MXPA06008494A - Natural tea having effects of hangover relief and liver function recovery and method for preparing the same - Google Patents

Abstract

A natural tea has effects of relieving hangovers and revitalizing liver functions, contains an extract from natural plant Robinia pseudo-acacia as a main active ingredient, and is obtained under optimal extraction conditions. The natural tea comprises an extract from the leaf, stem, flower and root of Robinia pseudo-acacia as a main active ingredient, and conventional additives, and optionally, at least one selected from Hedyotis diffusa, Amomi semen, Glycyrrhiza and Pueraiae Flos, as a minor active ingredient. The present invention provides the powder or liquid natural tea comprising Robinia pseudo-acacia extract as a main active ingredient, and optionally, a given amount of at least one selected from Hedyotis diffusa, Amomi semen, Glycyrrhiza and Pueraiae Flos extracts, as well as the method for preparing the same. The natural tea of the present invention shows the effects of relieving hangovers and protecting liver when taken before or after drinking alcohol.

Description

NATURAL TEA THAT HAS RELEASE EFFECTS OF THE SURFACE AND RECOVERY OF THE FUNCTION OF THE LIVER AND METHOD OF PREPARATION OF THE SAME Field of the Invention The present invention relates to a type of natural tea that has effects of release of the hangovers and revitalization of liver functions and a method to prepare it. More particularly, the present invention relates to a natural tea that has effects of relieving the hangover and revitalizing liver functions, which contains an extract of pseudo-acacia Robinia as the main active ingredient, which is obtained under conditions optimal extraction, as well as a method to prepare it. BACKGROUND OF THE INVENTION Generally, since the recorded beginning of human history, alcoholic beverages have appeared and are still the favorite beverages. However, these alcoholic beverages can cause several problems and among these problems, the most frequently mentioned are the health problems caused by drinking alcohol. That is, it is known that alcoholic beverages cause problems, such as liver disease and reduced functions of the stomach, colon and brain and the occurrence of such alcohol damage has continued to this day. Therefore, there have been ongoing investigations to protect the liver from alcohol and to relieve the hangovers caused by drinking it. As used in the present description, the term "hangover" refers to various symptoms, such as discomfort, headache and decreased mental and physical ability to work, which occur when it becomes sober and can last until the next day or up to two days after drinking alcohol. Such hangovers seem to occur because alcohol or acetaldehyde is accumulated in the human body due to lack of alcohol dehydrogenase or acetaldehyde dehydrogenase. In other words, when alcohol is ingested in the body by taking it, it is degraded by the alcohol dehydrogenase while it is first converted into acetaldehyde and then into acetic acid, a metabolite thereof. If this metabolism does not occur in a calm manner, the accumulated ethyl alcohol or the converted acetaldehyde during the process of alcohol degradation will lead to the processing of toxins, causing damage to the cells of the liver and brain, so that metabolic diseases can occur in the body, causing total fatigue of the body, abdominal distension, eme headache, and even abdominal cramps and pain, resulting in hangovers.

