NL2037632A - Microanalyzer based on self-compensated near infrared spr effect - Google Patents
Microanalyzer based on self-compensated near infrared spr effect Download PDFInfo
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- 230000000694 effects Effects 0.000 title claims abstract description 45
- 238000001252 near-infrared surface plasmon resonance spectroscopy Methods 0.000 title claims abstract description 15
- 238000001514 detection method Methods 0.000 claims abstract description 74
- 238000012545 processing Methods 0.000 claims abstract description 20
- 230000005284 excitation Effects 0.000 claims abstract description 18
- 238000012360 testing method Methods 0.000 claims description 31
- 239000013307 optical fiber Substances 0.000 claims description 27
- 239000012528 membrane Substances 0.000 claims description 25
- 239000011521 glass Substances 0.000 claims description 18
- 230000003287 optical effect Effects 0.000 claims description 16
- 238000011895 specific detection Methods 0.000 claims description 13
- 238000005192 partition Methods 0.000 claims description 12
- 230000005540 biological transmission Effects 0.000 claims description 10
- 239000000835 fiber Substances 0.000 claims 6
- 239000012530 fluid Substances 0.000 claims 1
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 description 46
- 238000010586 diagram Methods 0.000 description 10
- 230000035945 sensitivity Effects 0.000 description 9
- JBRZTFJDHDCESZ-UHFFFAOYSA-N AsGa Chemical compound [As]#[Ga] JBRZTFJDHDCESZ-UHFFFAOYSA-N 0.000 description 6
- 229910052738 indium Inorganic materials 0.000 description 6
- APFVFJFRJDLVQX-UHFFFAOYSA-N indium atom Chemical compound [In] APFVFJFRJDLVQX-UHFFFAOYSA-N 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 3
- 239000002120 nanofilm Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 230000017525 heat dissipation Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000007877 drug screening Methods 0.000 description 1
- 239000004973 liquid crystal related substance Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
- G01N21/552—Attenuated total reflection
- G01N21/553—Attenuated total reflection and using surface plasmons
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
- G01N21/4133—Refractometers, e.g. differential
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- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
Disclosed is a microanalyzer based on a self- compensated near infrared SPR effect. The microanalyzer includes a laser emitting module, a light intensity self- compensation module, a multi-channel detection module, a 5 SPR excitation module, a multi-channel photoelectric detection module, a signal processing module and a result display module. The laser emitting module emits C-band laser, and the light intensity self-compensation module divides the C-band laser into s-polarized light which 10 cannot excite an SPR effect and p-polarized light which can excite the SPR effect. The multi-channel detection module simultaneously detects a plurality of samples to be detected, and the SPR excitation module generates multi- channel reflected light with the SPR effect. The multi- 15 channel photoelectric detection module detects light intensity signals of the s-polarized light and the multi- channel reflected light, and the signal processing module determines refractive indexes and concentrations of the samples to be detected according to the light intensity 20 signals.
Description
MICROANALYZER BASED ON SELF-COMPENSATED NEAR INFRARED SPR
[01] The present invention relates to the technical field of SPR sensing, in particular to a microanalyzer based on a self-compensated near infrared SPR effect.
[02] SPR (Surface Plasmon Resonance) technology is an important biological analysis technology, which can detect the interaction between biomolecules in real time. SPR technology is based on the adsorption of biomolecules on metal surface, and uses the change of reflected light intensity caused by surface plasma wave generated by laser irradiation to detect the interaction between biomolecules, which is widely used in drug screening, biosensing, biomedical research and other fields.
[03] At present, high-performance SPR analyzers on the market are generally large in size and high in cost, which limits the popularity of SPR analyzers.
[04] An objective of the present invention is to provide a microanalyzer based on a self-compensated near infrared
SPR effect, so as to reduce a cost and volume of the instrument while realizing high sensitivity, high precision and rapid detection.
[05] In order to achieve the above objective, the present invention provides the following solution:
[06] The microanalyzer based on a self-compensated near infrared SPR effect includes a laser emitting module, a light intensity self-compensation module, a multi-channel detection module, a SPR excitation module, a multi-channel photoelectric detection module, a signal processing module and a result display module.
[07] The laser emitting module is configured to emit C- band laser, and make the C-band laser be incident on the light intensity self-compensation module.
