediamine, ethanolamine, diethanolamine, piperidine, and piperazine.

While it may be possible for the compounds disclosed herein to be administered as the raw chemical, it is also possible to present them as a pharmaceutical formulation.

Accordingly, ed herein are pharmaceutical formulations which comprise one or more of certain compounds sed herein, or one or more pharmaceutically acceptable salts, esters, prodrugs, amides, or solvates f, together with one or more pharmaceutically acceptable carriers f and optionally one or more other therapeutic ients. The carrier(s) must be "acceptable" in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient thereof. Proper formulation is dependent upon the route of administration chosen. Any of the well-known techniques, carriers, and excipients may be used as suitable and as understood in the art; e.g., in ton’s Pharmaceutical Sciences. The pharmaceutical compositions disclosed herein may be manufactured in any manner known in the art, e.g., by means of conventional mixing, ving, granulating, -making, levigating, emulsifying, encapsulating, entrapping or compression processes.

The agent — a polyamine analog, polyamine biosynthesis inhibitor, polyamine transport inhibitor, or agent that inhibits SAMDC — may also be administered in combination with one or more entities. In one embodiment, the entity is a therapeutic , including, but not limited to, an anti-viral or anti-retroviral agent, a steroid or other anti-inflammatory agent.

In another embodiment, the entity is a pharmaceutically acceptable carrier.

The optimal dose, frequency of administration, and duration of treatment with the agent in a subject may vary from subject to subject, depending on the disease to be treated or clinical endpoint to be reached (for example, inhibition of infiltration of macrophages to a tissue, or mitigation of pain) the subject's condition, the t's age, weight, response to the treatment, and the nature of the therapeutic entity. Determination of the optimal dose and duration of treatment is within the scope of one of skill in the art. The l dose and duration of treatment may be best determined by monitoring the subj ect's se during the course of the treatment. In some instances, the administration of higher doses may permit less 2014/010714 frequent administration, and lower doses may require more frequent administration in order to achieve a clinically significant improvement in the subj ect's condition. The agent(s) of the invention may be administered as a single dose or in multiple doses.

Generally, a therapeutically effective dose of the agent in accordance with the present methods will be one or more doses of from about 10 to about 1100 mg/mz. Lower dose regimens include doses of 10—200, 10—100, 10—50 and 20-200 mg/mz. Higher dose ns include 200-400,250-500, 400-600, 500-800 600-1000 and 00 mg/mz. In one embodiment, the dose regimens range from 200-400 mg/mz. In another ment, the dose regimens range from 250-500 mg/mz. In yet r embodiment, the dose regimens range from 600-1 000 mg/mz. In some ments the agent is administered daily, once per week, once every other week, or once per month. In one embodiment, a dose regimen g from 200-400 mg/m2 is administered once a week. In another embodiment, a dose regimen g from 250-500 mg/m2 is administered once every other week.

The doses may be constant over the entire treatment period, or they may increase or se during the course of the treatment. In one embodiment, the agent is administered once a week and starts with the administration of 200 mg/mz, and increases to 300 mg/m2 and 400 mg/m2 in the second and third weeks, respectively. In another embodiment, the agent is administered once every other week and is kept constant for the entire duration of treatment with the administration of 250 mg/mz. The doses of the agent may be administered for at least one week, at least two weeks, at least three weeks, at least four weeks, at least 6 weeks, or even at least 8 weeks. Adjusting the dose of the agent within these ranges for a particular t is well within the skill of the ordinary clinician.

The agent may be administered via any conventional route normally used to administer a medicament including, but not limited to, oral, parenteral (including subcutaneous, intradermal, intramuscular, enous, intraarticular, and intramedullary), intraperitoneal, transmucosal (including nasal), transdermal, rectal and topical (including dermal, buccal, sublingual and intraocular) routes. Intravenous delivery may take place via a bolus injection or via infusion; infusion may be done over a period ranging from less than a minute to several hours to continuously. In certain embodiments, a course of treatment will involve administration by a combination of routes.

For example, the agent may be administered via a ation of intravenous and oral routes for the treatment of pain or another disorder. In one embodiment, a “loading” dose may be administered IV in order to bring the concentration of drug to the desired therapeutic level, followed by one or more maintenance doses via the oral route to keep it there. In a further embodiment, a combination of oral and IV delivery may be used to mitigate pain in a surgery patient. The agent may be red pre-, peri-, and post- surgically by a combination of IV and oral routes. In one embodiment, the patient may be administered or may dminister the drug orally prior to y, be administered the drug via IV infusion during surgery and just after, and may thereafter be administered or may self- administer the drug orally after surgery. In another ment, the patient may be administered the drug IV prior to surgery, be administered the drug via IV infusion during y and just after, and may thereafter be administered or may self-administer the drug orally after surgery.

The agent may be administered as a pharmaceutical composition in a variety of forms including, but not limited to, , powder, suspensions, tablets, pills, capsules, sprays and aerosols. The pharmaceutical compositions may include various pharmaceutically able additives including, but not limited to, carriers, excipients, binders, stabilizers, antimicrobial agents, antioxidants, diluents and/or supports. Examples of suitable excipients and rs are described, for example, in “Remington's Pharmaceutical Sciences,” Mack Pub. Co., New Jersey . In some embodiments, the agent may be administered via an IV infusion in an aqueous sugar solution. The agent may also be associated with another substance that facilitates agent delivery. For example, the agent may be associated into liposomes. The liposomes, in turn, may be conjugated with targeting substance(s), such as IgGFc receptors.

Formulations of the compounds disclosed herein suitable for oral administration may be presented as discrete units such as capsules, cachets or tablets each ning a predetermined amount of the active ingredient; as a powder or granules; as a solution or a suspension in an aqueous liquid or a non-aqueous liquid; or as an oil-in-water liquid emulsion or a water-in-oil liquid emulsion. The active ingredient may also be presented as a bolus, electuary or paste. ceutical preparations which can be used orally e tablets, push-fit capsules made of gelatin, as well as soft, sealed capsules made of gelatin and a plasticizer, such as glycerol or sorbitol. Tablets may be made by compression or molding, optionally with one or more accessory ingredients. Compressed tablets may be prepared by compressing in a le machine the active ingredient in a free-flowing form such as a powder or granules, optionally mixed with binders, inert diluents, or lubricating, surface active or dispersing agents. Molded tablets may be made by molding in a suitable machine a e of the powdered compound moistened with an inert liquid diluent. The tablets may optionally be coated or scored and may be formulated so as to provide slow or controlled release of the active ingredient n. All formulations for oral administration should be in s suitable for such administration. The push-fit capsules can contain the active ingredients in admixture with filler such as lactose, binders such as starches, and/or lubricants such as talc or magnesium stearate and, optionally, stabilizers. In soft capsules, the active compounds may be dissolved or suspended in suitable s, such as fatty oils, liquid paraffin, or liquid polyethylene glycols. In addition, stabilizers may be added. Dragee cores are provided with suitable coatings. For this purpose, concentrated sugar solutions may be used, which may ally contain gum arabic, talc, polyvinyl idone, carbopol gel, polyethylene glycol, and/or titanium dioxide, lacquer solutions, and le organic solvents or solvent es. Dyestuffs or pigments may be added to the tablets or dragee coatings for identification or to characterize different combinations of active compound doses.

The compounds may be formulated for eral administration by injection, e. g., by bolus ion or continuous infusion. Formulations for injection may be ted in unit dosage form, 6. g., in ampoules or in multi-dose containers, with an added preservative.

The compositions may take such forms as suspensions, solutions or emulsions in oily or aqueous vehicles, and may n formulatory agents such as suspending, stabilizing and/or dispersing . The formulations may be presented in unit-dose or multi-dose containers, for example sealed ampoules and vials, and may be stored in powder form or in a freeze- dried ilized) condition requiring only the addition of the sterile liquid carrier, for example, saline or sterile pyrogen-free water, immediately prior to use. Extemporaneous injection solutions and suspensions may be prepared from sterile powders, granules and s of the kind previously described.

Formulations for parenteral administration include aqueous and non-aqueous (oily) e injection solutions of the active compounds which may contain antioxidants, buffers, bacteriostats and solutes which render the formulation isotonic with the blood of the intended recipient; and aqueous and non—aqueous sterile suspensions which may include suspending agents and thickening agents. Suitable lipophilic solvents or vehicles include fatty oils such as sesame oil, or synthetic fatty acid esters, such as ethyl oleate or triglycerides, or mes. Aqueous injection suspensions may contain substances which increase the viscosity of the suspension, such as sodium carboxymethyl cellulose, sorbitol, or dextran. Optionally, the suspension may also contain suitable stabilizers or agents which se the solubility of the compounds to allow for the preparation of highly concentrated solutions. 2014/010714 In addition to the formulations described previously, the compounds may also be formulated as a depot preparation. Such long acting formulations may be administered by implantation (for example subcutaneously or intramuscularly) or by intramuscular injection.

Thus, for example, the compounds may be formulated with suitable polymeric or hydrophobic als (for example as an emulsion in an acceptable oil) or ion ge resins, or as sparingly e derivatives, for example, as a sparingly soluble salt.

For buccal or sublingual administration, the compositions may take the form of tablets, lozenges, pastilles, or gels formulated in conventional manner. Such compositions may comprise the active ingredient in a flavored basis such as sucrose and acacia or tragacanth.

The compounds may also be formulated in rectal compositions such as suppositories or retention enemas, e. g., containing conventional suppository bases such as cocoa butter, polyethylene glycol, or other ides.

Certain nds disclosed herein may be administered topically, that is by non- systemic administration. This includes the application of a compound disclosed herein externally to the epidermis or the buccal cavity and the instillation of such a compound into the ear, eye and nose, such that the compound does not significantly enter the blood stream.

In contrast, systemic administration refers to oral, intravenous, eritoneal and intramuscular administration.

Formulations suitable for topical administration include liquid or semi-liquid preparations le for penetration through the skin to the site of inflammation such as gels, liniments, lotions, , ointments or pastes, and drops suitable for administration to the eye, ear or nose. The active ingredient for topical administration may comprise, for example, from 0.001% to 10% w/w (by weight) of the formulation. In certain embodiments, the active ingredient may comprise as much as 10% w/w. In other ments, it may comprise less than 5% w/w. In certain embodiments, the active ingredient may comprise from 2% w/w to % w/w. In other embodiments, it may comprise from 0.1% to 1% w/w of the formulation.

For administration by inhalation, nds may be conveniently delivered from an insufflator, nebulizer pressurized packs or other ient means of delivering an aerosol spray. Pressurized packs may comprise a le propellant such as dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas. In the case of a pressurized l, the dosage unit may be determined by providing a valve to deliver a metered amount. Alternatively, for administration by inhalation or insufflation, the compounds according to the invention may take the form of a dry powder composition, for example a powder mix of the compound and a suitable powder base such as lactose or starch. The powder composition may be presented in unit dosage form, in for example, capsules, cartridges, gelatin or blister packs from which the powder may be administered with the aid of an inhalator or insufflator.

Exemplary unit dosage formulations are those containing an effective dose, as herein below recited, or an appropriate fraction thereof, of the active ingredient.

Fillers to be used in the compositions herein include all those now known and in use, as well as those developed in the future. Examples of fillers, or diluents, e, without limitation, lactose, mannitol, xylitol, dextrose, sucrose, sorbitol, compressible sugar, microcrystalline cellulose (MCC), powdered cellulose, arch, pregelatinized starch, dextrates, n, dextrin, dextrose, extrin, m carbonate, dibasic calcium phosphate, tribasic calcium phosphate, calcium sulfate, magnesium carbonate, ium oxide, poloxamers such as polyethylene oxide, and ypropyl methyl cellulose. Fillers may have complexed solvent molecules, such as in the case where the lactose used is lactose monohydrate. Fillers may also be etary, such in the case of the filler PROSOLV® (available from JRS Pharma). PROSOLV® is a proprietary, optionally high-density, silicified rystalline ose composed of 98% microcrystalline cellulose and 2% colloidal silicon dioxide. Silicification of the microcrystalline cellulose is achieved by a patented process, resulting in an intimate association between the colloidal silicon dioxide and microcrystalline cellulose. ProSolv comes in different grades based on particle size, and is a white or almost white, fine or granular powder, practically insoluble in water, acetone, ethanol, toluene and dilute acids and in a 50g/l solution of sodium hydroxide.

Disintegrants to be used in the compositions herein e all those now known and in use, as well as those developed in the future. Examples of disintegrants include, without tion, sodium starch glycolate, sodium ymethyl ose, calcium carboxymethyl cellulose, croscarmellose sodium, povidone, vidone (polyvinylpolypyrrolidone), methyl cellulose, microcrystalline cellulose, powdered cellulose, low-substituted hydroxy propyl cellulose, starch, pregelatinized starch, and sodium alginate.

Lubricants to be used in the compositions herein include all those now known and in use, as well as those developed in the future. Examples of lubricants include, without limitation, calcium stearate, glyceryl monostearate, glyceryl palmitostearate, hydrogenated vegetable oil, light mineral oil, magnesium stearate, mineral oil, polyethylene , sodium benzoate, sodium lauryl e, sodium stearyl te, stearic acid, talc, and zinc stearate.

Glidants to be used in the compositions herein include all those now known and in use, as well as those developed in the future. Examples of glidants include, without tion, silicon dioxide (SiOz), talc cornstarch, and poloxamers. Poloxamers (or LUTROL®, ble from the BASF Corporation) are A-B—A block copolymers in which the A segment is a hydrophilic polyethylene glycol homopolymer and the B segment is hydrophobic polypropylene glycol homopolymer.

Tablet binders to be used in the compositions herein e all those now known and in use, as well as those developed in the future. Examples of tablet binders include, t tion, acacia, alginic acid, er, carboxymethyl cellulose sodium, dextrin, ellulose, gelatin, guar gum, hydrogenated vegetable oil, hydroxyethylcellulose, hydroxypropyl cellulose, hydroxypropylmethyl cellulose, copolyvidone, methyl cellulose, liquid glucose, maltodextrin, thacrylates, povidone, atinized starch, sodium alginate, starch, sucrose, tragacanth, and zein.

