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NL9001056A - Cellulose prodn. from algae - in form of poly-beta-(1,4)glycan - Google Patents

Cellulose prodn. from algae - in form of poly-beta-(1,4)glycan Download PDF

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Publication number
NL9001056A
NL9001056A NL9001056A NL9001056A NL9001056A NL 9001056 A NL9001056 A NL 9001056A NL 9001056 A NL9001056 A NL 9001056A NL 9001056 A NL9001056 A NL 9001056A NL 9001056 A NL9001056 A NL 9001056A
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NL
Netherlands
Prior art keywords
cellulose
algae
micron
glycan
beta
Prior art date
Application number
NL9001056A
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Dutch (nl)
Original Assignee
Univ Amsterdam
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Univ Amsterdam filed Critical Univ Amsterdam
Priority to NL9001056A priority Critical patent/NL9001056A/en
Publication of NL9001056A publication Critical patent/NL9001056A/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/04Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds

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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A new method for obtaining cellulose uses algae as the source, esp. Crinalium epipsammum. This contains poly-beta-(1-4)glycan, a type of cellulose which is not present in crystalline form as in higher plants, but which crystallises out after mild acid treatment. Crinalium epipsammum forms short straight trichoma 5-7 micron wide and 1.5 micron thick with a cell length of 1.5 - 2 micron. The cell wall is notably thick at +/- 100 micron. The thickness and the amt. of N-acetyl-muramic acid (a component of peptide glycan) are comparable with those of Gram-positive bacteria.

Description

Werkwijze ter verkrijging van cellulose.Method for obtaining cellulose.

De uitvinding heeft betrekking op een nieuwe werkwijze ter verkrijging van cellulose.The invention relates to a new method for obtaining cellulose.

Het doel van de uitvinding is de verkrijging van een andere goede werkwijze voor het produceren van cellulose die gebruik maakt van algen. Het gebruik van algen is vooral aantrekkelijk omdat de algen gekweekt kunnen worden in grote vijvers. Deze vijvers kunnen gebruikt worden voor de verwerking van mest.The object of the invention is to obtain another good method for producing cellulose using algae. The use of algae is especially attractive because the algae can be grown in large ponds. These ponds can be used for manure processing.

Het doel wordt bereikt door gebruikmaking van algen in het bijzonder Crinalium epipsammum. Dit is een cyanobacterie die is geïsoleerd uit een algenkorst uit de binnenduinen van Meyendel bij Den Haag. Deze alg is inmiddels geregistreerd in het soortenregister voor algen te Göttingen onder nummer SAB 22.89..The object is achieved by using algae, in particular Crinalium epipsammum. This is a cyanobacterium isolated from an algae crust from the inner dunes of Meyendel near The Hague. This alga is now registered in the species register for algae in Göttingen under number SAB 22.89.

Crinalium epipsammum vormt korte rechte trichomen welke een afgeplatte vorm hebben. De trichomen versmallen niet naar het einde toe. Vertakkingen zijn niet gevonden. De breedte van de trichomen is 5-7 μ, de dikte 1,5 μ en de celllengte is 1,5-2 μ. De lengte van de trichomen kan variëren afhankelijk van de kultuurcondities. De geïsoleerde stam vertoonde geen celdifferentiatie en hormogoniën worden eveneens niet gevormd. De trichomen zijn niet beweeglijk. De celdeling is volgens het Oscillatoria-type.Crinalium epipsammum forms short straight trichomes that have a flattened shape. The trichomes do not narrow towards the end. Branches were not found. The width of the trichomes is 5-7 μ, the thickness 1.5 μ, and the cell length is 1.5-2 μ. The length of the trichomes can vary depending on the culture conditions. The isolated strain showed no cell differentiation and hormogonias are not formed either. The trichomes are not mobile. The cell division is according to the Oscillatoria type.