[08] The light intensity self-compensation module is configured to divide the C-band laser into s-polarized light which may not excite an SPR effect and p-polarized light which may excite the SPR effect, and make the p- polarized light be incident on the SPR excitation module and the s-polarized light be incident on the multi-channel photoelectric detection module.
[09] The multi-channel detection module includes a plurality of sample test channels and a non-specific reference channel, and is configured to simultaneously detect a plurality of samples to be detected.
[10] The SPR excitation module excites the SPR effect of the samples to be detected with the p-polarized light and generates multi-channel reflected light with the SPR effect, and makes the multi-channel reflected light be incident on the multi-channel photoelectric detection module.
[11] The multi-channel photoelectric detection module is configured to detect light intensity signals of the s- polarized light and the multi-channel reflected light, and send the light intensity signals to the signal processing module.
[12] The signal processing module is configured to determine refractive indexes and concentrations of the samples to be detected according to the light intensity signals, and send the refractive indexes and the concentrations to the result display module.
[13] The result display module is configured to display the refractive indexes and the concentrations.
[14] Alternatively, the laser emitting module includes an optical fiber laser emitter, a transmission optical fiber, a C-band laser diode, an incident light optical fiber collimating lens and a collimating lens bracket.
[15] The optical fiber laser transmitter is connected to the C-band laser diode by means of the transmission optical fiber, and the optical fiber laser transmitter is configured to generate laser and make the laser be incident on the C-band laser diode by means of the transmission optical fiber, and generate the C-band laser by the C-band laser diode.
[16] The incident light optical fiber collimating lens is mounted on the collimating lens bracket, the collimating lens bracket is configured to fix the incident light optical fiber collimating lens on an optical path of the
C-band laser incident on the light intensity self- compensation module, and the incident light optical fiber collimating lens is configured to collimate the C-band laser and expand beam diameter.
[17] Alternatively, the light intensity self-compensation module includes a polarizing beam splitter and a polarizing beam splitter bracket.
[18] The polarizing beam splitter is mounted in the polarizing beam splitter bracket, and the polarizing beam splitter bracket matches and is connected to the collimating lens bracket by means of threads.
[19] The polarizing beam splitter divides the C-band laser into the s-polarized light which does not excite the
SPR effect and the p-polarized light which excites the SPR effect.
[20] Alternatively, the SPR excitation module includes a semi-cylindrical prism bracket, a semi-cylindrical prism and a sensing chip, and the sensing chip includes a sensing membrane and a glass sheet.
[21] The semi-cylindrical prism is mounted in the semi- cylindrical prism bracket, and the semi-cylindrical prism bracket matches and is connected to the polarizing beam splitter bracket.
[22] The glass sheet is located on a plane of the semi- cylindrical prism, and the semi-cylindrical prism and the glass sheet are coupled by a refractive index matching liquid; and the sensing membrane is arranged on the glass sheet.
[23] Alternatively, the p-polarized light is obliquely incident on the sensing chip at a fixed angle of 62.77 degrees -62.8 degrees.
[24] Alternatively, the multi-channel detection module includes a cover plate arranged on a housing, hoses and hose plugs.
[25] The cover plate is located at a top of the housing, and a plurality of partition regions are arranged on the cover plate; and the hose plugs are provided with threads, and the hoses are fixed on the partition regions of the cover plate by means of the hose plugs.
[26] The sensing chip is located directly below the cover plate, the partition regions of the cover plate correspond to multiple channels on the sensing chip, and the multiple channels include the plurality of sample test channels and the non-specific reference channel; each sample test channel is filled with different specific detection molecules by means of the corresponding hoses to modify the sensing chip; and the non-specific reference channel corresponds to the sensing membrane which is not modified with the specific detection molecules.
[27] Alternatively, the multi-channel photoelectric detection module includes a light intensity reference channel detector, a plurality of sample test channel detectors and a non-specific reference channel detector.
[28] The light intensity reference channel detector is mounted in the polarizing beam splitter bracket and located on an emergent optical path of the s-polarized light, and is configured to detect the light intensity signal of the s-polarized light.
[29] The plurality of sample test channel detectors and the non-specific reference channel detector are mounted on the semi-cylindrical prism bracket and located on an emergent optical path of the multi-channel reflected light.
[30] Alternatively, the signal processing module includes a multi-channel signal converter, a single chip microcomputer expansion board and a single chip microcomputer which are connected in sequence. 5 [31] The multi-channel signal converter 1s connected to the multi-channel photoelectric detection module and configured to convert the multi-channel light intensity signals into multi-channel electric signals; and the multi-channel electric signals are transmitted to the single chip microcomputer by means of the single chip microcomputer expansion board.