Such hangovers can make everyday life abnormal and cause a lack of energy, which has severe effects not only on the person who finds the hangover itself but also on other people. Therefore, such hangovers incur significantly negative results of daily life. Since the earliest times, different foods have been used in home remedies for the relief of such hangovers. That is, foods such as ox blood soup, bean soup and brussel sprouts, dried Pollack soup, seafood soups, oysters or juices, such as Aegopodium podagraria L., radishes, cucumbers, leeks, spinach, roots of lotus, arrowroot, pine needles juice, ginseng juice and green tea leaves, have been considered effective in relieving hangover. Particularly, it has been considered that green tea leaves have been highly effective in relieving hangovers, since they contain phenol and therefore, can easily decompose acetaldehyde. In addition, beverages have been developed and sold to alleviate such hangovers. For example, Korean Patent No. 0181168 describes a natural tea for relieving hangovers and a method for preparing it. The natural tea prepared according to this Korean Patent contains an extract of, or a powder of the leaf, stem or root of Alnus japonica and Sorbus commixta as an active component, as well as certain amounts of extracts of the fruits of Ligustrum japonicum Thunberg and the root of Pueraria. Also, the pending Korean Patent Application No. 2001-019767 describes a natural tea for relieving hangovers and the method for preparing it. The natural tea prepared in accordance with this Korean Patent Application contains materials of Chinese medicine, including Pueraria flowers, Pueraria roots, Liquordice roots, Rhizome White Atractylodes, dried leaf husks, Rhizoma Alismatis, Lycium chínense and ginger. By means of said methods, several beverages have been developed to alleviate the hangover, each having a unique characteristic. However, despite various efforts to relieve hangovers, there is still a need for the development of beverages or foods that can effectively relieve hangovers. SUMMARY OF THE INVENTION Accordingly, an object of the present invention is to provide natural plants that have excellent effects for relieving hangovers and revitalizing liver functions and for producing a natural tea that has excellent effects for the relief of hangovers and revitalization. of liver functions, rapidly decreasing the concentration of alcohol when it is taken before or after drinking alcohol, in which natural tea contains an extract of these natural plants as the main active ingredient. Another object of the present invention is to provide a natural tea, which has the effects of relieving hangovers and revitalizing liver functions, which allows the human body to be maintained under normal conditions before or after generation of a hangover, thereby providing health benefits. Still another object of the present invention is to provide a method for preparing the natural tea according to the above objects. To achieve the above objects, the present invention provides a natural tea, which has the effects of relieving the hangover and revitalizing liver functions and which comprises an extract of pseudo-acacia Robinia as the main active ingredient and conventional additives . It should be understood that the pseudo-acacia extract, Robinia, which is used in the present invention, includes all parts of the pseudo-acacia Robinia, including the leaf, the stem, the flower, the root and the pseudo-fruit. acacia Robinia. In natural tea according to another embodiment of the present invention, the extract of the leaves, the stem, the flower and the pseudo-acacia Robinia root are contained as the main active ingredient, to which at least one can be added an extract selected from Hedyotis diffusa, Amomi semen, Glycyrrhiza and Pueraiae Flos as a minor active component. However, the present invention is achieved by the main active component and said minor active components are optional and not critical to the present invention. In another embodiment, the present invention provides a natural tea that has the effects of relieving the hangover and revitalizing the functions of the liver, which comprises a mixture of 10% to 80% by weight of an extract of pseudo-acacia Robinia, and of the 20% to 90% by weight of Hedyotis diffusa. In yet another embodiment, the present invention provides a natural tea having effects of relieving hangovers and revitalizing liver functions, which comprises a mixture of 10% to 65% by weight of an extract of pseudo-acacia Robinia , and from 20% to 80% by weight of Hedyotis diffusa, and from 10% to 60% by weight of Amomi semen, and from 10% to 50% by weight of Glycyrrhiza. Also, the present invention provides a method for preparing natural tea that has effects of relieving hangovers and revitalizing liver functions, the method comprising the steps of: washing the leaves, stem, flower and pseudo-root acacia Robinia with water; extracting the washed material with water with alcohol as an extraction solvent at a temperature of between 50 to 150 ° C for a period of 1 to 10 hours to prepare the pseudo-acacia extract Robinia as the main active component; and add conventional additives to the extract. In the method according to the present invention, the extraction step is preferably carried out at a temperature of 50 ° C to 150 ° C. If the extraction temperature is below 50 ° C, the active ingredients of pseudo-acacia Robinia will not be easily extracted. If the extraction temperature is higher than 150 ° C, the extraction effect will not be increased and the active ingredients can be modified. The extraction step is carried out in a more preferred manner at a temperature of 50 ° C to 90 ° C. In another embodiment of the present invention, the extract is centrifuged at a speed of 2,500 to 7,500 rpm to separate the robinia. In yet another embodiment of the present invention, each component is dried in the shade and sprayed to a mesh size