NO174915B - Cosmetic preparation - Google Patents
Cosmetic preparation Download PDFInfo
- Publication number
- NO174915B NO174915B NO893815A NO893815A NO174915B NO 174915 B NO174915 B NO 174915B NO 893815 A NO893815 A NO 893815A NO 893815 A NO893815 A NO 893815A NO 174915 B NO174915 B NO 174915B
- Authority
- NO
- Norway
- Prior art keywords
- extract
- reaction
- skin
- cosmetic preparation
- aging
- Prior art date
Links
- 238000002360 preparation method Methods 0.000 title claims description 13
- 239000002537 cosmetic Substances 0.000 title claims description 12
- 239000000284 extract Substances 0.000 claims description 25
- 244000298479 Cichorium intybus Species 0.000 claims description 10
- 235000007542 Cichorium intybus Nutrition 0.000 claims description 10
- 230000032683 aging Effects 0.000 claims description 6
- 150000008442 polyphenolic compounds Chemical class 0.000 claims description 4
- 235000013824 polyphenols Nutrition 0.000 claims description 4
- 239000004480 active ingredient Substances 0.000 claims description 3
- 239000002798 polar solvent Substances 0.000 claims description 3
- 241000723343 Cichorium Species 0.000 claims description 2
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 15
- 150000003254 radicals Chemical class 0.000 description 14
- 238000006243 chemical reaction Methods 0.000 description 12
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 9
- 210000003491 skin Anatomy 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 5
- -1 peroxide anion Chemical class 0.000 description 5
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 229960001484 edetic acid Drugs 0.000 description 4
- 229910052742 iron Inorganic materials 0.000 description 4
- 150000003904 phospholipids Chemical class 0.000 description 4
- ASJSAQIRZKANQN-CRCLSJGQSA-N 2-deoxy-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)CC=O ASJSAQIRZKANQN-CRCLSJGQSA-N 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 239000006071 cream Substances 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000005502 peroxidation Methods 0.000 description 3
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 description 2
- RVBUGGBMJDPOST-UHFFFAOYSA-N 2-thiobarbituric acid Chemical compound O=C1CC(=O)NC(=S)N1 RVBUGGBMJDPOST-UHFFFAOYSA-N 0.000 description 2
- 102000016938 Catalase Human genes 0.000 description 2
- 108010053835 Catalase Proteins 0.000 description 2
- 102000008186 Collagen Human genes 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- 102000016942 Elastin Human genes 0.000 description 2
- 108010014258 Elastin Proteins 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 238000006993 Weiss annulation reaction Methods 0.000 description 2
- 108010093894 Xanthine oxidase Proteins 0.000 description 2
- 230000003796 beauty Effects 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- SWGKAHCIOQPKFW-GHMZBOCLSA-N caffeoyltartaric acid Natural products OC(=O)[C@H](O)[C@H](C(O)=O)OC(=O)C=CC1=CC=C(O)C(O)=C1 SWGKAHCIOQPKFW-GHMZBOCLSA-N 0.000 description 2
- 229920001436 collagen Polymers 0.000 description 2
- 229920002549 elastin Polymers 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- BXWNKGSJHAJOGX-UHFFFAOYSA-N hexadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCO BXWNKGSJHAJOGX-UHFFFAOYSA-N 0.000 description 2
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 239000002502 liposome Substances 0.000 description 2
- 239000006210 lotion Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 150000002978 peroxides Chemical class 0.000 description 2
- 238000006552 photochemical reaction Methods 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000009759 skin aging Effects 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- ASJSAQIRZKANQN-UHFFFAOYSA-N 2-deoxypentose Chemical compound OCC(O)C(O)CC=O ASJSAQIRZKANQN-UHFFFAOYSA-N 0.000 description 1
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 1
- 239000004166 Lanolin Substances 0.000 description 1
- 239000002262 Schiff base Substances 0.000 description 1
- 150000004753 Schiff bases Chemical class 0.000 description 1
- 102100033220 Xanthine oxidase Human genes 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 239000010775 animal oil Substances 0.000 description 1
- 230000002225 anti-radical effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 238000003287 bathing Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000032677 cell aging Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 229960000541 cetyl alcohol Drugs 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000000731 choleretic agent Substances 0.000 description 1