[32] The single chip microcomputer is configured to calculate the refractive indexes and the concentrations of the plurality of samples to be detected according to the multi-channel electrical signals.
[33] Alternatively, the result display module includes a display screen.
[34] The display screen is located at the top of the housing and connected to the single chip microcomputer, and is configured to display the refractive indexes and the concentrations of the plurality of samples to be detected.
[35] Alternatively, the microanalyzer further includes: a power supply, a charging port and a power switch.
[36] The power supply device is located inside the housing, and the charging port and the power switch are located at one side of the housing; and the power supply is connected to an external power supply by means of the charging port for charging, and the power supply is connected to the laser emitting module, the multi-channel photoelectric detection module, the signal processing module and the result display module by means of the power switch to supply power separately.
[37] According to the specific examples provided by the present invention, the present invention discloses the following technical effects:
[38] The microanalyzer based on a self-compensated near infrared SPR effect is provided by the present invention and includes the laser emitting module, the light intensity self-compensation module, the multi-channel detection module, the SPR excitation module, the multi- channel photoelectric detection module, the signal processing module and the result display module. The laser emitting module is configured to emit the C-band laser, and make the C-band laser be incident on the light intensity self-compensation module. The light intensity self-compensation module is configured to divide the C- band laser into the s-polarized light which may not excite the SPR effect and the p-polarized light which may excite the SPR effect, and make the p-polarized light be incident on the SPR excitation module and the s-polarized light be incident on the multi-channel photoelectric detection module. The multi-channel detection module includes the plurality of sample test channels and the non-specific reference channel, and is configured to simultaneously detect the plurality of samples to be detected. The SPR excitation module excites the SPR effect of the samples to be detected with the p-polarized light and generates the multi-channel reflected light with the SPR effect, and makes the multi-channel reflected light be incident on the multi-channel photoelectric detection module. The multi- channel photoelectric detection module is configured to detect the light intensity signals of the s-polarized light and the multi-channel reflected light, and send the light intensity signals to the signal processing module.
The signal processing module is configured to determine the refractive indexes and the concentrations of the samples to be detected according to the light intensity signals, and send the refractive indexes and the concentrations to the result display module. The result display module is configured to display the refractive indexes and the concentrations. The microanalyzer based on a self-conpensated near infrared SPR effect provided by
: the present invention realizes detection of the concentrations of the samples to be detected based on the near infrared SPR effect, and compared with an existing detection device using a spectrometer, device volume is reduced on the basis of ensuring sensing performance. In addition, the present invention provides the plurality of test channels, which may simultaneously detect a plurality of objects to be detected. The self-compensation module and the reference channel arranged in the present invention greatly improve sensitivity and stability of detection.
[39] In order to describe the examples of the present invention or the technical solutions in the prior art clearer, and the accompanying drawings required by the examples are briefly described below. Obviously, the accompanying drawings in the following description show merely some examples of the present invention, and a person of ordinary skill in the art would also be able to derive other accompanying drawings from these accompanying drawings without creative efforts.
[40] FIG. 1 is an overall schematic structural diagram of a microanalyzer provided by the present invention;
[41] FIG. 2 is a schematic diagram of front and back of a sensing detection area and an enlarged schematic diagram of a sensing membrane area of the microanalyzer provided by the present invention;
[42] FIG. 3 is a schematic structural diagram of an external part of the microanalyzer provided by the present invention; and
[43] FIG. 4 is a schematic diagram showing size of a housing of the microanalyzer provided by the present invention.
[44] The technical solution in the examples of the present invention is clearly and completely described below with reference to the accompanying drawings in the examples of the present invention. Apparently, the described examples are some rather than all of the examples of the present invention. Based on the examples of the present disclosure, all the other examples obtained by those of ordinary skill in the art without inventive effort are within the scope of protection of the present invention.
[45] At present, high-performance SPR analyzers on the market are generally large in size and high in cost, which limits popularity of the SPR analyzers to some extent.
Therefore, it is of great significance to research and develop an SPR analyzer with miniaturization and high sensitivity, which may effectively reduce a cost and volume of the instrument while realizing high sensitivity, high precision and rapid detection. An objective of the present invention is to provide a microanalyzer based on a self-compensated near infrared SPR effect, so as to reduce the cost and the volume of the instrument while realizing the high sensitivity, high precision and rapid detection.