of 50 to 100, so that a powdered tea is provided. The acacia, which is used as the main component in the present invention, is a plant belonging to the Leguminosae family of the order of Rosales, dicotyledonous plants. There are approximately 500 species of acacia in temperate and tropical areas of the world, particularly in Australia. Its leaves are leaves of a compound of double paripinato, each having very small leaves and a peptiolo that is flat or similar to a leaf. Also, its flowers have a yellow or white color, and an acacia is often designated as a pseudo-acacia Robinia that is also native to North America. The Hedyotis diffusa, which is used in the present invention, is an annual herb belonging to the Rubiaceae family that grows at a height of about 30 cm. It is native to China, but is also found on Mt. Halla on Jejudo Island or on the marshy land of Baekun Mountain in Jeolla-Namdo in the Republic of Korea. This can be considered as a newly discovered medicinal herb not described in the ancient books of Chinese medicine, such as the Compendium of Materia Medica. It has been used in research and experiments in China for 40 years, when it was first described in the Guangxi Journal of Traditional Chinese Medicine just after the year 1945. The Hedyotis diffusa has been widely known in the world, since its effectiveness and action were described in detail in the book "Traditional Chinese Medicine Research" published by the Taiwan Center of Traditional Chinese Medicine. The Glycyrrhiza, which is used in the present invention, is a perennial plant, which belongs to the family of Leguminosae of the order of Rosales, which are dicotyledonous plants. Its root has a reddish brown color and penetrates deep into the earth. Its stem is angular and grows straight at a height of about 1 meter. The stem has white fluff distributed close to it and shows a clear ash color. Also the stem has pellucid spots scattered on it. The leaf is a sheet composed of alternate oddipinato, of 1 to 17 leaves, which are similar to the eggs in a flat and final form. Each of the leaves has a length of 2 to 5 cm. and a width of 1 to 3 cm., has white fluff and lint spots on both sides, and has no serratos. Examples of the Glycyrrhiza include G. glabra var. glandulifera, native to Siberia, and G. glabra, native to Spain. And similar species include G. pallidiflora. Pueraiae Flos, which is used in the present invention, refers to a Pueraria flower in Chinese medicine and has been mainly used as a drug to alleviate alcoholic poisoning and to stop its discharge into the blood. The natural tea of the present invention prepared as described above has the effects of relieving hangovers and revitalizing liver functions. This natural tea comprises, either an extract of, or a powder from the leaf, stem, flower and root, of pseudo-acacia Robinia distributed in the East, as the main active component, to which at least one selected from extracts of Hedyotis diffusa, Amomi semen, Glycyrrhiza, and Pueraiae Flos, to neutralize the poison in a certain amount. When taken before or after drinking alcohol, the natural tea of the present invention shows excellent effects of relieving hangovers and revitalizing liver functions. Brief Description of the Drawings Figure 1 is a graphic diagram showing a change in body weight in the experiment that was used during a trial period; Figure 2 is a graph showing an effect of long-term alcohol administration on the hematocrit content; and Figure 3 is a graphic diagram showing an effect of long-term alcohol administration on the hemoglobin content. Detailed Description of the Invention Hereinafter, the present invention will be described in more detail by means of the test examples. However, it should be kept in mind that the present invention is not limited to, or by these examples. Example 1: Preparation of acacia extract The leaf, stem, flower and pseudo-acacia Robinia root were selected, and washed with water. A certain amount of the material washed with water was extracted with alcohol at a temperature of about 70 ° C for 5 hours, to produce the acacia extract. Example 2 Acacia extract prepared according to Example 1 was used as the main active component, to which were added conventional additives, such as a sweetening agent, spices and a coloring agent, thereby preparing a natural tea having the effects of relieving hangovers and revitalizing liver functions. Example 3 Natural tea having effects of relieving hangovers and revitalizing liver functions was prepared in the same manner as in Example 2, except that a mixture of 80% by weight of acacia extract and 20% by weight was used. weight of extract of Hedyotis diffusa. Example 4 Natural tea having effects of hangover relief and revitalization of liver functions was prepared in the same manner as Example 2, except that a mixture of 70% by weight of acacia extract and 30% was used in weight of an extract of Hedyotis diffusa. Example 5 Natural tea having effects of relieving hangovers and revitalizing liver functions was prepared in the same manner as in Example 2, except that a mixture of 60% by weight of acacia extract and 40% was used in weight of extract of Hedyotis diffusa. Example 6 Natural tea having effects of hangover relief and revitalization of liver functions was prepared in the same manner as in Example 2, except that a mixture of 50% by weight of acacia extract and 50% was used. in weight of extract of Hedyotis diffusa. Example 7 Natural tea having effects of hangover relief and revitalization of liver functions was prepared in the same manner as in Example 2, except that a mixture of 40% by weight of acacia extract and 60% was used. in weight of extract of Hedyotis diffusa. Example 8 Natural tea having hangover relief effects and revitalization of liver functions was prepared in the same manner as in Example 2, except that a mixture of 30% by weight of acacia extract and 70% was used in weight of extract of Hedyotis diffusa.

