NO903788L - MONOCLONAL ANTIBODY AGAINST PROTEIN C. - Google Patents
MONOCLONAL ANTIBODY AGAINST PROTEIN C.Info
- Publication number
- NO903788L NO903788L NO90903788A NO903788A NO903788L NO 903788 L NO903788 L NO 903788L NO 90903788 A NO90903788 A NO 90903788A NO 903788 A NO903788 A NO 903788A NO 903788 L NO903788 L NO 903788L
- Authority
- NO
- Norway
- Prior art keywords
- protein
- antibody
- activation
- isolation
- peptide
- Prior art date
Links
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- Peptides Or Proteins (AREA)
Abstract
Ca2+-avhengig monoklonalt antistoff som spesifikt binder til en spesifikk tolv-peptidsekvens (EDQVDPRLIDGK) i aktiveringsregionen i protein C. Antistoffet binder ikke til aktivert protein C og benyttes for å hemme aktivering av protein C ved trombin-trombomodulin. Antistoffet kan isoleres fra cellekultur eller ascitesvæske i store mengder ved affinitetskromatografi under milde betingelser ved bruk av peptidet bundet til et immobilisert substrat. Antistoffet har flere spesifikke anvendelser ved isolering og karakterisering av protein C og som en modell for konstruk- sjon av Ca2+-avhengig antistoffer for isoleringen av andre proteiner, som et diagnostikum, og som et terapeutikum for å hindre aktivering av protein C. Protein C kan produseres naturlig eller produseres ved ekspresjon av det rekombinante genet. Fordelene med antistoffet i rensingen av protein C inkluderer spesifisiteten for protein C og ikke for aktivert protein C, og den unike Ca2+-peptidbindingsspesifisiteten som tillater bindingssetet å være beskyttet når det er immobilisert på det kromatografiske underlag. In vivo har antistoffet blitt demonstrert å hemme tumorvekst. Antistoffet kan også benyttes for å fremme koagulasjon hos pasienter som har høye nivåer av faktor VTII-inhibitorer.Ca 2+ -dependent monoclonal antibody that specifically binds to a specific twelve-peptide sequence (EDQVDPRLIDGK) in the activation region of protein C. The antibody does not bind to activated protein C and is used to inhibit activation of protein C by thrombin thrombomodulin. The antibody can be isolated from cell culture or ascites fluid in large quantities by affinity chromatography under mild conditions using the peptide bound to an immobilized substrate. The antibody has several specific uses for the isolation and characterization of protein C and as a model for the construction of Ca 2+ -dependent antibodies for the isolation of other proteins, as a diagnostic agent, and as a therapeutic agent to prevent activation of protein C. Protein C produced naturally or produced by expression of the recombinant gene. The benefits of the antibody in the purification of protein C include the specificity of protein C and not of activated protein C, and the unique Ca 2+ peptide binding specificity that allows the binding site to be protected when immobilized on the chromatographic substrate. In vivo, the antibody has been demonstrated to inhibit tumor growth. The antibody can also be used to promote coagulation in patients who have high levels of factor VTII inhibitors.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US29244788A | 1988-12-30 | 1988-12-30 | |
| PCT/US1989/005851 WO1990007524A1 (en) | 1988-12-30 | 1989-12-28 | Monoclonal antibody against protein c |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| NO903788D0 NO903788D0 (en) | 1990-08-29 |
| NO903788L true NO903788L (en) | 1990-10-29 |
Family
ID=26780202
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO90903788A NO903788L (en) | 1988-12-30 | 1990-08-29 | MONOCLONAL ANTIBODY AGAINST PROTEIN C. |
Country Status (1)
| Country | Link |
|---|---|
| NO (1) | NO903788L (en) |
-
1990
- 1990-08-29 NO NO90903788A patent/NO903788L/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| NO903788D0 (en) | 1990-08-29 |
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