Examples of surfactants include, without limitation, fatty acid and alkyl sulfonates; commercial surfactants such as benzethanium chloride (HYAMINE® 1622, available from Lonza, Inc., Fairlawn, N.J.); DOCUSATE SODIUM® (available from Mallinckrodt Spec. Chem, St. Louis, MO); polyoxyethylene sorbitan fatty acid esters (TWEEN®, available from ICI Americas Inc., Wilmington, DE; RB® P-20, available from Lipochem Inc., Patterson NJ; CAPMUL® POE-0, available from Abitec Corp., ille, WI), polyoxyethylene (20) sorbitan monooleate (TWEEN 80®, available from ICI Americas Inc., Wilmington, DE); and natural tants such as sodium holic acid, l-palmitoyloleoyl-sn-glycerophosphocholine, lecithin, and other phospholipids and mono— and diglycerides. Such materials can advantageously be employed to increase the rate of dissolution by facilitating wetting, thereby increasing the maximum dissolved concentration, and also to t crystallization or precipitation of drug by interacting with the dissolved drug by mechanisms such as complexation, formation of inclusion complexes, formation of micelles or adsorbing to the surface of solid drug Drug complexing agents and lizers to be used in the compositions herein include all those now known and in use, as well as those developed in the future. Examples of drug xing agents or solubilizers include, without limitation, the polyethylene glycols, caffeine, xanthene, gentisic acid and cylodextrins.

The addition of pH modifiers such as acids, bases, or buffers may also be beneficial, retarding or enhancing the rate of dissolution of the composition, or, alternatively, helping to improve the al stability of the composition. Suitable pH modifiers to be used in the compositions herein include all those now known and in use, as well as those developed in the future.

It should be understood that in addition to the ingredients ularly mentioned above, the formulations provided herein may include other agents conventional in the art having regard to the type of formulation in question. Proper formulation is dependent upon the route of administration chosen. Any of the nown techniques, carriers, and ents may be used as suitable and as understood in the art; e. g., Remington, supra. The pharmaceutical compositions may be manufactured in a manner that is itself known, e.g., by means of conventional mixing, dissolving, ating, dragee-making, levigating, emulsifying, encapsulating, entrapping or compression ses. nds may be administered orally or via injection at a dose of from 0.1 to 500 mg/kg per day. The dose range for adult humans is generally from 5 mg to 2 g/day.

Tablets or other forms of presentation provided in discrete units may conveniently contain an amount of one or more compounds which is effective at such dosage or as a le of the same, for instance, units containing 5 mg to 500 mg, usually around 10 mg to 200 mg.

The precise amount of compound stered to a subject will be the responsibility of the attendant physician. The specific dose level for any particular subject will depend upon a variety of factors ing the activity of the specific compound employed, the age, body weight, general , sex, diets, time of administration, route of administration, rate of ion, drug combination, the precise disorder being treated, and the severity of the indication or condition being treated. Also, the route of administration may vary depending on the condition and its severity. Dosing frequency may also be selected or adjusted based on factors including those above as well as the formulation of the compound delivered. Dosing may occur, for example: once daily, twice daily, three or four times daily, every other day, weekly, bi-weekly, or monthly; or in cycles comprising a sustained dosing period followed by a non-dosing period; or on an as-needed basis.

In certain instances, it may be appropriate to administer at least one of the compounds described herein (or a pharmaceutically acceptable salt, ester, or prodrug thereof) in combination with another eutic agent. By way of example only, if one of the side effects experienced by a subject upon receiving one of the compounds herein is hypertension, then it may be appropriate to administer an anti-hypertensive agent in combination with the initial therapeutic agent. Or, by way of example only, the therapeutic effectiveness of one of the compounds described herein may be ed by administration of an adjuvant (i.e., by itself the adjuvant may only have minimal eutic benefit, but in combination with another therapeutic agent, the overall therapeutic benefit to the subject is enhanced). Or, by way of example only, the benefit enced by a subject may be increased by stering one of the compounds described herein with another therapeutic agent (which also includes a therapeutic regimen) that also has therapeutic benefit. By way of example only, in a treatment for neuropathy involving administration of one of the nds described herein, increased therapeutic benefit may result by also providing the subject with another therapeutic agent for neuropathy. In any case, regardless of the disease, disorder or condition being d, the overall t experienced by the subject may simply be additive of the two therapeutic agents or the subject may experience a synergistic benefit.

In certain embodiments, the other eutic agent is an agent for the treatment of multiple sclerosis such as interferon beta-la, interferon beta-lb, glatiramer acetate, mitoxantrone, natalizumab, fingolimod, dimethyl fumarate dera) and teriflunomide, as well as other interferons and immunomodulatory, immunosuppressant, or anti-inflammatory drugs.

In other embodiments, the other therapeutic agent is a TNF inhibitor. The TNF inhibitor may be: a monoclonal dy such as, for example, infliximab (Remicade), adalimumab (Humira), certolizumab pegol a), or golimumab (Simponi); a circulating receptor fusion protein such as etanercept (Enbrel); or a small molecule, such as pentoxifylline or bupropion (Zyban, Wellbutrin).

In other embodiments, the other therapeutic agent is a e-modifying anti- rheumatic drug ). Examples of DMARDs include azathioprine, ciclosporin (cyclosporine A), D-penicillamine, gold salts, hydroxychloroquine, leflunomide, methotrexate (MTX), cline, sulfasalazine ($82), and cyclophosphamide.

In further embodiments, the other therapeutic agent is methotrexate.

Other agents for used in combination include interleukin 1 (IL-1) blockers such as anakinra (Kineret), T-cell costimulation blockers such as abatacept (Orencia), interleukin 6 (IL-6) rs such as tocilizumab (an anti-IL-6 receptor antibody; RoActemra, a), monoclonal antibodies against B cells such as rituximab (Rituxan), and other ics (eg.

Ocrelizumab, Ofatumumab, Golimumab, and izumab pegol).

In other embodiments, the other therapeutic agent is a glucocorticoid or a non- steroidal anti-inflammatory drug (NSAID). NSAIDS e propionic acid derivatives such as ibuprofen, naproxen, fenoprofen, ketoprofen, rofen, and oxaprozin; acetic acid derivatives such as indomethacin, sulindac, etodolac, and diclofenac; enolic acid (oxicam) derivatives such as piroxicam and meloxicam; fenamic acid derivatives such as mefenamic acid and meclofenamic acid; selective COX-2 tors (Coxibs) such as celecoxib (Celebrex), rofecoxib, oxib, parecoxib, lumiracoxib, and etoricoxib.

In any case, the multiple therapeutic agents (at least one of which is a nd disclosed herein) may be administered in any order or even simultaneously. If simultaneously, the multiple therapeutic agents may be provided in a single, unified form, or in multiple forms (by way of example only, either as a single pill or as two separate pills).

One of the therapeutic agents may be given in multiple doses, or both may be given as multiple doses. If not simultaneous, the timing between the doses of the le therapeutic agents may be any duration of time ranging from a few minutes to four weeks.

Thus, in another aspect, certain embodiments provide methods for treating disorders in a human or animal subject in need of such treatment comprising administering to said subject an amount of a compound disclosed herein effective to reduce or prevent said disorder in the t, optionally in combination with at least one additional agent for the treatment of said disorder that is known in the art. Specific diseases to be treated by the compounds, compositions, and methods disclosed herein, singly or in combination, include, without limitation: pain; neuropathy; inflammation and related ers; arthritis; lic inflammatory disorders; respiratory disorders; autoimmune ers; ogical disorders; and proliferative disorders, including cancer and non-cancerous es.

The nds disclosed herein are useful to treat patients with pain, including neuropathy and/or neuropathic pain, and inflammatory pain. Pain indications include, but are not limited to, treatment or prophylaxis of surgical or post-surgical pain for s surgical procedures including amputation, post-cardiac surgery, dental pain/dental extraction, pain resulting from cancer, muscular pain, mastalgia, pain resulting from dermal injuries, lower back pain, headaches of various etiologies, including ne, menstrual cramps, and the like. The compounds are also useful for the treatment of pain-related disorders such as tactile allodynia and hyperalgesia. The pain may be somatogenic (either nociceptive or neuropathic), acute and/or chronic.

Peripheral neuropathies which can be treated with the compounds disclosed herein include mono-neuropathies, mono-multiplex neuropathies, and europathies, including axonal and demyelinating neuropathies. Both sensory and motor neuropathies are encompassed.

The neuropathy or neuropathic pain may be associated with a number of peripheral neuropathies of varying etiologies, ing but not limited to: 0 trauma-induced neuropathies, including those caused by physical injury (such as blunt , abrasion, or burns) or disease state, physical damage to the brain, physical WO 10154 damage to the spinal cord, or stroke associated with brain damage; neurological disorders related to neurodegeneration; and urgical athies and neuropathic pain (such as from tumor resection, mastectomy, and the like) 0 infectious and Viral neuropathies, including those caused by leprosy, Lyme disease, a herpes Virus (and more particularly by a herpes zoster Virus, which may lead to postherpetic neuralgia), human immunodeficiency Virus (HIV, which may lead to HIV neuropathy), or a papilloma Virus, or any other pathogen-induced nerve damage; 0 induced neuropathies (including but not limited to neuropathies induced by alcoholism, Vitamin B6 intoxication, hexacarbon intoxication, amiodarone, chloramphenicol, disulfiram, isoniazide, gold, lithium, metronidazole, misonidazole, nitrofurantoin) ; 0 drug-induced neuropathies, including therapeutic-drug-induced neuropathy, particularly a) chemotherapy-induced athies caused by anti-cancer agents such as taxol, taxotere, cisplatin, nocodazole, Vincristine, Vindesine and stine, and b) iral neuropathies caused by anti-Viral agents such as ddI, DDC, d4T, foscarnet, dapsone, metronidazole, and zid); 0 Vitamin-deficiency—induced neuropathies including those resulting from Vitamin B 12 deficiency, Vitamin B6 deficiency, and Vitamin E deficiency); 0 hereditary neuropathy (including but not limited to Friedreich ataxia, familial amyloid polyneuropathy, Tangier disease, Fabry disease; 0 diabetic neuropathy and neuropathy caused by metabolic disorders such as renal insufficiency and hypothyroidism; 0 neuropathy secondary to tumor infiltration, 0 auto-immune neuropathies, including those resulting from Guillain-B arre syndrome, c inflammatory de-myelinating polyneuropathy, monoclonal gammopathy of undetermined significance and polyneuropathy, and le sclerosis; 0 other neuropathies and neuropathic pain syndromes including inflammation—induced nerve damage, neurodegeneration, post-traumatic neuralgia, central neuropathic pain syndromes such as phantom limb pain, pain, x regional pain syndromes (including but not limited to reflex sympathetic dystrophy, causalgia), neoplasia- associated pain, vasculitic/angiopathic neuropathy, and ca; and 0 idiopathic neuropathies.

In n embodiments, neuropathic pain may alternatively be manifested as allodynia, hyperalgesic pain, thermal hyperalgesia, or phantom pain. In another embodiment, neuropathy may instead lead to loss of pain sensitivity. Additional sub-categories of neuropathic pain are discussed in Dworkin, Clin J Pain (2002) vol. 18(6) pp. 343-9.

Compounds sed herein can also be used in the treatment or prevention of opiate tolerance in patients needing cted opiate analgesics, and benzodiazepine tolerance in patients taking benzodiazepines, and other addictive behavior, for example, nicotine addiction, alcoholism, and eating disorders. Moreover, the compounds sed herein are useful in the ent or prevention of drug withdrawal symptoms, for example treatment or tion of symptoms of withdrawal from opiate, alcohol, or tobacco addiction.

Compounds sed herein can also be used in the treatment or prevention of respiratory e or conditions, including: asthmatic conditions including allergen—induced asthma, exercise—induced asthma, pollution-induced asthma, cold-induced asthma, and viral- induced-asthma; c obstructive pulmonary diseases including chronic bronchitis with normal airflow, c bronchitis with airway obstruction (chronic ctive bronchitis), emphysema, tic bronchitis, and s disease; and other pulmonary diseases involving ation including bronchioectasis cystic fibrosis, hypersensitivity pneumonitis, farmer's lung, acute respiratory distress syndrome, pneumonia, aspiration or inhalation injury, fat embolism in the lung, acidosis inflammation of the lung, acute pulmonary edema, acute mountain sickness, acute pulmonary hypertension, persistent pulmonary hypertension of the newborn, perinatal aspiration syndrome, hyaline membrane disease, acute pulmonary thromboembolism, heparin-protamine reactions, sepsis, status asthamticus, hypoxia, hyperoxic lung injuries, and injury induced by inhalation of certain injurious agents including cigarette smoking, leading up to complications thereof such as lung carcinoma.

Compounds disclosed herein can also be used in the treatment or prevention of inflammation and atory conditions. Inflammatory conditions include, without limitation: arthritis, including sub—types and related conditions such as rheumatoid tis, spondyloarthropathies, gouty arthritis, osteoarthritis, ic lupus erythematosus, juvenile arthritis, acute rheumatic arthritis, enteropathic arthritis, neuropathic arthritis, psoriatic arthritis, and ic tis; osteoporosis, tendonitis, bursitis, and other related bone and joint disorders; gastrointestinal conditions such as reflux esophagitis, diarrhea, inflammatory bowel disease, Crohn's disease, gastritis, irritable bowel syndrome, ulcerative colitis, acute and chronic inflammation of the pancreas; pulmonary inflammation, such as that associated with viral infections and cystic fibrosis; skin-related conditions such as psoriasis, eczema, burns, sunburn, dermatitis (such as contact dermatitis, atopic dermatitis, and allergic dermatitis), and hives; pancreatitis, hepatitis, pruritis and vitiligo. In addition, nds of invention are also useful in organ transplant patients either alone or in combination with conventional immunomodulators.

Compounds disclosed herein can also be used in the treatment or prevention of autoimmune disorders. Autoimmune disorders include Crohns disease, tive s, dermatitis, dermatomyositis, es us type 1, Goodpasture's syndrome, Graves’ disease, in-Barré syndrome (GBS), autoimmune encephalomyelitis, Hashimoto‘s disease, idiopathic ocytopenic purpura, lupus erythematosus, mixed connective tissue disease, multiple sclerosis (MS), myasthenia gravis, narcolepsy, pemphigus vulgaris, pernicious anemia, psoriasis, tic arthritis, polymyositis, primary biliary cirrhosis, rheumatoid arthritis, n‘s syndrome, derma, temporal arteritis (also known as "giant cell arteritis"), vasculitis, and Wegener's granulomatosis. The compounds disclosed herein may regulate TH-17 (T-helper cells producing interleukin 1?) cells or IL-17 .

