NZ516885A - Introducing into a cell a nucleic acid fragment targetted to an amplifiable region of a chromosome whereby the fragment integrates into the chromosome and further identifying such cells - Google Patents
Introducing into a cell a nucleic acid fragment targetted to an amplifiable region of a chromosome whereby the fragment integrates into the chromosome and further identifying such cellsInfo
- Publication number
- NZ516885A NZ516885A NZ516885A NZ51688597A NZ516885A NZ 516885 A NZ516885 A NZ 516885A NZ 516885 A NZ516885 A NZ 516885A NZ 51688597 A NZ51688597 A NZ 51688597A NZ 516885 A NZ516885 A NZ 516885A
- Authority
- NZ
- New Zealand
- Prior art keywords
- chromosome
- nucleic acid
- cell
- fragment
- introducing
- Prior art date
Links
- 210000004027 cell Anatomy 0.000 title abstract 7
- 150000007523 nucleic acids Chemical group 0.000 title abstract 5
- 210000000349 chromosome Anatomy 0.000 title abstract 4
- 239000012634 fragment Substances 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 abstract 4
- 108020004707 nucleic acids Proteins 0.000 abstract 4
- 238000000034 method Methods 0.000 abstract 3
- 230000003321 amplification Effects 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 210000004507 artificial chromosome Anatomy 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
A method for amplifying nucleic acid comprising introducing a nucleic acid molecule into a cell, wherein the nucleic acid molecule includes a sequence of nucleotides that target it to an amplification region of the chromosome in the cell. The cells are grown and a cell that includes a chromosome with a portion that has undergone amplification is identified. The nucleic acid includes sequences from an rDNA unit. The method is related to methods for preparing cell lines that contain artificial chromosomes.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US62982296A | 1996-04-10 | 1996-04-10 | |
| US08/682,080 US6077697A (en) | 1996-04-10 | 1996-07-15 | Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes |
| US08/695,191 US6025155A (en) | 1996-04-10 | 1996-08-07 | Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes |
| NZ503055A NZ503055A (en) | 1996-04-10 | 1997-04-10 | Artificial chromosomes generated by culturing cells with dicentric chromosomes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NZ516885A true NZ516885A (en) | 2002-08-28 |
Family
ID=27484388
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NZ516885A NZ516885A (en) | 1996-04-10 | 1997-04-10 | Introducing into a cell a nucleic acid fragment targetted to an amplifiable region of a chromosome whereby the fragment integrates into the chromosome and further identifying such cells |
Country Status (1)
| Country | Link |
|---|---|
| NZ (1) | NZ516885A (en) |
-
1997
- 1997-04-10 NZ NZ516885A patent/NZ516885A/en not_active IP Right Cessation
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PSEA | Patent sealed | ||
| RENW | Renewal (renewal fees accepted) | ||
| RENW | Renewal (renewal fees accepted) | ||
| RENW | Renewal (renewal fees accepted) | ||
| RENW | Renewal (renewal fees accepted) | ||
| EXPY | Patent expired |