- 230000001989 choleretic effect Effects 0.000 description 1
- 230000000536 complexating effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000002934 diuretic Substances 0.000 description 1
- 230000001882 diuretic effect Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 229940124600 folk medicine Drugs 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 229960005150 glycerol Drugs 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 239000003906 humectant Substances 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- TUJKJAMUKRIRHC-UHFFFAOYSA-N hydroxyl Chemical compound [OH] TUJKJAMUKRIRHC-UHFFFAOYSA-N 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 229910052743 krypton Inorganic materials 0.000 description 1
- DNNSSWSSYDEUBZ-UHFFFAOYSA-N krypton atom Chemical compound [Kr] DNNSSWSSYDEUBZ-UHFFFAOYSA-N 0.000 description 1
- 229940039717 lanolin Drugs 0.000 description 1
- 235000019388 lanolin Nutrition 0.000 description 1
- 230000002475 laxative effect Effects 0.000 description 1
- 238000002803 maceration Methods 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 230000006540 mitochondrial respiration Effects 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920005862 polyol Polymers 0.000 description 1
- 150000003077 polyols Chemical class 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 150000003141 primary amines Chemical class 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 229960004063 propylene glycol Drugs 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
Landscapes
- Cosmetics (AREA)
Description
Foreliggende oppfinnelse vedrører et kosmetisk preparat for The present invention relates to a cosmetic preparation for
forhindring av hudens aldring. Preparatet er kjennetegnet ved at det som aktiv bestanddel inneholder en ekstrakt, tilstede i en konsentrasjon uttrykt som polyfenoler på fra 0,001 til 0,01 vekt%, av luftdelene av Cichorium intybus L, idet ekstrakten prevention of skin aging. The preparation is characterized by the fact that it contains as active ingredient an extract, present in a concentration expressed as polyphenols of from 0.001 to 0.01% by weight, of the aerial parts of Cichorium intybus L, as the extract
er utvunnet ved hjelp av et polart løsningsmiddel fra luftdelen av Cichorium intibys L. is extracted using a polar solvent from the aerial part of Cichorium intibys L.
Ekstrakten av Cichorium intybus L (forbindelser) inneholder forskjellige polyfenoliske derivater som f.eks. kaffeylvinsyre. The extract of Cichorium intybus L (compounds) contains various polyphenolic derivatives such as caffeyl tartaric acid.
Ekstrakten av Cichorium intybus L kan fremstilles ved ekstrak-sjon av de tørkede luftdeler ved hjelp av polare løsnings-midler som kokende vann, metanol, etanol og propylenglykol. The extract of Cichorium intybus L can be prepared by extracting the dried aerial parts using polar solvents such as boiling water, methanol, ethanol and propylene glycol.
Bladene av denne planten er kjent for deres choleretiske, diuretiske og svakt avførende egenskaper som er grunnen til at infusjoner av ekstraktene har vært anvendt som et blodrensende middel innen folkemedisinen. The leaves of this plant are known for their choleretic, diuretic and slightly laxative properties, which is why infusions of the extracts have been used as a blood purifier in folk medicine.
Grunnlaget for den foreliggende oppfinnelse er erkjennelsen om at en ekstrakt av luftdelene av Cichorium intybus L har en antiradikal aktivitet, særlig som et resultat av kompleksdan-nende jern, og at kosmetiske preparater inneholdende en slik ekstrakt kan forhindre de skadelige virkninger av de frie radikaler som delvis er ansvarlig for hudens aldring. The basis of the present invention is the recognition that an extract of the aerial parts of Cichorium intybus L has an antiradical activity, particularly as a result of complexing iron, and that cosmetic preparations containing such an extract can prevent the harmful effects of the free radicals which is partly responsible for skin aging.
De frie radikalene frembringes under reaksjonene med celle-metabolisme, særlig som et resultat av ett-elektronreduksjonen av oksygen under reaksjonene med mitokondrien respirasjon. The free radicals are produced during the reactions with cell metabolism, particularly as a result of the one-electron reduction of oxygen during the reactions with mitochondrial respiration.