Autoimmune disorders may affect the nervous system. Examples of autoimmune disorders affecting the nervous system include polymyalgia, enia gravis, Guillain- Barré Syndrome, chronic atory demyelinating polyneuropathy, transverse myelitis, Balo tric sclerosis, pernicious anemia, acute disseminated encephalomyelitis (ADME), amyotrophic lateral sis (ALS), autoimmune peripheral neuropathy, lupus erythematosus, psoriatic arthritis, rheumatoid arthritis, osteoarthritis, and tic fever.

Compounds disclosed herein can also be used in the ent or prevention of demyelinating es, including multiple sclerosis (MS), optic neuritis, idiopathic inflammatory demyelinating diseases, Guillain-Barré Syndrome (and pes), chronic inflammatory demyelinating polyneuropathy, transverse myelitis, Balo concentric sclerosis, pernicious anemia, central pontine myelinolysis, Tabes dorsalis, neuromyelitis optica (NMO), progressive multifocal leukoencephalopathy (PML), anti-MAG (myelin-associated glycoprotein) neuropathy, hereditary motor and sensory neuropathy (Chacot—Marie—Tooth disease), cerebrotendinious xanthanomatosis, and leukodystrophies including adrenoleukodystrophy, adrenomyeloneuropathy, metachromatic leukodystrophy, globoid cell leukodystrophy (Krabbe disease), Canavan disease, vanishing white matter disease, Alexander disease, Refsum e, and Pelizaeus—Merzbacher disease.

Compounds disclosed herein can also be used in the treatment or prevention of certain diseases and ers of the s . Central nervous system disorders in which nitric oxide inhibition is useful include cortical dementias including Alzheimer‘s disease, central nervous system damage resulting from stroke, ischemias including cerebral ischemia (both focal ischemia, thrombotic stroke and global ischemia (for example, secondary to c arrest), and trauma. Neurodegenerative disorders in which nitric oxide inhibition is useful include nerve degeneration or nerve is in disorders such as hypoxia, hypoglycemia, epilepsy, and in cases of central nervous system (CNS) trauma (such as spinal cord and head injury), hyperbaric oxygen-induced convulsions and toxicity, dementia e.g. nile dementia, and AIDS-related dementia, cachexia, am's chorea, Huntington's e, Parkinson’s Disease, amyotrophic lateral sclerosis (ALS), Korsakoffs disease, cognitive disorders relating to a cerebral vessel disorder, hypersensitivity, sleeping disorders, schizophrenia, depression, depression or other symptoms associated with Premenstrual Syndrome (PMS), and anxiety.

Compounds disclosed herein can also be used in the treatment or prevention of metabolic disorders that are typically associated with an exaggerated inflammatory signaling, such as insulin resistance, diabetes (type I or type II), metabolic syndrome, nonalcoholic steatohepatitis, atherosclerosis, cardiovascular disease, congestive heart failure, myocarditis, atherosclerosis, and aortic aneurysm.

Compounds disclosed herein can also be used in the treatment or prevention of organ and tissue injury associated with severe burns, sepsis, trauma, , and hemorrhage- or resuscitation-induced hypotension, and also in such diseases as vascular diseases, migraine headaches, periarten'tis nodosa, thyroiditis, aplastic anemia, Hodgkin's disease, doma, rheumatic fever, type I diabetes, neuromuscular junction disease ing myasthenia gravis, white matter disease including le sclerosis, sarcoidosis, tis, tic syndrome, 's syndrome, polymyositis, gingivitis, periodontis, swelling occurring after injury, ischemias including myocardial ia, cardiovascular ischemia, and ischemia secondary to cardiac arrest, and the like.

Compounds disclosed herein can also be used in the ent or tion of (hyper) proliferative diseases, especially cancers, either alone or in combination of rds of care especially those agents that target tumor growth by re-instating the aberrant apoptotic machinery in the malignant cells. Hematological and non-hematological malignancies which may be treated or prevented include but are not d to multiple myeloma, acute and chronic leukemias including Acute Lymphocytic Leukemia (ALL), Chronic Lymphocytic Leukemia (CLL), and Chronic enous Leukemia(CLL), lymphomas, including n’s lymphoma and non-Hodgkin’s lymphoma (low, intermediate, and high grade), as well as solid tumors and malignancies of the brain, head and neck, breast, lung, reproductive tract, upper ive tract, pancreas, liver, renal, bladder, prostate and colorectal. The present compounds and methods can also be used to treat the fibrosis, such as that which occurs with radiation therapy. The present compounds and methods can be used to treat subjects having adenomatous polyps, including those with al adenomatous polyposis (FAP). Additionally, the present compounds and methods can be used to prevent polyps from forming in patients at risk of PAP. Non-cancerous proliferative ers additionally include psoriasis, eczema, and dermatitis.

Compounds disclosed herein can also be used in the treatment or tion of polycystic kidney disease, as well as other diseases of renal dysfunction.

Compounds sed herein can also be used in the treatment or prevention of ophthalmic diseases, such as glaucoma, retinal on degeneration, ocular ischemia, corneal neovascularization, optic neuritis, retinitis, retinopathies such as glaucomatous retinopathy and/or diabetic retinopathy, uveitis, ocular photophobia, dry eye, Sj ogren‘s syndrome, seasonal and chronic ic conjunctivitis, and of inflammation and pain associated with chronic ocular disorders and acute injury to the eye tissue. The compounds can also be used to treat post-operative inflammation or pain as from ophthalmic surgery such as cataract surgery and refractive surgery.

The present compounds may also be used in co-therapies, partially or completely, in place of other conventional anti-inflammatory therapies, such as together with steroids, NSAIDs, COX-2 selective tors, xygenase inhibitors, LTB4 antagonists and LTA4 hydrolase inhibitors. The compounds of the subject ion may also be used to prevent tissue damage when therapeutically combined with antibacterial or ral agents.

Exemplary embodiments of the present methods are provided in the following examples. The following examples are presented to illustrate the methods of the invention and to assist one of ordinary skill in using the same, and are not to be construed as limiting the scope of the invention.

MGBG Oral Activity Assays The following standard abbreviations are used to represent the ated pharmacokinetic parameters.

AUC Area under the curve up to the last measurable concentration plus the AUC extrapolated from the last measurable concentration (Clem at tlast) to infinity: AUCINFobS = AUleast + Clam/Lambda z (where ltz is the first order rate constant associated with the terminal (log-linear) portion of the curve) AUC0_12 Area under the curve between the time of dose and the 12 h time point AUC0.24 Area under the curve between the time of dose and the 24 h time point F Fraction ble (bioavailability): F = [AUCoral]' doseiV / [AUCiv]' doseoral C101,S Observed clearance VssobS Steady state volume of distribution Vd Volume of bution (often used with oral) bS Apparent total body clearance as a function of bioavailability tm Terminal half-life (HLM) Cmax The m observed concentration Tmax The time at which Cmax occurred Rhesus Macaque Single-Dose Two groups of three male rhesus monkeys were fasted overnight before being administered the test article, MGBG, as either a single bolus intravenous dose of 1 mg/kg (Group 1) or as a single oral gavage dose of 10 mg/kg (Group 2). Dose formulation analysis verified administered dose solutions as within 14% of targeted concentrations of 1 and 10 mg/kg for Groups 1 and 2, tively.

Blood samples were ted into tubes containing lithium heparin from the femoral vein/artery (approximately 1.0 mL) for plasma MGBG concentration measurement from all intravenously dosed animals prior to dosing and at approximately T=0.083 (5 min), 0.25 (15 min), 0.5 (30 min), 1, 2, 4, 8, and 24 hours after dosing. Blood samples for plasma MGBG concentration ement were collected from all orally dosed animals prior to dosing at approximately T=1, 2, 4, 8, 12, 24, and 36 hours after dosing. Food was also withheld through the first four hours of blood sample tion.

The samples were centrifuged under refrigerated conditions ing completion of sample collection at each interval. The resulting plasma was separated and stored frozen at approximately -70°C until analysis.

PK analysis was performed on the individual plasma tration-time profiles for MGBG using the WinNonlin non-compartmental approach (linear trapezoidal rule for AUC ations). Nominal dose values and sampling times were used for calculations. All MGBG plasma concentration measurements reported as BQL (< 2.51 ng/mL) were set equal to zero for the purpose of analysis. Following IV and PO administration of MGBG, plasma PK disposition parameters were ated using the WinNonlin default selection ia for the selection of the Lambda Z.

Evidence of systemic plasma MGBG exposure was observed at all collected plasma time points following IV and PO administration of MGBG. Hemolysis was noted in one animal in Group 1 at a single time point, which may have negatively ed the MGBG plasma concentration analysis for this animal. Consequently, a model-dependent twocompartmental analysis was used to calculate bioavailability.

Dog Single-Dose Two groups of three male beagle dogs weighing 9.0-105l kg and aged 8-30 months were fasted overnight before being administered the test article, MGBG, as either a single bolus intravenous dose of 1 mg/kg (Group 1) or as a single oral gavage dose of 10 mg/kg (Group 2). Dose formulation analysis verified administered dose solutions as within 17% of targeted concentrations of l and 10 mg/kg for Groups 1 and 2, tively.

Blood samples ximately 2.0 mL) were collected for plasma MGBG concentration measurement from all intravenously dosed animals prior to dosing and at approximately T=0.083 (5 min), 0.25 (15 min), 0.5 (30 min), 1, 2, 4, 8, and 24 hours after . A similar procedure was used with orally dosed animals was used, except that collection took place at T=l, 2, 4, 8, 12, 24, and 36 hours after dosing. The samples were centrifuged under refrigerated conditions following completion of sample collection at each interval. The resulting plasma was separated and stored frozen at approximately -70°C until analysis.

Analysis was performed by LC/MS/MS, and plasma PK disposition parameters were calculated using the last five plasma concentrations for IV (l—24 h) and PO (4—36 h) administration for the selection of the Lambda Z. Due to inter-animal variability and limited terminal phase data, these s should be interpreted with caution. [05 34] No clinically abnormal findings followed IV or oral administration. Systemic exposure was observed at all time points.

Rat -Dose Eighteen male Sprague Dawley rats (Charles River) weighing 3 g and aged 8-9 weeks were administered the test e, MGBG as either a single bolus intravenous dose of 1 mg/kg (Group 1) or as a single oral gavage dose of 10 mg/kg. A cohort of three animals was sacrificed via C02 inhalation anesthesia after final blood collection at each of T = 2, 4, 12, 24, 36, and 48 hours post-dose. Dose formulation analysis verified administered dose solutions as within 17% of ed concentration of 10 mg/kg.

Analysis was performed by LC/MS/MS. Pharmacokinetic analyses were performed on the mean MGBG plasma concentration versus time data using the non- compartmental approach (linear trapezoidal rule for AUC ations). The WinNonlin sparse sampling tool was used for PK calculations. All samples reported as BLQ (Below the Limit of Quantitation, in plasma 2.50 ng/mL) were changed to 0.00 ng/mL for the purpose of analysis. Dose formulation analysis revealed that formulations were within 15% of the targeted dose concentration of 10 mg/kg. al clinical findings were not noted ing dosing. A single PO administration of 10 mg/kg of MGBG resulted in evidence of measurable MGBG levels in plasma through the 12 hour time point; beyond that point, certain samples began e BLQ. onally, in rats dosed as above with single oral administration of MGBG at mg/kg, immediately following the plasma sample collection for each cohort (i.e., 2, 4, 12, 24, 36, and 48 hours after dosing), three rats were sacrificed, and spleen and liver tissues collected and flask frozen. As shown on , a single oral administration of MGBG at 10 mg/kg resulted in greater overall exposure (as assessed by Cmax and AUCan) Of MGBG to liver (120- and 160-fold, respectively) and spleen (4.0- and 9.3-fold, respectively) tissue compared to plasma. This is consistent with a selective uptake mechanism for MGBG.

Without g to be bound by theory, other SAMDC inhibitors which are selectively uptaken by cells may, like MGBG, be useful in the methods and compositions disclosed herein.

Mouse Single-Dose Twenty-four male DBA/1 mice weighing 19.5—24.7 g and aged 7-9 weeks administered the test article, MGBG, as either a single bolus intravenous dose via a lateral tail vein of 1 mg/kg (Group 1, n = 12) or as a single oral gavage dose of 10 mg/kg (Group 2, n = 12). Each dose group consisted of 4 cohorts of 3 animals each. Group 1 was sampled at 5, , and 30 minutes after dosing; and 1, 2, 4, 8, and 24 hours after dosing. Group 2 was d at 1, 2, 4, 8, 12, 24, and 36 hours after dosing. Starting with the first time point, a new cohort was sampled at each sive time point up to the 1-hour (Group 1) or 12-hour (Group 2) time point. The order of sampling among the cohorts was repeated for the subsequent time points (some cohorts may have been bled only once). The second bleed for each cohort was terminal. Animals were sacrificed via C02 inhalation anesthesia after final blood collection.

The samples were centrifuged under refrigerated conditions following completion of sample collection at each interval. The resulting plasma was separated and stored frozen at imately -70°C until analysis. Analysis was performed by LC/MS/MS.

Pharmacokinetic analyses were performed on the mean MGBG plasma concentration versus time data using the non-compartmental approach (linear trapezoidal rule for AUC calculations). The WinNonlin sparse sampling tool was used for PK calculations. Dose ation analysis revealed that the IV and PO formulations were within 15% of their targeted concentrations.

Abnormal clinical findings were not noted following dosing. Evidence of systemic plasma MGBG re was observed at all ted plasma time points following IV and PO administration of MGBG. s of the foregoing assays are shown below in Tables 2 and 3. Values reported are mean across treatment groups without standard deviation.

Table2 Clobs VSSobs IV Tm” (h) (ng/mL) n/kg) (L/kg) (h*ng/mL) ---——-— mouse 0083 496 383 .8 0.083 1180 13.7 .

Table 3 TmaX Cmax AUC Clobs Vd ORAL 111/2 ([1) F % (h) (ng/mL) (h*ng/mL) (mL/min/kg) (L/kg) rhesus 24.2 3.33 192 4240 6.63 13.2 ‘ 35.0% rat 28.1 4.67 55.8 1280** 3.18 15.4 11.6% mouse 11.8 1 106 1420 6.68 38.3 44.3% dog 15.5 1 616 6290 8.29 14.6 49.0% In Table 2 above, the double asterisk indicates that the rat AUC reported is the AUCau, computed from time zero to the time of the last plasma concentration measurement.

Each of these values carries the caveat that terminal measurements are subject to different methods of extrapolation.

