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NO904133L - PROCEDURE FOR SEQUENCE DETERMINATION OF DNA. - Google Patents

PROCEDURE FOR SEQUENCE DETERMINATION OF DNA.

Info

Publication number
NO904133L
NO904133L NO90904133A NO904133A NO904133L NO 904133 L NO904133 L NO 904133L NO 90904133 A NO90904133 A NO 90904133A NO 904133 A NO904133 A NO 904133A NO 904133 L NO904133 L NO 904133L
Authority
NO
Norway
Prior art keywords
dna
chain
procedure
sequence determination
immobilized
Prior art date
Application number
NO90904133A
Other languages
Norwegian (no)
Other versions
NO904133D0 (en
Inventor
Mathias Uhlen
Original Assignee
Cemu Bioteknik Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from SE8801070A external-priority patent/SE8801070D0/en
Application filed by Cemu Bioteknik Ab filed Critical Cemu Bioteknik Ab
Publication of NO904133D0 publication Critical patent/NO904133D0/en
Publication of NO904133L publication Critical patent/NO904133L/en

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Fremgangsmåte for sekvensbestemmelse av mål-DNA, hvor DNA tilveiebringes i dobbeltkjedet form, immobilisert på en fast bærer via en ende av en av de to kjeder av denne, og så underkastes kjedeseparasjon hvorved den ikke-tilheftede kjede fjernes for sekvensbestemmelse av den immobiliserte kjede. Mål-DNA, f.eks. genom-DNA, kan i begynnelsen forokes ved PCR- metoden under anvendelse av minst en oligonukleotidprimer som ! enten er festet til en fast bærer eller bærer anordning for tilhefting til en fast bærer.Method for sequencing target DNA, where DNA is provided in double-chain form, immobilized on a solid support through one end of one of its two chains, and then subjected to chain separation, thereby removing the non-adhered chain for sequencing the immobilized chain. Target DNA, e.g. genomic DNA can be initially digested by the PCR method using at least one oligonucleotide primer such as is either attached to a fixed support or carrier device for attachment to a fixed support.

NO90904133A 1988-03-23 1990-09-21 PROCEDURE FOR SEQUENCE DETERMINATION OF DNA. NO904133L (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE8801070A SE8801070D0 (en) 1988-03-23 1988-03-23 METHOD FOR IMMOBILIZING A DNA SEQUENCE ON A SOLID SUPPORT
PCT/GB1989/000304 WO1989009282A1 (en) 1988-03-23 1989-03-22 Method of sequencing dna

Publications (2)

Publication Number Publication Date
NO904133D0 NO904133D0 (en) 1990-09-21
NO904133L true NO904133L (en) 1990-11-21

Family

ID=26294810

Family Applications (1)

Application Number Title Priority Date Filing Date
NO90904133A NO904133L (en) 1988-03-23 1990-09-21 PROCEDURE FOR SEQUENCE DETERMINATION OF DNA.

Country Status (2)

Country Link
DE (1) DE406296T1 (en)
NO (1) NO904133L (en)

Also Published As

Publication number Publication date
NO904133D0 (en) 1990-09-21
DE406296T1 (en) 1992-06-11

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