Example 9 Natural tea that has effects of hangover relief and revitalization of liver functions was prepared in the same manner as in Example 2, except that a mixture of 20% by weight of acacia extract and 80% was used. in weight of extract of Hedyotis diffusa. Example 10 Natural tea having effects of hangover relief and revitalization of liver functions was prepared in the same manner as in Example 2, except that a mixture of 10% by weight of acacia extract and 90% was used. in weight of extract of Hedyotis diffusa. Examples from 11 to 36 Natural teas that have effects of relieving hangovers and revitalization of liver functions were prepared in the same manner as Example 2, except that extracts of pseudo-acacia Robinia, Hedyotis diffusa, Amomi semen were mixed , Glycyrrhiza, and / or Pueraiae Flos among them, in a mixing ratio determined in Table 1 below.

Table 1 (unit:% by weight) Test Example 1: Change in body weight Figure 1 shows a change in body weight during a test period. Until certain weeks after the start of the test, three test groups except control group B were given only ethanol in order to cause liver damage. At the start of the trial, the body weights were between 278.9 ± 13.7 g, 261.6 ± 22.0 g, 260.6 ± 23.6 g and 263.7 ± 13.1 g for groups A, B, C and D, respectively. The body weight of the groups that were administered alcohol was about 17 g greater than the control group. Control group B, which was not administered alcohol and beverages, showed a greater increase in body weight. Despite long-term ethanol administration, group A that was only given alcohol showed a continuous increase in body weight during the trial period compared to the other two groups C and D that were given drinks. of relief from the hangover. Presumably, this is due to the fact that the body weight of group A at the time of the start of the tests was higher than the other two groups C and D. The groups that were administered the drink for the relief of the hangover showed an increase in body weight up to three weeks after the start of the test without a significant difference between the groups. However, after week three, group D, the group that was administered the natural tea of the present invention, showed a clear increase in body weight compared to group C to which they were given relief drinks of the usual hangover in the market. Example 2: Contents of hematocrits and hemoglobin Figure 2 and Figure 3 show the contents of hematocrit (Ht) and hemoglobin (Hb), respectively, which were measured at the end of the test. There was no significant difference in the Ht content between the treated groups. However, group D, to the group that was administered the natural tea of the present invention, showed a higher Ht content of 39.8%. Control group B and group C, the group that was given the usual hangover relief drink on the market, showed an Ht content of 38.0%, and group A, which was only given alcohol, showed a somewhat lower content of 37.5% Ht. The Hb contents were significantly statistically different between the treated groups (p <0.05). The control group showed the highest Hb content of 17.38 g / dL, the C group that was administered the commercial hangover relief drink showed 16.41 g / dl, and the D group to which the natural tea was administered of the present invention showed 15.77 g / dl, and group A to which only alcohol was administered showed 14.83 g / dl. Example 3: Lipid content in serum Table 2 below shows the lipid content in the serum measured at the end of the test. Group B showed the highest triglyceride (TG) content of 76.0 mg / dL. Group C to which the commercial hangover relief drink was administered and group D to which the natural tea was administered showed substantially similar TG contents of 53.6 mg / dL and 49.0 mg / dL, respectively. The total cholesterol (TC) content in a range of 121.2-127.6 mg / dl with no difference between the treated groups.

With respect to high density lipoprotein (HDL) cholesterol, group D that was administered the natural tea of the present invention showed the highest value of 39.6 mg / dl, and group C to which the drink was administered commercial showed the lowest value of 31.3 mg / ml (p <0.05). The content of low density lipoprotein (LDL) cholesterol was not different between the test groups, but the group that administered the commercial beverage product showed a somewhat higher value of 80.2 mg / dL. The atherogenic index (Al), which is a risk factor for diseases in adults, was 2.14 in group D to which the natural tea of the present invention was administered, which is statistically significantly lower than the other groups treated (p <0.05). Table 2: Effect of long-term alcohol administration on serum lipid concentration (mean ± SD).

* TG: triglycerides TC: total cholesterol HDL: high-density lipoprotein cholesterol LDL: low density lipoprotein cholesterol atherogenic index = (HDL-TC) / HDL The groups that are given an indication with different letters showed an important difference between the average values (p < 0.05). Test example 4: Liver function test Table 3 below shows the effect of long-term alcohol administration on liver functions. Bilirubin, ALP, GOT and r-GTP have a significantly different content among the treated groups (p <0.05). The bilirubin content was 3.54 mg / dl, the highest value, in group B, and 1.21 mg / dl, the lowest value, in group D to which the natural tea of the present invention was administered. The activities of alkaline phosphatase (ALP) were of unit of 17.7 KA, the highest value in the administration group only of alcohol A, and to 11.9 unit KA, the lowest value, in group D to which the tea was administered natural. The glutamic oxaloacetic transaminase (GOT) activities were 77.4 IU / L, the highest value, in group A that was only given alcohol, and 60.7 IU / L, the lowest value in group D that was He gave her natural tea. The GPT activities were not significantly different between the treated groups but were somewhat lower in the D group to which the natural tea of the present invention was administered. The content of transpeptidase? -glutamilo (? -GTP) which are used to evaluate the fatty alcoholic liver was 127.6 mU / ml, the highest value, in the group C to which the hangover relief drink was administered. commercial, and 122.6 mU / ml, the lowest value, group A, which was only administered alcohol, which was statistically lower than group C, which was administered to the commercial product (p <0.05). Meanwhile, lactate dehydrogenase (LDH) activities were not significantly different between the treated groups. Control group B showed the highest LDH activity of 398.0 μ / L, and group D that was administered the natural tea of the present invention showed the lowest LDH activity of 339.7 μ / L. Table 3: Effect of long-term alcohol administration on liver functions.