The microanalyzer may be used for rapid and precision detection of biomolecules, and has the advantages of a low cost, miniaturization, high precision, convenient usage, etc.
[46] In order to make the above objective, features and advantages of the present disclosure clearer and more comprehensible, the present disclosure will be further described below in detail with reference to the accompanying drawings and particular embodiments.
[47] FIG. 1 is an overall schematic structural diagram of the microanalyzer based on a self-compensated near infrared SPR effect provided by the present invention, and
FIG. 2 is a schematic diagram of front and back of a sensing detection area and an enlarged schematic diagram of a sensing membrane area of the microanalyzer provided by the present invention. As shown in FIG. 1 and FIG. 2, the microanalyzer based on a self-compensated near infrared SPR effect includes a laser emitting module, a light intensity self-compensation module, a multi-channel detection module, a SPR excitation module, a multi-channel photoelectric detection module, a signal processing module and a result display module.
[48] The laser emitting module is configured to emit C- band laser, and make the C-band laser be incident on the light intensity self-compensation module.
[49] Specifically, the laser emitting module includes an optical fiber laser emitter 1, a transmission optical fiber 22, a C-band laser diode 2, an incident light optical fiber collimating lens 3 and a collimating lens bracket 19.
[50] As shown in FIG. 1 and FIG. 2, the optical fiber laser transmitter 1 is connected to the C-band laser diode 2 by means of the transmission optical fiber 22. The optical fiber laser transmitter 1 supplies power to the C- band laser diode 2 to generate laser and adjust laser intensity, the generated laser is incident on the C-band laser diode 2 by means of the transmission optical fiber 22, and the C-band laser diode 2 generates the C-band laser.
[51] The incident light optical fiber collimating lens 3 is mounted on the collimating lens bracket 19, the incident light optical fiber collimating lens 3 matches and is connected to the collimating lens bracket 19 by means of threads, and the collimating lens bracket 19 is configured to fix the incident light optical fiber collimating lens 3 on an optical path of the C-band laser incident on the light intensity self-compensation module, collimate the C-band laser and expand beam diameter, such that the whole sensing chip may be covered with the C-band laser,
[52] The light intensity self-compensation module is configured to divide the C-band laser into s-polarized light which may not excite an SPR effect and p-polarized light which may excite the SPR effect, and make the p- polarized light be incident on the SPR excitation module and the s-polarized light be incident on the multi-channel photoelectric detection module.
[53] Specifically, the light intensity self-compensation module includes a polarizing beam splitter 4 and a polarizing beam splitter bracket 18. The polarizing beam splitter 4 is mounted in the polarizing beam splitter bracket 18, and the polarizing beam splitter bracket 18 matches and is connected to the collimating lens bracket 19 by means of threads.
[54] The polarizing beam splitter 4 divides the C-band laser into the s-polarized light which may not excite the
SPR effect and the p-polarized light which may excite the
SPR effect. The s-polarized light provides a reference signal for a detection signal to correct errors caused by light source fluctuation. With reference to light intensity, fluctuation, etc. of the s-polarized light, the p-polarized light which excites the SPR effect may be processed accordingly, which may reduce an error of the molecular detection signal caused by light source noise, chip preparation technology. non-specific adsorption, etc.
[55] Specifically, the multi-channel detection module includes a plurality of sample test channels and a non- specific reference channel, and is configured to simultaneously detect a plurality of samples to be detected. Sensing channels of the present invention are divide into two types: one is the multi-channel sample test channels, and the other is the non-specific reference channel. In order to detect the plurality of samples to be detected at the same time, it is necessary to arrange the multi-channel sample test channels. Before usage, each sample test channel is filled with different specific detection molecules to modify the sensing chip; and the non-specific reference channel corresponds to the sensing chip without modifying a metal sensing membrane with a specific detection molecular film. The sensing chip of the multi-channel sample test channels and the non-specific reference channel has the same material, gold membrane thickness and other parameters except the specific detection molecular film.
[56] With reference to FIG. 1 and FIG. 3, the multi- channel detection module includes a cover plate 17 arranged on a housing 13, hoses 24 and hose plugs 25. The cover plate 17 is located at a top of the housing 13, and a plurality of partition regions are provided on the cover plate 17 (three partition regions are shown in FIG. 3).