MULTI-DOSE RAT PHARMACOKINETIC AND TOLERABILITY STUDY The e of this study was to determine the pharmacokinetic (PK) properties and tolerability of MGBG in rats. Additionally, recovery from any toxic effects was assessed after a seven day non-dosing period. bility was demonstrated in test article-treated animals by body weight changes similar to the control group and a lack of adverse clinical observations.

Three per group of male Sprague Dawley (CD® IGS, Charles River) aged 7—9 weeks and weighing 222.7—252.0 g were administered by oral (PO) gavage, twice daily, at , 20, or 30 mg/kg/dose (20, 40, or 60 mg/kg/day) for seven consecutive days. A washout period of seven days ed. tion of approximately 200 [1L of whole blood was collected from the tail vein of all animals in Groups 5, 6, and 7 were bled at six (Day 1), seven (Day 7), or one (Days 9 through 15) time point(s), respectively. Whole blood samples were collected in a lithium heparin microtainer and processed to plasma by centrifugation.

Plasma was frozen at -70°C. Pharmacokinetic analyses were performed on the individual animal plasma concentration versus time data for MGBG using lin (linear trapezoidal rule for AUC calculations). Nominal dose values and sampling times were used for calculations. For Study Day 7, the reported values for MGBG concentrations at time zero were used in the calculations of AUC. Study Day 1 disposition parameters were not reported due to insufficient terminal phase data to adequately characterize these parameters. Following PO stration of MGBG on Study Day 7, plasma PK disposition parameters were calculated on plasma concentrations obtained ing the second administered dose (T=12— 192 h) using the WinNonlin t selection criteria for the selection of the Lambda Z, the ation rate constant, upon which half-life, AUCINFobS, and Cl/Fobs, were based; animal variability was noted.

Plasma samples collected from test e-treated animals on Day 1 and Day 7 were subjected to bioanalysis and confirmed systemic exposure to the test article at all time points. Over the dose range evaluated, Tmax values were dose-dependent and ranged from 3.33 to 14.0 h, and indicated absorption was slightly delayed on Study Day 7 compared to Study Day 1. Systemic exposure (as assessed by Cmax and AUCau) increased with increasing dose, and the increase in both parameters was slightly less than dose—proportional at each evaluation interval. Repeat, twice—daily PO dosing of MGBG was associated with 3.77-, 403-, and 3.68-fold increases in mean AUCau values compared to Study Day 1 for the 20, 40, and 60 mg/kg/day dose groups, respectively. On Study Day 7, evidence of dose-dependent dispositions for CllFobS and elimination half-life were observed as mean parameter values for Cl/FobS and elimination ife increased and decreased, respectively, with increasing dose levels.

Differences between the control group and the 60 mg/kg/day dose group were noted for a few hematology ters (lower reticulocyte count and percentage) and some serum chemistry parameters (e.g., osmolality and electrolyte changes consistent with slight dehydration). However, these changes were not t to be adverse as they did not coincide with other signs of frank toxicity and the serum chemistry changes were demonstrated to be reversible. No gross or microscopic s were observed in test article— treated animals at terminal sacrifice, and no gross lesions were observed in test article-treated animals at recovery sacrifice.

Based on the findings of this exploratory study, the no observable e effect level ) for MGBG administered by P0 gavage twice daily for seven utive days to male e Dawley rats is 30 mg/kg/dose (60 mg/kg/day).

Table 4 PO Dose Day 1 CmX Tmax AUC (mg/kg/day) (ng/mL) (h) (h*ng/mL) (h*ng/mL) (h*ng/mL) Cl/Fobs 60 296 3.33 NC 2170 4380 NC Table 5 7930 ‘ 1030 2190 13700 ‘ 1800 3690 17100 3130 6040 ALLOMETRIC SCALING AND PREDICTED HUMAN EFFICACY Multi-species allometric scaling based on pharmacokinetic ters disclosed in Tables 2 and 3 was employed to calculate predicted pharmacokinetic parameters in humans according to methods known in the art. See, e.g., Ings RM, “Interspecies scaling and comparisons in drug pment and toxicokinetics,” Xenobiotica, 1990 Nov;20(11):1201— 31 and Khor, SP et al., “Dihydropyrimidine dehydrogenase inactivation and 5-fluorouracil cokinetics: allometric g of animal data, pharmacokinetics and toxicodynamics of —fluorouracil in humans,” Cancer Chemother Pharmacol (1997) 39(3): 833—38. Expected values are given below in Tables 6 and 7.

Table 6 CL Vss IV t1/2 (h) (mL/min/kg) (L/kg) Based on 13.4 7.7 9‘0 Mouse, Rat, Do , Rhesus Based 0“ 13.3 7.9 9.1 Mouse, Dog, Rhesus Table 7 CL Vss ha (11) (mL/min/kg) (L/kg) Based on 21.0 42.4 Mouse, Rat, Do_, Rhesus Based on Mouse, Do_, Rhesus [05 50] In both the murine carrageenan—induced paw edema and hyperalgesia models, the top efficacious dose of MGBG is 30 mg/kg PO BID (totaling 60 mg/kg/day). Based upon this dosing paradigm in mice, at least two s to estimate the equivalent dosing in humans may be used.

The first method is based upon body surface area (BSA) normalization (described in Reagen—Shaw et al. (2007) FASEB J. 22, 659-661), as the authors note that BSA correlates well across species for various biological parameters, including basal metabolic rate, blood , caloric expenditure, plasma protein levels, and renal function. Using this method, a 60 mg/kg/day dose in mice would convert to about 4.9 mg/kg/day in humans.

The second method used to convert the efficacious 60 day dose in mice to an lent dose in humans was based more directly on allometric scaling. Data from an MGBG pharmacokinetic study consisting of a 10 mg/kg oral dose in mice was modeled in a tion to determine the theoretical AUCINF value for a dosing regimen of 30 mg/kg PO BID, which was 9050 h*ng/mL. Next, predicted human clearance values as determined by single- and multi-species allometric g were used to te doses likely to produce an exposure in humans (AUCINF) similar to that of the 60 mgx’kg/day in mice. Using singlespecies allometric g and a range of predicted human clearance values, a human equivalent dose would be in the range of 1.73 mg/kg/day to 4.51 mg/kg/day. Using multi- species allometric scaling, the predicted human equivalent dose is about 4.2 mg/kg/day.

In the murine carrageenan models, we also observed efficacy of MGBG at lower doses, including 3 mg/kg PO BID and 10 mg/kg PO BID, which would proportionally t to human doses of ~0.49 day and ~1.6 mg/kg/day.

The average body weight of a normal male human is often presumed to be 70 kg.

Thus, daily doses based on the predictions above could be estimated to range from about 25mg/day to about 350 mg/day.

The proper dose depends, of course, on a number of factors. The patient may weigh much more or much less, or be female, elderly, or juvenile, requiring a lower or higher dose. The patient may exhibit a drug metabolic profile which might counsel for a lower or higher dose, such as a low expression level or ty of metabolizing enzymes such as cytochromes P450 (CYPs). This low expression or activity level may be due to a number of factors. Polymorphic expression of one or more CYPs (for example CYP2C19 and CYP2D6, though rphisms have been described for nearly all the CYPs) is known to be responsible for some populations to be “deficient” as compared to the population at large, leading to a “poor metabolizer” phenotype, requiring a lower dose. Additionally, exposure to an infectious agent or xenobiotic may cause repression of CYP expression or inhibition of existing CYPs. Alternatively, the patient may be physically weak, injured, or 2014/010714 immunocompromised, all of which might counsel a lower dose. The patient may be taking a number of other drugs which compete with metabolic systems (including CYPs as discussed above) for disposal; this well-know polypharmaceutical effect may call for a lower dose. The dose also depends, as discussed above, on the condition and its severity. The efficacious dose for one disease or clinical endpoint will not necessarily be the same as the dose for another, and a severe, chronic, or otherwise serious case may call for a higher dose. However, a c case may also call for a lower dose administered over a longer or even indefinite period of time. All of these are discussed by way of example to illustrate the variability of ideal dosing; it is within the capacity of the skilled n to select an appropriate dosing range for a disease, population, or individual.

With these factors in mind, it should be clear that it is possible that the daily human dose may be as low as 1 mg/day, and as high as a 1g/day. In certain embodiments, the human dose may range: from 10 mg/day to 500 mg/day, from 20 mg/day to 400 mg/day, or from 25 mg/day to 350 mg/day. In further embodiments, the human dose may range: from120 mg/day to 350 mg/day, from 150 mg/day to 350 mg/day, from 200 mg/day to 350 mg/day, or from 250 mg/day to 350 mg/day. In certain embodiments, the human dose may be any one of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70 75, 80, 85, 90, 95, 100, 110,120, 125, 130, 140, 150, 160, 170, 175, 180, 190, 200, 210, 220, 225, 230, 240, 250, 260, 270, 275, 280, 290, 300, 310, 320, 325, 330, 240 or 350 mg/day.

In certain embodiments, the human dose may be any one of 275, 280, 285, 290, 295, 300, 305, 310, 315, 320, 325, 330, 335, 340, 350, 355, 360, 365, 370, or 375 mg/day. In one embodiment, the dose may be 275 mg/day. In another ment, the dose may be 300 mg/day. In another embodiment, the dose may be 305 mg/day. In another embodiment, the dose may be 310 mg/day. In another embodiment, the dose may be 315 mg/day. In another embodiment, the dose may be 320 mg/day. In another embodiment, the dose may be 325 mg/day. In another ment, the dose may be 330 mg/day. In another embodiment, the dose may be 335 mg/day. In another embodiment, the dose may be 340 mgx’day. In another embodiment, the dose may be 345 mg/day. In another ment, the dose may be 350 mg/day.

In certain ments, the human dose may be any one of 350, 375, 400, 425, 450, 475, 500, 525, 550 or 600 mgfday. In one embodiment, the dose may be 375 mg/day.

In another embodiment, the dose may be 400 mg/day. In r ment, the dose may be 450 mg/day. In another embodiment, the dose may be 500 mg/day.

In certain embodiments, the human dose may be any one of 25, 50, 75, 100, or 125 mg/day. In one embodiment, the dose may be 375 mg/day. In another embodiment, the dose may be 25 . In another embodiment, the dose may be 50 mg/day. In r embodiment, the dose may be 75 mg/day. In another embodiment, the dose may be 100 mg/day. In another embodiment, the dose may be 125 .

IN VIV0 CARRAGEENAN TESTS Carrageenan Paw Test for Edema and Hyperalgesia [05 60] Injection of carrageenan subcutaneously into the hind foot (paw) of a rat or mouse induces robust inflammation and pain. The inflammatory response begins 1-2 hrs post- carrageenan injection and persists for at least five hours following inoculation. In addition, the animal’s inflamed hind paw is sensitive to s (hyperalgesia) or innocuous (allodynia) stimuli, compared to the contralateral hind paw. Compounds can be evaluated in this model for yperalgesia and anti-inflammatory activity. A l increase in threshold or time to d following drug administration suggests analgesic efficacy. A general decrease in paw ng ing drug administration suggests anti-inflammatory efficacy. It is possible that some compounds will affect the inflamed paw and not affect the responses of the contralateral paw.

Embodiments of the carrageenan foot edema test are performed with materials, reagents and procedures essentially as described by Winter, et al., (Proc. Soc. Exp. Biol.

Med, 111, 544 (1962)). Prophylactic and therapeutic embodiments have been developed, and are known in the art. The animals are evaluated for their responsiveness to noxious (paw pinch, plantar test) or innocuous (cold plate, von Frey filaments) stimuli. In the following protocol, mice were used.

Animals, compounds, and dosing. Healthy young male Swiss Webster mice in which weight variation of the mice will not exceed i 20% of the mean were used for the study. Animals were divided into four groups of forty, and each group was dosed by oral gavage with either MGBG (BID, 12 hours apart at 30 mg/kg in 5 mL/kg normal ), dexamethasone as ve l (QD, 1 mg/kg in 5mL/kg 0.5% methylcellulose), or saline vehicle (BID, 5 mL/kg). A fourth group served as naive control (no carrageenan, no treatment). Treatment with MGBG took place on each of three days prior to carrageenan, one hour prior to carrageenan, and 11 hours post-carrageenan. Paw edema is developed by injecting carrageenan (Sigma: k-carrageenan) subcutaneously in the subplantar region of the right paw of the mouse at a volume of 50 uL of 1% carrageenan (w/v) in saline. The contra- lateral paw (left paw) received the same volume (50 uL) of saline and serve as control. Mice will be anesthetized using light dose of ketamine before carrageenan injection.

Paw Edema. Immediately before sub-plantar administration of carrageenan and after 2, 3, 5 and 24 hours post carrageenan, mouse paw volume was measured using the plethysmometer (Ugo Basile). The assessment of edema was expressed as the mean increase in paw volume relative to control.

Assessment ofPaw Withdrawal y. Prior to antar administration of carrageenan and after 0.5, 2, 3, 5, and 24 hours post- carrageenan, the latency of withdrawal response was ined by placing mice on a hot plate analgesia meter with surface temperature maintained at 51°C. A cut-off period of 30s was maintained to avoid any thermal injury to paw. Immediately after testing, all paws were immersed in ice-cold water before returning to the cage. Paw awal latency is calculated as At = right paw withdrawal - left paw withdrawal.

Serum, Plasma, and Histological Collection. Prior to first drug dose on day 0 and at peak disease times (5 and 24 hours post-carrageenan challenge for serum, prior to first drug dose on day 0 and at conclusion of study), serum or plasma was ted from eight mice per group (each) and stored at —700C until cytokine level determination or MGBG drug level determination. For serum collection, whole blood samples are collected in a serum separator tube, processed by fugation and frozen at -70°C. For drug level determination, whole blood samples are collected in a lithium heparin microtainer, processed to plasma by centrifugation and plasma frozen at —70°C. Additionally, paws are collected and preserved in % formalin for histology.

Alternative Protocol. In an alternative embodiment of this assay, MGBG was dosed PO, BID at 3, 10, and 30 mg/kg (with dexamethasone as positive control, saline as negative, and a treatment/carrageenan-naive group, n = 16 each).

Results. MGBG was efficacious in reducing edema and hyperalgesia in the above assay.

Carrageenan Air Pouch Model [05 68] Injection of air subcutaneously will induce the formation of a connective tissue cavity lined with cells that resemble and function like a synovial lining. This method is commonly known as the Air Pouch Model and is a useful animal model of ation that can be generated in a vely short period of time. The air pouch may be created by subcutaneous (SQ) injection of a volume of e air in the dorsal neck region. The model 2014/010714 may be used to test the efficacy and potency of compounds such as polyamine analogues and polyamine biosynthesis inhibitors such as MGBG in reducing various cellular and biochemical indicators of inflammation when administered as an oral (PO) gavage administration.