ALP: alkaline phosphatase (* KA unit: King-Armstrong unit) GOT: glutamic oxaloacetic transaminase GPT: glutamic pyruvic transaminase? -GTP: transpeptidase r-glutamyl LDH: lactate dehydrogenase Indications with different letters have shown important differences between the values average (p <0.05). Test example 5: Concentrations of ethanol and acetaldehyde in the blood and alcohol dehydrogenase activity (ADH) As shown in Table 4 below, the total assimilation of alcohol was zero in group B without the administration of alcohol and beverages of relief from the hangover. Total alcohol administrations were 28.4 g in group A alcohol alone, 26.4 g in group C given the commercial hangover relief drink, and 27.1 g in group D who received the tea. natural of the present invention. Therefore, the total assimilations of alcohol were statistically different between the groups treated with ethanol (p <; 0.05). The concentrations of ethanol in the blood, which had been measured after the long-term administration of alcohol were 0.015%, 0.010%, 0.004% and 0.003% as averages in group C to which the relief drink was administered. of the commercial hangover, group A that was administered only alcohol, control group B and group D to which the natural tea of the present invention was administered, respectively (p <0.05). Meanwhile, acetaldehyde concentrations in the blood were 82.6 ± 36.2 μg / dl, 39.8 ± 44.0 μg / dl, and 32.2 ± 35.6 μg / dl in the group given the commercial hangover relief drink, the group that was administered natural tea of the present invention, the control group, respectively. Therefore, the concentration of acetaldehyde in the blood in the group to which the natural tea of the present invention was administered is lower than in the group to which the commercial hangover relief beverage was administered, but similar to the control group. Also, the concentration of acetaldehyde in blood in group A to which only alcohol was administered was 222.3 ± 85.8 μg / dl which is much higher than the other three groups. The alcohol dehydrogenase activity (ADH) was 17.98 nmol NADH / min / mg protein, the highest value, in the D group to which the natural tea of the present invention was administered. ADH activities were 16.8 nmol NADH / min / mg protein in group C to which the commercial hangover relief drink was administered, 15.55 nmol NADH / min / mg protein in control group B, and 15.00 nmol NADH / min / mg of protein in group A to which alcohol was only administered. Therefore, group D to which the natural tea of the present invention was administered showed a somewhat higher ADH activity than the other three groups.