The partition regions on the cover plate 17 divides the sensing membrane 7 into a plurality of sample test channels and the non-specific reference channel, which are configured to simultaneously detect the plurality of samples to be detected and provide the reference signal for the detection signal to correct results.
[57] The hose plugs 25 are provided with threads, and the hoses 24 are fixed on the partition regions of the cover plate 17 by means of the hose plugs 25. The sensing chip is located directly below the cover plate 17, the partition regions of the cover plate 17 correspond to multiple channels on the sensing chip, and the multiple channels include the plurality of sample test channels and the non-specific reference channel. Each sample test channel is filled with different specific detection molecules by means of the corresponding hoses 24 to modify the sensing chip. The non-specific reference channel corresponds to the sensing membrane which is not modified with the specific detection molecules.
[58] Specifically, the SPR excitation module excites the
SPR effect of the samples to be detected with the p- polarized light and generates multi-channel reflected light with the SPR effect, and makes the multi-channel reflected light be incident on the multi-channel photoelectric detection module.
[59] With reference to FIG. 1 and FIG. 2, the SPR excitation module includes a semi-cylindrical prism bracket 23, a semi-cylindrical prism 5 and the sensing chip. The sensing chip includes the sensing membrane 7 and a glass sheet 6. In a specific example, a K9 semi- cylindrical prism is preferably used as the semi- cylindrical prism 5, and a K9 glass sheet is preferably used as the glass sheet 6.
[60] The semi-cylindrical prism 5 is mounted in the semi- cylindrical prism bracket 23, and the semi-cylindrical prism bracket 23 matches and is connected to the polarizing beam splitter bracket 18. The glass sheet 6 is located on a plane of the semi-cylindrical prism 5, and the semi-cylindrical prism 5 and the glass sheet 6 are coupled by refractive index matching liquid; and the sensing membrane 7 is placed on the glass sheet 6. The glass sheet 6 is detachable, the material and thickness of the sensing membrane 7 are determined by physical and chemical means according to the type of the sample to be tested, and the sensing membrane 7 is modified on the glass sheet 6. For different detection substances, a corresponding biomolecular membrane system is modified, and different samples to be detected are detected by replacing the corresponding sensing membrane 7 when the cover plate 17 on the top of the housing 13 is opened. For example, a gold membrane having a thickness of 50 nm is the sensing membrane 7 commonly used in a detection device.
[61] The p-polarized light which may excite the SPR effect is incident on the semi-cylindrical prism 5 and the sensing chip in sequence, and the p-polarized light covers the sensing chip of the plurality of channels and excites the near infrared SPR effect, such that an SPR evanescent field is radiated to the samples to be detected in a near field of the sensing chip, and then the multi-channel reflected light with SPR absorption characteristics and refractive index information of the samples to be detected passes through the sensing chip and the semi-cylindrical prism 5 in sequence and is detected by a plurality of indium gallium arsenic photoelectric detectors.
[62] As an example, the p-polarized light is obliquely incident on the sensing chip at a fixed angle of 62.77 degrees -62.8 degrees. An incident angle of the p- polarized light determines resonant wavelength, setting the incident angle to be 62.77 degrees -62.8 degrees makes the resonant wavelength slightly larger than wavelength of the optical fiber laser transmitter, and sensitivity is optimal.
[63] Specifically, the multi-channel photoelectric detection module is configured to detect light intensity signals of the s-polarized light and the multi-channel reflected light, and send the light intensity signals to the signal processing module.
[64] The multi-channel photoelectric detection module of the present invention may detect the multi-channel light intensity signals, and the multi-channel light intensity signals include the light intensity signals of the light intensity self-compensation module and the multi-channel light intensity signals of the SPR excitation module. The multi-channel photoelectric detection module includes a plurality of indium gallium arsenic photoelectric detectors, each indium gallium arsenic photoelectric detector includes a light intensity reference channel detector 801, a plurality of sample test channel detectors and a non-specific reference channel detector 804. The light intensity reference channel detector 801 is mounted in the polarizing beam splitter bracket 18 and located on an emergent optical path of the s-polarized light, and is configured to detect the light intensity signal of the s- polarized light. The plurality of sample test channel detectors and the non-specific reference channel detector 804 are mounted on the semi-cylindrical prism bracket 23 and located on an emergent optical path of the multi- channel reflected light.