Animals, compounds, and . Healthy Male Lewis rats (Charles River Laboratories, Wilmington, MA) weighing between 175-200 g were used. MGBG at doses between 1 and 60 mg/kg or vehicle (0.5% methylcellulose, 0.025% 80) were administered by oral gavage (once daily at a volume of 10 mL/kg) for 6 days. A correction factor of 1.49 was used to account for the dihydrochloride salt/monohydrate form of MGB G. en and dexamethasone at doses of 10 and 1 mg/kg, respectively, were dosed 1 day prior to, as well as the g of, carrageenan injection.

Air pouches were formed on the dorsum of the rats during the last 4 days of MGBG or vehicle administration prior to carrageenan injection. Briefly, rats were anesthetized with isoflurane, during which dorsal hair was removed and 20 mL of sterile air was injected subcutaneously into the intrascapular region. Pouches were allowed to develop for the next 4 days, with re-inflation (to maintain pouch ) of pouches 1 day prior to carrageenan injection.

One hour prior to carrageenan injection, s were given their last dose of drug (MGBG, vehicle, naproxen, or dexamethasone). A 1% suspension of carrageenan (2 mL; FMC BioPolyer, elphia, PA) suspended in saline was injected into the pouch cavity. At either 3 hours or 24 hours post-carrageenan injection, rats were euthanized by C02 asphyxiation and 2.5 mL of PBS (Sigma Chemical, St. Louis, MO) was injected directly into the pouch. The pouch was opened with surgical scissors and the pouch fluid was collected using a er e. One aliquot was collected for measurement of total cell count with differential, and the other aliquot for PGEz determination. In the latter case, the aliquot was centrifuged at 1200g for 10 minutes at 40C and the supernatant was collected for analysis of PGE2 via ELISA (Cayman Chemical Company, Ann Arbor, MI). s for the 3- and 24-hour groups were collected as previously bed at either 3 or 24 hours post carrageenan injection.

The protocol may be varied according to s known in the art. Additional tissue may be collected and/or weighed, and additional sectioning, staining, and microscopic examination may be conducted.

Results. MGBG was efficacious in this model, as shown by changes relative to control indicative of reduced inflammation. Fundamentally, MGBG selectively inhibited WO 10154 peak inflammatory production of PGE2, without affecting basal levels of this mediator. By st, both naproxen and dexamethasone inhibited production of both inflammatory and basal levels of this mediator.

IN VIV0 MURINE COLLAGEN-INDUCED ARTHRITIS Collagen-Induced Arthritis Models of Arthritis and Rheumatoid Arthritis The collagen-induced arthritis (CIA) model is ered a suitable model for studying ial drugs active in human arthritis e of the many immunological and pathological similarities to human rheumatoid arthritis (RA), the involvement of localized major histocompatibility, complete class-II-restricted T helper lymphocyte activation, and the similarity of histological lesions. See, e. g., iec EF et al., “Collagen-Induced Arthritis,” Current Protocols in Immunology, Unit 15.5 (1993). See also the model using a onal antibody to CD18 and VLA-4 ins described in Issekutz, A.C. et al., Immunology (1996) 88:569. Features of this CIA model that are similar to that found in RA patients include, without limitation: erosion of cartilage and bone at joint margins (as can be seen in radiographs), proliferative synovitis, symmetrical involvement of small and medium-sized peripheral joints in the appendicular, but not the axial, skeleton. The following ure was followed to assess the efficacy of MGBG in the treatment of arthritic diseases. s and dosing. Inbred male DBA/1 mice (DBA/lOlaHsd, Harlan Laboratories), at least 7 weeks old, may be used in the following collagen-induced arthritis model. Twenty animals per compound or vehicle are assigned to the arthritis and saline groups, 4 to the control group. To induce an arthritic state, mice are etized with isoflurane and given 1501 “L of bovine type II collagen in Freund's complete adjuvant injections (day 0 and day 21). Mice are randomized by body weight into treatment groups on study day 7. Treatment consists of 25 mg/kg MGBG, 0.2 mg/kg dexamethasone as positive l, or saline as vehicle control, all given as oral gavage beginning on study day 0 and continuing daily (PO, BID twice daily/12 hours apart). Twenty mice per group may be used, in which serum is ted from 15 animals, and plasma from five. Four additional animals serve as normal (untreated, non-arthritic) control group. The in-life portion of the study may proceed for 35 days.

Compounds. MGBG solution may be made from the hydrated dihydrochloride salt; other salts could be used, and in any case a salt/hydrate correction factor should be implemented. Solid MGBG can be stored at room temperature, but dose formulations should be made fresh for each administration. thasone is commercially available.

WO 10154 Data. On days 21-35, onset of arthritis typically occurs. During this time clinical scores for paw edema and swelling were given for each of the paws (right front, left front, right rear, left rear). Plasma draws are taken on days 0, l4, and 25 to assess cokinetics, and blood draws taken on days 0 and 28 for disease analysis. Edema is measured on days 18-20, 22-27, and 29-34. Inflammation is assessed by infiltration of atory cells and edema. Post—euthanasia, terminal blood draws are collected, heparinized, and frozen at -700C until analyzed for cytokines such as osteopontin, TNFalpha, IL-l, CRP, MCPl, MIP-lbeta, RANTES, IFNgamma, TGFbeta, IP-10, IL-l7, and MMP9.

Fore and hind paws and knees are collected, and following 1-2 days in fixative and then 4-5 days in decalcifier, processed, embedded, sectioned and stained with toluidine blue for histological is. Bone resorption is quantified by presence of osteoclasts, defects in or loss of medullary trabecular or cortical bone. Cartilage damage is assessed by examining the severity and spread of chondrocyte loss and collagen disruption. Pannus tissue formation and the severity and spread of other evidence of destruction of joint architecture are followed.

Statistical Analysis. al data for paw scores (means for animal) are analyzed by determining the area under the dosing curve (AUC) for days 1-15. For calculation of AUC, the daily mean scores for each mouse are entered into Microsoft Excel and the area between the treatment days after the onset of disease to the termination day is computed.

Means for each group are determined and % inhibition from arthritis controls calculated by comparing values for treated and normal animals. Paw scores and histologic parameters (meaniSE) for each group are ed for differences using a t's t-test with significance set at p 0.05. Percent inhibition of histologic parameters and AUC is calculated as [(mean disease control- mean normal)-(mean treated - mean normal)]/[ [(mean e control- mean normal) ' (mean treated - mean normal)] ‘100.

MGBG was not efficacious in this model as a disease-modifying anti-rheumatic drug (DMARD), but did affect the early phase of paw swelling/inflammation. The protocols above may be varied according to methods known in the art.

IN VIV0 MURINE MOG-EAE MULTIPLE SCLEROSIS MODEL An experimental murine model of multiple sclerosis employing a myelin oligodendrocyte glycoprotein (MOG) peptide to induce mental autoimmune alomyelitis (EAE) was employed to ine the efficacy of MGBG in preventing and ng that disease. e of its many similarities to MS, EAE is commonly used to study pathogenesis of autoimmunity, CNS inflammation, demyelination, cell trafficking, and tolerance induction. EAE is characterized by sis (in some models the paralysis is remitting—relapsing), CNS inflammation and demyelination. EAE is mediated primarily by dendritic cells, myelin-specific T cells (e. g. Th1 and Thl7), and M1 macrophages. B cells may also play a role in some models of EAE. Body weight also tracks disease progression.

EAE is d in C57BL/6 mice by immunization with 55 or MOGst in CFA emulsion followed by administration of pertussis toxin (PTX) in PBS. The emulsion provides antigen which initiates expansion and entiation of MOG-specific autoimmune T cells.

PTX enhances EAE pment by providing additional adjuvant and facilitating entrance of autoimmune T cells into the CNS. [05 82] EAE induction. Chronic EAE develops in 6 mice after immunization with an emulsion of MOG35_55/CFA or MOG1_125/CFA followed by ion of pertussis toxin.

This model is used to test the potential of compounds to prevent or mitigate EAE disease. It can be run with the compound dosed from the time of immunization (prophylactic treatment), or with the aim of reversing the course of disease and facilitating recovery by dosing the compound from the time of EAE onset peutic treatment). The model uses female C57BL/6 mice of age 10 to 14 weeks at the start of the study. Typically, EAE develops 8-18 days after immunization. EAE development is usually followed for 4 weeks (28 days) after zation.

Stress reduces mouse susceptibility to EAE. Aside from any compound effects, the administration of treatment during the disease ion period (~0—10 days after immunization) postpones disease onset and reduces disease severity. This is due to the stress of compound administration and the effects of the vehicle on the mice. The more frequent the administration and the less tolerated the vehicle, the greater the impact on disease development. The stress of treatment and administration of vehicle has much less effect on disease development after al signs of EAE have appeared.

Prophylactic ent. In prophylactic studies, treatment begins before disease onset, at the time of immunization and group assignment. Mice are ed to treatment groups in a balanced manner to achieve groups with similar distributions of body weights.

Prophylactic studies assess if treatment will affect the course of disease both before and after the first clinical signs of EAE. To compensate for the stress of treatment in prophylactic treatment studies and achieve the target disease severity, EAE may be induced with a higher dose of pertussis toxin than used in therapeutic studies. The dose of sis toxin is based on expected stress due to dosing (route, frequency, and formulation of vehicle).

In prophylactic studies, median time to disease onset is a ive measure of compound efficacy. Small s in the immune response can result in postponed disease onset - suppression of T cell activation and proliferation, antigen presentation, differentiation into Th1 and/or Thl7 cells will all result in postponed onset of EAE. Delayed onset of EAE accompanied with lower maximum severity indicates overall efficacy of treatment compared to the negative control group.

Some studies will show postponed EAE onset without other significant compound effects. In these cases the compound may affect an early pathway in immune se development, but eventually redundant processes compensate for the loss of the blocked pathway. Another le explanation is that the drug was not able to maintain the blockade of the pathway for the duration of the study.

In other studies EAE is postponed but mice have higher end EAE scores than the e-treated mice. y this is not caused by the compound making EAE worse but by the peak of disease in the compound-treated mice being postponed and coinciding with the period of recovery for the vehicle-treated mice. [05 88] When a compound is dosed lactically, one important readout of efficacy is ion in maximum disease severity (mean maximum score, MMS). Reduced MMS indicates an overall reduction in EAE severity.

Scoring. Clinically, EAE progression is scored on a scale of 0 to 5, wherein each score represents the following clinical ations: 0 0: no change in function; 0 l: limp tail; 0 2: limp tail and weakness of hind legs; 0 3: one of the following: — limp tail and complete paralysis of hind legs; or - limp tail and complete paralysis of one front and one hind leg; or - severe head tilting, walking along edges of cage, pushing against cage wall, and spinning when picked up by tail; 0 4: limp tail and complete hind leg and partial front leg sis; 0 5: one of the following: - complete front/hind leg paralysis; or - spontaneous rolling in cage; or - death secondary to paralysis.

Course ofEAE development in untreated mice. dual mice will have somewhat differing courses of disease. Most mice show l signs of EAE between 9 and 14 days after immunization. Once EAE starts, the peak of disease almost always occurs 3-4 days later. The maximum score continues for several days and then mice partially recover. In some mice, disease will stay at maximum severity until the end of the study. Less often, a mouse will stay at the peak severity for only one day and then start recovering. The extent of ry largely s on the maximum severity reached by the mouse. Most ted or vehicle-treated mice will not fully recover, but their end score will usually be 0.5 to 1.5 points lower than their maximum score. About 25% of untreated or vehicle-treated mice show worsening EAE between 24 and 28 days after immunization, resembling a relapse.

Spinal cords of these mice at the time of EAE worsening have a large number of inflammatory foci (2 7 foci per section), similar to histological findings at the time of EAE onset and peak, ting that these are true relapses with a new wave of inflammation in the spinal cords. When mice are followed for a longer period of time, disease slowly increases in severity, ling the chronic progressive course of disease ed in human MS patients.

During the course of EAE, changes in body weight reflect e severity. Mice often lose a small amount of weight on the day following immunization. This appears to be due to effects of the administered adjuvant and pertussis toxin. Mice then steadily increase their body weight until disease onset. On the day of EAE onset, mice consistently lose 1-2 g of their body weight (5-10% of body weight). The weight loss continues with the progression of EAE severity, with the loss reaching around 20% of their pre-onset body weight at the peak of disease. The weight loss is most likely due to both paralysis and reduced food intake as well as high production of pro-inflammatory cytokines such as TNF during the acute phase of inflammation. After the peak of disease is reached, mice slowly gain weight, even if their clinical score does not improve. This increase in weight may be due to down tion of inflammation which results in lower levels of pro—inflammatory cytokines in blood.

Untreated or e-treated mice usually have around 90% of their pre-immunization body weight 28 days after immunization.

Histology. Typically, histological analysis is performed either at the end of the study (usually around 28 days after immunization) or at the time when the vehicle group reaches peak of disease (usually 14—18 days after immunization), and s on inflammatory foci, apoptosis, and demyelination, each of which is addressed below.

Inflammation in EAE normally starts in the lumbar region of the spinal cord, spreading to the entire spinal cord by the peak of e. [05 93] Apoptosis. Apoptotic cells are identified in H&E sections, and are usually not found during the first two days of disease pment. They are found at the peak and during the chronic stage of EAE. The average number of apoptotic cells is usually between 2 and 4 per section. The apoptotic cells are neurons and their number correlates with disease . Apoptotic cells appear soon after disease onset, so at EAE onset there will be many inflammatory foci, but few apoptotic cells. Then, the number of apoptotic cells increases until the peak of disease, then remains elevated.

Inflammation. At onset of disease the number of inflammatory foci correlates strongly with disease severity. The number of foci increases somewhat until the peak of e, when 6—15 inflammatory foci on are typically found throughout the spinal cord.

In the chronic stage of EAE (starting several days after the peak of disease), many inflammatory foci resolve, typically resulting in 3—4 inflammatory foci in each spinal cord section by imately 28 days after immunization. [05 95] Because the largest numbers of inflammatory foci are present early in the course of disease, if histological analysis is performed at the end of the study, mice which have late EAE onset often have more atory foci in their spinal cords than might be ed from their clinical score. For example, in a 28 day study a mouse with EAE onset on 27 days after immunization and an end clinical score of 2 will likely have more inflammatory foci than a mouse with EAE onset 9 days after immunization and an end score of 3.5. Similarly, a mouse which relapses shortly before the end of the study (relapse is defined as l or more points of increase in clinical score) will y have more inflammatory foci at the end of the study than a mouse with stable chronic disease, even if the two have the same clinical score at the end of the study. [05 96] Inflammatory foci of approximately 20 cells were counted in each H&E stained section. When inflammatory infiltrates consisted of more than 20 cells, an estimate was made of how many foci of 20 cells were present.