De frembringes også i huden ved fotokjemiske reaksjoner under eksponering for sollys. Det foreligger et kraftig beskyt-telsessystem mot slike radikaler i en intakt celle. Peroksydanionet (02.-) omdannes ved hjelp av peroksyddismutaser til hydrogenperoksyd (H202) som i sin tur metaboliseres til vann (H20) ved katalase. Peroksyddismutase + katalase paret er et avgiftingssystem hvis effektivitet nedsettes under aldring. Under disse betingelser reagerer 02.- og H202 for å utvikle en kaskade av reaksjoner som katalyseres av jern og som er kjent som HABER-WEISS-reaksjonen, og som særlig leder til dannelse av hydroksyl (0H- )-radikalet. Dette radikal fremviser en meget høy reaktivitet og påvirker gjensidig biologiske struk-turer, særlig membranfosfolipidene, proteinene og nuklein-syrene, som det skader. They are also produced in the skin by photochemical reactions during exposure to sunlight. There is a powerful protection system against such radicals in an intact cell. The peroxide anion (02.-) is converted by peroxide dismutases into hydrogen peroxide (H202), which in turn is metabolized to water (H20) by catalase. The peroxide dismutase + catalase pair is a detoxification system whose efficiency decreases during aging. Under these conditions, O 2 and H 2 O 2 react to develop a cascade of reactions catalyzed by iron and known as the HABER-WEISS reaction, which in particular leads to the formation of the hydroxyl (OH- ) radical. This radical exhibits a very high reactivity and mutually affects biological structures, particularly the membrane phospholipids, proteins and nucleic acids, which it damages.
Det er kjent at hverken peroksydanionet eller hydrogenperoksyd kan indusere peroksydering av lipider eller et sukker som f.eks. deoksyribose direkte. It is known that neither the peroxide anion nor hydrogen peroxide can induce peroxidation of lipids or a sugar such as e.g. deoxyribose directly.
Denne reaksjon krever faktisk dannelse av mer reaktive radikaler som hydroksylradikalet OH*, som katalyseres ved hjelp av jern. This reaction actually requires the formation of more reactive radicals such as the hydroxyl radical OH*, which is catalyzed by iron.
Når slike radikaler angriper fettsyrene i membranfosfolipidene sprer det seg en desorganisering gjennom cellemembranene som inhiberer deres funksjoner og særlig med hensyn til epidermis, forstyrrer deres rolle som barriere som sikrer adekvat kutan hydratisering. På samme måte, når disse radikaler gjensidig påvirker proteinene i huden som f.eks. elastin og kollagen, medfører de brudd og kryssbindingsegenskaper ved kutan aldring. When such radicals attack the fatty acids in the membrane phospholipids, a disorganization spreads through the cell membranes that inhibits their functions and, particularly with regard to the epidermis, disrupts their role as a barrier that ensures adequate cutaneous hydration. In the same way, when these radicals mutually affect the proteins in the skin, such as elastin and collagen, they cause breakage and cross-linking properties during cutaneous ageing.
Som en følge av nærværet av ekstrakten av luftdelene av Cichorium intybus L som inhiberer disse frie radikaler, særlig ved å chelatere jern, er det kosmetiske preparat i samsvar med oppfinnelsen i stand til å forhindre aldring av huden. As a result of the presence of the extract of the aerial parts of Cichorium intybus L which inhibits these free radicals, in particular by chelating iron, the cosmetic preparation according to the invention is able to prevent aging of the skin.
Det kosmetiske preparat ifølge oppfinnelsen muliggjør forhindring av aldring av huden når minst en ektrakt av luftdelene av Cichorium intybus L påføres huden i en passende kosmetisk base. The cosmetic preparation according to the invention enables the prevention of aging of the skin when at least one extract of the aerial parts of Cichorium intybus L is applied to the skin in a suitable cosmetic base.
Ekstrakten kan fremstilles på følgende måte. The extract can be prepared in the following way.