[65] As a specific example, the partition regions on the cover plate 17 divide the sensing membrane 7 into two sample test channels (a sample A test channel and a sample
B test channel) and a non-specific reference channel, which are three channels in total. According to the present invention, firstly, a non-specific sensing membrane is modified on the glass sheet 6: class A specific detection molecules are introduced into the sample A test channel, a class B specific detection molecular membrane is introduced into the sample B test channel, and the non-specific reference channel corresponds to the sensing membrane without the specific detection molecular film. If a plurality of samples A and
B to be detected are dissolved in the same sample to be detected, the samples to be detected may be simultaneously introduced into all channels. The sample A test channel detector 802, the sample B test channel detector 803 and the non-specific reference channel detector 804 are correspondingly arranged at the sample A test channel, the sample B test channel and the non-specific reference channel, and configured to detect and compare the light intensity signals of the reflected light of the channels, the light intensity signal obtained by the non-specific reference channel detector 804 is taken as a control signal, and concentrations of the sample A and the sample
B to be detected may be obtained.
[66] Specifically, the signal processing module is configured to determine refractive indexes and concentrations of the samples to be detected according to the light intensity signals, and send the refractive indexes and the concentrations to the result display module.
[67] With reference to FIG. 1 and FIG. 2, the signal processing module includes a multi-channel signal converter 9, a single chip microcomputer expansion board
20 and a single chip microcomputer 10 which are connected in sequence. The multi-channel signal converter 9 is connected to the multi-channel photoelectric detection module, that is, the multi-channel signal converter 9 is connected to the plurality of indium gallium arsenic photoelectric detectors by means of wires, and configured to convert the multi-channel light intensity signals into multi-channel electrical signals. The multi-channel electrical signals are transmitted to the single chip microcomputer 10 by means of the single chip microcomputer expansion board 20, that is, the multi-channel signal converter 9 is connected to the single chip microcomputer 10 and the single chip microcomputer expansion board 20 by means of wires. The multi-channel signal converter 3 converts the multi-channel light intensity signals into multi-channel current signals, and the single chip microcomputer expansion board 20 converts the current signals into voltage signals and transmits the voltage signals to the single chip microcomputer 10. The single chip microcomputer 10 is connected to the single chip microcomputer expansion board 20 by means of pins, and the single chip microcomputer expansion board 20 is connected to the multi-channel signal converter 9 by means of wires to receive light intensity information collected by the plurality of indium gallium arsenic photoelectric detectors in real time. The single chip microcomputer 10 is configured to determine the corresponding light intensity information according to the multi-channel voltage signals, and calculate the refractive indexes and the concentrations of the plurality of samples to be detected by means of the light intensity information.
[68] Specifically, the result display module is configured to display the refractive indexes and the concentrations.
[69] FIG. 3 is a schematic structural diagram of an external part of the microanalyzer provided by the present invention, as shown in FIG. 3, the result display module includes a display screen 11; and the display screen 11 is a serial liquid crystal display. The display screen 11 is located at the top of the housing 13 and connected to the single chip microcomputer 10, and is configured to display the refractive indexes and the concentrations of the plurality of samples to be detected.
[70] As shown in FIG. 3, the housing 13 of the microanalyzer based on a self-compensated near infrared
SPR effect provided by the present invention is provided with a heat dissipation hole 14, a power switch 15, a charging port 16, a USB port 21, the cover plate 17 and the display screen 11, the USB port 21, the cover plate 17 and the display screen 11 are located at the top of the housing 13, the heat dissipation hole 14 is located at a front side of the housing 13, and the charging port 16 and the power switch 15 are located at one side of the housing 13.
[71] The optical fiber laser transmitter 1, the power supply 12, the multi-channel signal converter 9 and the single chip microcomputer expansion board 20 are arranged inside the microanalyzer, and are connected and fixed to the housing 13 by means of screws separately. The power supply 12 is connected to an external power supply by means of the charging port 16 for charging, and the power supply 12 is connected to the laser emitting module, the multi-channel photoelectric detection module, the signal processing module and the result display module by means of the power switch 15 to supply power separately. The single chip microcomputer 10 transmits the calculated refractive indexes and concentration data of the samples to be detected to the display screen 11, and the data are saved to a storage device of a user by means of the USB port 21,
[72] As an example, the present invention uses a connecting bracket to fix all optical elements, and uses the transmission optical fiber 22 for optical path connection, and the optical elements are integrated into an internal structure of the instrument.