Demyeiination. ination is usually not found during the first two days after disease onset, but is found at the peak of disease (4—5 days after EAE onset) and ues during the chronic phase of EAE. Demyelination scores do not change much between the peak and 28 days after immunization and usually average between 1.2 and 2.5.

Demyelination is scored in both Luxol fast blue stained sections (LFB) and in H&E sections.

In LFB sections, spinal cord white matter stains dark blue and demyelinated areas are a lighter blue color, and are associated with large vacuoles. In H&E stained sections disruption of normal structure with large vacuoles is indicative of ination.

The demyelination score represents an estimate of demyelinated area for each section as s: 0 — no demyelination (less than 5% demyelinated area) 1 — 5 to 20% demyelinated area 2 — 20 to 40% demyelinated area 3 — 40 to 60% demyelinated area 4 — 60 to 80% demyelinated area — 80 to 100% demyelinated area [05 99] For Luxol fast blue stained , the size of the demyelinated area was estimated based on less intense blue staining of myelin. For H&E stained ns, the demyelinated area was estimated by g for interruption of normal structure — pallor and vacuolation consistent with edema and demyelination, and dilated axons.

Statistical analysis. Unless otherwise noted, statistical analysis was performed as follows: disease nce compared using chi-square test; mean day of EAE onset, change in body weight, and number of apoptotic cells compared using 2-tailed Student’s t-test; median day of EAE onset compared using Wilcoxon’s survival test; mean maximum score (MMS), end score, and demyelination scores (LFB and H&E) compared using Wilcoxon’s non-parametric test.

First MOG EAE Protocol.

Materials and Methods. Ten week old female C57BL/6 mice ng 18-23 g (Taconic Farms) were divided into four groups: mock immunized (n = 3) and three MOG- immunized groups receiving MGBG at 30 mpk BID (as the di-HCl monohydrate with a correction factor of 1.49), fingolimod (FTY720) at 3 mpk QD with a second mock vehicle dose, or 0.9% saline vehicle BID (n = 12 each). MOG35.55 peptide was administered by subcutaneous injection at two sites in the back with the emulsion ent ining MOG35_55 for test article or positive control, or PBS for negative control) of Hooke KitTM MOG35_55/CFA Emulsion PTX, catalog number EK—2l 10 (Hooke Laboratories, Lawrence MA). One site of injection was in the area of upper back, approximately 1 cm caudal of the neck line. The second site was in the area of lower back, approximately 2 cm l of the base of the tail. The injection volume was 0.1 mL at each site. Within 2 hours of the injection of on, and then again 24 hours after the injection of emulsion, the pertussis toxin component of the kit (diluted with PBS to achieve 176 ng/dose for the first injection and 165 e for the second injection) was administered intraperitoneally. The volume of each injection was 0.1 mL.

Inoculated, untreated mice develop EAE 8-14 days after immunization with MOG35_55/CFA and stay chronically paralyzed for the 28-day duration of the experiment.

Clinical scores, body weights, and histopathology of spinal cord (e.g., demyelination, inflammatory infiltrates, and/or sis) may be ed and recorded as set forth above.

Results. al scores, given as mean score +I- the standard error of the mean (SEM), are shown in As can be seen in vehicle-treated s developed EAE clinical signs beginning on day 10 as expected, with mean clinical signs increasing until day 16, when scores surpassed 2 and thereafter remained between 2 and 3. Fingolimod was the most efficacious at the high dose tested, yielding mean clinical scores of 0 throughout the experiment, a level almost inguishable from the mock—immunized group. MGBG also prevented onset and progression of EAE, with the first clinical signs ng evident on day 15 and slowly increasing thereafter to a level still below 1 on day 21, and remaining below 1 for the duration of the ment. Results demonstrate that MGBG, like fingolimod, is efficacious in preventing and treating neurological symptoms of MS in an accepted model of the disease.

Additionally, as shown in treatment with either fingolimod or MGBG prevented loss of body weight in the EAE model. s in given as the mean percent change from baseline (study start) +/— SEM, show that all groups continued to gain weight until approximately day 8 when body weights of approximately 107-1 13% were seen.

Thereafter, the vehicle-treated group began to lose weight, with rapid loss to 95% on day 15, and reaching a low of about 93% on day 20 before recovering slightly by the end of the study to about 95%. The sham-immunized and MGBG- and fingolimod- treated groups, generally speaking, each retained weight gained hout the study, hovering between about 108% and about 114%. Fingolimod and vehicle groups demonstrated a slight trend of gradual and continued weight gain over the course of the study. MGB G-treated subjects showed the most substantial gains initially, nearing 115% on day 11, but showed gradual loss to about 108% on day 24, before rising slightly again. These results further demonstrate that MGBG, like fingolimod, is cious in preventing and treating symptoms of MS.

Histopathology. On day 28 (end of the study) all mice were sacrificed for histological analysis and perfused with PBS, and spines were ted in 10% buffered formalin. For each mouse, 3 Luxol fast blue stained sections and 3 H&E sections, from , thoracic, and cervical spinal cord, were prepared and ed by a pathologist blinded to the experimental groups and all clinical readouts. The number of inflammatory foci, apoptotic cells, and demyelinated regions in each of the three H&E sections was determined.

Histopathological results are shown in FIGS 3-5, which confirm that MGBG, like fingolimod, reduces athological signs of MS such as neuroinflammation, demyelination (via both stains), and apoptosis. Additionally, the negative control group (data not shown) demonstrated scores of zero.

Second MOG EAE Protocol.

A second EAE experiment was run substantially as disclosed above, except that fingolimod was dosed at l mpk QD, and the pertussis toxin was administered at 165 e for both injections.

Results. EAE was more severe in this study than in the typical study conducted according to this protocol, and more severe than in the previous study. All mice in the vehicle group developed severe EAE. Disease development in the imod-treated group was not as strongly suppressed as in the previous study or as in the typical study. This is not surprising, given that more severe disease is generally more difficult to suppress. Mice in the MGBG-treated group ped substantially reduced disease ed to that of the vehicle group, displaying able efficacy to that of fingolimod.

Results are given in Figs 6-10. Consistent with the previous study, MGBG was efficacious at postponing disease onset and reducing EAE severity.

Fluorescence-Actz’vated Cell g. In addition to clinical scoring, flow cytometric analysis was performed on CNS-infiltrating cells from 6 mice in each of the vehicle-, fingolimod- and MGBG-treated groups, as well as in all three mice from the mock- immunized (disease-free) group.

On Day 28, the brain and spinal cord tissue (from 6 of 12 mice from each of the MOG-immunized groups; and from all 3 mice from the mock-immunized group) in the second MOG EAE study were pooled and infiltrating cells were isolated by Percoll gradient.

After isolating infiltrating cells from each mouse separately, cells were placed in culture with phorbol myristate acetate (PMA, 50 ng/mL), ionomycin (0.5 ug/mL) and brefeldin (l ug/mL) and ted for 4-5 hours. The cells were then washed and stained for flow cytometric is, as specified below. The following analyses were performed (Becton Dickinson FACScan): WO 10154 0 Anti-CD4-Cy-5/anti-IL—l7A-PE/anti-IFNy-FITC (Th1/Thl7 cells) 0 Anti-CD4-Cy-5/anti-CD1lc-PE/anti-CD45.2-FITC (infiltrating dendritic cells) 0 Anti-CD1lb-Cy-5/anti—IL-lZ-PE/anti-CD45.2-FITC (Ml macrophages) 0 Anti-CD11b-Cy—5/anti—CD206—PE/anti- IL-lO-FITC (M2 macrophages) The number of cells positive for relevant stains and stain ations was ed both as a percentage of the analyzed cells and as the average number of cells per mouse for each group.

Table 8 — Roles of CNS-infiltrating cells and cytokines These cells r EAE. Mice with EAE are known to have more CNS-infiltrating CD4+ cells than disease-free mice, and the number of rating CD4+ cells in the CNS often correlates with disease severity A pro-inflammatory cytokine produced by Th-l7 cells. Th—l7 cells are believed to play a major pathogenic role in EAE development.

IFNy is the main cytokine produced by Th—l cells. While the role CD4+IL- of IFNy in EAE is not clear, it is believed to be highly pathogenic 17A+IFNy+ in EAE.

IFNy is the main cytokine produced by Th-l cells. While the role of IFNy in EAE is not clear, it is believed that Th—l cells, which CD4+IFNy+ produce IFNy play a pathogenic role in EAE, especially during the c phase of disease.

Dendritic cells Play a critical role in presenting antigen to T cells and their (DC) activation. Therefore they play a critical role in controlling CD4—CDl 1c+ immune processes.

CD452 high Part of integrin heterodimer (Mac-l) and is expressed primarily on macrophages (and microglia cells in the CNS) and other cells CD1 lb of the innate immune system (NK cells, granulocytes, some (integrin alpha M) tic cells). Most of these cells are believed to play a pathogenic role in EAE development.

A mannose receptor, which is up-regulated in M2 macrophages (which are ed to suppress Th—l and Th-17 type T cell responses). This is usually ed on hages (CD11b+).

The final enzyme in the urea cycle. It converts L-arginine into L- omithine and urea. It is expressed in cytoplasm and it competes with nitric oxide se for L-arginine. Arginase-l is expressed Arginase-l in altematively-activated macrophages (M2 type macrophages), and plays an important role in ssing T cell functions. This is usually measured on macrophages (CD11b+).

The main marker of dendritic cells. F4/80 is one of the markers of mature macrophages. Therefore CD11c+F4/80- cells are mostly dendritic cells.

An anti-inflammatory cytokine produced primarily by monocytes, macrophages (M2-type), Treg and Th-2 cells.

A cytokine produced by dendritic cells and macrophages. It is essential for Th-l cell entiation and is believed to play an important role in development of autoimmune diseases.

Mice treated with MGBG had a significantly reduced relative and absolute number of CNS infiltrating dendritic cells (DCs, CD45RBhigh, CD11c+) and a significantly reduced number of IL-12—producing cells among CNS-infiltrating CD1 1b—cells. s are given in the tables below, where * indicates p<0.05 and ** indicates p<0. 1. Tables 9 and 11 show results in terms of percentages of total cells, and Tables 10 and 12 shows results in terms of raw numbers of cells (103).

Table 9 % IL- % Total # of 17A+ CD11c+ CNS- % of cells of % CD4+ of CD45.2 infiltrating CD4+ CD4+ IFNy+ high CD4- ent cells (103) cells cells cells cells vehicle 723.33 +/- 34.32 +/- 14.77 +/- 15.75 +/- 12.05 +/- (0.9% saline) 258.43 5.36 2.14 6.98 4.75 % IL- % Total # of 17A+ CD11c+ CNS- cells of of CD45.2 infiltrating CD4+ high CD4- Treatment cells (103) cells cells fingolimod, 620.00 +/- 15.22 +/— 30.89 +/- 11.39 +/— 190.16 2.06 5.13 * 5.36 MGBG, 30 500.00 +/- 15.24 +/- 15.85 +/- 7.21 +/- 142.55 ** 6.02 6.48 1.89 * Negative 286.67 +/— 7.65 +/— 25.82 +/— 11.87 +/— 41.63 * 1.31 * 9.71 5.26 Table 10 Total # of CD45.2 CD4+ ILCNS- high CD4- Treatment CD4+ cells 17A+ rating CD11c+ cells cells (103) cells vehicle 723.33 +/- 245.68 +/- 35.71 +/- 24.70 +/- (0.9 % saline) 258.43 88.17 11 .94 4.88 fingolimod, 620.00 +/- 117.89 +/- 17.67 +/- 18.10 +/- 1mg/kg QD 190.16 76.59 * 12.15 * 4.64 * MGBG, 30 500.00 +/- 152.11 +/- 23.55 +/- 11.90 +/- mg/kg BID 142.55 ** 82.13 ** 17.12 4.14 * Negative EAE 286.67 +/- 30.26 +/- 2.28 +/— 8.87 +/- 1. control 41.63 * 4.25 * 0.21 * 79 * Table 11 Total # of % % IL12+ of CNS- CD206+ IL10+ # of CD45.2 infiltrating % of of of IL12+ high cells CD11b+ CD11b+ CD11b+ cells CDllb- Treatment (10"3) cells cells cells (10"3) cells Total # of IL12+ of CNS- CD206+ IL10+ # of CD452 infiltrating IL12+ high cells CD11b+ cells CD11b- Treatment (10"3) (10"3) cells vehicle 723.33 +/- 1.89 +/- 0.14 +/- (0.9% saline) 258.43 1.15 0.08 fingolimod, 620.00 +/- 1.81 +/- 1.65 +/- 1.86 +/- 0.16 +/— 19016 0.65 1.04 0.12 MGBG, 30 500.00 +/— 1.39 +/— 2.37+/— 0.89 +/— 0.06 +/— 142.55 ** 0.65 0.41 ** 0.06 ** Negative 286.67 +/- 29.94 +/- 2.07 +/- 1.36 +/- 0.20 +/- 0.00 +/- EAE l 41.63 * 2.34 * 1.27 0.82 ** 0.19 * 0.00 * Table 12 Total # of CD45.2 CNS- CD11b+ CD11b+ high CD11b+ IL12+ Treatment infiltrating CD206+ IL10+ CD11b- cells cells cells cells cells IL12+ (10"3) cells vehicle 723.33 +/- 145.80 +/- 2.11 +/- 4.46 +/- 1.89 +/— 0.63 +/- (0 9‘ (70 258.43 37.87 0.43 1.01 1.15 0.40 saline) fingolimod, 620.00 +/- 244.75 +/- 4.07 +/— 4.22 +/- 1.86 +/- 0.41 +/- 1 mg/kg 190.16 95.47 * 1.54 * 2.64 1.04 0.33 MGBG, 30 500.00 +/- 137.88 +/- 1.92 +/- 0.89 +/— 0.21 +/- mg/kg BID 142.55 ** 38.51 1.03 0.41 ** 0.20 * Negative 286.67 +/— 85.54 +/- 1.68 +/— 1.22 +/- 0.20 +i- 0.00 +/— 41.63 * 11 .29 * 0.89 0.87 0.19 * 0.00 * control Third M0G EAE Protocol.