Eksempel på fremstilling av ekstrakten Example of preparation of the extract
Luftdelene av Cichorium intybus L tørkes og findeles (uten faktisk å males til et pulver). De ekstraheres med fem ganger sin egen vekt av kokende vann (macerasjon i 30 minutter). Ekstrakten filtreres, og deretter ekstraheres resten med propylenglykol (fem ganger den initiale vekt av plantedelen). Propylenglykolekstrakten filtreres, resten tørkes ved hjelp av en presse og ekstraktene samles. The aerial parts of Cichorium intybus L are dried and crushed (without actually being ground into a powder). They are extracted with five times their own weight of boiling water (maceration for 30 minutes). The extract is filtered, and then the residue is extracted with propylene glycol (five times the initial weight of the plant part). The propylene glycol extract is filtered, the residue is dried using a press and the extracts are collected.
Vann/propylenglykolekstrakten (50:50) inneholder 1 ± 0,2 mg/ml polyfenoler som inkluderer kaffeylvinsyre (målt ved hjelp av ARNOW-reagenset). The water/propylene glycol extract (50:50) contains 1 ± 0.2 mg/ml polyphenols including caffeyl tartaric acid (measured using the ARNOW reagent).
Den følgende forsøksprotokoll muliggjør påvisning av aktivi-teten av ekstrakten/den aktive bestanddel anvendt ved oppfinnelsen. The following test protocol enables detection of the activity of the extract/active ingredient used in the invention.
Inhibering av Fenton-reaksjonen Inhibition of the Fenton reaction
Fenton-reaksjonen er det tredje trinn i HABER-WEISS-reaksjonen. The Fenton reaction is the third step in the HABER-WEISS reaction.
Denne reaksjon gjomføres in vitro ved å omsette H202 (10"<4>M) This reaction is carried out in vitro by reacting H2O2 (10"<4>M)
+ Fe<2+> (10"<5>M) i nærvær av hypoxantin og xantinoksydase, som utvikler peroksydanionet og deoksyribose (5 x 10"<3>M), som er substratet ved peroksydasjonsreaksjonen. + Fe<2+> (10"<5>M) in the presence of hypoxanthine and xanthine oxidase, which develop the peroxide anion and deoxyribose (5 x 10"<3>M), which is the substrate of the peroxidation reaction.
Når reaksjonen gjennomføres i nærvær av EDTA (etylendiamin-tetraeddiksyre) blir radikalet utviklet som 0H- i samsvar med den ovenfor beskrevne reaksjon. I motsetning til dette utvikles, i fravær av EDTA, en annen fremdeles ukjent type radikal (WINTERBOURN, CC. Free Radical Biology & Medicine, 3, 33 - 39, 1987). When the reaction is carried out in the presence of EDTA (ethylenediamine-tetraacetic acid), the radical is developed as OH- in accordance with the reaction described above. In contrast, in the absence of EDTA, another still unknown type of radical is developed (WINTERBOURN, CC. Free Radical Biology & Medicine, 3, 33 - 39, 1987).
I fravær eller nærvær av EDTA omdanner reaksjonen deoksyribose til oksydasjonsprodukter som kan måles ved hjelp av tiobar-bitursyre i samsvar med metoden beskrevet av GUTTERIDGE, J.M.C. (Biochem. J., 224, 761 - 767, 1984). In the absence or presence of EDTA, the reaction converts deoxyribose to oxidation products which can be measured by thiobarbituric acid according to the method described by GUTTERIDGE, J.M.C. (Biochem. J., 224, 761-767, 1984).
De aktive bestanddeler av ekstrakten av Cichorium intybus L inhiberer oksydasjonen av deoksyribose ved tilsetning til reaksjonsblandingen under de to sett av betingelser: The active constituents of the extract of Cichorium intybus L inhibit the oxidation of deoxyribose when added to the reaction mixture under the two sets of conditions:
Inhibering av dannelse av kutant malonaldehyd Inhibition of cutaneous malonaldehyde formation
Et av tegnene på cellulær aldring som impliserer frie radikaler i aldringsprosessen er akkumulering av lipofuskiner i vevene. Disse pigmenter er Schiffske baser som resulterer fra reaksjonen av primære aminer med det malonaldehyd (MDA) som produseres ved radikal destruksjon av lipider og visse sukkerarter. One of the signs of cellular aging that implicates free radicals in the aging process is the accumulation of lipofuscins in the tissues. These pigments are Schiff bases resulting from the reaction of primary amines with the malonaldehyde (MDA) produced by the radical destruction of lipids and certain sugars.