[73] FIG. 4 is a schematic diagram showing size of a housing of the microanalyzer provided by the present invention, and as shown in FIG. 4, the housing 13 of the microanalyzer has a length of a = 12 cm, a width of bh = 7 cm and a height of ¢ = 8 cm. Compared with an existing high-precision detection instrument, the present invention effectively reduces volume of the instrument, and realizes high precision and miniaturization.
[74] When the microanalyzer based on a self-compensated near infrared SPR effect provided by the present invention is used for detecting the samples to be detected, the specific use steps are as follows:
[75] 1) turning on the power switch 15, and turning on the microanalyzer.
[76] 2) Opening the cover plate 17 at the top of the housing 13, dropping a drop of refractive index matching liquid on the semi-cylindrical prism 5, and placing the glass sheet 6 coated with non-specific sensing membrane on the semi-cylindrical prism 5.
[77] 3) Closing the cover plate 17 at the top of the housing 13, and introducing specific detection substances corresponding to the samples to be detected into the sensing chip by means of the hoses 24 on the cover plate 17.
[78] 4) Dropping the samples to be detected on the sensing chip, or introducing the samples to be detected on the sensing chip by means of the hoses 24,
[79] 5) Clicking an OK button on the display screen 11, and making the display screen 11 display the refractive indexes and the concentrations of the samples to be detected.
[80] 6) Inserting a USB flash disk into the USB port 21 to store data.
[81] 7) After usage, turning off the power switch 15, and taking out the sensing chip for next usage.
[82] In summary, compared with the existing detection instrument in the market, the microanalyzer provided by the present invention has the following advantages:
[83] {1} Compared with the detection instrument with higher detection precision in the prior art, the microanalyzer of the present invention uses a near infrared light source, and the used laser wavelength is in a C band to excite the near infrared SPR effect, such that detection sensitivity is improved, detection precision is greatly enhanced, and on-line real-time detection is realized.
[84] {2) The microanalyzer of the present invention adds the polarizing beam splitter 4, which greatly reduces half-peak width of incident light, and improves a quality factor of a sensor.
[85] (3) The microanalyzer of the present invention uses the indium gallium arsenic photoelectric detectors instead of the spectrometer, the single chip microcomputer 10 instead of a computer, the small display screen 11 instead of a traditional display screen, and a charging module instead of an on-line power supply, such that volume of optical devices is reduced, portability and miniaturization of SPR sensing apparatus are realized, and a detection cost is greatly reduced.
[86] (4) Compared with an intensity modulation type SPR device in the prior art, the microanalyzer of the present invention uses the self-compensation module and the multi- channel detection module to realize self-compensation of stability of the light source, such that the detection sensitivity is improved.
[87] (5) The microanalyzer of the present invention may detect different samples to be detected at the same time with the multi-channel detection module, and the light intensity signal of the reference channel may be used as the reference signal to correct a signal error, such that a time cost is saved, and stability and sensitivity of the intensity modulation type SPR apparatus are greatly enhanced.
[88] (6) The microanalyzer of the present invention uses the semi-cylindrical prism 5, which reduces the volume of the instrument and a cost for correcting an incident light angle.
[89] Each example in the specification is described in a progressive manner, each example focuses on differences with another example, and the examples may refer to one another for the same and similar portions.
[90] The principles and embodiments of the present disclosure are described by applying specific examples in the present disclosure, and the description of the above examples is merely used for assisting in understanding the method and the core ideas of the present disclosure.
Moreover, based on the concept of the present disclosure, a person of ordinary skill in the art will make amendments to the particular embodiments and the application scope.
In conclusion, the content of the description should not be interpreted as limiting the present disclosure.
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| US20160313246A1 (en) * | 2013-11-04 | 2016-10-27 | Agency For Science, Technology And Research | Optical sensing device for surface plasmon resonance (spr) and optical sensing method using surface plasmon resonance (spr) |
| US20210190681A1 (en) * | 2017-11-10 | 2021-06-24 | Yokogawa Electric Corporation | Spectroscopic analysis device |
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| US20210190681A1 (en) * | 2017-11-10 | 2021-06-24 | Yokogawa Electric Corporation | Spectroscopic analysis device |
| CN115524312A (en) * | 2022-10-17 | 2022-12-27 | 大连海事大学 | A portable near-infrared SPR detection device |
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