A third EAE experiment was run substantially as disclosed above, except that imod was dosed at 0.1 mpk QD (which corresponds well with the typical human dose or 0.5 mg/day, scaling for body surface area), and an additional group combining MGBG 30 mpk and imod 0.1 mpk was added. Here as well, the pertussis toxin was administered at 165 ng/dose for both injections.

Results. Both fingolimod and MGBG separately showed comparable cy in reducing disease severity and postponing disease onset at the doses tested. There were also significantly fewer inflammatory foci and significantly less demyelination in treated mice compared to the vehicle-treated mice. Importantly, no mice in the imod/MGBG ation group developed EAE, and all other clinical readouts (e.g. body weights) were significantly improved (no signs of EAE) compared to the vehicle group — no disease was detectable in these mice.

Collectively, these results indicate that the combination of fingolimod and MGBG was highly efficacious at preventing development of EAE. In addition, mice which received the combination treatment had icantly improved clinical and ogical readouts compared to the mice ing either fingolimod or MGBG alone. The combination appeared to prevent disease from developing.

Other combinations of agents could be readily tested in a protocol similar to the above. For example, interferon beta—1a, interferon beta-lb, glatiramer e, mitoxantrone, natalizumab, laquinimod, dimethyl fumarate dera), and teriflunomide could each be tested in combination with MGBG. Other SAMDC inhibitors could be tested as well. It is expected that these combinations would be similarly efficacious. Without wishing to be bound by theory, the ation of MGBG, which appears to affect early events in the development of MS such as antigen presentation and the infiltration of dendritic cells into the CNS, should be efficacious in combination with other drugs, for example, those like fingolimod which act at a different (in certain embodiments, later) stage in the development of an e or flare-up of multiple sclerosis, or another demyelinating disease.

Fourth MOG EAE Protocol.

A fourth EAE experiment was run similarly to the above, but was designed to examine the effect of MGBG on cell tions during onset and peak disease. In this protocol, there were 3 experimental groups with 20 mice/group and 1 mock—immunized 2014/010714 group with 8 mice. Fingolimod was dosed at 1 mpk QD, MGBG at 30 mpk BID, and the pertussis toxin was administered at 165 ng/dose for both injections. Flow cytometric analysis was performed on CNS-infiltrating cells from 16 mice from each of the vehicle-, fingolimod- and MGBG-treated groups, as well as from all mice in the mock-immunized group. Half of the analyzed mice from each group were collected at the time of EAE onset in the vehicle group and half were collected at the time of EAE peak in the vehicle group. Mouse termination was over eight te days, because dual mouse in the vehicle group reached EAE onset and EAE peak on different days. Eight (8) of the mice in the vehicle group were sacrificed at each time-point. For each of these mice, a matching mouse from each of Groups 2 and 3 was sacrificed on the same day. For each two (2) of these vehicle group mice, a matching mouse from Group 4 was sacrificed. When selecting matching mice from other groups for termination, the mouse with the t EAE score was chosen. If no mouse in the group had signs of EAE, a mouse was chosen at random.

Results. CNS infiltration by proinflammatory cells was d in MGBG-treated mice, consistent with the clinical g that MGBG reduces EAE development. Multiple proinflammatory cell populations were d in the CNS of MGBG-treated mice.

Interestingly, the proportion and number of infiltrating dendritic cells was reduced in MGB G- treated mice more than in fingolimod-treated mice, and some of the differences were statistically significant. These results are consistent with previous studies, and suggest that MGBG selectively targets dendritic cells. In addition, the reduction in the number of IL-12 producing cells supports the notion that MGBG affects development of Th-l type responses. s are given in the tables below, where * indicates p<0.05 and ** indicates p<0.1. Table 13 shows results in terms of percentages of total cells, and Table 14 shows results in terms of raw numbers of cells (103).

Table 13 Onset Peak % cells unless otherwise indicated % cells unless otherwise indicated fingOh' MGB (') fingOh' (‘) vehicle vehicle MGBG mod G control mod control 31625 655.00 383.75 373.33 1032.50 343.75 346.25 387.50 Total # +/- +/- +/- +/- +/- +/- +/- +/- 2’10?)f 115 107 70 321.11 144.12 * *‘ 60.28 320.35 75.77 * 82.10 * 97.43 * .35 +/- 10.42 8.77 29.42 5.82 +/- 13.40 7.40 +/- 1.21 * +/- +/- +/- 4.50 1.29 * +/- 5.16 2.79 * 3.09 * 2.79 * * 12.22 18.39 17.19 17.25 11.93 CD4+ +/— 5.56 +/— +/— $34538 +/— 5.27 $43133 +/— 4.19 IL-17A+ * 7.30 * 6.00 * ‘ * ‘ * EDEN 2.60 +/— 4J4 134 14.37 4.82 +/— 5.45 +/— 2.20 +/— ' ' ' 1.90 >l< +/_ 2.69 2.54 >l< 3.45 >l< 0.24 IFNy+ 2.75 * 0.76 * .16 CD4+ 957 1333 20.01 17.15 9.37 +/- 15.19 ' ' +/ >l< _ 3.37 +/_ 6.51 +/_ 3.77 1.87 +/_ 4.15 IFNy+ 373 2.40 CD4- CD11c+ CD45.2 high CD11b+ CD11b+ CD206+ CD11b+ Arginase CD206+ Arginase 3.40 +/- 255 255 3.71 +/- 4.22 +/- 4.66 +/- 2.44 +/- CD11c+ ' ' 1.53 >l< 1.15 1.21 2.54 0.69 >l<>l< F4/80- 1.35 * 0.48 * 0413 0.59 +/- 0.62 +/- 0.33 +/- 0.43 * 0.38 * 0.18 * 0 48 IL-12+ ‘ Table 14 Onset Peak # cells (103) # cells (103) . fingoli- MGB (-) . fingoli- (-) vehlcle vehlcle MGBG mod G control mod l 316.25 Total # 655.00 383.75 +/- 373.33 1032.50 343.75 346.25 387.50 of cells +/— +/- 107.70 +/- +/- +/- +/- +/- (103) 321.11 144.12 * * 60.28 320.35 75.77 * 82.10 * 97.43 * 187.68 20.14 32.66 33.38 310.86 20.58 47.51 28.47 +/- +/- 6.78 +/- +/- +/- +/- 8.42 +/- +/- CD4+ 109.88 * 13.83 15.06 119.64 * * 21.25 * 12.01 * 102.99 6.52 6.34 76.35 12.44 CD4+ +/- 2.19 +/- +/- +/- +/- 3.82 +/- +/- 7.83 3.74 +/- IL-17A+ 12.16 0.46 * 4.74 * 5.09 * 39.56 2.54 * * 2.46 * CD4+ 15.70 1.56 0.63 45.90 IL-17A+ +/- 0.50 +/- +/- +/- +/- 1.05 +/- 3.01 +/- 0.61 +/- IFN + 11.50 0.38 * 1.52 * 0.36** 21.79 0.88 * 2.54 * 0.22 * 23.71 3.19 4.08 59.72 CD4+ +/- 3.09 +/- +/- +/- +/- 3.64 +/- 4.57 +/- 4.10 +/- IFN + 15.97 1.35 * 1.73 * 1.78** 24.57 2.06 * 2.52 * 1.68 * 39.32 6.26 7.89 94.45 14.36 17.89 CD11b+ +/- 6.92 +/- +/- +/- +/- +/- +/- 6.61 +/- CD206+ 19.97 2.92 * 2.78 * 5.84 * 31.09 14.30 * 10.67 * 2.77 * CD11b+ Arginase - 14.03 8.93 10.23 39.18 I.- CD11c+ +/— +/_ +/_ +/_ +/— 14.93 16.21 8.95 +/- F4/80- 16.78 12.69 * 5.06 * 2.35 * 19.87 +/_ 7.32 +/- 9.38 1.02 2.33 1.60 .09 +/— 2.93 +/— +/— +/_ 10.78 1.93 +/_ 2.21 +/_ 1.36 +/- IL-12+ 2.88 2.06 1.82 * 1.37 * +/_ 5.36 1.32 1.52 1.00 Table 15 below illustrates the effects seen in a subset of animals which did not develop disease at the time when vehicle-treated animals were at the peak of disease.

MGBG, but not imod, reduced the number of dendritic cells. This suggests that MGBG (and more broadly, a SAMDC inhibitor having an effect on dendritic cell antigen tation and CNS infiltration) may be important component in preventing or reducing the severity of EAE and MS, and/or their progression and associated ms, such as demyelination.

Table 15 Group CD11c+ of CD4— CD45.2+ high cells WO 10154 In Peak of e in Animals Without EAE Disease Fingolimod 5.41 +/- 0.62 MGBG 2.93 +/— 0.37 (—) l 4.64 +/- 0.5 ADDITIONAL IN VIV0 MODELS OF THERAPEUTIC EFFICACY The ing models, ted by way of example, may be used to evaluate compounds disclosed herein for efficacy in the treatment of a number of diseases and indications. It is within the capacity of one skilled in the art to modify these models to suit the needs of the study. Additionally, those skilled in the art will be familiar with onal models of disease which may be employed. It is expected that MGBG, as well as other polyamine analogs and polyamine biosynthesis inhibitors and compounds disclosed herein, will be cious in these models.

Neuropathy and athic Pain Models Bennett Model of Neuropathic Pain: A peripheral mononeuropathy is produced in adult rats by placing loosely constrictive ligatures around the common sciatic nerve. The postoperative behavior of these rats indicates that hyperalgesia, allodynia and, possibly, spontaneous pain (or dysesthesia) were produced. Hyperalgesic responses to noxious radiant heat are typically evident on the second postoperative day and lasted for over 2 . Hyperalgesic responses to chemogenic pain were also present. The presence of allodynia may be inferred from nocifensive responses evoked by standing on an innocuous, chilled metal floor or by innocuous mechanical stimulation (e. g., with von Frey filaments), and by the rats' persistence in holding the hind paw in a d position. The presence of spontaneous pain is suggested by a suppression of appetite and by the frequent occurrence of apparently spontaneous nocifensive responses. The affected hind paw is typically abnormally warm or cool in about one-third of the rats. About one-half of the rats develop grossly overgrown claws on the affected side. In compound efficacy models, test nd is typically delivered prior to stimulation and e serves as control. Experiments with this animal model may advance understanding of the neural mechanisms of neuropathic pain disorders in humans. Bennett GJ, Xie YK, 1988 “A peripheral mononeuropathy in rat that produces disorders of pain ion like those seen in man.,” Pain, Apr;33(1):87-107 (PMID: 2837713).

Chung Model of Neuropathic Pain Since its introduction in 1992, the spinal nerve ligation (SNL) model of neuropathic pain has been widely used for s investigative works on neuropathic pain mechanisms as well as in screening tests for the development of new analgesic drugs. This model was developed by tightly ng one (L5) or two (L5 and L6) segmental spinal nerves in the rat. The operation results in long-lasting behavioral signs of mechanical allodynia, heat hyperalgesia, cold allodynia, and ongoing pain. In the process of widespread usage, many different variations of the SNL model have been produced, either intentionally or ntionally, by different investigators. Although the factors that cause these variations themselves are interesting and important topics to be studied, the pain mechanisms involved in these variations are likely different from the original model. The method for producing the spinal nerve ligation model that will minimally induce potential s that may contribute to these ions is described in detail in Chung JM, Kim HK, and Chung K, “Segmental spinal nerve ligation model of neuropathic pain,” s M01Med.; 2004 99:35-45 (PMID: Chung Model in NHP In a model of painful neuropathy in the primate (Macaca fascicularis), a neuropathic state is induced by tight ligation of the L7 spinal nerve, just distal to the L7 dorsal root on. Sensory testing may be done on the ventral e of the foot, a region that includes the L7 dermatome. Within 1 week following surgery, primates typically develop a marked sensitivity to mechanical stimulation (e. g., with von Frey hairs), indicating the presence of mechanical allodynia. Increased sensitivity to mechanical stimulation is sometimes also observed on the contralateral side. The threshold for withdrawal to a heat stimulus decreases, ting the presence of heat hyperalgesia. Presentation of various cooling stimuli, such as acetone and cold water baths, indicates that cold allodynia also develops. Observed behavioral phenomena are similar to those seen in humans diagnosed with peripheral neuropathic pain. Thus, the model is useful for assessing a number of parameters relevant to human neuropathy and neuropathic pain disorders, and for evaluating the efficacy of drug candidates as treatments for d disorders. See, e.g., Carlton SM et al., ioral manifestations of an experimental model for peripheral athy produced by spinal nerve ligation in the e,” Pain 1994 Feb;56(2):155-66 (PMID: 8008406).

Tactile Allodynia Assessment with Von Frey Filaments The following quantitative allodynia ment technique may be ed to measure tactile allodynia in any of the various animal models of neuropathic pain. The following summary is given by way of example and refers to a rat surgical neuropathy model wherein nocifensive behaviors are evoked by light touch to the paw. Employing von Frey hairs from 0.41 to 15.1 g, the percent response at each stimulus ity may first be characterized. A smooth log-linear relationship is typically observed. Additionally or alternatively, a gm using stimulus oscillation around the se threshold may be employed, which allows more rapid, efficient measurements. Correlation coefficient between the two methods is typically high. In athic rats, good intra- and inter—observer reproducibility is found for the up-down paradigm; some variability may be seen in normal rats, attributable to extensive testing. The fact that thresholds in a sizable group of neuropathic rats show insignificant ility over 20 days, and after 50 days, 61% still met strict neuropathy criteria (using survival analysis), indicates that threshold measurement using the up-down paradigm, in ation with the athic pain model, represents a powerful tool for analyzing the effects of manipulations of the neuropathic pain state. See, e. g., Chaplan SR et al., “Quantitative assessment of tactile allodynia in the rat paw.,” J Neurosci s, 1994 Jul;53(l):55-63 (PMID: 7990513).

Hargreaves Method of Assessing Thermal Nociception Alternatively, a method to measure cutaneous hyperalgesia to thermal stimulation in unrestrained animals has been described. The testing paradigm uses an automated detection of the behavioral end-point; repeated testing does not contribute to the development of the observed hyperalgesia. Carrageenan-induced inflammation results in significantly shorter paw withdrawal latencies as compared to saline-treated paws and these latency changes corresponded to a decreased thermal nociceptive threshold. This ive thermal method detects dose-related hyperalgesia and its blockade by test compounds and allows for the ement of other behavioral parameters in addition to the ptive threshold.