Den beskyttende virkning av ekstrakten mot denne prosess kan påvises ved en test hvor ekstrakten, når den er fortynnet i et kosmetisk hjelpestoff og påføres huden på et forsøksdyr, inhiberer dannelse av MDA som normalt induseres ved bestråling i det kutane vev. Et typisk forsøk er vist i det følgende: Hårløse mus ble sensitivert ved bading i fem minutter i en oppløsning av Bengalrose (10"<5>M) og deretter tørket i mørke. Den fortynnede ekstrakt eller hjelpestoffet ble påført over-flaten av den dorsale hud på dyrene ti minutter før de ble bestrålt. Bestålingen ble gjennomført i 3 0 munutter ved hjelp av en 75 watt KRYPTON glødelampe anbragt i en avstand på 25 cm fra dyrene. Kontrollmus ble holdt i mørke. Dyrene ble avlivet og prøver av deres epidemis ble tatt for analyse av MDA ved hjelp av den tiobarbitursyrereaksjon som er nevnt i det foregående. Resultatene er angitt i det følgende og viser den beskyttende virkning av ekstrakten mot kutan skade som skyldes peroksydasjon utløst av en fotokjemisk reaksjon. The protective effect of the extract against this process can be demonstrated by a test where the extract, when diluted in a cosmetic aid and applied to the skin of an experimental animal, inhibits the formation of MDA which is normally induced by irradiation in the cutaneous tissue. A typical experiment is shown in the following: Hairless mice were sensitized by bathing for five minutes in a solution of Rose Bengal (10"<5>M) and then dried in the dark. The diluted extract or adjuvant was applied to the surface of the dorsal skin of the animals ten minutes before they were irradiated. The irradiation was carried out for 30 minutes using a 75 watt KRYPTON incandescent lamp placed at a distance of 25 cm from the animals. Control mice were kept in the dark. The animals were sacrificed and samples of their epidemi taken for analysis of MDA by means of the thiobarbituric acid reaction mentioned above.The results are given below and show the protective action of the extract against cutaneous damage due to peroxidation triggered by a photochemical reaction.
Det kosmetiske preparat kan ytterligere om ønsket inneholde hvilke som helst andre substanser som er kjent å ha gunstige egenskaper med hensyn til skjønnhetspleie, som kollagen, elastin, hyaluronsyre, lipidforbindelser inneholdende umettede fettsyrer, liposomer, fosforlipider, fuktemidler, vitaminekst-rakter, parfymer, konserveringsmidler, fargestoffer. De kan også inneholde solfiltere. The cosmetic preparation can further, if desired, contain any other substances known to have beneficial properties with regard to beauty care, such as collagen, elastin, hyaluronic acid, lipid compounds containing unsaturated fatty acids, liposomes, phospholipids, moisturisers, vitamin extracts, perfumes, preservatives , dyes. They can also contain solar filters.
Kosmetiske preparater kan gjøres tilgjengelige i alle former anvendt innen skjønnhetspleien, som kremer eller geler i krukker eller tuber, emulsjoner, lotioner i glass eller plastflasker, og om ønsket i glassdispensere eller endog ampuller eller spraypreparater. Cosmetic preparations can be made available in all forms used in beauty care, such as creams or gels in jars or tubes, emulsions, lotions in glass or plastic bottles, and if desired in glass dispensers or even ampoules or spray preparations.
For hvert preparat må man anvende passende hjelpestoffer. Disse hjelpestoffer må ha alle de egenskaper som vanligvis kreves, og eksempler som kan nevnes er glyserolstearat, propylenglykol, lanolin, glyserol, cetylalkohol, polyoler, vegetabilske, animalske og mineraloljer, fosfolipider som liposomer, voksarter, fuktemidler, bindemidler, stabilise-ringsmidler og emulgeringsmidler som vanlig anvendt. Appropriate excipients must be used for each preparation. These auxiliaries must have all the properties that are usually required, and examples that can be mentioned are glycerol stearate, propylene glycol, lanolin, glycerol, cetyl alcohol, polyols, vegetable, animal and mineral oils, phospholipids such as liposomes, waxes, humectants, binders, stabilizers and emulsifiers as commonly used.
De forskjellige kosmetiske former som nevnes i det foregående fremstilles ved hjelp av metoder som vanlig anvendt på området. The various cosmetic forms mentioned above are produced using methods commonly used in the field.