Table 4: Assimilation of alcohol during the trial period, alcohol and acetaldehyde concentrations in the blood and ADH activity (mean ± SD). (n = 9, mean ± SD) * ADH: alcohol dehydrogenase (nmol NADH / min / mg protein) indications with other letters have shown an important difference between the average values (p <0.05). In Test Examples 1 to 5, the alcohol was administered to white rats for a long period (10 weeks), and the efficiencies of alcohol degradation in the commercial product were examined as a hangover relief beverage and the natural tea in the present invention. From the previous results reported, the following can be concluded; 1) The growth rates were somewhat higher in the control group, but not statistically different between the treated groups. The hematocrit (Ht) values were higher in the natural tea group than in the other groups, but not statistically different between the treated groups. The hemoglobin contents were statistically different between the treated groups (p < 0.05). 2) The contents of triglycerides (TG) and HDL cholesterol and atherogenic indices were significantly different between the treated groups (p <0.05). The TG content was the lowest for the group that was given the natural tea while the HDL cholesterol content was the highest for the group that was given the natural tea. 3) The liver factors (bilirubin, ALP, GOT and y-GTP) were significantly different between the treated groups (p <0.05). The bilirubin contents were 3.54 mg / dl, the highest value in the control group, and 1.21 mg / dl, the lowest value in the group given the natural tea of the present invention. The ALP activities were 17.7 unit K-A, the highest value in the group that was only administered alcohol, and 11.9 unit K-A, the lowest value in the group to which the natural tea of the present invention was administered. The GOT contents were 77.4 IU / L, the highest value in the group that was only given alcohol, and 60.7 IU / L, the lowest value in the group to which the natural tea was administered. Nvention The content of? -GTP, which is used to evaluate the fatty alcoholic liver were higher, in the order of the administered group of the commercial hangover relief tea, the group that was administered only alcohol and the group to which it was administered. he administered the natural tea of the present invention and the control group. 4) The concentrations of ethanol in the blood were 0.003%, the lowest value, the group to which the natural tea was administered (p <0.05), and the concentrations of acetaldehyde in the blood were 82.6 ± 36.2 μg / dl and 39.8 ± 44.0 μg / dl in the group to which the commercial hangover relief drink was administered and to the group to which the natural tea was administered, respectively. Therefore, the concentration of acetaldehyde in the blood was lower in the group given the natural tea than in the group given the commercial hangover relief drink. Meanwhile, the activities of alcohol dehydrogenase did not have an importance between the treated groups but it was higher in the group to which the natural tea was administered than in the other groups. To summarize, the alcohol was administered orally to white rats for a long period (10 weeks) in an amount sufficient to cause alcoholic damage to the liver after which a natural tea of the present invention was administered (Dawn-808), a hangover relief drink to white rats from week three before the end of the test. In this case, the group of white rats given the natural tea of the present invention showed excellent serum lipid concentration and liver functions compared to the other groups. further, in the group to which the natural tea was administered, the ADH activity in liver tissue was somewhat higher than in the other groups and the alcohol concentration was significantly lower than in the other groups. This suggests, that the natural tea of the present invention has an effect of promoting the metabolism of alcohol. Test Example 6: Change in acetaldehyde and alcohol concentrations in blood Tables 5 and 6 below show blood alcohol concentrations measured in the first and second clinical tests, respectively. In the first clinical trial, the test volunteers after an occupation took at least one bottle of Soju (distilled liquor). Table 5 shows the concentrations of alcohol in the blood along with the acetaldehyde concentrations in the blood taken at 12:00 noon (9:00 AM the next day) after drinking alcohol. Blood alcohol concentrations measured with a breathalyzer were not statistically significant between a test group and a control group, but were lower in the test group that took the natural tea of the present invention than in the test group over time. The concentration of alcohol in the blood measured at 12:00 hours after drinking alcohol were 0.006% and 0.030% in the test group, and the control group, respectively. Meanwhile, the blood acetaldehyde concentrations measured at the same time as the blood alcohol concentrations of the test group were reduced to a lower level of 1/2 than in the control group. Such results generally coincide with the response of volunteers in the test group that the natural tea of the present invention helped to relieve hangover the next day after drinking alcohol. Table 5: Effect of natural tea on the concentrations of alcohol and aldehyde in the blood. (first clinical trial) (average ± SD). Table 6 shows the results of a second clinical trial. In the second clinical trial, the university students took a bottle of Passport®, a brand of Korean whiskey, and it was forbidden to drink additional beverages. The concentrations of alcohol in the blood were lower than those of the first clinical trial where the test volunteers took at least one bottle of soju, a type of Korean alcoholic beverage. Presumably, the results of the second clinical trial were in this way because the amount of alcohol consumed was lower and the age of the test volunteers was lower than the volunteers of the first clinical trial. As with the first clinical trial, the test group to which the natural tea of the present invention was administered showed lower concentrations of alcohol in the blood than the test group, except for a point of 30 minutes after drinking alcohol. . The concentrations of alcohol in the blood in the 360 minutes after drinking alcohol were 0.016% in the test group and 0.033% in the control group, indicating a large difference in blood alcohol concentrations between the two groups . Meanwhile, the concentrations of aldehyde in the blood were measured at 60 minutes, 150 minutes and 240 minutes after drinking alcohol and the results are given in Table 7 below. The concentration of acetaldehyde in the blood at 60 minutes after drinking alcohol did not matter between the two groups, but was much smaller in the test group than in the control group. This difference in the concentration of acetaldehyde in the blood was reduced after 150 minutes and 240 minutes after drinking the alcohol, the concentration of aldehyde correlated with the blood concentration. Table 6: Effect of natural tea on the concentration of alcohol in the blood (second clinical test) (mean ± SD) Table 7: Effect of natural tea on the concentration of acetaldehyde in the blood (second clinical test) (mean ± SD) Test Example 7: Change in liver function activity Table 8 shows a change in liver function activity of blood collected from the second clinical trial. The values of GOT and GPT are numerical for the enzymes which are transferred from the cells of the liver to the blood, when the cells of the liver are broken due to the inflammation in the liver. A GOT which indicates the degree of rupture of liver cells was lower in the test group than in the control group over time. Particularly, 150 minutes after drinking alcohol, the GOT was significantly lower in the test group than in the control group (p <0.05). ? -GTP, which is used to evaluate fatty liver and alcoholic liver disease, was higher in the test group than in the control group at 0 minutes after drinking alcohol. However, the? -GTP in the test group was then reduced and reached a value similar to that of the control group at 240 minutes after drinking alcohol. Table 8: Effect of natural tea on liver functions (mean ± SD) ** (p < 0.05) GOT & GPT (Kamen unit)? -GTP (mU / ml) Test Example 8: Lipid change in serum Table 9 below shows the contents of triglycerides (TG) and total cholesterol (TC).