Met behulp van transmissie electron mikroscopische opnamen is de ultrastruktuur onderzocht. Hierbij is gebleken dat de celwand opvallend dik is gebleken: ± 100 nm. De dikte en de hoeveelheid N-acetyl-muramine zuur, een component van peptide glycaan, is vergelijkbaar met die van Gram-positieve bacteriën.The ultrastructure was examined with the aid of transmission electron microscopic images. It has been found that the cell wall has turned out to be remarkably thick: ± 100 nm. The thickness and amount of N-acetyl-muramic acid, a component of peptide glycan, is comparable to that of Gram-positive bacteria.

Nieuw is de aanwezigheid van poly-ß-(l-4)glycaan, een type cellulose. Dit is de eerste keer dat bij cyanobacteriën dit polymeer is aangetroffen. Het cellulose komt niet in de kristallijnige vorm voor zoals bij hogere planten, maar kristalliseert uit na milde zuurbehandeling. In de Verenigde Staten is men in een vergevorderd stadium met de ontwikkeling tot verwerking van dit soort cellulose.New is the presence of poly-ß- (1-4) glycan, a type of cellulose. This is the first time that this polymer has been found in cyanobacteria. The cellulose does not exist in the crystalline form as in higher plants, but crystallizes after mild acid treatment. The United States is at an advanced stage of development into the processing of this type of cellulose.

In de figuur is een afbeelding te zien van Crinalium epipsammum gekweekt in continu cultuur waarin A de lengtedoorsnede van de trichoom weergeeft en B de dwarsdoorsnede van de trichoom weergeeft. De schaalstreep representeert 7 μ.The figure shows an image of Crinalium epipsammum grown in continuous culture in which A represents the longitudinal section of the trichome and B represents the cross-section of the trichome. The scale bar represents 7 μ.

Claims (4)

1. Werkwijze ter verkrijging van cellulose met het kenmerk dat er gebruik wordt gemaakt van algen.Method of obtaining cellulose, characterized in that use is made of algae. 2. Werkwijze volgens conclusie 1. met het kenmerk dat de alg Crinalium epipsammum wordt gebruikt.Method according to claim 1, characterized in that the algae Crinalium epipsammum is used. 3. Werkwijze volgens één van de voorgaande conclusies met het kenmerk dat de algen worden gekweekt in grote vijvers.Method according to one of the preceding claims, characterized in that the algae are grown in large ponds. 4. Werkwijze volgens de voorgaande conclusie met het kenmerk dat de vijvers worden gebruikt voor de verwerking van mest.Method according to the preceding claim, characterized in that the ponds are used for processing manure.
NL9001056A 1990-05-02 1990-05-02 Cellulose prodn. from algae - in form of poly-beta-(1,4)glycan NL9001056A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
NL9001056A NL9001056A (en) 1990-05-02 1990-05-02 Cellulose prodn. from algae - in form of poly-beta-(1,4)glycan

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NL9001056 1990-05-02
NL9001056A NL9001056A (en) 1990-05-02 1990-05-02 Cellulose prodn. from algae - in form of poly-beta-(1,4)glycan

Publications (1)

Publication Number Publication Date
NL9001056A true NL9001056A (en) 1991-12-02

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NL9001056A NL9001056A (en) 1990-05-02 1990-05-02 Cellulose prodn. from algae - in form of poly-beta-(1,4)glycan

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8574400B1 (en) 2012-05-25 2013-11-05 Kimberly-Clark Worldwide, Inc. Tissue comprising macroalgae
US9074324B2 (en) 2013-06-10 2015-07-07 Kimberly-Clark Worldwide, Inc. Layered tissue structures comprising macroalgae
US9499941B2 (en) 2012-05-25 2016-11-22 Kimberly-Clark Worldwide, Inc. High strength macroalgae pulps

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8574400B1 (en) 2012-05-25 2013-11-05 Kimberly-Clark Worldwide, Inc. Tissue comprising macroalgae
US8771468B2 (en) 2012-05-25 2014-07-08 Kimberly-Clark Worldwide, Inc. Tissue comprising macroalgae
US9499941B2 (en) 2012-05-25 2016-11-22 Kimberly-Clark Worldwide, Inc. High strength macroalgae pulps
US9074324B2 (en) 2013-06-10 2015-07-07 Kimberly-Clark Worldwide, Inc. Layered tissue structures comprising macroalgae

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