I det følgende gis eksempler på kosmetiske preparater (vekt-deler) fremstilt ved å anvende en ekstrakt av Cichorium intybus L inneholdende 1 mg/ml polyfenoler: The following are examples of cosmetic preparations (parts by weight) produced by using an extract of Cichorium intybus L containing 1 mg/ml polyphenols:
1. Olj e/vannkrem 1. Oil e/water cream
2. Vann/olj ekrem 3. Vandig alkoholisk lotion 2. Water/oil cream 3. Aqueous alcoholic lotion
Claims (1)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR8801464A FR2626469B1 (en) | 1988-01-29 | 1988-01-29 | NEW COSMETIC PREPARATIONS CONTAINING AN EXTRACT OF THE AERIAL PARTS OF CICHORIUM INTYBUS L |
| PCT/EP1989/000073 WO1989006954A1 (en) | 1988-01-29 | 1989-01-27 | Novel cosmetic compositions containing an extract of the aerial parts of cichorium intybus l |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| NO893815D0 NO893815D0 (en) | 1989-09-26 |
| NO893815L NO893815L (en) | 1989-09-26 |
| NO174915B true NO174915B (en) | 1994-04-25 |
| NO174915C NO174915C (en) | 1994-08-03 |
Family
ID=26069636
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO893815A NO174915C (en) | 1988-01-29 | 1989-09-26 | Cosmetic preparation |
Country Status (1)
| Country | Link |
|---|---|
| NO (1) | NO174915C (en) |
-
1989
- 1989-09-26 NO NO893815A patent/NO174915C/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| NO174915C (en) | 1994-08-03 |
| NO893815D0 (en) | 1989-09-26 |
| NO893815L (en) | 1989-09-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4992264A (en) | Novel cosmetic compositions containing an extract of the aerial parts of Cichorium intybus L | |
| KR100823533B1 (en) | Skin condition improvement composition containing alpha bisabolol as an active ingredient | |
| US4749573A (en) | Cosmetic preparations useful for opposing skin aging containing an extract of the fruits of Silybum marianum | |
| JP4201399B2 (en) | Skin aging prevention / improving agent | |
| KR20100033573A (en) | The composition for anti-oxidation or anti-inflammation comprising chrysanthemum extract and the method of preparation thereof | |
| US6096316A (en) | Composition for limiting and attenuating the visible symptoms of natural or accidental ageing of the skin | |
| JPH08175958A (en) | Topical skin | |
| KR20020080657A (en) | Cosmetics comprising Albizzia bark extracts for antioxidation | |
| JPH11171723A (en) | Antioxidant | |
| NO174915B (en) | Cosmetic preparation | |
| JPH08175954A (en) | Skin cosmetics | |
| KR100862969B1 (en) | Skin whitening agent containing barbituric acid as an active ingredient | |
| KR100795514B1 (en) | Skin whitening composition containing diosgenin | |
| KR20030011957A (en) | Cosmetic composition containing ginsan polysaccharide extracted from Panax ginseng | |
| KR100823537B1 (en) | Anti-obesity composition comprising alpha bisabolol as an active ingredient | |
| KR19990085303A (en) | Skin whitening material | |
| KR20220010919A (en) | Cosmetic composition for improving skin wrinkle comprising extract of crataegus pinnatifida fruit | |
| KR101791488B1 (en) | Composition of skin external application for alleviating stimulation caused from ultraviolet ray containing Oldenlandia Diffusa Willd extracts | |
| JPH08175955A (en) | Wrinkle formation inhibitor | |
| KR20010018663A (en) | A cosmetic composition containing Terminaliae Fructus extracts | |
| KR100823535B1 (en) | Hair loss prevention or hair growth promoting composition comprising alpha bisabolol as an active ingredient | |
| KR100845888B1 (en) | Skin condition improvement composition comprising brew branco oil as an active ingredient | |
| KR20230111466A (en) | Cosmetic Composion for Skin Whitening Comprising Fraction of Ginseng Flower Extract | |
| KR100875755B1 (en) | Skin condition improvement composition comprising leaf greens extract as the main active ingredient | |
| JPH1171291A (en) | Immunoactivator |