The content of TG in the blood was lower in the test group than in the control group at any time during the measurement time. This seems to be attributable to a difference between the TG content between the two groups at the time of the start of the measurement. However, the TG content in the serum in the control group reached the highest value of 354.3 mg / dL at 150 minutes after drinking alcohol and then was reduced by approximately 100 mg / dL at 240 minutes after drink. In both of the two groups, the TG contents were higher at 240 minutes than at minute 0. The TC concentrations in the blood were reduced in both of the two groups over time after drinking alcohol. Table 9: Effect of natural tea on serum lipid content (mean ± SD) TG: triglycerides TC: total cholesterol Test Example 9: Change in the activity of ADH in the serum The alcohol dehydrogenase activity that has a determining effect on the degradation of alcohol follows in order of zero reaction where the alcohol is metabolized only in a constant amount per unit of time regardless of the concentration of alcohol in the cells. Therefore, the activity of this enzyme is constant regardless of the degree to which alcohol has been taken (Takagi et al., 1985, and Oshii et al., 1973). Table 10 below shows a change in ADH activity over time. The ADH activities measured at 0 minutes after drinking alcohol were 2.63 U / L and 3.53 U / L in the test group and the control group, respectively, indicating that the ADH activity of the control group was more high that the test group, but did not have a significant statistical difference with the test group. However, with respect to a change in ADH activity over time, the test group showed an ADH activity of 4.12 U / L at 60 minutes after drinking alcohol, indicating an increase of more than 50 % in ADH activity compared to 2.63 U / L in minute 0. Also, the test group showed an ADH activity of 3.76 U / L at 150 minutes, and an ADH activity of 3.09 U / L at 240 minutes, which is approximately 17% higher than the ADH activity at minute 0 after drinking alcohol. On the other hand, the control group, measured values of ADH activity in the 150 minutes and 240 minutes were somewhat inconstant, but ADH activity at minute 0 after drinking alcohol over time reduced by more of 20% at 240 minutes after drinking alcohol. Table 10: Effect of natural tea on ADH activity (mean ± SD) As can be seen in Table 10, although there is no statistical difference between the two groups, the test group which ingested the natural tea of the present invention showed a somewhat higher activity of ADH than the control group. This suggests that the natural tea of the present invention promoted the metabolism of alcohol in the blood. In Test Examples 6 to 9 above, the test volunteers were given the natural tea of the present invention before or after drinking the alcohol, and was measured by their alcohol degradation capacity in an experimental design of crossing. The results of the test provide the following possible conclusions. 1) The concentrations of alcohol in the blood at all time points of measurement were lower in the group to which the natural tea of the present invention was administered than in the control group, but did not have statistical significance among the two groups. Also, acetaldehyde concentrations in the blood were lower in the test group than in the control group. 2) With respect to the activity of liver function, the concentration of GOT was lower in the test group than in the control group over time. Particularly, at 150 minutes after drinking alcohol, it became a statistical significance between the two groups (p <0.05). The concentrations of GPT and? -GTP reached the highest values at 60 minutes after drinking alcohol and then declined. The concentrations of GPT and? GTP were somewhat lower in the test group to which the natural tea was administered than in the control group. 3) With respect to the lipid content in the serum, the content of triglycerides (TG) was higher in the control group than in the test group. Particularly, in the control group, TG content reached the highest value at 150 minutes and then declined by approximately 100 mg / dL at 240 minutes. In both of the two groups, the TG content at 240 minutes was higher than at minute 0.

Total cholesterol (TC) content was reduced in both groups over time after drinking alcohol. 4) Meanwhile, a survey of questions was carried out in the volunteers who participated in the clinical trials, and they answered that the hangover syndromes, such as headache, asthenia and heartburn, which they usually experienced the following day after After drinking the alcohol, they were relieved after drinking the natural tea of the present invention. As described above, the present invention provides the liquid natural powder tea comprising a pseudo-acacia extract of Robinia, and optionally, a certain amount of extracts of Hedyotis diffusa, Amomi semen, Glycyrrhiza and / or Pueraiae Flos, as well as the method to prepare it. The natural tea according to the present invention shows the effects of relieving hangovers and protecting the liver when it is taken before or after drinking alcohol. Particularly, according to the present invention, the robinin extracted from the Robinia pseudo-acacia can be used as a single active ingredient in a hangover relief beverage.