See, e. g., Hargreaves K, et al., “A new and sensitive method for measuring thermal nociception in cutaneous hyperalgesia,” Pain, 1988 Jan;32(1):77-88 (PMID: 3340425).

Inflammatory and Autoimmune Models Contact Dermatitis and Related Disorders Contact hypersensitivity is a simple delayed type hypersensitivity in vivo assay of cell-mediated immune function, which can be used to assess ial therapeutic efficacy in a number of disorders having an inflammatory and/or autoimmune component. Such es include contact dermatitis, atopic dermatitis, psoriasis, allergic dermatitis, and dermal irritation. Compounds may be topically applied, optionally in a topical formulation, or may be red by a non-topical (e. g., oral, IV, etc) route.

Murine Model In one procedure, cutaneous exposure to exogenous haptens gives rise to a delayed type hypersensitivity reaction which is measured and quantitated. Contact sensitivity involves an initial sensitizing phase followed by an elicitation phase. The elicitation phase occurs when the T cytes ter an n to which they have had us contact. Swelling and inflammation occur, making this an excellent model of human allergic t dermatitis. Murine models also lly have the additional benefit of being economical to run. A suitable procedure is described in detail in Gaspari AA and Katz SI, “Contact Hypersensitivity,” Current Protocols in Immunology, Unit 4.2, John Wiley & Sons, Inc. (1994). See also Grabbe S and Schwarz T, “Immunoregulatory mechanisms involved in elicitation of allergic contact hypersensitivity,” Immun. Today 19 (1): 37-44 (1998).

Porcine Model The choice of animal can be important in dematological studies intended to predict human response. For this reason, pigs and in particular minipigs are favored due to the similarities between human and pig skin (particularly follicular density). See, for example, an exemplary model in Bilski AJ and Thomson DS, gic contact itis in the domestic pig. A new model for evaluating the topical anti-inflammatory activity of drugs and their formulations,” Br J Dermatol, 1984 Ju1;111 Suppl 27:143 (PMID: 6743545).

Hairless Guinea Pig Model Allergic and irritant contact ons have also been evaluated in the recently fied hairless guinea pig, Crl:IAF(HA)BR, a mutant from the Hartley strain. The irritant contact dermatitis may be induced by croton oil, 2,4—dinitrochlorobenzene (DNCB), or WO 10154 anthralin. Both hairless and hairy guinea pigs develop similar reactions to these chemicals.

Photoallergic contact ization may be also induced with hlorosalicylanilide (TCSA), or with cyclophosphamide before sensitization with tribromosalicylanilide (TBS).

Cutaneous changes are observed macro- and microscopically according to methods known in the art. Thus, hairless guinea pigs can be used as animal models for assessment of test compounds in the treatment of immunologic and nonimmunologic contact reactions and related disorders. See, e. g., Miyauchi H and Horio T, “A new animal model for contact dermatitis: the hairless guinea pig ,” J Dermatol. 1992 Mar;19(3):l40-5(PMID: 1640019).

Simple dermal irritation may also be studied in hairless guinea-pigs. In an exemplary model, test compounds are delivered in one or more l formulations for 30 min daily exposure for 4 days. Scoring is performed daily; evaporimetry (total epidermal water loss (TEWL)), hydration and colorimetry are measured at ne (day 0) in the middle and at the end of treatment. Test compounds are applied twice daily. See, e.g., en F et al., “The hairless guinea-pig as a model for treatment of cumulative irritation in ,” Skin Res Technol. 2006 Feb;l2(l):60-7 (PMID: 16420540).

Psoriasis Murine Chimera Model Additionally, the compounds disclosed herein can be tested in animal models for psoriasis-like diseases. Research into the cause and pathophysiological mechanisms underlying expression of psoriatric skin lesions has been hampered by lack of an appropriate animal model for this common and enigmatic cutaneous disease. One suitable model is the human skin/scid mouse chimera prepared as described by Nickoloff BJ et al., “Severe combined immunodeficiency mouse and human psoriatic skin chimeras. tion of a new animal model,” Am J Pathol., 1995 6(3): 580-8 (PMID: 7887440) . The methods described therein characterize normal skin, pre-psoriatic skin, and psoriatic plaque skin samples transplanted onto severe combined deficiency mice. Either normal, prepsoriatic, or psoriatic plaque keratome skin samples are transplanted onto severe combined immunodeficiency mice reliably with high rates of graft survival (> 85%) and with reproducible changes consistently observed over prolonged periods of engraftment. After transplantation, by clinical assessment and routine light microscopy, normal skin s essentially normal whereas pre-psoriatic skin became thicker, and psoriatic plaque skin retains its teristic plaque-type elevation and scale. By using a panel of antibodies and histochemical analysis, the overall phenotype of human cell types (including immunocytes) that ted in the transplanted skin was remarkably similar to the phenotype of pretransplanted skin s. Additionally, clearly ized ace zones between human and murine skin within the epidermal and dermal compartments can be identified by routine microscopy and immunostaining, with focal areas of chimerism. The many similarities between pre— and post—transplanted human samples of normal and psoriatic skin that are grafted onto severe combined immunodeficiency mice make this animal model appropriate for use in evaluating test compounds for efficacy in treating psoriasis and related disorders.

Psoriasis Murine scid/scid Model Alternatively, the compounds disclosed herein can be tested in the scid/scid mouse model described by Sch6n MP et al., “Murine sis-like disorder d by naive CD4+ T cells,” Nat Med, 1997 Feb;3(2):183-8 (PMID: 9018237). In this model, titution of scid/scid mice with minor ompatibility mismatched naive CD4+ T lymphocytes results in skin alterations that ngly resemble human psoriasis clinically, histopathologically and in cytokine expression.

Asthma Compounds may additionally be evaluated for efficacy in the treatment of asthma and d pulmonary disorders. In one murine model of asthma, wild-type control /6J, (+/+)] and ICAM-1 (intercellular adhesion molecule-1) knockout [C57BL/6J- ICAM-l, (-/-)] mice are ized to ovalbumin (OVA), and challenged with OVA delivered by aerosol (OVA-OVA) to induce a phenotype consistent with an asthmatic response.

Bronchial responsiveness to methacholine and counts of cell numbers and measurements of eosinophil content and cytokine levels in bronchoalveolar lavage fluid (BALF) may be measured. Additionally, lymphocyte proliferation in response to antigen, eosinophil migration into the s, and the development of airway hyperreactivity (AHR) in allergen— sensitized and -challenged mice may all be measures in vivo or ex vivio according to methods known in the art. See Wolyniec WW et al., “Reduction of antigen—induced airway hyperreactivity and eosinophilia in ICAM—l-deficient mice,” Am J Respir Cell M01 Biol., 1998 Jun;18(6):777—85 (PMID: 9618382).

Inflammatog Bowel Disease, Crohn’s Disease, and Ulcerative Colitis The nds disclosed herein can also be evaluated for activity in animal models of inflammatory bowel disease, Crohn’s disease, and ulcerative colitis. The protocol described by Scheiffele F, Fuss IJ, “Induction of TNBS colitis in mice,” Curr Protoc Immunol, 2002 Aug; Chapter 15:Unit 15.19 (PMID: 18432874), is one of several that have been used to study the immunopathogenesis of these diseases. The model s the use of 2,4,6-trinitrobenzenesulfonic acid , which induces severe colonic inflammation when stered intrarectally in SJL/J mice. The colitis which results from this procedure presents clinical and histopathological findings that resemble those seen in Crohn's disease.

Scheifflele and Fuss discuss the critical parameters needed for successful induction of TNBS— colitis as well methods for monitoring and grading disease levels, and give a support protocol for isolating lamina propria mononuclear cells from mouse colons. See also Morris GP et a1..

“Hapten-induced model of chronic inflammation and ulceration in the rat colon,” Gastroenterology, 1989 Mar;96(3):795-803 (PMID: 2914642), bing the original rat model of chronic colonic inflammation by the uminal instillation of a solution containing a "barrier breaker" (e. g., 0.25 ml of 50% ethanol) and a hapten (e. g., TNBS, 5-30 mg) At a dose of 30 mg, robenzenesulfonic acid/ethanol-induced tion and marked thickening of the bowel wall ted for at least 8 weeks. Histologically, the inflammatory response included l and submucosal infiltration by polymorphonuclear leukocytes, macrophages, lymphocytes, connective tissue mast cells, and fibroblasts. Granulomas (3 wk after induction of inflammation), Langhan's—type giant cells, segmental ulceration and inflammation. The characteristics and relatively long duration of inflammation and ulceration induced in these models afford an opportunity to study the pathophysiology of colonic inflammatory disease in a specifically controlled n, and to evaluate new treatments potentially applicable to inflammatory bowel disease in humans.

EXEMPLARY ORAL PHARMACEUTICAL FORMULATIONS The following are es of compositions which may be used to orally deliver compounds disclosed herein as a capsule.

A solid form of a compound of Formula VI may be passed through one or more sieve s to produce a consistent particle size. Excipients, too, may be passed through a sieve. Appropriate weights of compounds, sufficient to achieve the target dosage per capsule, may be measured and added to a mixing container or apparatus, and the blend is then mixed until uniform. Blend uniformity may be done by, for e, sampling 3 points within the ner (top, middle, and bottom) and testing each sample for potency. A test result of 95— 105% of target, with an RSD of 5%, would be considered ideal; optionally, additional blend time may be allowed to achieve a uniform blend. Upon able blend uniformity results, a measured aliquot of this stock formulation may be ted to manufacture the lower strengths. Magnesium stearate may be passed through a sieve, ted, weighed, added to the blender as a lubricant, and mixed until dispersed. The final blend is weighed and reconciled. Capsules may then be opened and d materials flood fed into the body of the capsules using a spatula. Capsules in trays may be tamped to settle the blend in each capsule to assure uniform target fill weight, then sealed by combining the filled bodies with the caps.

COMPOSITION EXAMPLES In the composition examples below, target dosages may be adjusted to account for the weight of counterions and/or solvates if given as a salt or solvated polymorph thereof. In such a case the weight of the other excipients, typically the filler, is reduced. For example, with the dihydrochloride monohydrate MGBG salt, a correction factor of 1.49 is used (e. g., 360 mg of the salt to give 240.8 mg of the free base).

Example 1A: 300 mg Capsule: Total fill weight of capsule is 500 mg, not including capsule weight. Target compound dosage is 300 mg per e Ingredient Quantity per Capsule, mg MGBG 300.00 Lactose monohydrate 179.00 Silicon dioxide 3.00 Crospovidone 15.00 Magnesium stearate able grade) 3.00 Example 18: J50 mg e: Total fill weight of capsule is 300 mg, not including capsule weight. Target nd dosage is 150 mg per capsule Microcrystalline cellulose (MCC) Magnesium stearate (vegetable grade) MGBG-Fingolimod Combination Examples: Total fill weight of capsule is given below in mg, not ing capsule weight.

WO 10154 Ingredient MGBG Fingolimod . . 0.5 mg 0.25 mg 0.25 mg Mannitol, lactose, and/or microcrystalline cellulose (MCC) Magnesium stearate (vegetable 3 mg 3 mg 3 mg grade) TOTAL FILL 500 mg 300 mg 300 mg 300 mg WEIGHT All references cited herein are orated by reference as if written herein in their entireties. From the foregoing description, one skilled in the art can easily ascertain the essential characteristics of this invention, and without departing from the spirit and scope thereof, can make various changes and modifications of the invention to adapt it to various usages and conditions. The invention disclosed herein provides for embodiments in which each of the embodiments above is combined with one or more of the other ntradictory embodiments, such that the resulting embodiment includes the two or more recited elements and/or limitations.

Claims (20)

WHAT IS CLAIMED IS:

1. Use of MGBG in the manufacture of a medicament for treatment of progressive multiple sclerosis in a patient.

2. The use as recited in claim 1,wherein the progressive multiple sclerosis is primary progressive.

3. The use as recited in claim 1, wherein the progressive multiple sclerosis is ary progressive.

4. The use as recited in claim 1, wherein the progressive multiple sclerosis is progressive relapsing.

5. The use as recited in claim 1, wherein the MGBG is formulated for oral administration.

6. The use as d in claim 5, wherein the MGBG is formulated for dosage at 20 mg/day to 400 mg/day.

7. The use as d in claim 6, wherein the MGBG is formulated for co-administration with an agent chosen from interferon beta-1a, interferon beta-1b, amer acetate, mitoxantrone, natalizumab, fingolimod, laquinimod, dimethyl fumarate, and unomide.

8. The use as recited in claim 7, wherein the agent is fingolimod.

9. The use as recited in claim 8, wherein the fingolimod is formulated for dosage at 0.5 mg per day.

10. The use as recited in claim 8, wherein the fingolimod is formulated for dosage at less than 0.5 mg per day.

11. The use as recited in claim 8, wherein the fingolimod is formulated for dosage at 0.25 mg per day.

12. The use as recited in claim 1 or 8, n the medicament is to prevent relapse or progression of MS.

13. The use as recited in claim 1 or 8, wherein the ment has a reduced incidence of at least one side effect chosen from nia, toxicity, hepatotoxicity, cardiotoxicity, teratogenicity, decreased pulmonary function, macular edema, peripheral athy, severe skin reactions, increased risk of infections , impairment of innate immunity, impairment of adaptive immunity, and flushing, as compared to interferon beta-1a, interferon beta-1b, glatiramer acetate, teriflunomide, fingolimod, Tecfidera (dimethyl fumarate), mitoxantrone, or natalizumab.

14. The use as recited in claim 13, wherein the medicament has a reduced incidence of at least one side effect chosen from cytopenia, nephrotoxicity, toxicity, cardiotoxicity, and teratogenicity.

15. The use as recited in claim 14, wherein the cytopenia is chosen from lymphopenia and neutropenia.

16. The use as recited in claim 13, wherein the medicament has a reduced incidence of at least two side effects chosen from cytopenia, nephrotoxicity, hepatotoxicity, cardiotoxicity, and teratogenicity.

17. The use as recited in claim 16, wherein the cytopenia is chosen from lymphopenia and neutropenia.

18. The use as recited in claim 16, wherein the medicament has a reduced incidence of cytopenia, nephrotoxicity, and hepatotoxicity.

19. The use as recited in claim 18, wherein the cytopenia is chosen from penia and neutropenia.

20. The use as d in claim 19, n the medicament has a reduced incidence of cardiotoxicity, and teratogenicity.