Claims (16)

1. CLAIMS 1. A natural tea that has the effect of relieving hangovers and revitalizing the functions of the liver, the natural tea comprising a pseudo-acacia extract of Robinia as the main active ingredient and conventional additives.
2. 2. The natural tea as described in claim 1, which additionally comprises at least one selected from extracts of Hedyotis diffusa, Amomi semen, Glycyrrhiza and Pueraiae Flos, as the minor active ingredients.
3. 3. The natural tea as described in claim 1 or 2, which comprises a mixture of 10% to 80% by weight of Robinia pseudo-acacia extract, and 20% to 90% by weight of extract of Hedyotis diffusa.
4. 4. The natural tea as described in claim 2, which comprises a mixture of (i) 10% to 65% by weight of pseudo-acacia extract of Robinia, (ii) from 20% to 80% in weight of extract of Hedyotis diffusa, and (iii) from 10% to 60% by weight of Amomi extract semen or from 10% to 50% by weight of Glycyrrhiza extract.
5. 5. The natural tea as described in claim 2, which is prepared by extracting from 10% to 30% or, by weight of Robinia pseudo-acacia, from 20% to 60% by weight of Hedyotis diffusa, and from 10% to 40% by weight of Amomi semen, from 10% to 20% by weight of Glycyrrhiza and from 10% to 30% by weight of Pueraiae Flos, with an extraction solvent.
6. 6. A natural powdered tea which has the effects of relieving hangovers and revitalizing the functions of the liver, natural powder tea being prepared by washing the leaf, stem, flower and pseudo-acacia root of Robinia with water, drying the washed material with water in the shade, and spraying the dry material to a mesh size of 50 to 100.
7. 7. The natural tea powder as described in claim 6, which additionally comprises, a powder of mesh size from 50 to 100 of at least one selected from Hedyotis diffusa, Amomi semen, Glycyrrhiza and Pueraiae Flos.
8. 8. The natural powdered tea as described in claim 7, which is prepared by spraying (i) from 10% to 65% by weight of Robinia pseudo-acacia, (ii) from 20% to 80% by weight. weight of Hedyotis diffusa, and (iii) 10% to 60% by weight of Amomi semen, or 10% to 50% by weight of Glycyrrhiza, of a uniform mesh size of 50 to 100.
9. 9. The natural tea in powder as described in claim 7, which is prepared by spraying from 10% to 30% by weight of Robinia pseudo-acacia, from 20% to 60% by weight of Hedyotis diffusa, from 10% to 40% by weight weight of Amomi semen, from 10% to 20% by weight of Glycyrrhiza, and from 10% to 30% by weight of Pueraiae Flos, a uniform size of meshes of 50 to 100.
10. 10. A method to prepare a natural tea that has the effects of relieving hangovers and revitalizing the functions of the liver, the method comprising the steps of: washing the leaves, the stem, and the flower and the pseudo-acacia root of Robinia with water; extracting the washed material with water with an alcohol extraction solvent at a temperature of 50 ° C to 150 ° C for a period of 1 to 10 hours to produce an extract that is to be used as an active ingredient; and add conventional additives to the extract.
11. The method as described in claim 10, which further comprises adding at least one minor active ingredient selected from extracts of Hedyotis diffusa, Amomi semen, Glycyrrhiza and Pueraiae Flos, to the pseudo-acacia extract of Robinia.
12. The method as described in claim 10 or 11, characterized in that from 10% to 80%, by weight of pseudo-acacia extract of Robinia, and from 20% to 90% by weight of extract Hedyotis diffusa are mixed among them.
13. The method as described in claim 11, characterized in that (i) 10% to 65% by weight of Robinia pseudo-acacia extract are mixed together (i) from 20% to 80% by weight of Hedyotis diffusa extract, and (iii) from 10% to 60% by weight of Amomi semen extract or from 10% to 50% by weight of Glycyrrhiza extract.
14. 14. The method as described in claim 11, characterized in that 10% to 30% by weight of Robinia pseudo-acacia are mixed and extracted with the extraction solvent from 20% to 60% by weight of Hedyotis diffusa, from 10% to 40% by weight of Amomi. semen, from 10% to 20% by weight of Glycyrrhiza and from 10% to 30% by weight of Pueraiae Flos.
15. The method as described in claim 10, which additionally comprises centrifugation of the Robinia pseudo-acacia extract at a speed of 2,500 to 7,500 rpm to separate the robinin for use.
16. 16. The method as described in any of claims 10, 13 and 14, which further comprises centrifuging the extracts at a rate of 5000 to 7500 rpm to separate the active ingredients for use.